公开(公告)号：US20140346344A1

公开(公告)日：2014-11-27

申请号：US14274707

申请日：2014-05-10

Applicant: Academia Sinica

Inventor： Chung-Hsuan Chen ,  Jung-Lee Lin ,  Huan Chang Lin

IPC: G01N27/66 ,  H01J49/42 ,  G01N33/483 ,  H01J49/36

CPC classification number: H01J49/36 ,  G01N33/4833 ,  H01J49/164 ,  H01J49/4225 ,  H01J49/424

Abstract: Apparatus and methods for performing mass spectrometry of a nanoparticle or virus analyte. Apparatus may include a laser desorption plate, a mass analyzer configured to measure mass over the range of m/z from 105 to 1010, an electrical shield surrounding the mass analyzer, and a charge sensitive detector, wherein the laser firing is phase lock synchronized with the applied radiofrequency voltages.

Abstract translation: 用于进行纳米颗粒或病毒分析物的质谱分析的装置和方法。 设备可以包括激光解吸板，质量分析器，被配置为测量从105到1010的m / z范围内的质量，围绕质量分析器的电屏蔽，以及电荷敏感检测器，其中激光点火与 应用的射频电压。

公开(公告)号：US08887574B2

公开(公告)日：2014-11-18

申请号：US13314957

申请日：2011-12-08

Applicant: Yi-Chung Tung ,  Chueh-Yu Wu ,  Wei-Hao Liao

Inventor： Yi-Chung Tung ,  Chueh-Yu Wu ,  Wei-Hao Liao

IPC: G01L9/02 ,  G01L9/00 ,  B01L3/00

CPC classification number: G01L9/0058 ,  B01L3/5027 ,  Y10T29/49155

Abstract: A measurement system for measuring a pressure of a fluid is provided. The measurement system includes a sensing module and a liquid electrical circuit. The sensing module includes a flexible film and a liquid electronic device. The flexible film has a first side and a second side opposite to the first side. The fluid is disposed on the first side of the flexible film, and the liquid electronic device is disposed on the second side of the flexible film. The flexible film converts the pressure of the fluid into a parameter of the liquid electronic device. The liquid electrical circuit is electrically coupled to the liquid electronic device. The liquid electronic device and the liquid electrical circuit output a measurement signal corresponding to the parameter in response to an applied electrical energy. A manufacture method of a measurement system is also provided.

Abstract translation: 提供了用于测量流体压力的测量系统。 测量系统包括感测模块和液体电路。 感测模块​​包括柔性膜和液体电子装置。 柔性膜具有与第一侧相对的第一侧和第二侧。 流体设置在柔性膜的第一侧上，并且液体电子器件设置在柔性膜的第二侧上。 柔性膜将流体的压力转换成液体电子装置的参数。 液体电路电耦合到液体电子装置。 液体电子装置和液体电路响应于所施加的电能输出对应于参数的测量信号。 还提供了测量系统的制造方法。

公开(公告)号：US08858776B2

公开(公告)日：2014-10-14

申请号：US13170624

申请日：2011-06-28

Applicant: Lain-Jong Li ,  Ching-Yuan Su

Inventor： Lain-Jong Li ,  Ching-Yuan Su

IPC: C25B1/00 ,  B82Y40/00 ,  B82Y30/00 ,  C01B31/04

CPC classification number: B82Y30/00 ,  B82Y40/00 ,  C01B32/19 ,  C01B2204/32 ,  C25B1/00 ,  Y10S977/845

Abstract: A method of preparing graphene sheets. The method includes: immersing a portion of a first electrode and a portion of a second electrode in a solution containing an acid, an anionic surfactant, a salt, an oxidizing agent, or any combination thereof as an electrolyte, the immersed portion of the first electrode including a first carbon material and the immersed portion of the second electrode including a second carbon material or a metal; causing a potential to exist between the first and second electrodes; and recovering, from the solution, graphene sheets exfoliated from the carbon material(s). Also disclosed is a method of preparing a graphene film electrode. The method includes: dissolving graphene sheets in an organic solvent to form a solution, applying the solution on a substrate, adding deionized water to the solution on the substrate so that a graphene film is formed, and drying the graphene film.

Abstract translation: 制备石墨烯片的方法。 该方法包括：将第一电极和第二电极的一部分浸入含有酸，阴离子表面活性剂，盐，氧化剂或其任何组合的溶液中作为电解质，第一 包括第一碳材料的电极和包括第二碳材料或金属的第二电极的浸没部分; 导致在第一和第二电极之间存在潜力; 并从溶液中回收从碳材料剥离的石墨烯片。 还公开了制备石墨烯膜电极的方法。 该方法包括：将石墨烯片溶解在有机溶剂中以形成溶液，将溶液涂布在基材上，向基板上的溶液中加入去离子水，形成石墨烯膜，并干燥石墨烯膜。

公开(公告)号：US20140279758A1

公开(公告)日：2014-09-18

申请号：US14215163

申请日：2014-03-17

Applicant: ACADEMIA SINICA

Inventor： An-Suei Yang ,  Hung-Pin Peng ,  Jhih-Wei Jian

IPC: G06F19/12 ,  G06F17/30 ,  G06F19/24

CPC classification number: G16B5/00 ,  G16B40/00 ,  G16B50/00

Abstract: In a general aspect, a method for inferring one or more biomolecule-to-biomolecule interaction sites includes receiving data representative of a plurality of prediction models. Each prediction model is associated with a different atom type of a plurality of atom types and characterizes biomolecule-to-biomolecule interaction site specific patterns common to a plurality of three dimensional probability density maps. Each three dimensional probability density map is associated with a corresponding biomolecule of a plurality of biomolecules included in a training data set and represents a probability of a non-covalent interacting atom on a surface of the corresponding biomolecule interacting with the atom type associated with the prediction model. Data representative of a query biomolecule is received, the data including one or more unknown biomolecule-to-biomolecule interaction sites. The one or more unknown biomolecule-to-biomolecule interaction sites of the query biomolecule are inferred based on the data representative of the plurality of prediction models.

Abstract translation: 在一般方面，用于推断一个或多个生物分子与生物分子相互作用位点的方法包括接收表示多个预测模型的数据。 每个预测模型与多个原子类型的不同原子类型相关联，并且表征多个三维概率密度图所共有的生物分子对生物分子相互作用位点特异性模式。 每个三维概率密度图与包括在训练数据集中的多个生物分子的相应生物分子相关联，并且表示在与预测相关的原子类型相互作用的相应生物分子的表面上的非共价相互作用原子的概率 模型。 收到代表查询生物分子的数据，该数据包括一个或多个未知生物分子与生物分子的相互作用位点。 基于代表多个预测模型的数据，推断查询生物分子的一个或多个未知的生物分子对生物分子相互作用位点。

公开(公告)号：US20140226165A1

公开(公告)日：2014-08-14

申请号：US14258417

申请日：2014-04-22

Applicant: Academia Sinica

Inventor： Chau-Hwang Lee ,  Chun-Chieh Wang

IPC: G01B11/25 ,  G01B11/06

CPC classification number: G01B11/25 ,  G01B11/0608 ,  G06T7/521 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/30004

Abstract: A method for imaging an object using a microscope includes obtaining axial response data, the axial response data representative of a relationship between a separation between a top surface of the object and an objective lens of the microscope and an intensity of light reflected by the top surface of the object; positioning the object at a distance from the objective lens that is within a linear region of the axial response data; sequentially illuminating the object with a plurality of periodic patterns; obtaining a plurality of images of the object, each image resulting from the illumination of the object with a corresponding one of the plurality of periodic patterns; determining a reconstructed image of the object based on the plurality of images of the object; and, based on variations in the intensity of the reconstructed image, determining a topographic profile of the top surface of the object.

Abstract translation: 使用显微镜成像物体的方法包括获得轴向响应数据，轴向响应数据表示物体的顶表面与显微镜的物镜之间的间隔与由顶表面反射的光的强度之间的关系 的对象; 将物体定位在与轴向响应数据的线性区域内的离开物镜的距离处; 以多个周期性图案依次照明物体; 获得所述对象的多个图像，所述每个图像由所述对象的照明产生，所述每个图像具有所述多个周期性图案中的对应的一个; 基于对象的多个图像确定对象的重建图像; 并且基于重建图像的强度的变化，确定对象的顶表面的地形轮廓。

公开(公告)号：US20140220042A1

公开(公告)日：2014-08-07

申请号：US14254453

申请日：2014-04-16

Applicant: Academia Sinica

Inventor： Tse Wen Chang ,  Jiun-Bo Chen ,  Pheidias C. Wu ,  Alfur F. Hung

IPC: C07K16/28

CPC classification number: C07K16/2803 ,  A61K39/39566 ,  C07K16/4291 ,  C07K2317/24 ,  C07K2317/30 ,  C07K2317/34 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  Y10S424/805 ,  Y10S424/81 ,  Y10S530/862 ,  Y10S530/868

Abstract: The invention pertains to the generation and utility of antibodies that can bind effectively to CεmX domain on membrane-bound IgE (mIgE) expressed on the surface of human B lymphocytes. The CεmX domain of 52 amino acid residues, located between the CH4 domain and the C-terminal membrane-anchor peptide on human membrane-bound epsilon chain, had been suggested as an antigenic site for immunological targeting of B cells expressing mIgE. Previous reported monoclonal antibodies, including a20, which bind to RADWPGPP (SEQ ID NO:1) peptide at the C-terminal of CεmX, have now been found to bind poorly to mIgE on human B cells. We have discovered that only monoclonal antibodies specific for certain segments, such as GLAGGSAQSQRAPDRVL (SEQ ID NO:2) and HSGQQQGLPRAAGGSVPHPR (SEQ ID NO:3), of CεmX can bind effectively to mIgE on human B cells and hence have the utility for targeting those B cells for the treatment of diseases mediated by IgE.

Abstract translation: 本发明涉及能够在人B淋巴细胞表面表达的膜结合IgE（mIgE）上有效结合C＆amp; mX结构域的抗体的产生和应用。 已经提出位于人膜结合的ε链上的CH4结构域和C末端膜锚定肽之间的52个氨基酸残基的Cys区域作为用于免疫靶向表达mIgE的B细胞的抗原位点。 目前已经发现，以前报道的，在C细胞C末端结合RADWPGPP（SEQ ID NO：1）肽的单克隆抗体，包括a20，现在已被发现与人B细胞上的mIgE结合不良。 我们已经发现，对于某些片段（例如GLAGGSAQSQRAPDRVL（SEQ ID NO：2）和HSGQQQGLPRAAGGSVPHPR（SEQ ID NO：3））特异性的单克隆抗体，C＆amp; mX可以有效地结合到人B细胞上的mIgE，因此具有效用 用于靶向这些B细胞用于治疗由IgE介导的疾病。

公开(公告)号：US20140178482A1

公开(公告)日：2014-06-26

申请号：US14167243

申请日：2014-01-29

Applicant: Academia Sinica

Inventor： Ting-Fang Wang ,  Shu-Mei Liang

IPC: A61K39/135

CPC classification number: A61K39/135 ,  A61K39/12 ,  A61K2039/5258 ,  A61K2039/552 ,  C07K2319/21 ,  C07K2319/35 ,  C07K2319/95 ,  C12N7/00 ,  C12N2770/32123 ,  C12N2770/32134

Abstract: A method for producing picornaviral capsid protein complexes (e.g., picornavirus like particles) in E. coli using a small-ubiquitin-related fusion protein expression system and an E. coli strain used in practicing this method. Also disclosed is use of the picornaviral capsid protein complexes like thus prepared for eliciting immune responses.

Abstract translation: 使用小泛素相关融合蛋白表达系统和用于实施该方法的大肠杆菌菌株在大肠杆菌中生产小核糖核酸病毒衣壳蛋白复合物（例如，小核糖核酸病毒样颗粒）的方法。 还公开了如此制备的小核糖核酸病毒衣壳蛋白复合物用于引发免疫应答。

公开(公告)号：US08754121B2

公开(公告)日：2014-06-17

申请号：US13213897

申请日：2011-08-19

Applicant: Lie-Fen Shyur ,  Wen-Wan Chao ,  Ya-Wen Cheng

Inventor： Lie-Fen Shyur ,  Wen-Wan Chao ,  Ya-Wen Cheng

IPC: A61K31/34 ,  A61K33/24 ,  A61K33/36 ,  A61K31/19 ,  A61K31/28 ,  A61K31/35 ,  A61K31/44 ,  A61K31/56 ,  A61K31/66 ,  A61K31/69 ,  A61K31/70 ,  A61K31/135 ,  A61K31/175 ,  A61K31/335 ,  A61K31/415 ,  A61K31/445 ,  A61K31/497 ,  A61K31/505

CPC classification number: A61K31/365 ,  A61K31/415 ,  A61K31/4188 ,  A61K31/436 ,  A61K31/44 ,  A61K31/454 ,  A61K31/475 ,  A61K31/506 ,  A61K31/56 ,  A61K31/655 ,  A61K31/69 ,  A61K31/704 ,  A61K33/24 ,  A61K36/28 ,  A61K45/06

Abstract: Methods for treating melanoma in a subject in need thereof are disclosed. The method comprises administering to the subject a composition comprising a therapeutically effective amount of deoxyelephantopin or an analogue thereof; and a pharmaceutically acceptable carrier. Methods of inhibiting proliferation, migration and/or metastasis of melanoma cells in a subject in need thereof are also disclosed. Also disclosed are methods for reducing side effects of an anti-cancer agent in a subject in need thereof.

Abstract translation: 公开了在有需要的受试者中治疗黑素瘤的方法。 该方法包括向受试者施用包含治疗有效量的脱氧耳环素或其类似物的组合物; 和药学上可接受的载体。 还公开了抑制有需要的受试者中黑素瘤细胞增殖，迁移和/或转移的方法。 还公开了减少有需要的受试者中抗癌剂的副作用的方法。

公开(公告)号：US20140141109A1

公开(公告)日：2014-05-22

申请号：US14055545

申请日：2013-10-16

Applicant: ACADEMIA SINICA

Inventor： Pei-Wen Hsiao

IPC: A61K36/28

CPC classification number: A61K36/28

Abstract: Disclosed herein is a method for treating an androgen-stimulated disease in a subject in need thereof by administering to the subject a composition containing an effective amount of an inhibitor of IκB kinase subunit α activity. The composition can also be administered to a subject at high risk for prostate cancer as a method for reducing the subject's risk thereof. A further method relates to identifying a modulator of transcriptional activity regulated by IκB kinase subunit α. Still another method relates to determining the IκB kinase subunit α-regulated transcriptional activity regulated by in a non-human mammalian test subject.

Abstract translation: 本文公开了通过向受试者施用含有有效量的I＆kgr B激酶亚基α活性抑制剂的组合物来治疗有需要的受试者中的雄激素刺激的疾病的方法。 该组合物也可以作为降低受试者风险的方法施用于患有前列腺癌的高风险的受试者。 另一种方法涉及鉴定由I＆kgr; B激酶亚基α调节的转录活性的调节剂。 另一种方法涉及确定由非人哺乳动物测试受试者调节的I激酶亚基α调节的转录活性。

公开(公告)号：US20140131204A1

公开(公告)日：2014-05-15

申请号：US13675735

申请日：2012-11-13

Applicant: ACADEMIA SINICA

Inventor： Chia-Fu Chou ,  Kuo-Tang Liao

IPC: G01N27/447

CPC classification number: B03C5/005 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0896 ,  B01L2400/0424 ,  B01L2400/086 ,  B03C5/026 ,  B03C2201/26 ,  G01N15/06 ,  G01N15/0612 ,  G01N27/44713 ,  G01N2015/0065

Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.

Abstract translation: 公开了用于使用无电极介电电泳（eDEP）快速富集和质量传递生物分子（例如蛋白质）或其它小分子和颗粒的方法，结构，装置和系统。 一方面，用于聚集分子的装置包括电绝缘的基板，形成在基板上并构造成形成用于承载含有颗粒的导电流体的通道的电绝缘材料，由电绝缘材料形成的收缩结构， 位于所述通道中以使通道尺寸变窄并形成尺寸在纳米范围内的开口，以及耦合到所述衬底的电路，沿着所述通道施加交流电场和直流偏置电场，其中所述收缩结构为 结构化以放大施加的交流电场以产生共同操作以聚集颗粒的力。