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    Method for separation and characterization of microorganisms using identifier agents
    33.
    发明申请
    Method for separation and characterization of microorganisms using identifier agents 有权
    使用识别剂分离和表征微生物的方法

    公开(公告)号：US20100129814A1

    公开(公告)日：2010-05-27

    申请号：US12589985

    申请日：2009-10-30

    申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

    发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

    IPC分类号： C12Q1/68 ,  G01N33/567 ,  C12Q1/37 ,  C12Q1/04

    CPC分类号： C12Q1/24 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for the use of one or more identifier agents and interrogating the microorganism sample and/or said one or more identifier agents to produce measurements which characterizing and/or identifying the microorganism based on the produced measurements and/or the presence or absence of the identifier agent or a metabolized form of the identifier agent in the microorganism sample.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供一种或多种识别剂的使用，并且询问微生物样品和/或所述一种或多种标识物以产生基于产生的测量值和/或存在或不存在来表征和/或鉴定微生物的测量结果 的识别剂或微生物样品中识别剂的代谢形式。

    Sealer for test sample devices
    34.
    发明申请
    Sealer for test sample devices 有权

    公开(公告)号：US20080314527A1

    公开(公告)日：2008-12-25

    申请号：US12228782

    申请日：2008-08-14

    申请人： James Clement Bishop ,  Michael James Justin ,  Mark Joseph Fanning

    发明人： James Clement Bishop ,  Michael James Justin ,  Mark Joseph Fanning

    IPC分类号： B32B38/00

    CPC分类号： G01N35/00029 ,  G01N2035/00148 ,  G01N2035/0465 ,  Y10T156/1313 ,  Y10T436/115831 ,  Y10T436/12

    摘要： A sealer for a sample testing instrument is disclosed that cuts and seals a fluid conduit that connects a test sample device with a fluid receptacle containing a fluid sample. The sealer includes an enclosure and a protective shield to protect a user or technician from contact with the cutting element assembly in the sealer. The cutting element assembly includes a spring-loaded element that engages the test sample device and holds it in a fixed position while a cutting element (e.g., hot wire) cuts the fluid conduit. A motor driving the cutting element assembly is positioned at an angle having both vertical and horizontal components, whereby adjustment of the motor firmware allows for adjustment of the position of the cutting element relative to the instrument in both horizontal and vertical directions.

    Method for detecting microorganisms
    36.
    发明授权
    Method for detecting microorganisms 有权
    微生物检测方法

    公开(公告)号：US07183073B2

    公开(公告)日：2007-02-27

    申请号：US10428655

    申请日：2003-05-02

    申请人： Jones M. Hyman ,  Paul M. Matsumura ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe

    发明人： Jones M. Hyman ,  Paul M. Matsumura ,  Scott R. Jeffrey ,  Martin J. Maresch ,  Thurman C. Thorpe

    IPC分类号： C12Q1/02 ,  C12Q1/04 ,  C12N11/02 ,  C12N11/10 ,  C12N11/08 ,  C12M1/34

    CPC分类号： C12M25/14 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/24

    摘要： A device and method are provided for isolating and culturing microorganisms from a bulk fluid sample. The device comprises a container having therein a polymeric immobilization layer having interstitial spaces between polymer chains such as a gel matrix. The interstitial spaces are of an average size less than an average size of microorganisms to be separated from the sample and cultured. A bulk fluid sample is applied to the immobilization layer where fluid is absorbed by the layer and microorganisms remain on the surface of the layer. After culturing, microorganism colonies are readily accessible on the surface of the layer for harvest and testing. The immobilization layer may contain one or more of nutrients for microorganisms growth, lytic agents, lytic enzymes, antibiotics, antibiotic neutralizers, indicators, detergents and selective agents. An adjacent support layer may be above and/or below the immobilization layer. The immobilization layer may be in combination with a sensor layer that changes color in areas corresponding to portions of the layer having microorganisms thereon. A membrane may be embedded in the immobilization layer for enhancing microorganism visibility and facilitating microorganism harvest.

    摘要翻译： 提供了用于从大量流体样品中分离和培养微生物的装置和方法。 该装置包括其中具有聚合物固定化层的容器，其在聚合物链之间具有间隙，例如凝胶基质。 间隙空间的平均尺寸小于从样品中分离的微生物的平均尺寸并进行培养。 将大量流体样品施加到固定层，其中流体被层吸收，微生物保留在层的表面上。 培养后，微生物菌落易于在层的表面进行收获和测试。 固定层可以含有一种或多种用于微生物生长的营养物，溶解剂，溶解酶，抗生素，抗生素中和剂，指示剂，洗涤剂和选择剂。 相邻的支撑层可以在固定层的上方和/或下方。 固定层可以与在其上具有微生物层的部分相对应的区域中改变颜色的传感器层组合。 膜可以嵌入在固定层中以增强微生物的可见性并促进微生物收获。

    Carrier for holding test samples
    37.
    发明申请
    Carrier for holding test samples 有权
    承载测试样品

    公开(公告)号：US20050089444A1

    公开(公告)日：2005-04-28

    申请号：US10695038

    申请日：2003-10-28

    申请人： Michael Justin ,  James Bishop ,  Douglas Hopper ,  Mark Fanning

    发明人： Michael Justin ,  James Bishop ,  Douglas Hopper ,  Mark Fanning

    IPC分类号： B01L9/00 ,  G01N35/00 ,  G01N35/04

    CPC分类号： B01L9/527 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/0465 ,  Y10T436/113332 ,  Y10T436/2575

    摘要： A carrier for holding up to N test sample devices as they are moved through a sample testing instrument. Each of the test sample devices are held in a receiving structure such as a slot in the carrier. The carrier also includes N optical interrupt positioning features, each placed in registry with one of the receiving structures (and thereby in registry with the test sample device). The instrument includes fixed optical interrupt sensors for detecting the position of the positioning feature as the carrier is moved through the instrument. In the illustrated embodiment, the position features comprise voids formed in a rib on the lower surface of the carrier. The optical interrupt sensors are positioned below the path the carrier travels over, whereby as the carrier moves past the sensor the voids, and hence position of the test sample devices, are detected.

    摘要翻译： 用于在通过样品测试仪器移动时最多可容纳N个测试样品装置的载体。 每个测试样本装置保持在诸如载体中的槽的接收结构中。 载波还包括N个光学中断定位功能，每个功能都放置在其中一个接收结构的注册中（从而与测试样本设备注册）。 仪器包括固定光学中断传感器，用于在载体移动通过仪器时检测定位特征的位置。 在所示实施例中，位置特征包括形成在载体的下表面上的肋中的空隙。 光学中断传感器定位在载体行进路径的下方，由此当载体移动通过传感器时，检测到空隙，从而检测测试样品装置的位置。

    Automatic sample testing machine
    38.
    发明授权
    Automatic sample testing machine 有权
    自动样品检测机

    公开(公告)号：US6086824A

    公开(公告)日：2000-07-11

    申请号：US327110

    申请日：1999-06-07

    申请人： Mark Joseph Fanning ,  Jean-Pierre Bernard Gayral ,  Roger James Morris ,  Ron Robinson ,  William Ernest Seaton ,  David B. Shine ,  Paul Springer

    发明人： Mark Joseph Fanning ,  Jean-Pierre Bernard Gayral ,  Roger James Morris ,  Ron Robinson ,  William Ernest Seaton ,  David B. Shine ,  Paul Springer

    IPC分类号： B01L3/00 ,  G01N1/38 ,  G01N35/00 ,  G01N35/10

    CPC分类号： B01L3/50 ,  G01N35/00029 ,  G01N1/38 ,  G01N2035/00089 ,  G01N2035/00138 ,  G01N2035/00148 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： An automatic sample testing machine for testing samples stored in test cards. The machine has a test sample positioning system for moving a tray containing a plurality of test sample cards and fluid receptacles among various stations in the machine. The machine has a diluting station for adding a predetermined quantity of diluent to the receptacles as needed. A pipetting station transfers fluid from one receptacle to another. A vacuum station is provided having a vacuum chamber moveable relative to the tray between upper and lower positions. The chamber cooperates with the tray to make a sealing engagement with the top surface of the tray when it is lowered to the lower position. A vacuum generator supplies vacuum to the chamber. When the vacuum is released from the chamber, the fluid samples are loaded into the cards from the receptacles. The test sample positioning system moves the tray to a cutting and sealing station and then to an incubation station and loads the cards one at a time into a carousel within the incubation station. A test card transport station transports the test cards from the incubation station to an optical reading station, where optical measurements (e.g., transmittance and/or fluorescence optical testing) is conducted on the wells of the card. When the card has been read, it is either moved back to the incubation station for additional incubation and reading or transferred to a card disposal system when the reading is complete.

    摘要翻译： 用于测试存储在测试卡中的样品的自动样品测试机。 该机器具有测试样品定位系统，用于在机器中的各个站之间移动包含多个测试样品卡和流体容器的托盘。 该机器具有用于根据需要向容器添加预定量的稀释剂的稀释站。 移液站将流体从一个容器传送到另一个容器。 设置真空站，其具有可在上位置和下​​位置之间相对于托盘移动的真空室。 当室下降到较低位置时，室与托盘配合以与托盘的顶表面进行密封接合。 真空发生器为腔室提供真空。 当从室释放真空时，将流体样品从容器装载到卡中。 测试样品定位系统将托盘移动到切割和密封站，然后移动到孵化站，并将卡一次装载到孵育站内的转盘中。 测试卡传输站将测试卡从孵化站运送到光学读取站，其中在卡的孔上进行光学测量（例如，透射率和/或荧光光学测试）。 读卡完成后，将其移回孵化站进行额外的孵化和阅读，或者将其转移到卡片处理系统。

    Vacuum loading of test sample cards in an analytical instrument
    39.
    发明授权
    Vacuum loading of test sample cards in an analytical instrument 失效
    测试样品卡在分析仪器中的真空加载

    公开(公告)号：US5965090A

    公开(公告)日：1999-10-12

    申请号：US6623

    申请日：1998-01-13

    申请人： Mark Joseph Fanning ,  Clifford W. Karl ,  Donald Meyer ,  William Ernest Seaton ,  Paul Springer ,  Craig Drager

    发明人： Mark Joseph Fanning ,  Clifford W. Karl ,  Donald Meyer ,  William Ernest Seaton ,  Paul Springer ,  Craig Drager

    IPC分类号： B01L3/00 ,  G01N1/38 ,  G01N35/00 ,  G01N35/10

    CPC分类号： B01L3/50 ,  G01N35/00029 ,  G01N1/38 ,  G01N2035/00089 ,  G01N2035/00138 ,  G01N2035/00148 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/114165 ,  Y10T436/114998 ,  Y10T436/115831 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： An automatic sample testing machine for testing samples stored in test cards. The machine has a test sample positioning system for moving a tray containing a plurality of test sample cards and fluid receptacles among various stations in the machine. The machine has a diluting station for adding a predetermined quantity of diluent to the receptacles as needed. A pipetting station transfers fluid from one receptacle to another. A vacuum station is provided having a vacuum chamber moveable relative to the tray between upper and lower positions. The chamber cooperates with the tray to make a sealing engagement with the top surface of the tray when it is lowered to the lower position. A vacuum generator supplies vacuum to the chamber. When the vacuum is released from the chamber, the fluid samples are loaded into the cards from the receptacles. The test sample positioning system moves the tray to a cutting and sealing station and then to an incubation station and loads the cards one at a time into a carousel within the incubation station. A test card transport station transports the test cards from the incubation station to an optical reading station, where optical measurements (e.g., transmittance and/or fluorescence optical testing) is conducted on the wells of the card. When the card has been read, it is either moved back to the incubation station for additional incubation and reading or transferred to a card disposal system when the reading is complete.

    摘要翻译： 用于测试存储在测试卡中的样品的自动样品测试机。 该机器具有测试样品定位系统，用于在机器中的各个站之间移动包含多个测试样品卡和流体容器的托盘。 该机器具有用于根据需要向容器添加预定量的稀释剂的稀释站。 移液站将流体从一个容器传送到另一个容器。 设置真空站，其具有可在上位置和下​​位置之间相对于托盘移动的真空室。 当室下降到较低位置时，室与托盘配合以与托盘的顶表面进行密封接合。 真空发生器为腔室提供真空。 当从室释放真空时，将流体样品从容器装载到卡中。 测试样品定位系统将托盘移动到切割和密封站，然后移动到孵化站，并将卡一次装载到孵育站内的转盘中。 测试卡传输站将测试卡从孵化站运送到光学读取站，其中在卡的孔上进行光学测量（例如，透射率和/或荧光光学测试）。 读卡完成后，将其移回孵化站进行额外的孵化和阅读，或者将其转移到卡片处理系统。