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公开(公告)号：US09469872B2
公开(公告)日：2016-10-18
申请号：US14299269
申请日：2014-06-09
CPC分类号： C12P19/34 , C09K3/00 , C12Q1/686 , C12Q2565/518 , C12Q2527/137
摘要： Methods for amplifying nucleic acids are provided. The methods can be used to minimize sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimizing GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilizing flow cells.
摘要翻译： 提供了扩增核酸的方法。 这些方法可以用于最小化由某些核酸序列的优先扩增引起的序列特异性偏差。 描述了可以降低AT富含模板相对于GC富集模板的效率的方法，由此使具有不同序列的多个模板的扩增反应期间的GC偏差最小化。 该方法适用于固相扩增，例如利用流动池。
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公开(公告)号：US09416411B2
公开(公告)日：2016-08-16
申请号：US14157323
申请日：2014-01-16
申请人： Illumina, Inc.
发明人： John R. Stuelpnagel , Mark Chee
IPC分类号： C12Q1/68 , G11C7/10 , H03K19/096
CPC分类号： C12N15/1093 , C12Q1/6806 , C12Q1/6834 , C12Q1/6837 , C12Q1/686 , C12Q1/6876 , G11C7/1048 , H03K19/0966 , Y10S977/792 , C12Q2525/197 , C12Q2523/107
摘要： The present invention is directed to methods and compositions for generating a pool of oligonucleotides. The invention finds use in preparing a population or subpopulations of oligonucleotides in solution. The pool of oligonucleotides finds use in a variety of nucleic acid detection and/or amplification assays.
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33.发明授权公开(公告)号：US09328378B2
公开(公告)日：2016-05-03
申请号：US12309772
申请日：2007-07-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6874 , C12Q2533/107 , C12Q2525/101
摘要： The invention relates to a method of preparing a library of template polynucleotides which reduces and/or prevents the formation of adaptor-dimers. The invention also relates to the use of a library of templates prepared using the method of the invention for solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends which is substantially free of adaptor-dimers.
摘要翻译： 本发明涉及一种制备模板多核苷酸文库的方法，其减少和/或防止适配剂二聚体的形成。 本发明还涉及使用本发明的方法制备的模板文库用于固相核酸扩增的用途。 特别地，本发明涉及一种制备模板多核苷酸文库的方法，该文库在其5'末端和3'末端具有基本上不含适配剂二聚体的共同序列。
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公开(公告)号：US09273352B2
公开(公告)日：2016-03-01
申请号：US14137434
申请日：2013-12-20
发明人： Geoffrey Paul Smith , Roberto Rigatti , Tobias William Barr Ost , Shankar Balasubramanian , Raquel Maria Sanches-Kuiper
CPC分类号： C12N9/1252 , C12Q1/686 , C12Y207/07007
摘要： Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
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公开(公告)号：US09238671B2
公开(公告)日：2016-01-19
申请号：US13979346
申请日：2012-01-30
申请人： Igor Goryshin , Bradley Baas , Ramesh Vaidyanathan , Mark Maffitt
发明人： Igor Goryshin , Bradley Baas , Ramesh Vaidyanathan , Mark Maffitt
CPC分类号： C12N15/1065 , C07H21/00 , C12N9/1241 , C12N15/1093 , C12P19/34 , C12Q1/6855 , C40B50/06
摘要： Transposomes and oligonucleotide replacement methods to make DNA libraries that have distinct 5′ and 3′ tags, and to make directional libraries that are enriched for a desired strand.
摘要翻译： 用于制备具有不同5'和3'标签的DNA文库的转座子和寡核苷酸替代方法，并制备富集所需链的定向文库。
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公开(公告)号：US09222131B2
公开(公告)日：2015-12-29
申请号：US14268690
申请日：2014-05-02
IPC分类号： C07D311/78 , C12Q1/68 , C07D405/14 , C07D311/82 , C07D405/12 , C07D487/04 , C07D491/147
CPC分类号： C12Q1/6869 , C07D311/82 , C07D405/12 , C07D405/14 , C07D487/04 , C07D491/147
摘要： The present invention relates to new rhodamine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.
摘要翻译： 本发明涉及新的罗丹明化合物及其作为荧光标记物的用途。 该化合物可用作核酸测序应用中核苷酸的荧光标记。
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公开(公告)号：US09193999B2
公开(公告)日：2015-11-24
申请号：US14314864
申请日：2014-06-25
申请人： ILLUMINA, INC.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C07H21/02 , C07H21/04 , C12N9/00 , C12P19/30 , C12Q1/6874 , C12Q2521/101 , C12Q2521/119 , C12Q2525/101 , C12Q2533/101 , C12Q2533/107 , C12Q2535/122 , C12Q2565/507 , C12Q2565/518 , C12Q2565/537
摘要： A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.
摘要翻译： 一种用于测序的方法包括以下步骤：（a）提供第一和第二核酸模板，其中两个模板具有不同的序列; （b）使用第一聚合酶种类和第一组核苷酸类似物扩展与第一模板结合的第一引物; （c）使用第二聚合酶种类和第二组核苷酸类似物扩展与第二模板结合的第二引物，其中第一聚合酶种类与第二聚合酶种类不同，并且其中第一组核苷酸类似物不同于第二种 一套核苷酸类似物，（d）检测第一和第二引物延伸产物; 和（e）重复步骤（b）至（d），从而确定第一和第二模板的不同序列。
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公开(公告)号：US09181583B2
公开(公告)日：2015-11-10
申请号：US13790112
申请日：2013-03-08
申请人： Illumina, Inc.
发明人： Thomas Royce , Craig April , Fiona Kaper , Jian-Bing Fan
CPC分类号： G06F19/22 , C12Q1/6827 , C12Q1/6853 , C12Q1/6874 , C12Q1/6881 , C12Q1/6883 , C12Q2600/172 , G06F19/16 , C12Q2525/155 , C12Q2525/161 , C12Q2535/122
摘要： Presented herein are methods and compositions for determining haplotypes in a sample. The methods are useful for obtaining sequence information regarding, for example, HLA type and haplotype. Also presented herein are methods of determining haplotypes in a sample based on a plurality sequence reads.
摘要翻译： 本文提出了用于确定样品中单倍型的方法和组合物。 该方法对于获得关于例如HLA类型和单体型的序列信息是有用的。 本文还呈现了基于多个序列读取来确定样品中的单倍型的方法。
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公开(公告)号：US09169513B2
公开(公告)日：2015-10-27
申请号：US14512693
申请日：2014-10-13
申请人： ILLUMINA, INC.
发明人： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC分类号： C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
摘要： A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
摘要翻译： 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。
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公开(公告)号：US09017945B2
公开(公告)日：2015-04-28
申请号：US13556053
申请日：2012-07-23
申请人： Geoffrey Paul Smith
发明人： Geoffrey Paul Smith
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12Q2535/119
摘要： The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which permit the sequential determination of nucleotide sequences in two distinct and separate regions on complementary strands of the double-stranded polynucleotide template. The two regions for sequence determination may or may not be complementary to each other.
摘要翻译： 本发明涉及用于双链多核苷酸模板的成对测序的方法，其允许在双链多核苷酸模板的互补链上的两个不同和分离的区域中顺序测定核苷酸序列。 用于序列确定的两个区域可以彼此或可以不互补。
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