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    Dynamic Array Assay Methods
    35.
    发明申请
    Dynamic Array Assay Methods 审中-公开
    动态数组测定方法

    公开(公告)号：US20160153026A1

    公开(公告)日：2016-06-02

    申请号：US14880112

    申请日：2015-10-09

    Applicant: FLUIDIGM CORPORATION

    Inventor： Kenneth J. Livak ,  Marc Unger

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6834 ,  C12Q2565/629

    Abstract: High throughput methods are used that combine the features of using a matrix-type microfluidic device, labeled nucleic acid probes, and homogenous assays to detect and/or quantify nucleic acid analytes. The high throughput methods are capable of detecting nucleic acid analyses with high PCR and probe specificity, producing a low fluorescence background and therefore, a high signal to noise ratio. Additionally, the high throughput methods are capable of detecting low copy number nucleic acid analyte per cell.

    Abstract translation: 使用高通量方法，其结合使用基质型微流体装置，标记的核酸探针和均质测定的特征来检测和/或定量核酸分析物。 高通量方法能够以高PCR和探针特异性检测核酸分析，产生低荧光背景，因此具有高的信噪比。 此外，高通量方法能够检测每个细胞的低拷贝数核酸分析物。

    SYSTEM FOR THERMAL CYCLING OF MICROFLUIDIC SAMPLES
    36.
    发明申请
    SYSTEM FOR THERMAL CYCLING OF MICROFLUIDIC SAMPLES 审中-公开
    微流体样品热循环系统

    公开(公告)号：US20160114327A1

    公开(公告)日：2016-04-28

    申请号：US14864491

    申请日：2015-09-24

    Applicant: Fluidigm Corporation

    Inventor： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

    IPC: B01L7/00

    CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12P19/34 ,  C12Q3/00 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/76

    Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.

    Abstract translation: 用于微流体装置的热循环仪包括控制器，其可操作以提供与散热器热连通的一系列电信号，散热器和加热元件，并可操作以从控制器接收一系列电信号。 热循环仪还包括与加热元件热连通的热卡盘。 热卡盘包括可操作以与微流体装置进行热接触的加热表面。 加热表面的特征在于每秒2.5摄氏度和5.5摄氏度/秒之间的温度升高速率，并且支撑微流体装置的第一部分的加热表面的第一部分与加热表面的第二部分之间的温度差 支撑微流体装置的第二部分小于0.25℃

    METHODS FOR DETECTION AND QUANTIFICATION OF NUCLEIC ACID OR PROTEIN TARGETS IN A SAMPLE
    37.
    发明申请
    METHODS FOR DETECTION AND QUANTIFICATION OF NUCLEIC ACID OR PROTEIN TARGETS IN A SAMPLE 有权
    在样品中检测和定量核酸或蛋白质目标的方法

    公开(公告)号：US20160108460A1

    公开(公告)日：2016-04-21

    申请号：US14810388

    申请日：2015-07-27

    Applicant: Fluidigm Corporation

    Inventor： Michael Lucero ,  Marc Unger

    IPC: C12Q1/68 ,  G01N33/68

    CPC classification number: C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6813 ,  C12Q1/6876 ,  C12Q2600/118 ,  G01N33/53 ,  G01N33/6845 ,  G01N2333/435 ,  G01N2458/10

    Abstract: The invention provides an assay method for detection and/or quantification of a plurality of nucleic acid or protein targets in a sample. In the method probes are used to associate a detectable tag sequence with each of the selected targets present in the sample. Probes or primers sufficient to identify at least 25, and preferably at least 500, different targets are used. The method involves segregating aliquots of the sample from each other and detecting the tag sequences in each aliquot.

    Abstract translation: 本发明提供了用于检测和/或定量样品中多个核酸或蛋白质靶标的测定方法。 在该方法中，探针用于将可检测标签序列与存在于样品中的每个选定靶标相关联。 使用足以鉴定至少25个，优选至少500个不同靶标的探针或引物。 该方法包括将样品的等分试样彼此分离并检测每个等分试样中的标签序列。

    Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
    38.
    发明授权
    Methods, systems and devices for multiple single-cell capturing and processing using microfluidics 有权
    使用微流体法进行多单细胞捕获和处理的方法，系统和装置

    公开(公告)号：US09304065B2

    公开(公告)日：2016-04-05

    申请号：US13781307

    申请日：2013-02-28

    Applicant: Fluidigm Corporation

    Inventor： Brian Fowler ,  Jake Kimball ,  Myo Thu Maung ,  Andrew May ,  Michael C. Norris ,  Dominique G. Toppani ,  Marc A. Unger ,  Jing Wang ,  Jason A. A. West

    IPC: C12Q1/68 ,  G01N1/28 ,  C12P19/34 ,  G01N1/34 ,  G01N15/14 ,  B01L3/00 ,  B01L7/00

    CPC classification number: G01N1/28 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0864 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L2400/088 ,  C12P19/34 ,  C12Q1/6813 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N1/34 ,  G01N15/1484 ,  C12Q2565/629

    Abstract: Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases.

    Abstract translation: 描述了利用微流体的多单细胞捕获和处理的方法，系统和装置。 提供的工具和技术用于捕获，分配和/或操纵来自较大群体的细胞的单个细胞以及产生与每个单个细胞相关的遗传信息和/或反应。 可以使用不同的捕获配置来捕获单个细胞，然后在多室反应配置中处理每个单独的细胞。 一些实施方案可以提供已经从较大群体中划分的多个单个细胞的特异性靶扩增，全基因组扩增，全转录组扩增，实时PCR制备，拷贝数变异，预扩增，mRNA测序和/或单倍型 细胞。 一些实施例可以提供其他应用。 一些实施例可以被配置为用于对作为处理的一部分的各个单元或相关联的反应产物进行成像。 在某些情况下可以收获和/或进一步分析反应产物。

    METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS
    39.
    发明申请
    METHODS AND DEVICES FOR ANALYSIS OF DEFINED MULTICELLULAR COMBINATIONS 审中-公开
    用于分析定义的多细胞组合的方法和装置

    公开(公告)号：US20160025761A1

    公开(公告)日：2016-01-28

    申请号：US14775685

    申请日：2014-03-14

    Applicant: FLUIDIGM CORPORATION

    Inventor： Jason A. A. West ,  Brian Fowler

    IPC: G01N35/08 ,  B01L3/00 ,  C12M1/04 ,  C12M1/00 ,  G01N33/50 ,  G01N33/569

    Abstract: Methods for cell analysis are provided, comprising cell capturing, characterization, transport, and culture. In an exemplary method individual cells (and/or cellular units) are flowed into a microfluidic channel, the channel is partitioned into a plurality of contiguous segments, capturing at least one cell in at least one segment, A characteristic of one or more captured cells is determined and the cell(s) and combinations of cells are transported to specified cell holding chamber(s) based on the determined characteristic(s). Also provided are devices and systems for cell analysis.

    Abstract translation: 提供了用于细胞分析的方法，包括细胞捕获，表征，转运和培养。 在一个示例性方法中，个体细胞（和/或细胞单位）流入微流体通道，通道被划分成多个连续区段，捕获至少一个区段中的至少一个细胞，一个或多个捕获的细胞的特征 并且基于所确定的特性将单元和单元的组合输送到指定的单元容纳室。 还提供了用于细胞分析的装置和系统。

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