公开(公告)号：US20150053562A1

公开(公告)日：2015-02-26

申请号：US14526928

申请日：2014-10-29

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0427 ,  C12Q1/6806 ,  G01N15/1031 ,  G01N27/44717 ,  G01N27/44721 ,  G01N33/543 ,  G01N35/10 ,  G01N2015/0065 ,  G01N2015/0088 ,  G01N2015/1081 ,  G01N2035/1034 ,  Y10T436/11 ,  Y10T436/118339 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The present invention relates to droplet-based particle sorting. According to one embodiment, a droplet microactuator is provided and includes: (a) a suspension of particles; and (b) electrodes arranged for conducting droplet operations using droplets comprising particles. A method of transporting a particle is also provided, wherein the method includes providing a droplet comprising the particle and transporting the droplet on a droplet microactuator.

Abstract translation: 本发明涉及基于液滴的颗粒分选。 根据一个实施例，提供了液滴微致动器，其包括：（a）颗粒悬浮液; 和（b）布置成使用包含颗粒的液滴进行液滴操作的电极。 还提供了输送颗粒的方法，其中所述方法包括提供包含颗粒的液滴并将液滴输送到液滴微致动器上。

公开(公告)号：US20140302580A1

公开(公告)日：2014-10-09

申请号：US14308110

申请日：2014-06-18

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen Eckhardt

IPC: G01N1/38

CPC classification number: G01N33/54326 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0809 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N1/38 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention provides a method of dispersing or circulating magnetically responsive beads within a droplet in a droplet actuator. The invention, in one embodiment, makes use of a droplet actuator with a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at a portion of the plurality of droplet operations electrodes. A bead-containing droplet is provided on the droplet actuator in the presence of the uniform magnetic field. Beads are circulated in the droplet during incubation by conducting droplet operations on the droplet within a uniform region of the magnate field. Other embodiments are also provided.

Abstract translation: 本发明提供了一种在液滴致动器中的液滴内分散或循环磁响应珠的方法。 在一个实施例中，本发明利用具有配置成传送液滴的多个液滴操作电极的液滴致动器和存在于多个液滴操作电极的一部分处的磁场。 在均匀磁场存在的情况下，在液滴致动器上提供含珠的液滴。 在孵育期间，通过在大气场的均匀区域内的液滴上进行液滴操作，珠子在液滴中循环。 还提供了其他实施例。

公开(公告)号：US20140231259A1

公开(公告)日：2014-08-21

申请号：US14253556

申请日：2014-04-15

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula

IPC: G01N27/447

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator including an opaque filler fluid and a transparent droplet thereon is provided. The droplet microactuator may further include a first substrate comprising electrodes configured to effect electrowetting mediated droplet operations on a surface of the first substrate; a second substrate spaced apart from the surface of the first substrate by a distance sufficient to define an interior volume between the first substrate and second substrate; and wherein the droplet and the filler fluid are in the interior volume, and wherein the droplet is surrounded by the filler fluid and arranged with respect to the electrodes in a manner which permits electrowetting mediated droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了包括不透明填充流体和其上的透明液滴的液滴微致动器。 液滴微致动器还可以包括第一衬底，其包括被配置为在第一衬底的表面上实现电润湿介导的液滴操作的电极; 第二基板，与第一基板的表面间隔开足以限定第一基板和第二基板之间的内部空间的距离; 并且其中所述液滴和所述填充流体在所述内部容积中，并且其中所述液滴被所述填充流体包围并且以允许使用所述电极对所述液滴进行电润湿介导的液滴操作的方式相对于所述电极布置。

公开(公告)号：US12181467B2

公开(公告)日：2024-12-31

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/12 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US11789015B2

公开(公告)日：2023-10-17

申请号：US16948074

申请日：2020-09-01

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt

IPC: G01N33/543 ,  B01L3/00 ,  B01F31/65 ,  B01F33/451 ,  B01F33/302 ,  B01F33/3031 ,  G01N1/38 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54326 ,  B01F31/65 ,  B01F33/3021 ,  B01F33/3031 ,  B01F33/451 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502792 ,  G01N1/38 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/089 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0436 ,  B01L2400/0442 ,  G01N2001/386 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575 ,  Y10T436/25625

Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

公开(公告)号：US11255809B2

公开(公告)日：2022-02-22

申请号：US16788187

申请日：2020-02-11

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  B01F13/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  G01N27/416 ,  B01L3/02

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US10809254B2

公开(公告)日：2020-10-20

申请号：US16191270

申请日：2018-11-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Ramakrishna Sista ,  Vijay Srinivasan ,  Michael G. Pollack ,  Allen E. Eckhardt

IPC: G01N1/40 ,  G01N1/00 ,  G01N1/10 ,  G01N1/22 ,  G01N1/18 ,  G01N35/00 ,  G01N1/38 ,  B01L3/00 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01F13/08 ,  B01L7/00 ,  G01N35/10

Abstract: Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.

公开(公告)号：US10379112B2

公开(公告)日：2019-08-13

申请号：US14719731

申请日：2015-05-22

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447 ,  G01N33/543 ,  C12M1/12 ,  G01N35/10 ,  G01N35/00

Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

公开(公告)号：US10005080B2

公开(公告)日：2018-06-26

申请号：US14538506

申请日：2014-11-11

Applicant: GENMARK DIAGNOSTICS, INC. ,  ADVANCED LIQUID LOGIC, INC.

Inventor： Jon Faiz Kayyem ,  Sean Ford ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Scott Corey ,  Brian Murphy ,  David Walter Wright ,  Dominic Aiello

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502 ,  B01L3/502792 ,  B01L3/52 ,  B01L7/525 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/04 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0874 ,  B01L2300/123 ,  B01L2300/1822 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0683

Abstract: In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.

公开(公告)号：US09952177B2

公开(公告)日：2018-04-24

申请号：US14795304

申请日：2015-07-09

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Michael G. Pollack ,  Vijay Srinivasan ,  Zhishan Hua ,  Hon Lung Chu ,  Michael Hauser ,  Allen Eckhardt

IPC: G01N27/447 ,  G01N27/453 ,  B01L3/00 ,  B01L7/00

CPC classification number: G01N27/44743 ,  B01L3/502715 ,  B01L3/502792 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/0457 ,  B01L2400/046 ,  G01N27/44791

Abstract: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.