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公开(公告)号：US06270644B1
公开(公告)日：2001-08-07
申请号：US09238131
申请日：1999-01-27
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： A Monster Capillary Array Electrophoresis scanner measures four-color electropherograms from over a thousand capillary electrophoretic separations in parallel. The system consists of a two-dimensional confocal rotary scanner and a four-color detection unit.
摘要翻译： 怪物毛细管阵列电泳扫描仪测量并行从一千个毛细管电泳分离的四色电泳图。 该系统由二维共焦旋转扫描仪和四色检测单元组成。
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公开(公告)号：US6143152A
公开(公告)日：2000-11-07
申请号：US965738
申请日：1997-11-07
IPC分类号： C08F2/58 , G01N27/447 , G01N37/00 , G01N27/26
CPC分类号： G01N27/44791 , G01N27/44782 , Y10S366/03
摘要： A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.
摘要翻译： 毛细管阵列电泳（CAE）微板，其具有连接到板上的样品储存器阵列的分离通道阵列。 样品池被组织成一个或多个样品注射器。 提供一个或多个废物储存器以从每个样品注射器中的储存器收集废物。 此外，阴极储存器也与一个或多个分离通道复用。 为了完成电路径，在微板上还设置有一些或所有分离通道共用的阳极储存器。 此外，通道布局保持从阳极到每个阴极的距离近似恒定。
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公开(公告)号：US5688648A
公开(公告)日：1997-11-18
申请号：US574890
申请日：1995-12-19
申请人： Richard A. Mathies , Alexander Glazer , Jingyue Ju
发明人： Richard A. Mathies , Alexander Glazer , Jingyue Ju
IPC分类号： G01N33/53 , C12Q1/68 , G01N33/533 , G01N33/542 , G01N33/566 , G01N33/58
CPC分类号： G01N33/582 , G01N33/542 , Y10S435/81
摘要： Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
摘要翻译： 提供的组合物包括携带供体和受体染料分子对的一组荧光标记，设计用于以单一波长有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变在给定对中的供体和受体之间的距离而在分离条件下具有基本上相同的迁移率。 特别地，荧光组合物在测序核酸中可用作标记。
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公开(公告)号：US5654419A
公开(公告)日：1997-08-05
申请号：US189924
申请日：1994-02-01
申请人： Richard A. Mathies , Alexander Glazer , Jingyue Ju
发明人： Richard A. Mathies , Alexander Glazer , Jingyue Ju
IPC分类号： G01N33/53 , C12Q1/68 , G01N33/533 , G01N33/542 , G01N33/566 , G01N33/58 , C07H21/00
CPC分类号： G01N33/582 , G01N33/542 , Y10S435/81
摘要： Compositions are provided comprising sets of fluorescent labels carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donors at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.
摘要翻译： 提供的组合物包括携带供体和受体染料分子对的一组荧光标记，设计用于以单一波长有效激发供体，并以不同波长的每对中的受体发射。 具有不同供体 - 受体对的不同分子可以通过改变在给定对中的供体和受体之间的距离而在分离条件下具有基本上相同的迁移率。 特别地，荧光组合物在测序核酸中可用作标记。
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公开(公告)号：US09835624B2
公开(公告)日：2017-12-05
申请号：US14111677
申请日：2012-04-16
IPC分类号： A61K39/04 , A61K39/395 , A61K39/40 , G01N33/569 , G01N33/68
CPC分类号： G01N33/5695 , G01N33/6854 , G01N2469/20 , G01N2800/52
摘要： The present disclosure provides methods of detecting mycobacterium in an individual, generally involving detecting antibody to a mycobacterial lipid in a biological sample obtained from the individual. The present disclosure further provides compositions and kits for carrying out the methods.
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36.发明授权Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions 有权用于工程化单分散乳液中单分子/细胞遗传分析的微型液滴发生器公开(公告)号：US08454906B2
公开(公告)日：2013-06-04
申请号：US12670377
申请日：2008-07-24
CPC分类号： B01L3/0268 , B01F3/0807 , B01F13/0062 , B01F13/1013 , B01F13/1022 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00722 , B01L2200/0673 , B01L2300/0803 , B01L2400/0439 , B01L2400/0481 , G01N35/08 , G01N2035/1034 , Y10T436/2575
摘要： Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.
摘要翻译： 提供了用于在乳液油中快速产生试剂/靶（例如分子或细胞）混合物的单分散纳升体积液滴的微流体设计和方法。 这些设计和方法使得能够在受控尺寸的试剂混合液滴中的单个靶（例如，DNA / RNA分子或细胞）的高通量包封。 根据各种实施例，使用微型三阀泵来精确地测量每个液滴中的试剂/目标混合物的体积，并且还有效地将诸如珠粒和细胞的微粒有效地引入到装置中，该装置被封装在相交处的液滴内 的试剂通道和油通道。 微型泵的脉动流动分布提供了对液滴产生的主动控制，从而使得能够与与生物反应相容的油形成液滴，但是否则难以与乳液形成乳液。
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37.发明申请MICROFABRICATED DROPLET GENERATOR FOR SINGLE MOLECULE/CELL GENETIC ANALYSIS IN ENGINEERED MONODISPERSED EMULSIONS 有权用于单分子乳液中单分子/细胞遗传分析的微生物滴定发生器公开(公告)号：US20100285975A1
公开(公告)日：2010-11-11
申请号：US12670377
申请日：2008-07-24
CPC分类号： B01L3/0268 , B01F3/0807 , B01F13/0062 , B01F13/1013 , B01F13/1022 , B01J2219/00466 , B01J2219/00468 , B01J2219/005 , B01J2219/00596 , B01J2219/00722 , B01L2200/0673 , B01L2300/0803 , B01L2400/0439 , B01L2400/0481 , G01N35/08 , G01N2035/1034 , Y10T436/2575
摘要： Provided are microfluidic designs and methods for rapid generation of monodisperse nanoliter volume droplets of reagent/target (e.g., molecule or cell) mix in emulsion oil. The designs and methods enable high-throughput encapsulation of a single target (e.g., DNA/RNA molecules or cells) in controlled size droplets of reagent mix. According to various embodiments, a microfabricated, 3-valve pump is used to precisely meter the volume of reagent/target mix in each droplet and also to effectively route microparticles such as beads and cells into the device, which are encapsulated within droplets at the intersection of the reagent channel and an oil channel. The pulsatile flow profile of the microfabricated pumps provides active control over droplet generation, thereby enabling droplet formation with oils that are compatible with biological reactions but are otherwise difficult to form emulsions with.
摘要翻译： 提供了用于在乳液油中快速产生试剂/靶（例如分子或细胞）混合物的单分散纳升体积液滴的微流体设计和方法。 这些设计和方法使得能够在受控尺寸的试剂混合液滴中的单个靶（例如，DNA / RNA分子或细胞）的高通量包封。 根据各种实施例，使用微型三阀泵来精确地测量每个液滴中的试剂/目标混合物的体积，并且还有效地将诸如珠粒和细胞的微粒有效地引入装置中，该装置被封装在相交处的液滴内 的试剂通道和油通道。 微型泵的脉动流动分布提供了对液滴产生的主动控制，从而使得能够与与生物反应相容的油形成液滴，但是否则难以与乳液形成乳液。
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公开(公告)号：US07445926B2
公开(公告)日：2008-11-04
申请号：US10750533
申请日：2003-12-29
CPC分类号： F04B43/043 , B01F5/10 , B01F11/0071 , B01F13/0059 , B01L3/5025 , B01L3/502707 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/10 , B01L2300/0654 , B01L2300/0803 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/087 , B01L2300/088 , B01L2300/0887 , B01L2300/1827 , B01L2400/0409 , B01L2400/0421 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , C12Q1/6806 , C12Q1/689 , C12Q2565/629 , F04B43/06 , F16K99/0001 , G01N35/00069 , Y10T137/0318 , Y10T137/2224 , Y10T137/87716
摘要： Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.
摘要翻译： 提供了实现微流体分析装置的方法和装置。 与集成的气动歧管相关联的整体弹性体膜片允许放置和致动各种流体控制结构，例如用于泵送，隔离，混合，路由，合并，分离，制备和存储流体体积的结构。 流体控制结构可用于实施各种样品引入，制备，加工和储存技术。
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39.发明授权Apparatus and methods for high resolution separation of sample components on microfabricated channel devices 有权用于在微细化通道器件上高分辨率分离样品组分的装置和方法公开(公告)号：US06913679B1
公开(公告)日：2005-07-05
申请号：US09249274
申请日：1999-02-11
IPC分类号： G01N21/64 , C12M1/00 , C12N15/09 , G01N27/447 , G01N37/00 , B01L3/02 , G01N27/453 , G01N30/02
CPC分类号： G01N27/44791
摘要： Sample component separation apparatus and methods are described. An exemplary sample component separation apparatus includes a separation channel having a turn portion configured to reduce band-broadening caused by passage of a sample through the turn portion. To reduce band broadening caused by passage of a sample through a turn portion, the turn portion may be constructed and arranged to have a sample transport characteristic that is different from the corresponding sample transport characteristic of a substantially straight portion of the separation channel. For example, the turn portion may be configured with an effective channel width that is smaller than the effective channel widths of the substantially straight portion of the separation channel. The actual channel width of the turn portion may be smaller than the channel widths of the substantially straight portion; the effective channel width of the turn portion may be reduced by placing one or more sample transport barriers or constrictions in the turn portion of the channel. Alternatively, the sample velocity through the turn portion may be controlled so as to reduce band broadening. For example, sample transport barriers may be disposed in the turn portion so that sample components of a given band travel through the turn portion at substantially the same effective rate, whereby the band orientation remains substantially aligned along radial directions characteristic of the turn portion. Other a sample transport characteristics, such as electrical resistance or fluid flow resistance, of the turn portion may be adapted to reduce band broadening caused by passage of the sample through the turn portion.
摘要翻译： 描述了样品组分分离装置和方法。 示例性部件分离装置包括分离通道，该分离通道具有弯曲部分，其被配置为减少由于样品通过转弯部分而导致的带宽变形。 为了减少由于样品通过转弯部分而导致的带宽增加，转弯部分可以被构造和布置成具有不同于分离通道的基本直的部分的相应样品输送特性的样品输送特性。 例如，转弯部分可以被配置为具有小于分离通道的基本上直的部分的有效通道宽度的有效通道宽度。 转弯部分的实际通道宽度可以小于基本上直的部分的通道宽度; 可以通过在通道的转弯部分放置一个或多个样品输送屏障或收缩部来减小转弯部分的有效通道宽度。 或者，可以控制通过转弯部分的采样速度，以便减小频带变宽。 例如，样品输送屏障可以设置在转弯部分中，使得给定带的样品成分以基本上相同的有效速率行进通过转弯部分，由此带状取向保持基本上与转向部分的径向方向对准。 转向部分的其它样品输送特性，例如电阻或流体阻力可以适于减少由于样品通过转弯部分而导致的带宽变宽。
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公开(公告)号：US06554986B1
公开(公告)日：2003-04-29
申请号：US09579076
申请日：2000-05-25
IPC分类号： G01N2726
CPC分类号： G01N27/44721 , G01N27/44782
摘要： An improved rotary confocal fluorescence scanner capable of detecting analytes separated on over a 1,000 capillaries simultaneously. This system uses a confocal microscope objective and mirror assembly that rotates inside a vertical ring of capillaries to provide rapid and efficient excitation and detection of fluorescently labeled fragments separated within a cylindrical capillary array. Use of automated procedures to load and run all capillaries permits one to read more than 350,000 base pairs of raw sequence data per hour.
摘要翻译： 改进的旋转共聚焦荧光扫描仪能够同时检测超过1,000个毛细管分离的分析物。 该系统使用在垂直的毛细管环内旋转的共焦显微镜物镜和镜组件，以提供在圆柱形毛细管阵列内分离的荧光标记片段的快速和有效的激发和检测。 使用自动化程序加载和运行所有毛细管，可以读取每小时超过35万个碱基对原始序列数据。
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