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    Antibody fragments specific for human carcinoembryonic antigen (CEA)
    33.
    发明申请
    Antibody fragments specific for human carcinoembryonic antigen (CEA) 审中-公开
    针对人癌胚抗原（CEA）特异的抗体片段

    公开(公告)号：US20070199078A1

    公开(公告)日：2007-08-23

    申请号：US11731442

    申请日：2007-03-30

    申请人： Jorge Cowley ,  Marta Avila ,  Freya Freyre Almeida ,  Boris Castro ,  Hanssel Garcia ,  Lourdes Navarro ,  Luis Lopez ,  Jose Alvarez ,  Raquel Segui

    发明人： Jorge Cowley ,  Marta Avila ,  Freya Freyre Almeida ,  Boris Castro ,  Hanssel Garcia ,  Lourdes Navarro ,  Luis Lopez ,  Jose Alvarez ,  Raquel Segui

    IPC分类号： A61K51/00 ,  C12N5/06 ,  A61K39/395

    CPC分类号： C07K14/22 ,  C07K16/3007 ,  C07K2317/622 ,  C07K2317/626

    摘要： The invention refers to mono- and divalent (diabody) single chain Fv (scFv) antibody fragments, obtained by recombinant DNA techniques from the anti-carcinoembryonic antigen (CEA) monoclonal antibody (Mab) CB/ior-CEA. 1. This antibody has high affinity for CEA and is employed in the diagnosis and follow-up of human colorectal tumors. As the original Mab, the monovalent fragment and the diabody exhibit high affinity for human CEA and recognize an epitope dependent of carbohydrate conservation. The monovalent scFv fragment and the diabody have affinity constants for CEA of (5.0±0.4)×109 L mol−1 and (2.8±0.3)×1010 L mol−1, respectively. These two fragments do not show cross reactivity with human normal cells and tissues, exception made of the normal colonic mucosa, where CEA is occasionally present. The fragments can be produced through the expression in recombinant microorganisms, starting from the cloning of the encoding variable region nucleic acid sequences obtained from the hybridoma that produces Mab CB/ior-CEA.1. As the original Mab, the monovalent scFv and the diabody have the ability to identify in vivo cells that produce human CEA and grow as tumors in mice. The monovalent scFv and the diabody have a molecular size 5 and 2.5 times lower, respectively, than the mouse Mab, and do not have Fc domains, fact this that confers them the potential to better penetrate tissues in vivo and to be less immunogenic in man.

    摘要翻译： 本发明涉及通过来自抗癌胚抗原（CEA）单克隆抗体（Mab）CB / i-CEA的重组DNA技术获得的单价和二价（双抗体）单链Fv（scFv）抗体片段。 该抗体对CEA具有高亲和力，用于诊断和追踪人结肠直肠肿瘤。 作为原始的Mab，单价片段和双抗体对人CEA表现出高亲和力，并识别依赖于碳水化合物保护的表位。 单价scFv片段和双抗体对于（5.0±0.4）×10 9 L mol -1 -1和（2.8±0.3）×10 10 -1的CEA具有亲和力常数， / SUP> L mol 。 这两个片段不显示与人正常细胞和组织的交叉反应性，例外是正常结肠粘膜，其中CEA偶尔存在。 通过从产生Mab CB / i-CEA.1的杂交瘤获得的编码可变区核酸序列的克隆开始，可以通过重组微生物中的表达产生片段。 作为原始的Mab，单价scFv和双抗体具有鉴定产生人CEA的体内细胞并在小鼠中作为肿瘤生长的能力。 单价scFv和双抗体分别具有比小鼠Mab低5和2.5倍的分子量，并且不具有Fc结构域，事实上，这赋予它们在体内更好地穿透组织的潜力并且在人中具有较少的免疫原性 。

    Chlamydia pneumoniae polypeptides and uses thereof
    34.
    发明授权
    Chlamydia pneumoniae polypeptides and uses thereof 失效
    肺炎衣原体多肽及其用途

    公开(公告)号：US07101963B2

    公开(公告)日：2006-09-05

    申请号：US10289762

    申请日：2002-11-07

    申请人： Rémy Griffais ,  Susan K. Hoiseth ,  Robert J. Zagursky ,  Benjamin J. Metcalf ,  Joel A. Peek ,  Banumathi Sankaran ,  Leah D. Fletcher

    发明人： Rémy Griffais ,  Susan K. Hoiseth ,  Robert J. Zagursky ,  Benjamin J. Metcalf ,  Joel A. Peek ,  Banumathi Sankaran ,  Leah D. Fletcher

    IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K1/00 ,  C12Q1/68 ,  C12N15/00

    CPC分类号： C07K14/295 ,  A01K2217/05 ,  A61K39/00

    摘要： The subject of the invention is the genomic sequence and the nucleotide sequences encoding polypeptides of Chlamydia pneumoniae, such as cellular envelope polypeptides, which are secreted or specific, or which are involved in metabolism, in the replication process or in virulence, polypeptides encoded by such sequences, as well as vectors including the said sequences and cells or animals transformed with these vectors. The invention also relates to transcriptional gene products of the Chlamydia pneumoniae genome, such as, for example, antisense and ribozyme molecules, which can be used to control growth of the microorganism. The invention also relates to methods of detecting these nucleic acids or polypeptides and kits for diagnosing Chlamydia pneumoniae infection. The invention also relates to a method of selecting compounds capable of modulating bacterial infection and a method for the biosynthesis or biodegradation of molecules of interest using the said nucleotide sequences or the said polypeptides. The invention finally comprises, pharmaceutical, in particular vaccine, compositions for the prevention and/or treatment of bacterial, in particular Chlamydia pneumoniae, infections.

    摘要翻译： 本发明的主题是编码肺炎衣原体多肽的基因组序列和核苷酸序列，例如细胞包膜多肽，其在分泌或特异性中或在复制过程中或在毒力中参与代谢，由此编码的多肽 序列，以及包含所述序列的载体和用这些载体转化的细胞或动物。 本发明还涉及可用于控制微生物生长的肺炎衣原体基因组的转录基因产物，例如反义和核酶分子。 本发明还涉及检测这些核酸或多肽的方法以及用于诊断肺炎衣原体感染的试剂盒。 本发明还涉及使用所述核苷酸序列或所述多肽选择能够调节细菌感染的化合物的方法和用于生物合成或生物降解感兴趣分子的方法。 本发明最终包括用于预防和/或治疗细菌，特别是肺炎衣原体感染的药物，特别是疫苗组合物。