公开(公告)号：US5417863A

公开(公告)日：1995-05-23

申请号：US269952

申请日：1994-06-30

申请人： Laszlo Varady ,  Noubar B. Afeyan ,  Robert Langer ,  Shmuel Shefer

发明人： Laszlo Varady ,  Noubar B. Afeyan ,  Robert Langer ,  Shmuel Shefer

IPC分类号： G01N33/50 ,  C12Q1/60 ,  G01N33/92 ,  B01D15/08

CPC分类号： C12Q1/60 ,  G01N33/92 ,  G01N2800/044 ,  Y10T436/255

摘要： Disclosed is a method for the rapid quantitative determination of low density lipoprotein (LDL) in a biological fluid sample containing LDL, very low density lipoprotein (VLDL) and high density lipoprotein (HDL). The sample is first passed through a matrix to bind LDL to the matrix. The LDL is then eluted from the matrix differentially from VLDL and HDL. A parameter representative of the concentration of a solute exiting the matrix during the interval when LDL is eluted is then determined, wherein the parameter is proportional to the amount of the LDL in the sample. The parameter may be determined, e.g., by electronically integrating under a spectrophotometrically detected peak of eluted LDL.

摘要翻译： 公开了一种用于在含有LDL，极低密度脂蛋白（VLDL）和高密度脂蛋白（HDL））的生物液体样品中快速定量测定低密度脂蛋白（LDL）的方法。 样品首先通过矩阵将LDL结合到基质上。 然后将LDL从VLDL和HDL差异性地从基质中洗脱出来。 然后确定表示在洗脱LDL的间隔期间离开基质的溶质的浓度的参数，其中参数与样品中LDL的量成比例。 该参数可以例如通过在分光光度法检测的洗脱的LDL的峰上电子积分来确定。

公开(公告)号：US5389449A

公开(公告)日：1995-02-14

申请号：US467

申请日：1993-01-05

申请人： Noubar B. Afeyan ,  Hossein K. Hodjat ,  Laszlo Varady

发明人： Noubar B. Afeyan ,  Hossein K. Hodjat ,  Laszlo Varady

IPC分类号： B01J20/281 ,  B01J20/32 ,  B01J41/14 ,  G01N30/88 ,  B01D15/08 ,  B05D3/10 ,  C08F8/32 ,  C08F8/38

CPC分类号： B01J41/125 ,  B01J41/20 ,  Y10T428/2991 ,  Y10T428/2998 ,  Y10T428/31931 ,  Y10T428/31938

摘要： A method of derivatizing a chromatography matrix having a hydrophobic surface involves reacting the hydrophobic surface with a halosulfonating agent to produce a significant amount of halosulfone groups covalently bonded onto the surface. Sulfonamide bonds then are formed between these halosulfone groups and a group of amine functions on a polyaminated polymer to produce a pellicular anion exchange layer covalently bonded to the surface. The resulting layer is stable in the absence of cross-links between the molecules of the polyaminated polymer.

摘要翻译： 衍生具有疏水性表面的色谱基质的方法包括使疏水表面与卤代磺化剂反应以产生大量共价键合到表面上的卤代烃基团。 然后在这些卤代烃基之间形成磺酰胺键，并在聚氨酯聚合物上形成一组胺官能团，以产生共价结合到表面上的薄膜状阴离子交换层。 所得层在多层聚合物的分子之间不存在交联的情况下是稳定的。

公开(公告)号：US5306426A

公开(公告)日：1994-04-26

申请号：US67418

申请日：1993-05-25

申请人： Noubar B. Afeyan

发明人： Noubar B. Afeyan

IPC分类号： B01D15/08 ,  G01N30/06 ,  G01N30/08 ,  G01N30/14 ,  G01N30/88

CPC分类号： G01N30/08 ,  G01N30/14 ,  G01N2030/062 ,  G01N2030/085 ,  G01N2030/143 ,  G01N2030/8813 ,  G01N2030/8831 ,  G01N2030/8872

摘要： The invention features a method of detecting a trace solute in a solution which contains a major amount of a dissolved product, the method including the steps of: flowing the solution through means for extracting the product to produce an effluent flow substantially free of product containing the trace solute; flowing the effluent through a trace solute adsorbing means to progressively accumulate therein the trace solute; and eluding the trace solute from the adsorber to produce an eluant fraction containing a detectable quantity of the trace solute.

摘要翻译： 本发明的特征在于一种检测溶液中痕量溶质的方法，该溶液含有主要量的溶解产物，该方法包括以下步骤：使溶液流过提取产物的装置，以产生基本上不含含有 痕量溶质 使污水流过痕量溶质吸附装置，逐渐积累痕量溶质; 并且从吸附器中排除痕量溶质以产生含有可检测量的痕量溶质的洗脱液组分。

公开(公告)号：US6133444A

公开(公告)日：2000-10-17

申请号：US487666

申请日：1995-06-07

申请人： James M. Coull ,  Michael Egholm ,  Richard P. Hodge ,  Mohamed Ismail ,  Sharanappa B. Rajur

发明人： James M. Coull ,  Michael Egholm ,  Richard P. Hodge ,  Mohamed Ismail ,  Sharanappa B. Rajur

IPC分类号： C07D239/47 ,  C07D239/46 ,  C07D473/18 ,  C07D473/34 ,  C07D473/40 ,  C07K14/00

CPC分类号： C07D239/47 ,  C07D473/18 ,  C07D473/34 ,  C07D473/40 ,  C07K14/003 ,  Y02P20/55

摘要： A method is disclosed for preparing novel purine PNA synthons having protecting groups which may be removed under mild conditions. The purine PNA synthons generally are prepared by coupling purine derivatives having carbamate protection to a protected N-(2-aminoethyl)-glycine backbone. By a method of this invention, purine PNA synthons may have orthogonal protection of the carbamate protected purine and the protected backbone. The purine PNA synthons are useful in the synthesis of peptide nucleic acids (PNAs) and other oligomers such as PNA-DNA chimeras, and may be used in automated synthesizers. In practicing methods of the invention, novel compositions of matter also are disclosed. For example, disclosed herein are an adenine PNA synthon having the following formula: ##STR1## and a guanine PNA synthon having the following formula: ##STR2##

摘要翻译： 公开了一种制备具有可在温和条件下除去的保护基的新型嘌呤PNA合成子的方法。 嘌呤PNA合成酶通常通过将具有氨基甲酸酯保护的嘌呤衍生物与保护的N-（2-氨基乙基） - 甘氨酸主链偶联来制备。 通过本发明的方法，嘌呤PNA合成子可具有氨基甲酸酯保护的嘌呤和被保护的主链的正交保护。 嘌呤PNA合成子可用于合成肽核酸（PNA）和其它寡聚体如PNA-DNA嵌合体，并可用于自动合成仪中。 在本发明的实践方法中，还公开了新的物质组合物。 例如，本文公开了具有下式的腺嘌呤PNA合成子和具有下式的鸟嘌呤PNA合成子：

公开(公告)号：US06063569A

公开(公告)日：2000-05-16

申请号：US910552

申请日：1997-08-11

申请人： Brian D. Gildea ,  James M. Coull

发明人： Brian D. Gildea ,  James M. Coull

IPC分类号： C12N15/09 ,  A61K31/712 ,  A61K48/00 ,  C07C271/20 ,  C07D239/46 ,  C07D239/47 ,  C07D239/54 ,  C07D473/18 ,  C07D473/32 ,  C07D473/34 ,  C07D473/40 ,  C07H21/00 ,  C07H21/04 ,  C07K14/00 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/02

CPC分类号： C07D239/47 ,  C07D473/18 ,  C07D473/32 ,  C07D473/40 ,  C07H21/00 ,  C07K14/003 ,  Y02P20/55

摘要： A method is disclosed for the preparation of novel PNA synthons compatible with DNA synthetic reagents and instrumentation. Accordingly, the PNA synthons of this invention are particularly suitable for the preparation of PNA-DNA chimeras, among other oligomers. The PNA synthons are designed to have a protecting group strategy which is orthogonal and allows removal of the protecting groups under mild conditions. Generally, an acid labile protected backbone is coupled to a nucleobase side chain moiety to form the PNA synthon. A novel method for synthesizing the acid labile protected backbone also is described. In addition, novel compositions of matter are disclosed.

摘要翻译： 公开了一种用于制备与DNA合成试剂和仪器相容的新型PNA合成子的方法。 因此，本发明的PNA合成子特别适用于制备PNA-DNA嵌合体以及其它低聚物。 PNA合成子被设计成具有正交的保护基团策略，并允许在温和条件下去除保护基团。 通常，酸不稳定保护的主链与核碱基侧链部分偶联以形成PNA合成子。 还描述了一种合成酸不稳定保护骨架的新方法。 另外，公开了新的物质组合物。

公开(公告)号：US5885775A

公开(公告)日：1999-03-23

申请号：US726090

申请日：1996-10-04

申请人： Lawrence A. Haff ,  Igor Pavlovich Smirnov

发明人： Lawrence A. Haff ,  Igor Pavlovich Smirnov

IPC分类号： C12Q1/68 ,  C12P19/34 ,  G01N24/00

CPC分类号： C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/16 ,  Y10T436/24

摘要： The invention relates to methods for determining sequence information in polynucleotides by combining the recent disparate technologies of mass spectrometry and polynucleotide hybridization, amplification, extension and/or ligation techniques. Broadly, in a first step, the method for determining sequence information in a sample polynucleotide includes hybridizing with a sample nucleotide one or a mixture of oligonucleotide probes having a nucleotide sequence complementary to a portion of the sample polynucleotide, thereby forming a complex. Then, in a second step, the complex is contacted with at least a member selected from the group consisting of nucleosides, dideoxynucleosides, polymerases, nucleases, transcriptases, ligases and restriction enzymes to alter at least a subset of said oligonucleotide probes. In a third step, the method provides for determining the molecular weight of at least the subset of altered probes by mass spectrometry and infering the sequence information of the sample polynucleotide therefrom.

摘要翻译： 本发明涉及通过组合最近不同的质谱技术和多核苷酸杂交，扩增，延伸和/或连接技术来确定多核苷酸序列信息的方法。 广泛地，在第一步中，用于确定样品多核苷酸中的序列信息的方法包括与样品核苷酸1或具有与样品多核苷酸的一部分互补的核苷酸序列的寡核苷酸探针的混合物杂交，从而形成复合物。 然后，在第二步中，将复合物与至少一种选自核苷，双脱氧核苷，聚合酶，核酸酶，转录酶，连接酶和限制酶的成员接触以改变至少一个所述寡核苷酸探针的子集。 在第三步中，所述方法提供通过质谱法确定至少所述改变的探针子集的分子量，并从其中推断样品多核苷酸的序列信息。

公开(公告)号：US5777077A

公开(公告)日：1998-07-07

申请号：US803557

申请日：1997-02-20

申请人： Fernando Albericio ,  Steven A. Kates

发明人： Fernando Albericio ,  Steven A. Kates

IPC分类号： B01J19/00 ,  B01J23/44 ,  B01J31/24 ,  B01J31/28 ,  C07B61/00 ,  C07K1/06 ,  C07K1/113 ,  C07K1/12 ,  C40B40/10 ,  C40B60/14

CPC分类号： C07K1/122 ,  B01J19/0046 ,  B01J23/44 ,  B01J31/0237 ,  B01J31/2404 ,  C07K1/12 ,  B01J2219/00286 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00725 ,  B01J2231/60 ,  B01J2531/824 ,  B01J31/04 ,  C40B40/10 ,  C40B60/14 ,  Y02P20/55

摘要： A method for removing an allyl protecting group from an allyl-protected derivative of a biologically relevant amino acid is disclosed. One relevant aspect of the method is the use of soluble organometallic catalyst, such as an organopalladium catalyst. Preferably, soluble tetrakistriphenylphosphine palladium (0) is used. The allyl deprotection method now disclosed is suitable for use on an instrument for automated peptide synthesis. Methods of preparing the soluble organometallic catalyst are also disclosed, as are soluble catalyst compositions.

公开(公告)号：US5757482A

公开(公告)日：1998-05-26

申请号：US425290

申请日：1995-04-20

申请人： Martin Fuchs ,  Lance Bryant Koutny

发明人： Martin Fuchs ,  Lance Bryant Koutny

IPC分类号： G01N21/05 ,  G01N21/03

CPC分类号： G01N21/05 ,  G01N2021/0346

摘要： Disclosed is an apparatus for fluidic separation systems comprising a microfabricated conduit including a usefully long optical pathlength. The provision of a usefully long optical pathlength in the apparatus greatly improves the sensitivity of detection of separated analytes without compromising the resolving power of the apparatus.

摘要翻译： 公开了一种用于流体分离系统的装置，其包括具有有用长的光程长度的微细导管。 在设备中提供有用的长光程长大大提高了检测分离的分析物的灵敏度，而不会影响设备的分辨率。

公开(公告)号：US5641539A

公开(公告)日：1997-06-24

申请号：US457326

申请日：1995-06-01

申请人： Noubar B. Afeyan ,  Laszlo Varady ,  Fred Regnier

发明人： Noubar B. Afeyan ,  Laszlo Varady ,  Fred Regnier

IPC分类号： B01J20/285 ,  B01D15/08 ,  B01J20/22 ,  B01J20/30 ,  B01J20/32 ,  G01N30/88 ,  G01N33/543 ,  G01N33/545

CPC分类号： G01N33/54353 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/3285 ,  G01N33/545 ,  G01N2600/00

摘要： Disclosed are chemically-produced specific binding, "molecular imaged" sorbents which reversibly bind a preselected macromolecule by spatially matched multipoint interactions between functional groups synthesized on the surface of the sorbent and functional groups on the surface of the macromolecule. Also disclosed are methods of producing such sorbents. The sorbents typically are high surface area solids comprising surface binding regions which have charged groups, metal coordinating groups, hydrophobic moieties, or various combination thereof anchored thereto and spaced in the mirror image of complementary interactive groups on a surface of the macromolecule.

摘要翻译： 公开了化学产生的特异性结合，“分子成像”吸附剂，其通过在吸附剂表面上合成的官能团和大分子表面上的官能团之间的空间匹配的多点相互作用可逆地结合预选的大分子。 还公开了生产这种吸附剂的方法。 吸附剂通常是高表面积固体，其包含具有带电基团，金属配位基团，疏水部分或锚定于其上的各种组合并且在大分子表面上的互补相互作用基团的镜像中隔开的表面结合区域。

公开(公告)号：US5630924A

公开(公告)日：1997-05-20

申请号：US425828

申请日：1995-04-20

申请人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

发明人： Martin Fuchs ,  Wassim A. Nashabeh ,  Dieter R. Schmalzing

IPC分类号： G01N33/533 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/537 ,  G01N33/561 ,  G01N33/563 ,  G01N37/00 ,  C25B7/00

CPC分类号： G01N27/44726 ,  C12Q1/68 ,  G01N33/5375 ,  G01N33/561 ,  G01N2333/59

摘要： Compositions, methods, and apparatus for performing ultrafast binding assays by capillary electrophoresis or other electroseparation techniques are disclosed. In one embodiment, a first binding partner carries a detectable label and a second binding partner is modified to be highly charged. When used in combination with a sample containing an analyte with which both binding partners can interact and bind thereto, a three-membered complex is formed. The electrophoretic mobility difference between the unbound and complex-bound forms of labeled first binding partner is such that electroseparation and subsequent detection of an analyte can be accomplished. The compositions, methods, and apparatus disclosed herein also permit quantitative determination of the concentration of an analyte in a sample.

摘要翻译： 公开了通过毛细管电泳或其他电离分离技术进行超快速结合测定的组合物，方法和装置。 在一个实施方案中，第一结合伴侣携带可检测标记，第二结合伴侣被修饰为高度带电。 当与含有两个结合配偶体可以与其结合的分析物的样品组合使用时，形成三元复合物。 标记的第一结合配偶体的未结合和复合结合形式之间的电泳迁移率差异可以实现电分离和随后的分析物检测。 本文公开的组合物，方法和装置还允许定量测定样品中分析物的浓度。