公开(公告)号：US07244559B2

公开(公告)日：2007-07-17

申请号：US09814338

申请日：2001-03-21

Applicant: Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

Inventor： Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: G01N21/6452 ,  B01L3/5027 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B01L2300/0893 ,  B82Y15/00 ,  B82Y30/00 ,  C12P19/34 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/0303 ,  G01N21/7703 ,  C12Q2565/519 ,  C12Q2533/101 ,  C12Q2531/125 ,  C12Q2535/101 ,  C12Q2565/301 ,  C12Q2565/107 ,  C12Q2525/307

Abstract: Disclosed herein are methods and apparatuses for sequencing a nucleic acid. These methods permit a very large number of independent sequencing reactions to be arrayed in parallel, permitting simultaneous sequencing of a very large number (>10,000) of different oligonucleotides.

公开(公告)号：US07211390B2

公开(公告)日：2007-05-01

申请号：US10788529

申请日：2004-02-26

Applicant: Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

Inventor： Jonathan M. Rothberg ,  Joel S. Bader ,  Scott B. Dewell ,  Keith McDade ,  John W. Simpson ,  Jan Berka ,  Christopher M. Colangelo

IPC: C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC classification number: C12Q1/6837 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0877 ,  B82Y30/00 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/6452 ,  G01N2201/08 ,  G01N2201/0846 ,  C12Q2531/125 ,  C12Q2535/101 ,  C12Q2565/301 ,  C12Q2565/107 ,  C12Q2525/307

Abstract: Disclosed herein are methods and apparatuses for sequencing a nucleic acid. In one aspect, the method includes annealing a population of circular nucleic acid molecules to a plurality of anchor primers linked to a solid support, and amplifying those members of the population of circular nucleic acid molecules which anneal to the target nucleic acid, and then sequencing the amplified molecules by detecting the presence of a sequence byproduct such as pyrophosphate.

Abstract translation: 本文公开了用于测序核酸的方法和装置。 一方面，该方法包括将多个环状核酸分子退火至与固体支持物连接的多个锚定引物，并扩增与靶核酸退火的环状核酸分子群体的那些成员，然后测序 通过检测序列副产物如焦磷酸盐的存在来扩增分子。

公开(公告)号：US20200348794A1

公开(公告)日：2020-11-05

申请号：US16859051

申请日：2020-04-27

Applicant: Tyler S. Ralston ,  Jianwei Liu ,  Jonathan M. Rothberg

Inventor： Tyler S. Ralston ,  Jianwei Liu ,  Jonathan M. Rothberg

IPC: G06F3/043 ,  G06F3/041 ,  G06F3/0488

Abstract: An electronic device with an ability to perform touch detection includes an ultrasound touch sensor disposed within a housing and configured to detect physical contact with an exterior surface of the housing. The sensor may emit ultrasonic sound waves, receive reflected ultrasonic sound waves reflected from a surface portion corresponding to the physical contact at the exterior surface of the housing, and compare the reflected ultrasonic sound waves to a stored reflection pattern. Based on determining a match between the reflected ultrasonic sound waves and the stored reflection pattern, the electronic device may activate a function. The sensor may be part of an ultrasound-on-a-chip device.

公开(公告)号：US20170365774A1

公开(公告)日：2017-12-21

申请号：US15626330

申请日：2017-06-19

Applicant: Jonathan M. Rothberg ,  Susan A. Alie ,  Jaime Scott Zahorian ,  Paul Francis Cristman ,  Keith G. Fife

Inventor： Jonathan M. Rothberg ,  Susan A. Alie ,  Jaime Scott Zahorian ,  Paul Francis Cristman ,  Keith G. Fife

IPC: H01L41/27 ,  H01L41/047 ,  B06B1/06 ,  H05K3/36

CPC classification number: H01L41/27 ,  B06B1/0292 ,  B06B1/06 ,  B06B1/0622 ,  H01L41/047 ,  H05K3/361 ,  H05K2201/10977 ,  H05K2203/0726

Abstract: An ultrasound-on-a-chip device has an ultrasonic transducer substrate with plurality of transducer cells, and an electrical substrate. For each transducer cell, one or more conductive bond connections are disposed between the ultrasonic transducer substrate and the electrical substrate. Examples of electrical substrates include CMOS chips, integrated circuits including analog circuits, interposers and printed circuit boards.

公开(公告)号：US09269708B2

公开(公告)日：2016-02-23

申请号：US13245675

申请日：2011-09-26

Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/403 ,  H01L27/088 ,  C12Q1/68 ,  H01L29/78 ,  G01N27/414

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US08530941B2

公开(公告)日：2013-09-10

申请号：US13022678

申请日：2011-02-08

Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/403

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US08492799B2

公开(公告)日：2013-07-23

申请号：US13149250

申请日：2011-05-31

Applicant: Jonathan M Rothberg ,  Wolfgang Hinz

Inventor： Jonathan M Rothberg ,  Wolfgang Hinz

IPC: G01N27/403

CPC classification number: C12Q1/6825 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/4145 ,  G01N27/4148 ,  G01N33/54373 ,  G01N33/6818 ,  H01L29/78 ,  H01L29/788 ,  H01L2924/01006

Abstract: Methods and apparatuses relating to large scale FET arrays for analyte detection and measurement are provided. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes.

公开(公告)号：US20130078638A1

公开(公告)日：2013-03-28

申请号：US13618334

申请日：2012-09-14

Applicant: Jan Berka ,  Yi-Ju Chen ,  John H. Leamon ,  Steve Lefkowitz ,  Kenton L. Lohman ,  Vinod B. Makhijani ,  Jonathan M. Rothberg ,  Gary J. Sarkis ,  Maithreyan Srinivasan ,  Michael P. Weiner

Inventor： Jan Berka ,  Yi-Ju Chen ,  John H. Leamon ,  Steve Lefkowitz ,  Kenton L. Lohman ,  Vinod B. Makhijani ,  Jonathan M. Rothberg ,  Gary J. Sarkis ,  Maithreyan Srinivasan ,  Michael P. Weiner

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5085 ,  B01L7/52 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0877 ,  C07H21/00 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6865 ,  C12Q1/6867 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N21/253 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2021/6484 ,  G01R33/1269 ,  Y02P20/582 ,  Y10T436/143333 ,  C12Q2565/119 ,  C12Q2531/113 ,  C12Q2565/537 ,  C12Q2547/107 ,  C12Q2547/101 ,  C12Q2537/143 ,  C12Q2527/125 ,  C12Q2525/186 ,  C12Q2565/301 ,  C12Q2563/149 ,  C12Q2531/125 ,  C12Q2537/149 ,  C12Q2535/119

Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Abstract translation: 公开了核酸扩增的方法，其中将核酸模板，珠粒和扩增反应溶液乳化并扩增核酸模板以提供附着于头部的核酸模板的克隆拷贝。 还公开了用于执行本发明的方法的套件和装置。

公开(公告)号：US08269261B2

公开(公告)日：2012-09-18

申请号：US13022694

申请日：2011-02-08

Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: G01N27/403

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

公开(公告)号：US20120129732A1

公开(公告)日：2012-05-24

申请号：US13019517

申请日：2011-02-02

Applicant: Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo

IPC: C40B60/12

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.