公开(公告)号：US09952417B2

公开(公告)日：2018-04-24

申请号：US15627866

申请日：2017-06-20

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G02B21/00 ,  G02B21/36 ,  G01N15/10 ,  G01N15/14 ,  G06K9/00 ,  G01N33/80 ,  G01T1/02 ,  G01B9/02 ,  G06T7/00 ,  G03H1/04 ,  G01N11/00 ,  G03H1/00 ,  G01N15/00 ,  C12M1/34

CPC classification number: G02B21/0008 ,  C12M41/36 ,  G01B9/02041 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1463 ,  G01N15/1475 ,  G01N33/80 ,  G01N2011/008 ,  G01N2015/0069 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/149 ,  G01T1/02 ,  G02B21/00 ,  G02B21/0004 ,  G02B21/361 ,  G02B21/365 ,  G03H2001/005 ,  G03H2001/045 ,  G06K9/00127 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/30242 ,  Y10S435/808

Abstract: Among other things, a method comprises imaging a sample displaced between a sensor surface and a surface of a microscopy sample chamber to produce an image of at least a part of the sample. The image is produced using lensless optical microscopy, and the sample contains at least blood from a subject. The method also comprises automatically differentiating cells of different types in the image, generating a count of one or more cell types based on the automatic differentiation, and deriving a radiation dose the subject has absorbed based on the count.

公开(公告)号：US20160187235A1

公开(公告)日：2016-06-30

申请号：US15066065

申请日：2016-03-10

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Hershel Macaulay

IPC: G01N1/28 ,  G01N21/31 ,  G02B21/36 ,  G01N1/38 ,  G01N33/49

Abstract: Among other things, a diluted sample is generated based on mixing a small sample of blood with a one or more diluents. A thin film of the diluted sample is formed on the surface of a contact optical microscopy sensor. Red blood cells within a portion of the thin film of the diluted sample are illuminated using light of a predetermined wavelength. One or more images of the diluted sample are acquired based on illuminating the red blood cells within the portion of the thin film of the diluted sample. The acquired one or more images of the diluted sample are then processed. The mean corpuscular hemoglobin in the red blood cells within the portion of the thin film of the diluted sample is determined based on processing the acquired images of the diluted sample.

Abstract translation: 除此之外，基于将小样本的血液与一种或多种稀释剂混合而产生稀释的样品。 在接触式光学显微镜传感器的表面上形成稀薄样品的薄膜。 使用预定波长的光照射稀释样品的薄膜部分内的红细胞。 基于稀释样品的薄膜部分内的红细胞的照射，获得稀释样品的一个或多个图像。 然后处理所获得的稀释样品的一个或多个图像。 基于稀释样品的获取图像的处理，确定稀释样品薄膜部分内红细胞中的平均红细胞血红蛋白。

公开(公告)号：US20240385184A1

公开(公告)日：2024-11-21

申请号：US18674179

申请日：2024-05-24

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N21/17 ,  G01N21/41 ,  G01N21/55 ,  G01N21/59 ,  G01N33/569

Abstract: A method includes attaching two or more beads to each unit of one or more units of a chemical component in a sample, to form, for each unit of the chemical component, a multi-bead complex including two or more beads and the unit of the chemical component; placing the sample on a surface of an image sensor; at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the beads of the multi-bead complexes; at the image sensor, capturing one or more images of the sample from the received light; and identifying, in at least one of the images of the sample, separate multi-bead complexes, the identifying of the separate multi-bead complexes including associating the two or more beads of each of the multi-bead complexes based on proximity to one another.

公开(公告)号：US11874452B2

公开(公告)日：2024-01-16

申请号：US17696704

申请日：2022-03-16

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Hershel Macaulay ,  Noah Hymes-Vandermeulen

IPC: G01N21/59 ,  G02B21/34 ,  G01N1/40 ,  G01N15/06 ,  G01N1/28 ,  G01N15/14 ,  G01N33/49 ,  G01N35/00 ,  G01N15/00 ,  G01N15/10

CPC classification number: G02B21/34 ,  G01N1/28 ,  G01N1/4077 ,  G01N15/0612 ,  G01N15/1468 ,  G01N21/59 ,  G01N33/49 ,  G01N15/0606 ,  G01N35/00029 ,  G01N2015/008 ,  G01N2015/1087 ,  G01N2015/1486

Abstract: Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.

公开(公告)号：US20230288407A1

公开(公告)日：2023-09-14

申请号：US18200301

申请日：2023-05-22

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/569 ,  G01N21/41 ,  G01N21/55 ,  G01N21/59

CPC classification number: G01N33/54313 ,  G01N21/41 ,  G01N21/55 ,  G01N21/59 ,  G01N33/56983 ,  G01N2021/1772

Abstract: A method includes attaching two or more beads to each unit of one or more units of a chemical component in a sample, to form, for each unit of the chemical component, a multi-bead complex including two or more beads and the unit of the chemical component; placing the sample on a surface of an image sensor; at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the beads of the multi-bead complexes; at the image sensor, capturing one or more images of the sample from the received light; and identifying, in at least one of the images of the sample, separate multi-bead complexes, the identifying of the separate multi-bead complexes including associating the two or more beads of each of the multi-bead complexes based on proximity to one another.

公开(公告)号：US20230228682A1

公开(公告)日：2023-07-20

申请号：US18188750

申请日：2023-03-23

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N21/64 ,  G01Q60/20

CPC classification number: G01N21/6458 ,  G01N21/6408 ,  G01Q60/20 ,  G01N2201/062 ,  G02B21/16

Abstract: Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

公开(公告)号：US20220412968A1

公开(公告)日：2022-12-29

申请号：US17674967

申请日：2022-02-18

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/569 ,  G01N21/41 ,  G01N21/55 ,  G01N21/59

Abstract: A method includes attaching two or more beads to each unit of one or more units of a chemical component in a sample, to form, for each unit of the chemical component, a multi-bead complex including two or more beads and the unit of the chemical component; placing the sample on a surface of an image sensor; at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the beads of the multi-bead complexes; at the image sensor, capturing one or more images of the sample from the received light; and identifying, in at least one of the images of the sample, separate multi-bead complexes, the identifying of the separate multi-bead complexes including associating the two or more beads of each of the multi-bead complexes based on proximity to one another.

公开(公告)号：US11294160B2

公开(公告)日：2022-04-05

申请号：US17085389

申请日：2020-10-30

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine ,  Peter Hodges Gregson

IPC: G02B21/00

Abstract: Among other things, an imaging device has a photosensitive array of pixels, and a surface associated with the array is configured to receive a specimen with at least a part of the specimen at a distance from the surface equivalent to less than about half of an average width of the pixels.

公开(公告)号：US20220082557A1

公开(公告)日：2022-03-17

申请号：US17409478

申请日：2021-08-23

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/50 ,  G01N21/77 ,  G01N33/53

Abstract: Among other things, two or more different antibodies are caused to bind to one or more units of a chemical component in a sample. Each of the antibodies is attached to one or more beads (e.g., microbeads). The sample is situated on a surface of an image sensor. At the image sensor, light is received originating at a light source that is other than the beads. The received light includes light reflected by, refracted by, or transmitted through the beads. At least one image of the sample is processed to separately enumerate individual beads and complexes of two or more of the beads attached to the two or more antibodies that are bound to a unit of the chemical component. The results of the processing are used to identify a presence or a level of the chemical component in the sample.

公开(公告)号：US20210311038A1

公开(公告)日：2021-10-07

申请号：US17349710

申请日：2021-06-16

Applicant: Alentic Microscience Inc.

Inventor： Alan Marc Fine

IPC: G01N33/543 ,  G01N33/50 ,  G01N21/77 ,  G01N33/53

Abstract: An indicator of a first type and an indicator of a second type are attached to a unit of a chemical component in a sample to form a first multi-indicator complex. The first multi-indicator complex includes the unit of the chemical component, the indicator of the first type, and the indicator of the second type. The indicator of the first type and the indicator of the second type have different discernible characteristics. An image of the sample, including the first multi-indicator complex corresponding to the unit of the chemical component, is captured by an image sensor. Based on a first image of the sample, a count is generated of multi-indicator complexes that include an indicator of the first type and an indicator of the second type, including the first multi-indicator complex. Based on the count, a presence or a level of the chemical component in the sample is identified.