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公开(公告)号：US20170160284A1
公开(公告)日：2017-06-08
申请号：US15392295
申请日：2016-12-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Levente EGRY , Kyle GEE
Abstract: According to the present teachings, systems, compositions, kits and methods for protein melt analysis are provided that utilizing a dye that is a dipyrrometheneboron difluoride compound. In some embodiments, a method comprises preparing a sample by mixing at least one protein with a dye, and applying a controlled heating, while recording the fluorescence emission of the sample.
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公开(公告)号：US20160305964A1
公开(公告)日：2016-10-20
申请号：US15152346
申请日：2016-05-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle GEE , Vladimir MARTIN
IPC: G01N33/84 , C07C311/44 , C09B11/08 , G01N33/20 , C09B19/00 , C07K14/435 , C07K14/195 , C07C229/18 , C09B57/08
CPC classification number: G01N33/84 , C07C229/18 , C07C311/44 , C07D209/14 , C07D221/14 , C07D265/38 , C07D311/82 , C07D311/90 , C07D413/04 , C07K14/195 , C07K14/43595 , C09B5/60 , C09B5/62 , C09B11/06 , C09B11/08 , C09B19/00 , C09B19/005 , C09B57/08 , C09K11/06 , C09K2211/1014 , G01N33/20 , G01N2458/00
Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.
Abstract translation: 本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。 本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。
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公开(公告)号：US20160139158A1
公开(公告)日：2016-05-19
申请号：US14937736
申请日：2015-11-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jeffrey DZUBAY , Kyle GEE , Vladimir MARTIN , Aleksey RUKAVISHNIKOV , Daniel BEACHAM
CPC classification number: G01N33/84 , C07D209/14 , C07D311/82 , C07D311/90 , C07D405/12 , C07D413/04 , C07D491/22 , C07F5/02 , C07K1/13 , C09B7/00 , C09B11/12 , C09B11/24 , C09B11/28 , C09B57/00 , C09B69/00 , C09K11/06 , G01N31/22 , G01N33/5005 , G01N33/5058 , G01N33/5091 , G01N33/582 , G01N33/80 , G01N2333/245 , Y10T436/143333
Abstract: A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: wherein the variables are described throughout the application.
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Patent Agency Ranking
公开(公告)号：US20160031821A1
公开(公告)日：2016-02-04
申请号：US14810766
申请日：2015-07-28
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle GEE , Aimei CHEN , Hee Chol KANG
IPC: C07D225/08 , C07D225/02 , C07C309/42
CPC classification number: C07D225/08 , C07C309/42 , C07C2603/36 , C07D223/08 , C07D223/26 , C07D225/02 , C07K1/13 , C07K16/18 , G01N33/532
Abstract: The present disclosure is directed to a reactive ester agent for conjugating a click-reactive group to a carrier molecule or solid support. The reactive ester agent has the general formula IA, wherein the variables R1, R2, R3, Ra and L are described throughout the application.
Abstract translation: 本公开涉及用于将点击反应性基团与载体分子或固体支持物缀合的反应性酯试剂。 反应性酯剂具有通式IA，其中在整个申请中描述了变量R1，R2，R3，Ra和L。
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公开(公告)号：US20160010145A1
公开(公告)日：2016-01-14
申请号：US14649370
申请日：2013-12-06
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason DALLWIG , Kyle GEE , Nancy AHNERT , Hee Chol KANG , Jongtae YANG
CPC classification number: C12Q1/6825 , C09B23/00 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/086 , C12Q1/6816 , C12Q1/6837 , C12Q2525/207 , C12Q2563/107 , C12Q2563/173
Abstract: Substituted unsymmetrical cyanine dyes are provided herein, including kits, assays systems and methods for their use in the detection of RNA compounds (e.g., miRNA).
Abstract translation: 本文提供取代的不对称花青染料，包括用于检测RNA化合物（例如miRNA）的试剂盒，测定系统和方法。
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公开(公告)号：US20150051096A1
公开(公告)日：2015-02-19
申请号：US14330727
申请日：2014-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian AGNEW , Kyle GEE , Tamara NYBERG
IPC: G01N33/533 , G01N33/58 , G01N33/532 , C12P21/00
CPC classification number: C12P21/005 , A61K47/549 , A61K47/61 , C07K1/13 , C07K14/47 , C07K16/00 , C07K2317/14 , C07K2317/24 , C07K2317/41 , C12Y302/01096 , G01N33/5005 , G01N33/5008 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/534 , G01N33/581 , G01N33/582 , G01N33/583 , Y10T436/17
Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.
Abstract translation: 在某些实施方案中提供了形成报道分子，载体分子或固体支持物的叠氮基改性的生物分子缀合物的新方法。 在其它实施方案中提供了用叠氮化物基团对生物分子进行酶标记的方法。
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47.发明申请公开(公告)号：US20140356873A1
公开(公告)日：2014-12-04
申请号：US14462147
申请日：2014-08-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Zhenjun DIWU , Jixiang LIU , Kyle GEE
IPC: G01N33/58 , C07D491/044 , C07D471/04 , C12Q1/68 , C07D498/14 , C07D491/147 , C07D491/22 , C07D495/04 , C07D495/22
CPC classification number: G01N33/582 , C07D471/04 , C07D491/04 , C07D491/044 , C07D491/14 , C07D491/147 , C07D491/22 , C07D495/04 , C07D495/14 , C07D495/22 , C07D498/14 , C12Q1/6816 , Y10S436/80 , Y10T436/141111 , Y10T436/143333 , Y10T436/145555 , C12Q2563/107
Abstract: The present invention describes novel dyes, including coumarins, rhodamines, and rhodols that incorporate additional fused aromatic rings. The dyes of the invention absorb at a longer wavelength than structurally similar dyes that do not possess the fused aromatic rings. Many of the dyes of the invention are useful fluorescent dyes. The invention includes chemically reactive dyes, dye-conjugates, and the use of such dyes in staining samples and detecting ligands or other analytes.
Abstract translation: 本发明描述了新颖的染料，包括掺入另外的稠合芳环的香豆素，罗丹明和罗丹醇。 本发明的染料比不具有稠合芳环的结构相似的染料长的波长吸收。 本发明的许多染料是有用的荧光染料。 本发明包括化学活性染料，染料共轭物，以及这些染料在染色样品和检测配体或其他分析物中的用途。
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公开(公告)号：US20140045171A1
公开(公告)日：2014-02-13
申请号：US13965566
申请日：2013-08-13
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle GEE , Vladimir MARTIN
IPC: G01N21/75
CPC classification number: G01N21/75 , C07D263/56 , C07D263/57 , C07D401/04 , C07D413/04 , C07D417/04 , C07D471/06 , C07D491/16 , C09B57/00 , C09B57/02 , C09K11/06 , C09K2211/1007 , C09K2211/1014 , C09K2211/1029 , C09K2211/1033 , C09K2211/1037 , C09K2211/1048 , C09K2211/1088 , G01N33/582 , G01N33/84
Abstract: Disclosed are benzoxazole-based compounds, kits, and methods of producing and using the described compounds in fluorescence-based detection of analytes (e.g., metal ions). Also disclosed are uses of benzoxazole-based compounds as ratiometric metal ion indicators.
Abstract translation: 公开了基于苯并恶唑的化合物，试剂盒以及在分析物（例如金属离子）的基于荧光的检测中制备和使用所述化合物的方法。 还公开了苯并恶唑类化合物作为比例金属离子指示剂的用途。
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