公开(公告)号：US20160002737A1

公开(公告)日：2016-01-07

申请号：US14794488

申请日：2015-07-08

申请人： Martin Fuchs ,  Ravi Kapur ,  Mehmet Toner ,  Zihua Wang

发明人： Martin Fuchs ,  Ravi Kapur ,  Mehmet Toner ,  Zihua Wang

IPC分类号： C12Q1/68 ,  G01N1/40

CPC分类号： C12Q1/6886 ,  C12Q2600/156 ,  G01N1/405 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/57484 ,  G01N2015/1006 ,  G01N2015/1087 ,  G01N2800/385

摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g., epithelial cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject by analyzing a cellular sample from the subject.

摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的稀有细胞例如上皮细胞的方法。 本发明进一步具有分析稀有细胞以通过分析来自受试者的细胞样品来确定受试者中异常，疾病或病症的存在的方法。

公开(公告)号：US08784012B2

公开(公告)日：2014-07-22

申请号：US13480862

申请日：2012-05-25

申请人： Mehmet Toner ,  Dino DiCarlo ,  Jon F. Edd ,  Daniel Irimia

发明人： Mehmet Toner ,  Dino DiCarlo ,  Jon F. Edd ,  Daniel Irimia

IPC分类号： B65G53/52

CPC分类号： B01L3/502776 ,  B01D21/0087 ,  B01D21/265 ,  B01D45/04 ,  B01D45/12 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0883 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0433 ,  B01L2400/0454 ,  B01L2400/0487 ,  B01L2400/0622 ,  B01L2400/084 ,  B33Y80/00 ,  C12M23/16 ,  C12M41/36 ,  C12M47/04 ,  F16L41/02 ,  F17D1/00 ,  G01N15/0255 ,  G01N15/10 ,  G01N2015/0288 ,  G01N2015/1006 ,  G01N2015/1081 ,  Y10T137/85938 ,  Y10T137/85978 ,  Y10T436/117497 ,  Y10T436/2575

摘要： Various systems, methods, and devices are provided for focusing particles suspended within a moving fluid into one or more localized stream lines. The system can include a substrate and at least one channel provided on the substrate having an inlet and an outlet. The system can further include a fluid moving along the channel in a laminar flow having suspended particles and a pumping element driving the laminar flow of the fluid. The fluid, the channel, and the pumping element can be configured to cause inertial forces to act on the particles and to focus the particles into one or more stream lines.

摘要翻译： 提供了各种系统，方法和装置，用于将悬浮在移动流体中的颗粒聚焦到一个或多个局部流线中。 该系统可以包括基底和设置在基底上的至少一个通道，其具有入口和出口。 该系统还可以包括在具有悬浮颗粒的层流中沿着通道移动的流体和驱动流体层流的泵送元件。 流体，通道和泵送元件可以被配置成使惯性力作用在颗粒上并将颗粒聚焦成一条或多条流线。

公开(公告)号：US20140030231A1

公开(公告)日：2014-01-30

申请号：US13695459

申请日：2011-05-04

申请人： Martin L. Yarmush ,  Mehmet Toner ,  Maria-Louisa Izamis ,  Timothy Antonie Berendsen ,  Robert Marius Bieganski ,  Osman Berk Usta ,  Basak Elif Uygun ,  Mustafa Korkut Uygun ,  Sinem Perk

发明人： Martin L. Yarmush ,  Mehmet Toner ,  Maria-Louisa Izamis ,  Timothy Antonie Berendsen ,  Robert Marius Bieganski ,  Osman Berk Usta ,  Basak Elif Uygun ,  Mustafa Korkut Uygun ,  Sinem Perk

IPC分类号： A01N1/02

CPC分类号： A01N1/0226 ,  G01N1/42 ,  G01N33/5091

摘要： The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

摘要翻译： 本发明一般涉及用于确定用于移植和其它医疗目的的器官的活力的方法和组合物。 本发明的一个方面涉及一种通过测量能量参数来确定器官的能量水平来评估器官活力的方法，该方法是通过确定存储的细胞能量（例如，ATP水平）和/或特定的能量消耗 生存时间段 能量参数可以与参考能量参数进行比较，作为对活细胞产量和器官存活力的高度准确和可靠的预测。 本发明的另一方面涉及保存或延长器官存活时间的方法，任何组合（i）器官的保留灌注以预防缺血性损伤，（ii）器官的化学代谢抑制，例如使用代谢抑制剂， （iii）物理或环境条件下的代谢抑制，例如零度非冻结储存。

公开(公告)号：US20130189688A1

公开(公告)日：2013-07-25

申请号：US13835926

申请日：2013-03-15

申请人： Daniel Shoemaker ,  Ravi Kapur ,  Mehmet Toner ,  Roland Stoughton ,  Ronald W. Davis

发明人： Daniel Shoemaker ,  Ravi Kapur ,  Mehmet Toner ,  Roland Stoughton ,  Ronald W. Davis

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  B01L3/502761 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N2015/1006 ,  G01N2015/1087 ,  Y10T436/143333

摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

公开(公告)号：US08372584B2

公开(公告)日：2013-02-12

申请号：US11763421

申请日：2007-06-14

申请人： Daniel Shoemaker ,  Mehmet Toner ,  Ravi Kapur ,  Roland Stoughton

发明人： Daniel Shoemaker ,  Mehmet Toner ,  Ravi Kapur ,  Roland Stoughton

IPC分类号： C12Q1/68 ,  G01N33/48

CPC分类号： C12Q1/6881 ,  B01L3/502761 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N2015/1006 ,  G01N2015/1087 ,  Y10T436/143333

摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

公开(公告)号：US08304230B2

公开(公告)日：2012-11-06

申请号：US11726230

申请日：2007-03-21

申请人： Mehmet Toner ,  George Truskey ,  Ravi Kapur

发明人： Mehmet Toner ,  George Truskey ,  Ravi Kapur

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： B01L3/502753 ,  B01L3/502746 ,  B01L3/502761 ,  B01L9/54 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0877 ,  B01L2300/0883 ,  B01L2300/12 ,  B01L2400/0487 ,  B01L2400/086 ,  B33Y70/00 ,  B33Y80/00 ,  B82Y30/00 ,  C12N5/0087 ,  G01N1/40 ,  G01N1/405 ,  G01N33/5091 ,  G01N33/54366 ,  G01N33/54386 ,  G01N33/56966 ,  G01N33/574 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2333/70582 ,  G01N2333/70589 ,  G01N2333/70596 ,  Y10T29/49982 ,  Y10T428/24744

摘要： The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal blood). The method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).

摘要翻译： 本发明的特征在于从样品中分离细胞的方法（例如，将胎儿红细胞与母体血液分离）。 该方法开始于将包含细胞的样品引入一个或多个微流体通道。 在一个实施例中，该装置包括至少两个处理步骤。 例如，将细胞混合物引入微流体通道中，该微流体通道选择性地允许期望类型的细胞通过，然后将富含所需类型的细胞群引入第二微流体通道，其允许所需的 细胞以产生进一步富集所需类型的细胞群体。 细胞的选择基于混合物中细胞的性质，例如，大小，形状，变形性，表面特征（例如，细胞表面受体或抗原和膜通透性）或细胞内特性（例如，特定的表达 酶）。

公开(公告)号：US20120208186A1

公开(公告)日：2012-08-16

申请号：US13306520

申请日：2011-11-29

申请人： Ravi Kapur ,  Mehmet Toner ,  Zihua Wang ,  Martin Fuchs

发明人： Ravi Kapur ,  Mehmet Toner ,  Zihua Wang ,  Martin Fuchs

IPC分类号： G01N27/62

CPC分类号： C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N1/30 ,  G01N33/6893 ,  G01N2800/385

摘要： The present invention relates to methods for detecting, enriching, and analyzing rare cells that are present in the blood, e.g. fetal cells. The invention further features methods of analyzing rare cell(s) to determine the presence of an abnormality, disease or condition in a subject, e.g. a fetus by analyzing a cellular sample from the subject.

摘要翻译： 本发明涉及用于检测，富集和分析存在于血液中的罕见细胞的方法，例如， 胎儿细胞。 本发明还涉及分析稀有细胞以确定受试者中异常，疾病或病症的存在的方法，例如， 通过分析来自受试者的细胞样品的胎儿。

公开(公告)号：US20120171666A1

公开(公告)日：2012-07-05

申请号：US13306640

申请日：2011-11-29

申请人： Daniel Shoemaker ,  Martin Fuchs ,  Neil X. Krueger ,  Mehmet Toner ,  Darren Gray ,  Ravl Kapur ,  Zihua Wang

发明人： Daniel Shoemaker ,  Martin Fuchs ,  Neil X. Krueger ,  Mehmet Toner ,  Darren Gray ,  Ravl Kapur ,  Zihua Wang

IPC分类号： G01N27/62

CPC分类号： C12Q1/6883 ,  B01L3/502761 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N2015/1006 ,  G01N2015/1087 ,  Y10T436/143333

摘要： The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

公开(公告)号：US08043846B2

公开(公告)日：2011-10-25

申请号：US10560661

申请日：2004-06-09

申请人： Daniel Irimia ,  Mehmet Toner

发明人： Daniel Irimia ,  Mehmet Toner

IPC分类号： C12M1/34 ,  B01L3/00

CPC分类号： B01L3/502746 ,  B01F13/0066 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/046 ,  B01L2400/0487 ,  B01L2400/0688 ,  B33Y80/00 ,  C12M47/06 ,  Y10S366/02 ,  Y10S366/03 ,  Y10T436/118339

摘要： The invention features devices for mixing fluids, e.g., for lysing cells, and methods of use thereof. One device is based on the ability to control the flow of fluids, e.g., by contact angle and channel size. Fluids in this device can be divided to form segments of controlled volume, which are then brought together to initiate mixing. An exemplary use of the device is for the lysis of single cells. Another device is based on the ability to two mix two fluids in a channel and affinity capture of analytes. The devices can be integrated on the same chip with other devices, for example, for cell handling or analysis of DNA, mRNA, and proteins released from the lysis of a cell.

摘要翻译： 本发明的特征在于用于混合流体的装置，例如用于裂解细胞的装置及其使用方法。 一种装置基于例如通过接触角和通道尺寸来控制流体流动的能力。 该装置中的流体可以分成形成受控体积的区段，然后将其汇集在一起​​以开始混合。 该装置的示例性用途是用于单细胞的裂解。 另一种装置是基于两种混合通道中的两种流体和分析物的亲和性捕获的能力。 这些器件可以与其他器件集成在同一芯片上，例如用于细胞处理或分析从细胞裂解释放的DNA，mRNA和蛋白质。

公开(公告)号：US20100092393A1

公开(公告)日：2010-04-15

申请号：US12578303

申请日：2009-10-13

申请人： Ramin Haghgooie ,  Patrick Seamus Doyle ,  Mehmet Toner ,  Daniel Colin Pregibon

发明人： Ramin Haghgooie ,  Patrick Seamus Doyle ,  Mehmet Toner ,  Daniel Colin Pregibon

IPC分类号： A61K9/50 ,  C08J3/28 ,  C09K11/02 ,  B32B5/16 ,  C09D7/00 ,  A61K49/00 ,  G01N33/566

CPC分类号： C08J3/075 ,  A61K47/68 ,  A61K47/6921 ,  C08J2371/02 ,  C08L2205/14 ,  C09D7/20 ,  C09D7/70 ,  Y10T428/2982

摘要： Techniques are provided to produce and use non-spherical colloidal particles with independently tuned size, shape, flexibility, and chemical properties. A pre-polymer mixture for forming hydrogel particles includes a percentage of PEGDA selected to impart a target stiffness to the particles and includes, a percentage of acrylic acid selected to impart an independent target chemical function to the particles. The mixture also includes a percentage of photo-initiator to polymerize PEGDA upon exposure to a light source to impart an independently selected target size or shape or both to the particles.

摘要翻译： 提供技术来生产和使用具有独立调整的尺寸，形状，柔性和化学性质的非球形胶体颗粒。 用于形成水凝胶颗粒的预聚物混合物包括选择用于赋予颗粒目标硬度的百分比的PEGDA，并且包括一定百分比的丙烯酸，其被选择以赋予颗粒独立的目标化学功能。 该混合物还包括一定百分比的光引发剂，以在暴露于光源时聚合PEGDA以赋予颗粒独立选择的靶尺寸或形状或两者。