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177 条记录 (耗时 0.063 秒)

    Site-Specific Incorporation of Redox Active Amino Acids into Proteins
    42.
    发明申请
    Site-Specific Incorporation of Redox Active Amino Acids into Proteins 审中-公开
    将氧化还原活性氨基酸与位点特异性结合到蛋白质中

    公开(公告)号：US20120100570A1

    公开(公告)日：2012-04-26

    申请号：US13344436

    申请日：2012-01-05

    申请人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    发明人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    IPC分类号： C12Q1/25 ,  C12N9/00

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

    摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。

    Site-specific incorporation of redox active amino acids into proteins
    43.
    发明授权
    Site-specific incorporation of redox active amino acids into proteins 有权
    将氧化还原活性氨基酸的位点特异性掺入蛋白质

    公开(公告)号：US08114654B2

    公开(公告)日：2012-02-14

    申请号：US12806751

    申请日：2010-08-20

    申请人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    发明人： Lital Alfonta ,  Peter G. Schultz ,  Zhiwen Zhang

    IPC分类号： C12Q1/68 ,  C12N9/00 ,  C12N1/20

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

    摘要翻译： 提供了将氧化还原活性氨基酸掺入蛋白质的蛋白质生物合成机理组分的组成和方法，其中包括正交tRNA，正交氨酰-tRNA合成酶和tRNA /合成酶正交对。 还提供了用于鉴定这些正交对的方法以及使用这些正交对产生具有氧化还原活性氨基酸的蛋白质的方法。

    Genetically programmed expression of selectively sulfated proteins in eubacteria
    44.
    发明授权
    Genetically programmed expression of selectively sulfated proteins in eubacteria 有权
    细菌中选择性硫酸化蛋白质的遗传程序表达

    公开(公告)号：US08114627B2

    公开(公告)日：2012-02-14

    申请号：US11903499

    申请日：2007-09-20

    申请人： Chang C. Liu ,  Peter G. Schultz

    发明人： Chang C. Liu ,  Peter G. Schultz

    IPC分类号： C12N1/20 ,  C12N9/00 ,  C12N9/10

    CPC分类号： C12N9/93 ,  C07K14/815 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸磺基酪氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶，编码新合成酶分子的多核苷酸，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸磺酰酪氨酸的蛋白质的翻译方法和翻译系统。

    Genetic incorporation of unnatural amino acids into proteins in mammalian cells
    48.
    发明申请
    Genetic incorporation of unnatural amino acids into proteins in mammalian cells 有权
    在哺乳动物细胞中将非天然氨基酸遗传并入蛋白质中

    公开(公告)号：US20100021963A1

    公开(公告)日：2010-01-28

    申请号：US12311545

    申请日：2007-10-17

    申请人： Wenshe Liu ,  Peter G. Schultz

    发明人： Wenshe Liu ,  Peter G. Schultz

    IPC分类号： C12P21/00 ,  C12N5/071 ,  C12N5/10

    CPC分类号： C12N9/93 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins in mammalian host cells, for example, primate host cells and rodent host cells. The invention provides, for example but not limited to, translation systems that include host cells (e.g., primate or rodent cells), orthogonal aminoacyl-tRNA synthetases derived from eubacterial synthetases, orthogonal tRNAs, and the unnatural amino acid. The invention also relates to methods for producing proteins of interest comprising at least one unnatural amino acid in mammalian host cell systems.

    摘要翻译： 本发明涉及可将哺乳动物宿主细胞（例如灵长类动物宿主细胞和啮齿动物宿主细胞）中的蛋白质中含有非天然氨基酸的tRNA和氨酰-tRNA合成酶的正交对。 本发明提供了例如但不限于包含宿主细胞（例如灵长类或啮齿动物细胞），衍生自真细菌合成酶，正交tRNA和非天然氨基酸的正交氨酰-tRNA合成酶的翻译系统。 本发明还涉及用于在哺乳动物宿主细胞系统中产生包含至少一种非天然氨基酸的目的蛋白质的方法。

    Genetically programmed expression of selectively sulfated proteins in eubacteria
    49.
    发明申请
    Genetically programmed expression of selectively sulfated proteins in eubacteria 有权
    细菌中选择性硫酸化蛋白质的遗传程序表达

    公开(公告)号：US20090286279A1

    公开(公告)日：2009-11-19

    申请号：US12311169

    申请日：2007-09-20

    申请人： Chang C. Liu ,  Peter G. Schultz

    发明人： Chang C. Liu ,  Peter G. Schultz

    IPC分类号： C12P21/02 ,  C12N9/00 ,  C12N1/21 ,  C07K14/00

    CPC分类号： C12N9/93 ,  C07K14/815 ,  C12P21/02

    摘要： The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid sulfotyrosine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid sulfotyrosine and translation systems.

    摘要翻译： 本发明涉及tRNA和氨酰-tRNA合成酶的正交对，其可以将非天然氨基酸磺基酪氨酸掺入到在细菌宿主细胞如大肠杆菌中产生的蛋白质中。 本发明提供了例如但不限于新型正交氨酰-tRNA合成酶，编码新合成酶分子的多核苷酸，用于鉴定和制备新合成酶的方法，用于产生含有非天然氨基酸磺酰酪氨酸的蛋白质的翻译方法和翻译系统。