公开(公告)号：US20190324001A1

公开(公告)日：2019-10-24

申请号：US16459525

申请日：2019-07-01

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/18 ,  B01D15/22

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US10308690B2

公开(公告)日：2019-06-04

申请号：US14961164

申请日：2015-12-07

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Göran Baurén ,  Tomas Björkman

IPC: C07K14/31 ,  B01J20/32 ,  B01D15/38 ,  C07K1/22 ,  B01J20/286 ,  C07K14/745 ,  C07K16/00

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, comprising at least the mutation wherein the glutamine residue at position 9 has been mutated to a tryptophan, leucine, glutamic acid, valine or lysine. The invention also discloses multimers of the polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20180356375A1

公开(公告)日：2018-12-13

申请号：US16103149

申请日：2018-08-14

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: G01N30/60 ,  B01D15/22 ,  B01D15/18

CPC classification number: G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

Abstract: The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US10112980B2

公开(公告)日：2018-10-30

申请号：US15598685

申请日：2017-05-18

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Mats Ander ,  Goran Bauren ,  Tomas Bjorkman ,  Gustav Rodrigo

IPC: C07K14/31 ,  C07K14/745 ,  C07K16/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/32 ,  B01J20/286

Abstract: The invention discloses a polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A (SpA), as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamic acid residue at position 15 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.

公开(公告)号：US20180229152A1

公开(公告)日：2018-08-16

申请号：US15954545

申请日：2018-04-16

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer ,  Manoj Kumar Ramakrishna ,  Daniel Salomonsson ,  Per Uselius

IPC: B01D15/22 ,  B01D15/18 ,  G01N30/46 ,  G01N30/60 ,  F16L55/00

CPC classification number: B01D15/22 ,  B01D15/1864 ,  B01D15/1885 ,  F16L55/00 ,  G01N30/461 ,  G01N30/466 ,  G01N30/6004 ,  Y10T29/49826

Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b) connected in a rigid housing (21; 61), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules in the rigid housing are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US10041050B2

公开(公告)日：2018-08-07

申请号：US15355675

申请日：2016-11-18

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Patrik Adielsson ,  Tobias E. Soderman ,  Anna Akerblom

IPC: B01D15/38 ,  C12N7/00 ,  C12N7/02

Abstract: The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%.

公开(公告)号：US20180127460A1

公开(公告)日：2018-05-10

申请号：US15564232

申请日：2016-04-04

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01D15/36 ,  B01D15/38 ,  B01D15/34 ,  C12N9/64 ,  C07K14/755 ,  C07K16/00 ,  C07K14/765 ,  C07K14/47 ,  B01J41/20

CPC classification number: C07K1/18 ,  A61K35/16 ,  A61K38/00 ,  A61K38/36 ,  A61K38/4846 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  B01J41/20 ,  C07K14/472 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  C12N9/6424 ,  C12N9/644 ,  C12Y304/21022

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US09950277B2

公开(公告)日：2018-04-24

申请号：US14290357

申请日：2014-05-29

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Klaus Gebauer

IPC: B01D15/14 ,  G01N30/60 ,  G01N30/46 ,  B01D15/18 ,  B01D15/22 ,  G01N30/88

CPC classification number: B01D15/14 ,  B01D15/1864 ,  B01D15/1885 ,  B01D15/22 ,  G01N30/466 ,  G01N30/6039 ,  G01N30/6043 ,  G01N30/6091 ,  G01N2030/8881 ,  Y10T29/49826

Abstract: A parallel assembly (2; 11; 51) of chromatography column modules (3a,b,c; 13a,b,c; 53a,b,c, 90a, b), the assembly having one common assembly inlet (15; 55) and one common assembly outlet (17; 57), each column module comprising a bed space (29) filled with chromatography medium and each column module comprises integrated fluid conduits which when the column module is connected with other column modules are adapted to connect the bed space (29) of the column module with the assembly inlet (15; 55) and the assembly outlet (17; 57), wherein the total length and/or volume of the fluid conduit from the assembly inlet to one bed space together with the length and/or volume of the fluid conduit from the same bed space to the assembly outlet is substantially the same for all bed spaces and modules installed in the parallel assembly.

公开(公告)号：US09944722B2

公开(公告)日：2018-04-17

申请号：US13701074

申请日：2011-05-30

Applicant: Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

Inventor： Mattias Algotsson ,  Lars C. Andersson ,  Eggert Brekkan ,  Lee Hedemyr ,  Jean-Luc Maloisel ,  Ronnie Palmgren

IPC: C07C237/10 ,  C08B37/00 ,  B01J20/289 ,  B01J20/32 ,  C07C323/60 ,  B01J20/281

CPC classification number: C08B37/0039 ,  B01J20/281 ,  B01J20/289 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3265 ,  C07C237/10 ,  C07C323/60

Abstract: The present invention relates to dimeric pentadentate chelators with exceptionally strong binding of metal ions, for detection, immobilization and purification of biomolecules. Dimeric chelators offer a cooperativity of binding of two adjacent immobilized metal ions simultaneously to a histidine-tagged biomolecule, which gives advantageous properties regarding strength of binding compared to a corresponding monomer chelator. In addition, a dimer increases the selectivity (ease of separation) against non-tagged biomolecules with low metal-ion affinity.The dimeric pentadentate chelator according to the invention has the following general formula: wherein Sc is a scaffold or a connecting structure that contains at least two functional groups enabling coupling of two pentadentate chelators (PD), and PD is a pentadentate chelator having the formula:

公开(公告)号：US09878266B2

公开(公告)日：2018-01-30

申请号：US14542816

申请日：2014-11-17

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J Johansson ,  Jean-Luc Maloisel

IPC: C07K1/14 ,  B01D15/38 ,  B01D15/32 ,  B01D15/36 ,  B01J20/286 ,  B01J20/32 ,  B01J41/20 ,  C07K1/36 ,  C07K16/06 ,  C07K1/16 ,  C07K16/00

CPC classification number: B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.