公开(公告)号：US10525467B2

公开(公告)日：2020-01-07

申请号：US15342914

申请日：2016-11-03

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar EL-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: B01L3/00 ,  B01L7/00 ,  G01N27/447 ,  B01F13/00 ,  B01F13/08 ,  G01N35/00

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

公开(公告)号：US10210410B2

公开(公告)日：2019-02-19

申请号：US14919741

申请日：2015-10-22

Applicant: IntegenX Inc.

Inventor： Robert A. Schueren ,  David King ,  Chungsoo Charles Park ,  Stevan B. Jovanovich

IPC: G06K9/00 ,  G10L17/00 ,  G06F19/10 ,  G16H40/63 ,  G16H10/40

Abstract: A biometric biochemical analysis system includes a user interface module to provide instructions for collecting and handling biochemical sampling and processing related to biometric data gathering as well as capturing biometric data using digital data capturing devices. The user interface module and display are integrated with analysis and communications portions of the biometric biochemical analysis system to provide a portable system for multi-portion data collecting, storage, verification, and analysis.

公开(公告)号：US10191071B2

公开(公告)日：2019-01-29

申请号：US15037039

申请日：2014-11-17

Applicant: IntegenX Inc.

Inventor： James Ogg ,  David Eberhart ,  William D. Nielsen ,  Helen Franklin ,  Stevan B. Jovanovich

IPC: G01N35/00 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6888

Abstract: Provided herein are instruments and cartridges for processing samples. The cartridges include fluidic circuits in which fluid movement can be regulated by diaphragm valves. In certain cartridges, deformable material providing a diaphragm contacts an interface in the instrument that actuates the diaphragm directly, without intervening actuation layer. Certain cartridges have a plurality of fluidic circuits and fluid distribution channels or pneumatic distribution channels configured to deliver fluids or pneumatic pressure to any of the fluidic circuits, selectively. Certain cartridges have compartments containing on-board reagents. Compartments can be closed by a film attached to a body the cartridge through a heat seal.

公开(公告)号：US09341284B2

公开(公告)日：2016-05-17

申请号：US14320384

申请日：2014-06-30

Applicant: IntegenX Inc.

Inventor： Mattias Vangbo

IPC: F16K31/00 ,  F16K99/00 ,  B01L3/00 ,  F04B19/00 ,  F04B43/04

CPC classification number: F16K99/0015 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2400/0638 ,  B01L2400/0655 ,  B33Y70/00 ,  B33Y80/00 ,  F04B19/006 ,  F04B43/043 ,  F16K99/0055 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0324 ,  Y10T137/0402 ,  Y10T137/7895

Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.

Abstract translation: 本发明提供了一种流体装置，其包括具有流体层，致动层和在流体层和致动层之间的弹性层的隔膜阀，弹性层具有通过以下方式机械地密封流体层和致动层的隔膜 致动层中的密封环。

公开(公告)号：US20160016140A1

公开(公告)日：2016-01-21

申请号：US14804675

申请日：2015-07-21

Applicant: Stevan B. Jovanovich ,  Seth Stern ,  Ezra Van Gelder ,  Mattias Vangbo

Inventor： Stevan B. Jovanovich ,  Seth Stern ,  Ezra Van Gelder ,  Mattias Vangbo

IPC: B01J19/00 ,  C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00389 ,  B01J2219/00599 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0084

Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

Abstract translation: 本发明提供用于执行综合分析的系统，设备，方法和套件。 综合分析可以包括样品处理，库构建，扩增和测序。 集成分析可以在一个或多个流体连接的模块中进行。 一个或多个模块可以由计算机控制和/或自动化。 可以对组织样品，临床样品或环境样品进行综合分析。 集成分析系统可以具有紧凑的格式，并在指定的时间段内返回结果。

公开(公告)号：US20140370519A1

公开(公告)日：2014-12-18

申请号：US14253622

申请日：2014-04-15

Applicant: IntegenX Inc.

Inventor： Mattias Vangbo ,  William D. Nielsen ,  Iuliu Iouan Blaga ,  Seth Stern ,  Joanne Horn ,  Michael Van Nguyen ,  Stevan Jovanovich

IPC: G01N1/34

CPC classification number: G01N1/34 ,  B01D17/06 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0819 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/043 ,  G01N1/40 ,  G01N27/44791 ,  G01N35/1095 ,  G01N2035/00158 ,  Y10T436/143333 ,  Y10T436/25375

Abstract: The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

Abstract translation: 本发明提供用于将微芯片连接到墨盒和气动歧管的装置和方法。 墨盒，微芯片和气动歧管可以与下游制备装置（如热调节装置和分离和分析装置）集成。

公开(公告)号：US08894946B2

公开(公告)日：2014-11-25

申请号：US13656503

申请日：2012-10-19

Applicant: IntegenX Inc.

Inventor： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar El-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC: G01N21/75 ,  C07H21/00 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/68 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统。 该系统可以执行多个操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。

公开(公告)号：US20140065689A1

公开(公告)日：2014-03-06

申请号：US13795324

申请日：2013-03-12

Applicant: IntegenX Inc.

Inventor： Michael Hogan ,  James C. Davis

IPC: A01N1/02

CPC classification number: A01N1/0263 ,  A61K38/02 ,  C07K1/042 ,  C07K1/047 ,  C07K17/00 ,  C07K17/08 ,  C12N15/1006 ,  G01N33/543 ,  G01N33/68 ,  G01N33/6842 ,  Y10T436/2525

Abstract: The invention provides compositions and methods for storage of biomolecules. The biomolecules are stored via absorption to a substrate. Absorbed biomolecules can be eluted or recovered from the substrate at a future time, and optionally be subjected to a subsequent analysis or application. Biomolecules absorbed to a substrate for storage may also optionally be preserved, i.e., the absorbed biomolecule is resistant to or resists degradation.

Abstract translation: 本发明提供用于储存生物分子的组合物和方法。 通过吸收将生物分子储存到底物中。 吸收的生物分子可以在将来的时间从底物洗脱或回收，并且任选地进行随后的分析或应用。 吸收到用于储存的底物的生物分子也可以任选地被保留，即，所吸收的生物分子对抗或抵抗降解。

公开(公告)号：US08512538B2

公开(公告)日：2013-08-20

申请号：US13113968

申请日：2011-05-23

Applicant: Lars Majlof ,  Ezra Van Gelder

Inventor： Lars Majlof ,  Ezra Van Gelder

IPC: G05D23/00 ,  G01N27/447 ,  G01N27/453

CPC classification number: G01N27/44708

Abstract: This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries.

Abstract translation: 本发明提供一种毛细管电泳装置，其中毛细管在附着于电绝缘电路板的热响应电路上进行热调节。 本发明还提供了一种用于扫描毛细管阵列的光学扫描仪。 激光器，光学检测器和光学选择器处于允许光学检测器选择性地检测来自多个电泳毛细管中的一个或多个的光学信号的布置。

公开(公告)号：US08431390B2

公开(公告)日：2013-04-30

申请号：US13287398

申请日：2011-11-02

Applicant: Stevan Bogdan Jovanovich ,  Iuliu Ioan Blaga

Inventor： Stevan Bogdan Jovanovich ,  Iuliu Ioan Blaga

IPC: C12P19/34 ,  C12N13/00 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00 ,  C12M1/02

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0059 ,  B01J2219/005 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0654 ,  B01L2300/0803 ,  B01L2300/0806 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2400/0409 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/086 ,  C12Q2565/629 ,  G01N1/34 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44791 ,  G01N35/0098

Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.

Abstract translation: 公开了用于将微芯片连接到各种类型的模块的方法和装置。 所公开的技术可用作各种应用的样品制备和分析系统，如DNA测序和基因分型，蛋白质组学，病原体检测，诊断和生物防治。