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    METHOD FOR SEPARATING ALPHA-LACTALBUMIN AND BETA-LACTOGLOBULIN
    42.
    发明申请
    METHOD FOR SEPARATING ALPHA-LACTALBUMIN AND BETA-LACTOGLOBULIN 审中-公开

    公开(公告)号：US20170318835A1

    公开(公告)日：2017-11-09

    申请号：US15503924

    申请日：2015-08-14

    Applicant: Upfront Chromatography A/S

    Inventor： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    IPC: A23J1/20 ,  C07K14/47 ,  C07K1/18 ,  C07K14/76 ,  A23C9/146

    CPC classification number: A23J1/205 ,  A23C9/1465 ,  C07K1/18 ,  C07K14/4717 ,  C07K14/76

    Abstract: The present invention relates to a method for providing an alpha-lactalbumin fraction and a beta-lactoglobulin fraction from a whey material obtained from milk, the method comprising the steps of: (i) providing the whey material; (ii) contacting the whey material with a chromatographic support allowing beta-lactoglobulin to be retained by the chromatographic support; (iii) obtaining a permeate fraction from the chromatographic support comprising the alpha-lactalbumin fraction; (iv) optionally washing the chromatographic support; and (v) obtaining a retentate fraction from the chromatographic support comprising the beta-lactoglobulin fraction; wherein the whey material provided in step (i) has been depleted, or substantially depleted from at least one whey protein, such as at least 2 whey proteins, e.g. at least 3 whey proteins.

    PROCESS FOR GENERATION OF PROTEIN AND USES THEREOF
    47.
    发明申请
    PROCESS FOR GENERATION OF PROTEIN AND USES THEREOF 审中-公开

    公开(公告)号：US20170152499A1

    公开(公告)日：2017-06-01

    申请号：US15424513

    申请日：2017-02-03

    Applicant: The Australian National University

    Inventor： Jill E. GREADY ,  Babu KANNAPPAN

    IPC: C12N9/88

    CPC classification number: C12N9/88 ,  C07K1/00 ,  C07K1/13 ,  C07K1/16 ,  C07K1/18 ,  C07K1/36 ,  C07K14/415 ,  C07K2299/00 ,  C12N15/1089 ,  C12Y401/01039

    Abstract: A method of generating a protein with an improved functional property, the method comprising: (a) identifying at least one Target amino acid Residue in a first protein, wherein said Target amino acid Residue is associated with said functional property; (b) comparing at least one homologous second protein from the same or a different phylogenetic branch as the first protein with the first protein and identifying at least one Variant amino acid Residue between the first protein and the second protein; (c) selecting at least one Candidate amino acid Residue from the Variant amino acid Residue identified in (b) on the basis of said Candidate amino acid Residue affecting said Target amino acid Residue with respect to said functional property; (d) forming at least one Candidate Mutant protein in silico or producing at least one Candidate Mutant protein in vitro in which said at least one Candidate amino acid Residue from the second protein substitutes a corresponding residue in the first protein; and (e) screening said at least one Candidate Mutant protein produced in (d) to identify a protein having said improved functional property; a Rubisco protein and its large subunit polypeptide produced by the method; and polynucleotides encoding the same.

    METHOD OF PURIFYING THERAPEUTIC PROTEINS
    49.
    发明申请
    METHOD OF PURIFYING THERAPEUTIC PROTEINS 审中-公开

    公开(公告)号：US20170143805A1

    公开(公告)日：2017-05-25

    申请号：US15424072

    申请日：2017-02-03

    Applicant: CSL Behring GmbH

    Inventor： Hung PHAM ,  Jeffrey Michael HEY ,  Darren NGUY

    IPC: A61K38/36

    CPC classification number: B01D15/363 ,  A61J1/05 ,  A61K38/00 ,  A61K38/36 ,  A61K38/363 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K14/745 ,  C07K14/75 ,  C07K14/755

    Abstract: The present invention relates generally to a method of reducing the level of at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in a solution comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and von Willebrand factor (VWF), the method comprising: (i) passing a feedstock comprising at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF through a hydrophobic charge-induction chromatographic resin under conditions selected such that at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) present in the feedstock is bound to the resin; and (ii) recovering a solution comprising the at least one protein selected from the group consisting of fibrinogen, Factor VIII and VWF which passes through the resin, wherein the concentration of the at least one protein selected from the group consisting of plasminogen, tissue plasminogen activator and other protease(s) in the solution is reduced by at least 50% compared to the feedstock. Also provided are solutions and pharmaceutical formulations comprising the fibrinogen and/or Factor VIII and/or VWF recovered by such methods, and uses thereof.

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