公开(公告)号：US20240254419A1

公开(公告)日：2024-08-01

申请号：US18400922

申请日：2023-12-29

申请人： UNIVERCELLS TECHNOLOGIES S.A.

发明人： José Castillo ,  Bastien Mairesse ,  Sebastien Jean-Pierre Michel Rodriguez

IPC分类号： C12M1/00 ,  C12M1/02 ,  C12M1/12 ,  C12M3/00

CPC分类号： C12M23/34 ,  C12M23/42 ,  C12M23/44 ,  C12M25/18 ,  C12M27/00 ,  C12M27/18

摘要： An apparatus for culturing cells includes a bioreactor. The bioreactor may be modular and may include in a chamber a fixed bed, such as an unstructured or structured fixed bed (such as a spiral bed) for culturing cells, with a return column arranged centrally within the chamber. The modular bioreactor may include a plurality of structured fixed bed arranged in a stacked configuration. The modular bioreactor may include an outer casing forming a space for conditioning (e.g., insulating, heating, cooling) at least a chamber in which cells are cultured. The bioreactor may also include an impeller with radially curved blades, and may also suspend the impeller so that it may move from side-to-side and align with an external drive. Related methods are also disclosed.

公开(公告)号：US20230348834A1

公开(公告)日：2023-11-02

申请号：US17779044

申请日：2020-10-30

申请人： CORNING INCORPORATED

发明人： Sumitava De ,  Abhijit Rao ,  Ryann Loren Russell ,  Yujian Sun

IPC分类号： C12M1/12 ,  C12M1/08

CPC分类号： C12M25/18 ,  C12M27/24

摘要： A packed-bed bioreactor is provided that includes: a vessel having an interior cavity defined by an outer wall; and a center column disposed within the interior cavity. The center column includes a columnar sidewall defining an inner region within the center column, the columnar sidewall separating the inner region from an outer region within the interior cavity. The bioreactor further includes a cell culture substrate disposed in the outer region of the cavity, the cell culture substrate surrounding the center column; at least one port extending through the vessel for at least one supply and removal of media to or from the interior cavity; and a fountain head element disposed above the center column.

公开(公告)号：US11781102B2

公开(公告)日：2023-10-10

申请号：US15777228

申请日：2017-04-25

申请人： Trizell Ltd.

发明人： Hanna P. Lesch ,  Joonas Malinen ,  Eevi Lipponen ,  Anniina Valkama ,  Hanna Leinonen

IPC分类号： C12M1/00 ,  C12M1/02 ,  C12P21/02 ,  C12M1/12 ,  C12N15/86 ,  C12M1/34 ,  C07K14/005

CPC分类号： C12M29/18 ,  C07K14/005 ,  C12M1/02 ,  C12M25/18 ,  C12M29/10 ,  C12M41/26 ,  C12M41/46 ,  C12N15/86 ,  C12P21/02 ,  C12N2740/15051

摘要： We have found a way to make possible large-scale plasmid transfection using PEI to produce high titer viral vectors in fixed bed or adherent cell culture bioreactors by using PEI as a transfection agent, while avoiding formation of the PEI-plasmid precipitate which in prior art approaches clogged adherent bioreactor substrates. We have also found a way to improve PEI-based transfection by modifying how pH and CO2 are managed during transfection.

公开(公告)号：US20230203418A1

公开(公告)日：2023-06-29

申请号：US17928416

申请日：2021-05-31

申请人： UNIVERCELLS TECHNOLOGIES SA

发明人： Sarah Beck ,  Christophe Dumont ,  Thibault Poncelet ,  Alexandre Vanhaver ,  Jean-Christophe Drugmand ,  Andy Reniers ,  Laetitia De Viron

IPC分类号： C12M1/00 ,  C12M1/12

CPC分类号： C12M23/52 ,  C12M25/18 ,  C12M23/34

摘要： The current invention relates to a bioreactor cabinet, configured to be incorporated in a biomolecule production system, wherein said bioreactor cabinet is preferably a mobile bioreactor cabinet suited to receive a bioreactor, said bioreactor cabinet is provided with a bioreactor docking station, wherein said bioreactor cabinet, more preferably a side wall of said bioreactor cabinet, is provided with a connector allowing the transmission of power, signals and/or data when paired with a biomolecule production system, such as a bioreactor chamber of said system. The current invention equally relates to a biomolecule production system and methods for producing biomolecules.

公开(公告)号：US20180044622A1

公开(公告)日：2018-02-15

申请号：US15557616

申请日：2016-03-11

申请人： GeneHarbor (Hong Kong) Biotechnologies Limited

发明人： Wing Keung Eric Poon ,  Yau Lung Siu ,  Junming Chen ,  Dong Shen ,  Jun Wang ,  Liang Sun

IPC分类号： C12M1/40 ,  C12M1/34 ,  C12M1/12 ,  C12M1/00 ,  C12P19/40 ,  C12P13/00

CPC分类号： C12M21/18 ,  C12M1/00 ,  C12M1/40 ,  C12M25/00 ,  C12M25/18 ,  C12M29/00 ,  C12M29/10 ,  C12M41/26 ,  C12M41/40 ,  C12P1/00 ,  C12P1/04 ,  C12P13/00 ,  C12P13/001 ,  C12P19/40

摘要： The present invention provides an immobilized reaction device and a method for carrying out reaction by utilizing the immobilization technology. The immobilized reaction device includes a columnar reactor with an inlet and an outlet. The reactor is provided with an interior cavity which is defined by a top portion and a bottom portion opposite to each other, and a side wall connecting the top portion and the bottom portion.

公开(公告)号：US20170022465A1

公开(公告)日：2017-01-26

申请号：US15217347

申请日：2016-07-22

申请人： Timothy Ray Ho

发明人： Timothy Ray Ho

IPC分类号： C12M3/06 ,  C12M1/36 ,  C12M1/12 ,  C12M1/00

CPC分类号： C12M25/18 ,  C12M27/10 ,  C12M27/20 ,  C12M41/48

摘要： Disclosed herein is an improved rocked bioreactor system used for carrying out cell and tissue cultures.

摘要翻译： 本文公开了用于进行细胞和组织培养物的改进的摇动生物反应器系统。

公开(公告)号：US20160281045A1

公开(公告)日：2016-09-29

申请号：US15036948

申请日：2014-11-14

申请人： LOUGHBOROUGH UNIVERSITY

发明人： Mark MCCALL ,  Robert THOMAS

IPC分类号： C12M1/12 ,  C12M1/00 ,  C12N5/0775 ,  C12N5/00

CPC分类号： C12M25/18 ,  C12M23/34 ,  C12M23/40 ,  C12N5/0075 ,  C12N5/0663 ,  C12N5/0668 ,  C12N2533/10

摘要： The present invention provides methods, devices and systems of culturing cells in a cell culture5 device comprising a plurality of non-porous substrates in the form of a packed bed in which the addition of substrates to the cell culture device enables specific yields to be obtained. Modified non-porous substrates are also provided for use in such methods, devices and systems.

摘要翻译： 本发明提供了在细胞培养物5中装置培养细胞的方法，装置和系统，该装置包括填充床形式的多个无孔底物，其中向细胞培养装置添加底物使得能够获得特定的产率。 还提供了修改的无孔基材用于这些方法，装置和系统中。

公开(公告)号：US20160136333A1

公开(公告)日：2016-05-19

申请号：US14901108

申请日：2014-06-27

申请人： UNIVERSITÄT ZÜRICH

发明人： Ernst REICHMANN

IPC分类号： A61L27/60 ,  A61L27/38 ,  C12M1/00 ,  C12M1/12 ,  C12M1/24 ,  C12M3/00 ,  A61L27/52 ,  C12N5/071

CPC分类号： A61L27/60 ,  A61F2/105 ,  A61L27/3804 ,  A61L27/3813 ,  A61L27/52 ,  A61L2430/34 ,  C12M23/08 ,  C12M23/22 ,  C12M23/48 ,  C12M25/14 ,  C12M25/18 ,  C12N5/0629 ,  C12N2533/90 ,  C12N2537/00

摘要： The invention relates to a device and a method for compressing a hydrogel layer (H), wherein a separate piston (600) that is designed to be connected to a second component (400) of the device in a releasable manner in an operating mode of the device, particularly by means of a latching connection, presses along a compression direction (C) against said hydrogel layer (H) residing on a membrane bottom (501) of a graft frame (500) so as to compress the hydrogel layer (H) between the piston (600) and the membrane bottom (501).

摘要翻译： 本发明涉及一种用于压缩水凝胶层（H）的装置和方法，其中分离的活塞（600）被设计成以可释放的方式连接到装置的第二部件（400） 特别是通过闩锁连接装置，该装置沿着压缩方向（C）压靠在驻留在移植物框架（500）的膜底部（501）上的所述水凝胶层（H）上，以便压缩水凝胶层（H ）在活塞（600）和膜底（501）之间。

公开(公告)号：US20160096761A1

公开(公告)日：2016-04-07

申请号：US14731420

申请日：2015-06-05

申请人： J.S. Meyer Engineering, P.C.

发明人： Stanley Marcus MEYER ,  Jason Scott MEYER

IPC分类号： C02F11/04

CPC分类号： C02F11/04 ,  C02F1/722 ,  C02F3/2806 ,  C02F3/286 ,  C02F2103/005 ,  C02F2103/20 ,  C02F2203/00 ,  C02F2301/08 ,  C02F2301/106 ,  C02F2303/02 ,  C12M21/04 ,  C12M23/58 ,  C12M25/18 ,  C12M47/18

摘要： The present disclosure relates, according to some embodiments, to systems and methods for processing organic compounds, such as manure or other organic waste. Embodiments may comprise a first containment chamber, a first anaerobic chamber, and a second anaerobic chamber. A first anaerobic chamber may receive organic compounds from a first containment chamber, and may provide a fluid stream to a second anaerobic chamber. The second anaerobic chamber may comprise a substrate, such as lava rock, with bacteria growing thereon. Further, a sulfide gas treating unit may receive and treat sulfide gases from a first anaerobic chamber and/or a second anaerobic chamber. A water storage unit may receive and store waste water or effluent from a first anaerobic chamber and/or a second anaerobic chamber.

摘要翻译： 根据一些实施方案，本公开涉及用于处理有机化合物如粪便或其它有机废物的系统和方法。 实施例可以包括第一容纳室，第一厌氧室和第二厌氧室。 第一厌氧室可以从第一容纳室接收有机化合物，并且可以向第二厌氧室提供流体流。 第二厌氧室可以包括其上生长有细菌的基底，例如熔岩岩石。 此外，硫化物气体处理单元可以接收和处理来自第一厌氧室和/或第二厌氧室的硫化物气体。 储水单元可以接收并存储来自第一厌氧室和/或第二厌氧室的废水或流出物。

公开(公告)号：US09273278B2

公开(公告)日：2016-03-01

申请号：US14148631

申请日：2014-01-06

申请人： CESCO BIOENGINEERING CO., LTD.

发明人： Vincent Lee ,  Peter Chen ,  Audrey Chen ,  Alex Liu ,  Chungnan Fu ,  Gray Wang ,  Hugo Chen ,  King-Ming Chang ,  Lewis Ho

IPC分类号： C12N11/00 ,  C12M1/02 ,  C12M1/12 ,  C12M1/00

CPC分类号： C12M25/14 ,  C12M25/18 ,  C12M47/02

摘要： A large scale cell harvesting method for a pack-bed culture device by providing cell growing space that is packed with a plurality of porous matrices and/or matrix means. When a cell detaching step at least comprising shaking or spinning the culture chamber is performed, the unoccupied space is large enough for the porous matrices collide to each other or to the culture chamber to generate momentum whereby the cells detach from the porous matrices to harvest the cells.

摘要翻译： 通过提供填充有多个多孔基质和/或基质装置的细胞生长空间来进行包装床培养装置的大规模细胞收获方法。 当进行至少包括振荡或纺丝培养室的细胞分解步骤时，未占据的空间足够大以使多孔基质相互碰撞或与培养室相撞以产生动量，由此细胞从多孔基质分离以收获 细胞。