公开(公告)号：US20160289736A1

公开(公告)日：2016-10-06

申请号：US15034817

申请日：2014-11-05

申请人： BIOFIRE DIAGNOSTICS, LLC ,  UNIVERSITY OF UTAH RESEARCH FOUNDATION

发明人： David Jones ,  Carl T. Wittwer

IPC分类号： C12Q1/68 ,  C12Q3/00 ,  G01N35/00 ,  B01L7/00

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/147 ,  B01L2300/06 ,  B01L2300/0609 ,  B01L2300/0627 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2300/16 ,  B01L2300/1816 ,  B01L2300/185 ,  B01L2300/1894 ,  C12Q3/00 ,  G01N35/00623 ,  G01N35/00871 ,  G01N2035/00376 ,  G01N2035/00396 ,  G01N2035/00445

摘要： Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in

摘要翻译： 提供了方法，装置和试剂盒，用于使用感应加热在<20秒/周期内执行PCR和其他热循环反应。

公开(公告)号：US09452433B2

公开(公告)日：2016-09-27

申请号：US14290057

申请日：2014-05-29

申请人： Advanced Liquid Logic, Inc. ,  Duke University

发明人： Alexander Shenderov ,  Michael G. Pollack

IPC分类号： B01L7/00 ,  B01L3/00

CPC分类号： B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

摘要： Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.

公开(公告)号：US20160266111A1

公开(公告)日：2016-09-15

申请号：US15033319

申请日：2014-10-31

申请人： KONICA MINOLTA, INC.

发明人： Kosuke NAGAE ,  Hiroshi HIRAYAMA

IPC分类号： G01N33/543 ,  G01N1/14 ,  G01N33/553 ,  G01N21/64

CPC分类号： G01N33/54373 ,  B01L3/5027 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/1816 ,  G01N1/14 ,  G01N21/0332 ,  G01N21/13 ,  G01N21/553 ,  G01N21/6428 ,  G01N21/648 ,  G01N33/553 ,  G01N2021/6439 ,  G01N2201/0231 ,  G01N2201/06113 ,  G01N2201/064

摘要： This detection device has a holder and a heating unit. The holder holds a detection chip that has the following: a prism that has an incidence surface and a film-formation surface; a metal film formed on said film-formation surface; trapping bodies laid out on the surface of said metal film; and a substrate that is laid out on the surface of the metal film, and together with the metal film, forms a liquid collection section in which a liquid is collected. The heating unit heats at least one of the substrate, the prism, and the metal film either while in contact therewith or without contacting same. Also, the heating unit is positioned so as to avoid the path that excitation light takes from an excitation-light emission unit to the abovementioned incidence surface.

摘要翻译： 该检测装置具有支架和加热单元。 保持器保持具有以下的检测芯片：具有入射表面和成膜表面的棱镜; 形成在所述成膜表面上的金属膜; 在所述金属膜的表面上布置的捕获体; 以及布置在金属膜的表面上的基板，与金属膜一起形成收集液体的液体收集部。 加热单元在与其接触或不与其接触的同时加热基板，棱镜和金属膜中的至少一个。 此外，加热单元被定位成避免激发光从激发光发射单元到上述入射表面的路径。

公开(公告)号：US09404151B2

公开(公告)日：2016-08-02

申请号：US13695254

申请日：2011-04-28

申请人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/68 ,  B01L7/00 ,  C12Q3/00

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/1816 ,  C12Q3/00

摘要： The present disclosure provides a non-contact real time micro Polymerase Chain Reaction (PCR) system comprising; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising an associated LED driver and a photo detector amplifier placed above the chip to detect fluorescence; an induction heater mounted around the chip and inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein the infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.

摘要翻译： 本公开提供了非接触式实时微聚合酶链式反应（PCR）系统，其包含： 具有用于保持样品的反应室和用于加热样品的反应室下面的嵌入式金属加热器的芯片; 光学单元，包括相关联的LED驱动器和放置在芯片上方以检测荧光的光电检测放大器; 安装在芯片周围并感应耦合到金属加热器的感应加热器; 安装在所述芯片下方的用于测量所述金属加热器的温度的红外温度传感器，其中所述红外温度传感器与信号调节器接口; 以及与信号调节器和感应加热器接口的控制器，其基于通过信号调节器从红外温度传感器接收的反馈来调节对感应加热器的电力。

公开(公告)号：US20150224503A1

公开(公告)日：2015-08-13

申请号：US14423412

申请日：2013-08-23

申请人： Siemens Healthcare Diagnostics Inc.

发明人： Paul Dulaff

IPC分类号： B01L7/00 ,  H05B6/10

CPC分类号： B01L7/00 ,  B01L3/0275 ,  B01L2200/147 ,  B01L2300/0627 ,  B01L2300/1816 ,  G01N1/44 ,  G01N35/1004 ,  G01N2035/00425 ,  G01N2035/1048 ,  H05B6/108

摘要： A heater for heating fluidic elements and fluids is provided. The heater quickly and efficiently heats elements and samples without occupying a lot of space in in vitro diagnostic environments. The heater includes an induction coil, sized and configured to allow for a fluidic element to be placed therein, and induction circuitry coupled to the induction coil that facilitates induction heating through electromagnetic induction. A current is generated to pass through the induction coil, creating a field within the induction coil that generates heat that is transferrable to conductive objects placed within the field. In this manner, heat is transferred to the fluidic element and to fluids in contact with the fluidic element.

摘要翻译： 提供了用于加热流体元件和流体的加热器。 加热器快速有效地加热元件和样品，而不会在体外诊断环境中占据很大的空间。 加热器包括感应线圈，其尺寸和构造允许将流体元件放置在其中，以及耦合到感应线圈的感应电路，其促进通过电磁感应的感应加热。 产生电流通过感应线圈，在感应线圈内产生产生可转移到放置在场中的导电物体的热的场。 以这种方式，热量被传递到流体元件和与流体元件接触的流体。

公开(公告)号：US08864967B2

公开(公告)日：2014-10-21

申请号：US11631389

申请日：2005-06-30

申请人： Gilles Marchand ,  Yves Fouillet ,  Philippe Clementz

发明人： Gilles Marchand ,  Yves Fouillet ,  Philippe Clementz

IPC分类号： B01D59/42 ,  F04B19/00 ,  B01L3/00

CPC分类号： B01L3/502792 ,  B01L3/5025 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2400/0427 ,  F04B19/006 ,  Y10T137/206 ,  Y10T137/2185

摘要： A device for displacing a small volume of liquid under the effect of an electric control, including a first substrate with a hydrophobic surface provided with a first electrical conductor, a second electrical conductor positioned facing the first conductor, and a third conductor, forming with the second conductor, a mechanism for analyzing or heating a volume of liquid.

摘要翻译： 一种用于在电控制的作用下移动小体积的液体的装置，包括具有设置有第一电导体的疏水表面的第一基板，面对第一导体的第二电导体和与第一导体成形的第三导体 第二导体，用于分析或加热一定体积的液体的机构。

公开(公告)号：US20130101983A1

公开(公告)日：2013-04-25

申请号：US13695254

申请日：2011-04-28

申请人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

发明人： Swetha Chandra ,  Sudip Mondal ,  Venkataraman Venkatakrishnan ,  Sathyadeep Viswanathan ,  Renjith Mahiladevi Radhakrishnan ,  Raviprakash Jayaraman ,  Chandrasekhar Bhaskaran Nair ,  Pillarisetti Venkata Subbarao

IPC分类号： C12Q1/68 ,  C12Q3/00

CPC分类号： C12Q1/686 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0684 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2300/1816 ,  C12Q3/00

摘要： The present disclosure provides a non contact real time micro Polymerase Chain Reaction [PCR] system comprises; a chip having a reaction chamber for holding a sample and an embedded metal heater below the reaction chamber for heating the sample; an optical unit comprising associated LED driver and photo detector amplifier placed above the chip to detect the fluorescence; an induction heater mounted around the chip and is inductively coupled to the metal heater; an infrared temperature sensor mounted below the chip for measuring a temperature of the metal heater, wherein said infrared temperature sensor is interfaced with a signal conditioner; and a controller interfaced with the signal conditioner and the induction heater for regulating the power to the induction heater based on feedback received from the infrared temperature sensor through the signal conditioner.

摘要翻译： 本公开提供了非接触式实时微聚合酶链式反应[PCR]系统，包括： 具有用于保持样品的反应室和用于加热样品的反应室下面的嵌入式金属加热器的芯片; 光学单元，包括放置在芯片上方的相关联的LED驱动器和光电检测放大器，以检测荧光; 感应加热器，安装在芯片周围，并感应耦合到金属加热器; 安装在所述芯片下方的用于测量所述金属加热器的温度的红外温度传感器，其中所述红外温度传感器与信号调节器接口; 以及控制器，其与信号调节器和感应加热器接口，用于基于通过信号调节器从红外温度传感器接收的反馈来调节对感应加热器的电力。

公开(公告)号：US20120132528A1

公开(公告)日：2012-05-31

申请号：US13006798

申请日：2011-01-14

申请人： Alexander D. Shenderov ,  Michael G. Pollack

发明人： Alexander D. Shenderov ,  Michael G. Pollack

IPC分类号： G01N27/447 ,  H02K44/02

CPC分类号： B01L7/525 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/1816 ,  B01L2300/1827 ,  B01L2300/1872 ,  B01L2400/0427

摘要： The invention provides a method for dispensing liquid, comprising the steps of: (a) positioning a droplet to be dispensed in a gap of an electrowetting device using an electrowetting array; and (b) dispensing the droplet through a hole in a housing or substrate of the electrowetting device. The invention further provides a method for withdrawing liquid comprising the steps of: (a) positioning a droplet to be withdrawn from a gap of an electrowetting device using an electrowetting array; and (b) withdrawing the droplet through a hole in a housing or substrate of the electrowetting device.

摘要翻译： 本发明提供一种分配液体的方法，包括以下步骤：（a）使用电润湿阵列将要分配的液滴定位在电润湿装置的间隙中; 和（b）通过电润湿装置的壳体或基底中的孔分配液滴。 本发明还提供了一种用于抽出液体的方法，包括以下步骤：（a）使用电润湿阵列将要从电润湿装置的间隙取出的液滴定位; 和（b）通过电润湿装置的壳体或基底中的孔抽出液滴。

公开(公告)号：US20080308160A1

公开(公告)日：2008-12-18

申请号：US12221604

申请日：2008-08-05

申请人： Steven J. Boege ,  Mark F. Oldham ,  Marc Haberstroh ,  Stephen J. Gunstream ,  Adrian Fawcett ,  Douglas W. Grunewald

发明人： Steven J. Boege ,  Mark F. Oldham ,  Marc Haberstroh ,  Stephen J. Gunstream ,  Adrian Fawcett ,  Douglas W. Grunewald

IPC分类号： F16L53/00

CPC分类号： H05B6/108 ,  B01F13/0818 ,  B01L3/50851 ,  B01L2300/0803 ,  B01L2300/0809 ,  B01L2300/1816 ,  H05B6/109 ,  H05B2214/04 ,  Y10T137/6606

摘要： A device is provided that comprises one or more fluid retainment regions each having at least one wall, and one or more loops in heat-transfer communication with the at least one wall. Each of the loops can comprise an electrical conductor that surrounds the same or a different fluid retainment region. A device is provided that comprises one or more fluid retainment regions each having particulates disposed therein. A system is provided that includes a platen adapted to hold a device including fluid retainment regions and one or more electrical conductors in heat-transfer communication with the fluid retainment regions. Methods of heating a sample are also provided.

摘要翻译： 提供了一种装置，其包括一个或多个流体保持区域，每个流体保持区域各自具有至少一个壁，以及与所述至少一个壁热传递连通的一个或多个环路。 每个环可以包括围绕相同或不同流体保持区域的电导体。 提供了一种装置，其包括一个或多个流体保持区域，每个流体保持区域各自具有设置在其中的微粒。 提供了一种系统，其包括适于保持包括流体保持区域的装置和与流体保持区域传热连通的一个或多个电导体的压板。 还提供了加热样品的方法。

公开(公告)号：US20070295705A1

公开(公告)日：2007-12-27

申请号：US11597399

申请日：2005-05-24

申请人： Andreas Geisbauer

发明人： Andreas Geisbauer

IPC分类号： H05B3/00 ,  G05B15/00 ,  H05B6/02 ,  H05B1/00

CPC分类号： B01L7/5255 ,  B01L3/5088 ,  B01L2300/1805 ,  B01L2300/1816 ,  B01L2300/1827

摘要： Tempering methods are provided for performing a defined, particularly cyclical thermal treatment of small amounts of liquid on substrates. One or several amounts of liquid are applied to a substrate. The substrate is brought in thermal contact with heating apparatus that is started during heating phases of the thermal treatment. A thermally conductive element having thermal capacity greater than or equal to the sum of thermal capacities of the amounts of liquid, substrate and at least part of the heating apparatus in thermal contact with the substrate, is brought in thermal contact with the substrate or the heating apparatus during cooling phases of the thermal treatment. Thermal contact between the thermally conductive element and substrate is interrupted and the heating apparatus started during heating phases of the thermal treatment. A tempering method in which substrates having integrated heating are used as well a tempering device suitable for carrying out these methods, are also provided.

摘要翻译： 提供回火方法用于对基底上的少量液体进行限定的，特别是循环的热处理。 将一种或几种量的液体施加到基底上。 使基板与在热处理的加热阶段期间开始的加热装置进行热接触。 具有大于或等于液体，基板和与基板热接触的加热装置的至少一部分的热容量之和的热容量的导热元件与基板或加热 装置在热处理的冷却阶段。 导热元件和基板之间的热接触被中断，加热装置在热处理的加热阶段开始。 还提供了一种回收方法，其中使用具有集成加热的基板以及适于执行这些方法的回火装置。