公开(公告)号：US20070007148A1

公开(公告)日：2007-01-11

申请号：US11452370

申请日：2006-06-14

申请人： Jun Okada ,  Koji Hashimoto ,  Masayoshi Takahashi

发明人： Jun Okada ,  Koji Hashimoto ,  Masayoshi Takahashi

IPC分类号： G01N27/26

CPC分类号： C12Q1/6825 ,  C12Q2563/173 ,  C12Q2549/125

摘要： A conductive detecting electrode (2), first blocking molecules (21) formed so as to cover a surface of the detecting electrode (2), the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode (2), a target-complementary probe (23) immobilized to the detecting electrode (2) via a spacer member (22) comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection, a conductive comparison electrode (3), and second blocking molecules (31) formed so as to cover a surface of the comparison electrode (3), the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode (3), are provided.

摘要翻译： 导电检测电极（2），形成为覆盖检测电极（2）的表面的第一阻挡分子（21），第一阻挡分子减少插入剂吸附到检测电极（2）的表面， 通过包含直链有机分子的间隔构件（22）固定在检测电极（2）上的目标互补探针（23），靶互补探针包括与作为检测对象的目标碱基序列互补的碱基序列 ，导电比较电极（3）和形成为覆盖比较电极（3）的表面的第二阻挡分子（31），第二阻断分子减少插入剂吸附到比较电极（3）的表面 ）。

公开(公告)号：US20060275817A1

公开(公告)日：2006-12-07

申请号：US11460550

申请日：2006-08-11

申请人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

发明人： J. Parce ,  Theo Nikiforov ,  Tammy Mehta ,  Anne Kopf-Sill ,  Andrea Chow ,  Michael Knapp

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

摘要翻译： 核苷酸和核苷酸类似物通过合成方法的并入/测序用于各种测序。 包含3'-封闭基团的核苷酸类似物用于提供可逆的链终止，用于通过合成进行测序。 典型的封闭基团包括磷酸基和氨基甲酸酯基。 使用荧光核苷酸进行测序，通过掺入荧光标记的核苷酸进行检测，任选地随后进行光漂白，并使用嵌入染料检测通过插入检测合成测序中的未标记核苷酸的添加。 微流控器件，包括粒子阵列，用于测序方法。

公开(公告)号：US07112407B2

公开(公告)日：2006-09-26

申请号：US10687588

申请日：2003-10-20

申请人： Takahiko Ishiguro ,  Juichi Saitoh ,  Tetsuya Ishizuka

发明人： Takahiko Ishiguro ,  Juichi Saitoh ,  Tetsuya Ishizuka

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6865 ,  C12Q2563/173 ,  C12Q2527/125 ,  C12Q2521/107 ,  C12Q2521/119 ,  C12Q2563/107

摘要： A simple and accurate method for assay of a single-stranded RNA containing a specific nucleic acids sequence in a sample at almost constant temperature by using at least the following reagents (A) to (I), which comprises a step of adding the reagents (A) to (I) one by one (in any order), in combinations of at least two or all at once and a step of measuring a fluorescent signal in the presence of the reagent (I) at least once after addition of at least the reagents (A) to (H); (A) a first single-stranded oligonucleic acid complementary to a sequence neighboring the 5′ end of the specific nucleic acids sequence in the single-stranded RNA, (B) a second single-stranded oligo DNA complementary to a 3′-end sequence within the specific nucleic acids sequence, (C) an RNA-dependent DNA polymerase, (D) deoxyribonucleoside triphosphates, (E) a third single-stranded oligo DNA having (1) a promoter sequence for a DNA-dependent RNA polymerase, (2) an enhancer sequence for the promoter and (3) a 5′-end sequence within the specific nucleic acids sequence, in this order from the 5′ end, (F) a DNA-dependent DNA polymerase, (G) a DNA-dependent RNA polymerase,

摘要翻译： 通过使用至少以下试剂（A）至（I），在几乎恒定温度下测定样品中含有特定核酸序列的单链RNA的简单且准确的方法，其包括将试剂（ A）至（I）（以任何顺序），至少两个或全部同时组合，以及在至少加入至少一个试剂（I）之后至少测量一次荧光信号的步骤 试剂（A）〜（H）; （A）与单链RNA中特异性核酸序列的5'末端相邻的序列互补的第一单链寡核酸，（B）与3'-末端序列互补的第二单链寡核苷酸 在特定核酸序列中，（C）RNA依赖性DNA聚合酶，（D）脱氧核糖核苷三磷酸，（E）具有（1）DNA依赖性RNA聚合酶的启动子序列的第三单链寡核苷酸（2） ）启动子的增强子序列和（3）特异性核酸序列内的5'端序列，从5'端起依次为（F）DNA依赖性DNA聚合酶，（G）DNA依赖性 RNA聚合酶，

公开(公告)号：US20060211028A1

公开(公告)日：2006-09-21

申请号：US11377253

申请日：2006-03-16

申请人： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

发明人： Fei Mao ,  Wai-Yee Leung ,  Xing Xin

IPC分类号： C12Q1/68 ,  C07D401/02

CPC分类号： C12Q1/6876 ,  C07D209/08 ,  C07D213/38 ,  C07D221/10 ,  C07D417/06 ,  C07D417/14 ,  C07D491/147 ,  C07H21/00 ,  C09B15/00 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2563/173

摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.

摘要翻译： 提供二聚和三聚体核酸染料以及相关系统和方法。 这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。 在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。 本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

公开(公告)号：US07105300B2

公开(公告)日：2006-09-12

申请号：US10413049

申请日：2003-04-14

申请人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

发明人： J. Wallace Parce ,  Theo T. Nikiforov ,  Tammy Burd Mehta ,  Anne R. Kopf-Sill ,  Andrea W. Chow ,  Michael R. Knapp

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H19/04

CPC分类号： C12Q1/6874 ,  B01F13/0059 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00468 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6869 ,  C40B60/14 ,  G01N27/44756 ,  G01N35/0098 ,  G01N2035/00574 ,  Y10T436/143333 ,  C12Q2565/629 ,  C12Q2535/101 ,  C12Q2563/173 ,  C12Q2525/186 ,  C12Q2527/125

摘要： Nucleotides and nucleotide analogs are used in various sequencing by incorporation/sequencing by synthesis methods. Nucleotide analogs comprising 3′-blocking groups are used to provide reversible chain-termination for sequencing by synthesis. Typical blocking groups include phosphate groups and carbamate groups. Fluorescent nucleotides are used to perform sequencing by synthesis with detection by incorporation of the fluorescently labeled nucleotide, optionally followed by photobleaching and intercalating dyes are used to detect addition of a non-labeled nucleotide in sequencing by synthesis with detection by intercalation. Microfluidic devices, including particle arrays, are used in the sequencing methods.

公开(公告)号：US20060177844A1

公开(公告)日：2006-08-10

申请号：US11262523

申请日：2005-10-27

申请人： Jesus Ching ,  Ronald Chang ,  Douglas Dority ,  Jian Ping Zhang ,  James Wang ,  Wendy Wong ,  Kendra Paul ,  Reuel Van Atta

发明人： Jesus Ching ,  Ronald Chang ,  Douglas Dority ,  Jian Ping Zhang ,  James Wang ,  Wendy Wong ,  Kendra Paul ,  Reuel Van Atta

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： G01N21/6428 ,  B01L3/502 ,  B01L7/52 ,  B01L2200/0684 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2400/0487 ,  B01L2400/0644 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2563/173 ,  C12Q2549/119 ,  C12Q2531/143 ,  C12Q2537/149

摘要： The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

摘要翻译： 本发明涉及用于进行多级扩增反应的系统，方法和装置，特别是在流体封闭的条件下。 在一个方面，本发明的方法在流体封闭的反应体系中进行，其允许分离第一（或先前）反应混合物的一部分，并将其用作第二（或后续）反应混合物中的样品或样品 ，从而基本上避免了如果两个反应混合物简单地组合在一起，第一反应组分可能在第二反应中可能具有干扰效应。 在这方面，本发明的系统，方法和装置可以与基于嵌套引物的使用允许多个扩增阶段的任何扩增反应一起使用。

公开(公告)号：US07052844B2

公开(公告)日：2006-05-30

申请号：US10269229

申请日：2002-10-11

申请人： Ian J. Atkinson ,  Glen H. Erikson ,  Jasmine I. Daksis ,  Pierre Picard

发明人： Ian J. Atkinson ,  Glen H. Erikson ,  Jasmine I. Daksis ,  Pierre Picard

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6839 ,  C12Q2563/173 ,  C12Q2563/107 ,  C12Q2537/119 ,  C12Q2527/125 ,  C12Q2563/137

摘要： A purification method includes bonding a probe to a target in a sample to form a complex, and separating the sample from the complex to separate in a sequence specific manner the target from the sample. The complex, which is immobilized on a support, is a duplex, triplex or quadruplex formed by Watson-Crick complementary base interaction or by homologous base interaction, provided that when the complex is a duplex and the heteropolymeric probe sequence is antiparallel to the heteropolymeric target sequence, the heteropolymeric probe sequence is bonded to the heteropolymeric target sequence by homologous base interaction, and provided that when the complex is a triplex, the complex is free of recombination proteins. A kit for performing the method includes the support and the probe.

公开(公告)号：US20060014152A1

公开(公告)日：2006-01-19

申请号：US10867994

申请日：2004-06-15

申请人： Martin Mautner

发明人： Martin Mautner

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/6858 ,  C12Q1/6869 ,  C12Q2563/173 ,  C12Q2527/125 ,  C12Q2535/125

摘要： A method for reducing the efficiency of primer extension by polymerase enzymes when the 3′ end of a primer or growing nucleic acid chain does not hybridize perfectly with the target, for increasing the selectivity of single nucleotide mutation or gene analyses, for suppressing false positive results and for enhancing the fidelity of the amplification of nucleic acid fragments by avoiding the incorporation of mispairs, the method comprising the steps of: (a) obtaining a nucleic acid sample; (b) hybridizing said nucleic acid sample to a primer; (c) subjecting said nucleic acid sample hybridized to a extension reaction by extending a primer with a polymerizing enzyme; and (d) detecting the presence of extension products; wherein the reaction extension mixture medium contains an intercalating agent such as ethidium bromide, dihydroethidium, ethidium homodimer-1, ethidium homodimer-2, acridine, propidium iodide, YOYO®-1 or TOTO®-1. When the intercalating agent is ethidium bromide the concentration is about 1 to 7 μg/ml.

摘要翻译： 当引物或生长核酸链的3'末端与靶完全杂交时，为提高单核苷酸突变或基因分析的选择性，抑制假阳性结果，降低聚合酶引物延伸效率的方法 并且为了通过避免引入错配来增强核酸片段的扩增的保真度，该方法包括以下步骤：（a）获得核酸样品; （b）将所述核酸样品与引物杂交; （c）通过用聚合酶延伸引物对经过延伸反应杂交的所述核酸样品进行处理; 和（d）检测延伸产品的存在; 其中所述反应延伸混合物介质含有插层剂，例如溴化乙锭，二氢乙硫醚，同系二聚体同族二聚体-1，同系二聚体-2，吖啶，碘化丙啶，YOYO或TOTO。 当插入剂是溴化乙锭时，浓度为约1至7mug / ml。

公开(公告)号：US20050266404A1

公开(公告)日：2005-12-01

申请号：US10484856

申请日：2002-07-25

申请人： Jong-Hoon Hahn ,  Nkoyoung Park

发明人： Jong-Hoon Hahn ,  Nkoyoung Park

IPC分类号： C12M1/34 ,  C12Q1/68

CPC分类号： C12Q1/6825 ,  C12Q1/6837 ,  C12Q2565/501 ,  C12Q2522/101 ,  C12Q2563/173 ,  C12Q2565/607

摘要： The present invention relates to a detection method of DNA hybridization comprising: (a) preparing a oligo-plate by fixing capture probes onto a working electrode surface, (b) hybridizing the capture probes with target probes, (c) reacting a nucleic acid binding material specific to single-stranded nucleic acid or a double-stranded nucleic acid, (d) thc nucleic acid binding material changes the charge state of the working electrode surface, and (e) measuring an electrochemical quantity depending on the charge state of the working electrode surface with an electrochemical method using an electroactive electrolytic condition.

摘要翻译： 本发明涉及DNA杂交的检测方法，包括：（a）通过将捕获探针固定在工作电极表面上来制备寡聚板，（b）使捕获探针与靶探针杂交，（c）使核酸结合 对单链核酸或双链核酸特异的材料，（d）核酸结合材料改变工作电极表面的电荷状态，（e）根据工作电荷的充电状态测量电化学量 电极表面使用电活性电解条件的电化学方法。

公开(公告)号：US20050255485A1

公开(公告)日：2005-11-17

申请号：US10881368

申请日：2004-06-30

申请人： Kenneth Livak ,  Junko Stevens ,  Katherine Lazaruk ,  Janet Ziegle ,  Lily Wong

发明人： Kenneth Livak ,  Junko Stevens ,  Katherine Lazaruk ,  Janet Ziegle ,  Lily Wong

IPC分类号： C12Q1/68

CPC分类号： G16B20/00 ,  C12Q1/6827 ,  C12Q1/6851 ,  C12Q2600/156 ,  C12Q2563/107 ,  C12Q2545/101 ,  C12Q2565/101 ,  C12Q2563/173 ,  C12Q2565/1015

摘要： Methods of detecting a candidate genetic anomaly such as a candidate duplication in a genome are disclosed. The methods comprise quantifying fluorogenic assays for alleles of a genetic locus from a plurality of individual genomes, identifying ranges of fluorescent intensities indicative of individual genomes homozygous for a first allele, homozygous for a second allele, or heterozygous for both alleles, and identifying individual genomes in which the fluorescence intensities are outside the range of intensities indicative of homozygosity or heterozygosity for the genetic locus.

摘要翻译： 公开了检测候选遗传异常（例如基因组中候选重复）的方法。 所述方法包括定量来自多个单个基因组的遗传基因座的等位基因的荧光测定，鉴定指示第一等位基因纯合的单个基因组的荧光强度的范围，对于第二等位基因纯合，或对于两个等位基因是杂合的，并鉴定个体基因组 其中荧光强度在表示遗传基因座的纯合性或杂合性的强度范围之外。