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    PURIFICATION SYSTEMS AND METHODS
    62.
    发明申请
    PURIFICATION SYSTEMS AND METHODS 有权

    公开(公告)号：US20170174846A1

    公开(公告)日：2017-06-22

    申请号：US15392834

    申请日：2016-12-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wolfgang HINZ ,  Ryan JONES ,  David LIGHT

    IPC: C08J3/12 ,  B07B1/00 ,  C12N15/10

    CPC classification number: C08J3/12 ,  B07B1/00 ,  C08J2300/14 ,  C12N15/1017

    Abstract: A method of preparing hydrogel particles includes applying a solution including a plurality of hydrogel particles to a stir cell. A retentate side of a filter defines a lower surface of the stir cell. The filter has the retentate side and a permeate side. The method further includes, while stirring the solution within the stir cell, dispensing a buffer solution at a first flow rate to the stir cell and drawing a permeate from the permeate side of the filter using a pump at a second flow rate, the permeate including a subset of the plurality of hydrogel particles.

    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME
    64.
    发明申请
    MODIFIED PROTEINS AND METHODS OF MAKING AND USING SAME 有权
    改良蛋白质及其制备方法和使用方法

    公开(公告)号：US20150260680A1

    公开(公告)日：2015-09-17

    申请号：US14724354

    申请日：2015-05-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

    IPC: G01N27/414

    CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

    Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

    Abstract translation: 提供了包含修饰的蛋白质，特别是具有改变的缓冲性质的经修饰的核酸结合蛋白质的方法，组合物，系统，装置和试剂盒。 例如，在一些实施方案中，描述了形成包含一个或多个氨基酸修饰以获得所需pKa值的修饰蛋白质的方法。 此外，本发明提供了在离子产生反应中使用这些修饰蛋白质的方法，例如基于离子的核酸测序反应。

    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE
    65.
    发明申请
    CHEMICAL SENSOR WITH CONDUCTIVE CUP-SHAPED SENSOR SURFACE 审中-公开
    具有导电杯形传感器表面的化学传感器

    公开(公告)号：US20140220697A1

    公开(公告)日：2014-08-07

    申请号：US14260818

    申请日：2014-04-24

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Shifeng LI ,  James BUSTILLO ,  Wolfgang HINZ

    IPC: G01N27/414

    CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  G01N27/414 ,  Y10T436/143333

    Abstract: A system includes a sensor including a sensor pad and a well wall structure defining a well operatively coupled to the sensor pad. The well is further defined by a lower surface disposed over the sensor pad. The well wall structure defines an upper surface and defines a wall surface extending between the upper surface and the lower surface. The system further includes a conductive layer disposed over the lower surface and the wall surface.

    Abstract translation: 一种系统包括传感器，其包括传感器垫和井壁结构，其限定了良好地操作地耦合到传感器垫的结构。 该井由设置在传感器垫上的下表面进一步限定。 井壁结构限定了上表面并且限定了在上表面和下表面之间延伸的壁表面。 该系统还包括设置在下表面和壁表面上的导电层。

    NUCLEIC ACID AMPLIFICATION
    66.
    发明申请
    NUCLEIC ACID AMPLIFICATION 有权
    核酸放大

    公开(公告)号：US20140080717A1

    公开(公告)日：2014-03-20

    申请号：US14023361

    申请日：2013-09-10

    Applicant: Life Technologies Corporation

    Inventor： Chieh-Yuan LI ,  David RUFF ,  Shiaw-Min CHEN ,  Jennifer O'NEIL ,  Rachel KASINSKAS ,  Jonathan ROTHBERG ,  Wolfgang HINZ

    IPC: C12Q1/68

    CPC classification number: C12N9/1252 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Y207/07007 ,  C12Q2531/119 ,  C12Q2565/537

    Abstract: In some embodiments, the present teachings provide methods for nucleic acid amplification, comprising forming a reaction mixture, and subjecting the reaction mixture to conditions suitable for nucleic acid amplification. In some embodiments, methods for nucleic acid amplification include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, methods for nucleic acid amplification include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the methods for nucleic acid amplification employ an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel. In some embodiments, methods for nucleic acid amplification can be conducted in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise a amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer. The polymer can include a sieving agent and/or a diffusion-reducing agent.

    Abstract translation: 在一些实施方案中，本教导提供核酸扩增方法，包括形成反应混合物，并使反应混合物经受适合于核酸扩增的条件。 在一些实施方案中，用于核酸扩增的方法包括将待扩增的核酸进行部分变性的条件。 在一些实施方案中，用于核酸扩增的方法包括扩增而不完全变性扩增的核酸。 在一些实施方案中，用于核酸扩增的方法使用催化同源重组的酶和聚合酶。 在一些实施方案中，用于核酸扩增的方法可以在单个反应容器中进行。 在一些实施方案中，用于核酸扩增的方法可以在反应混合物的单个连续液相中进行，而不需要分隔反应混合物或固定反应组分。 在一些实施方案中，用于核酸扩增的方法包括在等温扩增条件下任选地在聚合物存在下在表面上扩增至少一种多核苷酸。 聚合物可以包括筛分剂和/或扩散还原剂。

    CHEMICALLY SENSITIVE SENSORS WITH SAMPLE AND HOLD CAPACITORS
    67.
    发明申请
    CHEMICALLY SENSITIVE SENSORS WITH SAMPLE AND HOLD CAPACITORS 有权
    具有样品和保持电容器的化学感应传感器

    公开(公告)号：US20130264611A1

    公开(公告)日：2013-10-10

    申请号：US13922149

    申请日：2013-06-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

    IPC: H01L29/78

    CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

    Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。

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