公开(公告)号：US07323143B2

公开(公告)日：2008-01-29

申请号：US10303642

申请日：2002-11-25

申请人： Janelle R. Anderson ,  Daniel T. Chiu ,  Rebecca J. Jackman ,  Oksana Cherniavskaya ,  Justin Cooper McDonald ,  George M. Whitesides

发明人： Janelle R. Anderson ,  Daniel T. Chiu ,  Rebecca J. Jackman ,  Oksana Cherniavskaya ,  Justin Cooper McDonald ,  George M. Whitesides

IPC分类号： B01L3/02 ,  B01L11/00 ,  B32B5/05 ,  B32B27/04 ,  B32B27/12

CPC分类号： B29C33/3857 ,  B01D61/18 ,  B01D67/003 ,  B01D67/0034 ,  B01D69/02 ,  B01D71/70 ,  B01D2323/24 ,  B01D2323/34 ,  B01D2325/028 ,  B01D2325/08 ,  B01L3/502707 ,  B01L2300/0874 ,  B01L2300/0887 ,  B29C33/3892 ,  B29C33/52 ,  B29C35/0888 ,  B29C39/021 ,  B29C39/34 ,  B29C2035/0827 ,  B33Y80/00 ,  B81B2201/058 ,  B81C1/00119 ,  B81C2201/019 ,  Y10T436/11 ,  Y10T436/2575

摘要： The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography. The microfluidic networks of the invention are, in some cases, comprised of a single replica molded layer, and, in other cases, are comprised of two, three, or more replica molded layers that have been assembled to form the overall microfluidic network structure. The present invention also describes various novel applications for using the microfluidic network structures provided by the invention.

公开(公告)号：US07288394B2

公开(公告)日：2007-10-30

申请号：US10668679

申请日：2003-09-23

申请人： Emanuele Ostuni ,  Christopher S. Chen ,  Donald E. Ingber ,  George M. Whitesides

发明人： Emanuele Ostuni ,  Christopher S. Chen ,  Donald E. Ingber ,  George M. Whitesides

IPC分类号： C12N11/02 ,  C12N11/08 ,  C07K17/02 ,  C07K17/08

CPC分类号： B01L3/5085 ,  B01J2219/00317 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00743 ,  B01L3/5088 ,  C12N11/08 ,  Y10S530/812 ,  Y10S530/815

摘要： A method of patterning materials, such as proteins, on a contoured surface by depositing them onto protrusions on a surface, and a cell containment device that may be constructed by this method, are provided. The method may involve selectively depositing a material on a substrate including a contoured surface including protrusions and recesses. By applying a first fluid to the contoured surface and allowing the first fluid to distribute across only a portion of the contoured surface, a material may be deposited on the protrusions and not the recesses, or on the recesses and not the protrusions. Such a method may be used to selectively pattern cells or other materials on substrates.

摘要翻译： 提供了通过将材料沉积在表面上的突起上而在轮廓表面上图案化材料（例如蛋白质）的方法，以及可以通过该方法构造的电池容纳装置。 该方法可以包括选择性地将材料沉积在包括突起和凹陷的轮廓表面的基底上。 通过将第一流体施加到轮廓表面并且允许第一流体仅跨越轮廓表面的一部分分布，材料可以沉积在突起上而不是凹部上，或者凹部上而不是突出部。 这种方法可以用于选择性地在基底上图案化细胞或其他材料。

公开(公告)号：US07198747B2

公开(公告)日：2007-04-03

申请号：US09940072

申请日：2001-08-27

申请人： Hong Yang ,  Pascal Deschatelets ,  George M. Whitesides

发明人： Hong Yang ,  Pascal Deschatelets ,  George M. Whitesides

IPC分类号： B23B1/00 ,  C04B35/64

CPC分类号： B81C99/008 ,  B28B7/342 ,  B28B13/021 ,  B81C99/0085 ,  B81C2201/034 ,  B82Y10/00 ,  B82Y30/00 ,  C04B35/571 ,  C04B35/589 ,  C04B35/622 ,  Y10T428/26

摘要： The invention provides ceramic molded solid articles and methods for making these articles on the micron scale. Articles are molded from ceramic precursors, optionally using molds including at least one portion that is elastomeric.

摘要翻译： 本发明提供陶瓷模制固体制品和用于制造这些制品的微米级别的方法。 制品由陶瓷前体模制，任选地使用包括弹性体的至少一部分的模具。

公开(公告)号：US07067306B2

公开(公告)日：2006-06-27

申请号：US10032280

申请日：2001-12-21

申请人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

发明人： Rahul Singhvi ,  Amit Kumar ,  George M. Whitesides ,  Donald E. Ingber ,  Gabriel P. Lopez ,  Daniel I. C. Wang ,  Gregory N. Stephanopoulos

IPC分类号： C12Q1/02 ,  C12Q1/04 ,  C12N11/02 ,  C12N11/08

CPC分类号： C12N1/00 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/0072 ,  B81B2201/0214 ,  B81C1/00206 ,  B81C2201/034 ,  B82Y5/00 ,  B82Y30/00 ,  C12N5/0068 ,  C12N5/067 ,  C12N2535/10 ,  C12Q1/02 ,  C40B60/14

摘要： The invention provides a device for adhering cells in a specific and predetermined position, and associated methods. The device includes a plate defining a surface and a plurality of cytophilic islands that adhere cells, isolated by cytophobic regions to which cells do not adhere, contiguous with the cytophilic islands. The islands or the regions or both may be formed of a self-assembled monolayer (SAM).

摘要翻译： 本发明提供一种用于将细胞粘附在特定和预定位置的装置以及相关方法。 该装置包括限定表面的板和多个细胞亲和岛，其附着细胞，由细胞不粘附的细胞渗透区隔离，与细胞色素岛邻接。 岛或区域或两者可以由自组装单层（SAM）形成。

公开(公告)号：US06747285B2

公开(公告)日：2004-06-08

申请号：US10003033

申请日：2001-11-01

申请人： Olivier J. A. Schueller ,  David C. Duffy ,  John A. Rogers ,  Scott T. Brittain ,  George M. Whitesides

发明人： Olivier J. A. Schueller ,  David C. Duffy ,  John A. Rogers ,  Scott T. Brittain ,  George M. Whitesides

IPC分类号： G01N1506

CPC分类号： B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/0689 ,  B01L2300/0654 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2400/0418 ,  G01N21/45 ,  G01N21/4788 ,  Y10T428/24562 ,  Y10T428/2457 ,  Y10T428/24744 ,  Y10T428/31663

摘要： A system is provided for positioning separate portions of a sample in elongate, parallel channels of a sample chamber and for irradiating a sample in the chamber to create a diffraction pattern where the sample and chamber differ in refractive index. The system also can measure absorption of electromagnetic radiation by a sample in the chamber, and can measure the absorption simultaneously with measurement of diffraction by the sample.

摘要翻译： 提供了一种用于将样品的分离部分定位在样品室的细长平行通道中并用于在室中照射样品以产生样品和室的折射率不同的衍射图案的系统。 该系统还可以测量室内样品对电磁辐射的吸收，并可以通过样品的衍射测量同时测量吸收。

公开(公告)号：US06716378B2

公开(公告)日：2004-04-06

申请号：US10145658

申请日：2002-05-14

申请人： Peidong Yang ,  Tao Deng ,  George M. Whitesides ,  Galen Stucky ,  Dongyaun Zhao ,  Bradley Chmelka ,  David Pine ,  Pingyun Feng

发明人： Peidong Yang ,  Tao Deng ,  George M. Whitesides ,  Galen Stucky ,  Dongyaun Zhao ,  Bradley Chmelka ,  David Pine ,  Pingyun Feng

IPC分类号： B29C6500

CPC分类号： C04B35/622 ,  B82Y30/00 ,  C04B35/624 ,  C04B38/0051 ,  C04B2111/00405 ,  C04B35/01 ,  C04B38/0045

摘要： A low-cost, efficient method of preparing hierarchically ordered structures by filling a mold with a self-assembling mixture of hydrolyzed inorganic species and amphiphilic block copolymers and applying pressure to the mixture. Polymerization of the inorganic species within the mixture results in a mesoscopically structured material having molded features. A mesoporous material can be produced by subsequent thermal removal of the copolymers.

摘要翻译： 通过用水解无机物质和两亲性嵌段共聚物的自组装混合物填充模具来制备分层结构的低成本，有效的方法，并向混合物施加压力。 混合物内的无机物质的聚合导致具有成型特征的中视结构材料。 可以通过随后热去除共聚物来制备介孔材料。

公开(公告)号：US06660192B1

公开(公告)日：2003-12-09

申请号：US09634201

申请日：2000-08-09

申请人： Enoch Kim ,  Younan Xia ,  Milan Mrksich ,  Rebecca J. Jackman ,  Xiao-Mei Zhao ,  Stephen P. Smith ,  Mara G. Prentiss ,  George M. Whitesides ,  Christian Marzolin

发明人： Enoch Kim ,  Younan Xia ,  Milan Mrksich ,  Rebecca J. Jackman ,  Xiao-Mei Zhao ,  Stephen P. Smith ,  Mara G. Prentiss ,  George M. Whitesides ,  Christian Marzolin

IPC分类号： B29D1100

CPC分类号： B81C1/00206 ,  A61K47/6957 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00416 ,  B01J2219/0043 ,  B01J2219/00466 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00713 ,  B29C31/04 ,  B29C33/42 ,  B29C37/0053 ,  B29C39/24 ,  B29C43/021 ,  B29C67/246 ,  B29D11/00346 ,  B29D11/00663 ,  B29K2105/0002 ,  B29L2011/0016 ,  B81B2201/0214 ,  B81C1/00031 ,  B81C99/0085 ,  B81C2201/034 ,  B82Y30/00 ,  C40B60/14 ,  G01N21/7703 ,  G01N2021/7776 ,  G02B6/138 ,  H05K3/0079 ,  H05K3/061

摘要： Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or non-planar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface. Waveguide arrays, combinatorial chemical or biochemical libraris, etc. can be made. Differences in refractive index of waveguide and cladding can be created by subjecting the waveguide and cladding, made of indentical prepolymeric material, to different polymerization or cross-linking conditions. Interferometers are defined by coupling arrays of waveguides, where coupling can be controlled by altering the difference in refractive index between cladding and waveguide at any desired location of the array. Alteration and refractive index can be created photochemically, chemically, or the like. Sensors also are disclosed, including biochemical sensors.

摘要翻译： 化学或生物化学活性剂或其它物质通过提供具有轮廓表面的微胶体并在基底表面上形成结构的化学或生物化学活性剂或流体前体而在基底表面上图案化。 化学或生物化学活性剂或流体前体也可以从敷料器中的凹陷转移到基底表面。 衬底表面可以是平面的或非平面的。 聚合物结构，无机陶瓷和盐等的流体前体可用于在表面形成图案化聚合物制品，无机盐和陶瓷，反应离子蚀刻掩模等。 制品可以形成为包括横向尺寸小于约1毫米或更小的部分的图案。 涂抹器的压痕图案可以用于将单独的，不同的化学或生物化学活性剂或流体前体转移到基底表面的分离的，分离的区域。 可以制作波导阵列，组合化学或生化图谱等。 波导和包层的折射率差异可以通过将由凹凸预聚物材料制成的波导和包层进行不同的聚合或交联条件来产生。 干涉仪通过耦合波导阵列来定义，其中可以通过改变阵列的任何所需位置处的包层和波导之间的折射率差来控制耦合。 改变和折射率可以通过光化学，化学等方式产生。 还公开了传感器，包括生化传感器。

公开(公告)号：US06416762B1

公开(公告)日：2002-07-09

申请号：US09210282

申请日：1998-12-11

申请人： Diane M. Joseph-McCarthy ,  Lyle D. Isaacs ,  George M. Whitesides ,  Martin Karplus ,  James M. Hogle ,  James Li-wen Cheh

发明人： Diane M. Joseph-McCarthy ,  Lyle D. Isaacs ,  George M. Whitesides ,  Martin Karplus ,  James M. Hogle ,  James Li-wen Cheh

IPC分类号： A61K39125

CPC分类号： A61K47/6901 ,  Y10T436/14 ,  Y10T436/141111 ,  Y10T436/147777

摘要： The present invention provides structure-based combinatorial libraries of compounds containing the functional group minima of picornaviruses including poliovirus and rhinovirus. The libraries can be used to screen for therapeutical antiviral compounds, e.g., anti-picornaviral capsid-binding compounds.

摘要翻译： 本发明提供了包含小核糖核酸病毒（包括脊髓灰质炎病毒和鼻病毒）的功能组最小值的化合物的基于结构的组合文库。 文库可以用于筛选治疗性抗病毒化合物，例如抗小RNA病毒衣壳结合化合物。

公开(公告)号：US06355198B1

公开(公告)日：2002-03-12

申请号：US09004583

申请日：1998-01-08

申请人： Enoch Kim ,  Younan Xia ,  Milan Mrksich ,  Rebecca J. Jackman ,  Xiao-Mei Zhao ,  Stephen P. Smith ,  Mara G. Prentiss ,  George M. Whitesides ,  Christian Marzolin

发明人： Enoch Kim ,  Younan Xia ,  Milan Mrksich ,  Rebecca J. Jackman ,  Xiao-Mei Zhao ,  Stephen P. Smith ,  Mara G. Prentiss ,  George M. Whitesides ,  Christian Marzolin

IPC分类号： B29C3910

CPC分类号： B81C1/00206 ,  A61K47/6957 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00416 ,  B01J2219/0043 ,  B01J2219/00466 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00713 ,  B29C31/04 ,  B29C33/42 ,  B29C37/0053 ,  B29C39/24 ,  B29C43/021 ,  B29C67/246 ,  B29D11/00346 ,  B29D11/00663 ,  B29K2105/0002 ,  B29L2011/0016 ,  B81B2201/0214 ,  B81C1/00031 ,  B81C99/0085 ,  B81C2201/034 ,  B82Y30/00 ,  C40B60/14 ,  G01N21/7703 ,  G01N2021/7776 ,  G02B6/138 ,  H05K3/0079 ,  H05K3/061

摘要： Chemically or biochemically active agents or other species are patterned on a substrate surface by providing a micromold having a contoured surface and forming, on a substrate surface, a chemically or biochemically active agent or fluid precursor of a structure. A chemically or biochemically active agent or fluid precursor also can be transferred from indentations in an applicator to a substrate surface. The substrate surface can be planar or nonplanar. Fluid precursors of polymeric structures, inorganic ceramics and salts, and the like can be used to form patterned polymeric articles, inorganic salts and ceramics, reactive ion etch masks, etc. at the surface. The articles can be formed in a pattern including a portion having a lateral dimension of less than about 1 millimeter or smaller. The indentation pattern of the applicator can be used to transfer separate, distinct chemically or biochemically active agents or fluid precursors to separate, isolated regions of a substrate surface. Waveguide arrays, combinatorial chemical or biochemical libraris, etc. can be made. Differences in refractive index of waveguide and cladding can be created by subjecting the waveguide and cladding, made of indentical prepolymeric material, to different polymerization or cross-linking conditions. Interferometers are defined by coupling arrays of waveguides, where coupling can be controlled by altering the difference in refractive index between cladding and waveguide at any desired location of the array. Alteration and refractive index can be created photochemically, chemically, or the like. Sensors also are disclosed, including biochemical sensors.

摘要翻译： 化学或生物化学活性剂或其它物质通过提供具有轮廓表面的微胶体并在基底表面上形成结构的化学或生物化学活性剂或流体前体而在基底表面上图案化。 化学或生物化学活性剂或流体前体也可以从敷料器中的凹陷转移到基底表面。 衬底表面可以是平面或非平面的。 聚合物结构，无机陶瓷和盐等的流体前体可用于在表面形成图案化聚合物制品，无机盐和陶瓷，反应离子蚀刻掩模等。 制品可以形成为包括横向尺寸小于约1毫米或更小的部分的图案。 涂抹器的压痕图案可以用于将单独的，不同的化学或生物化学活性剂或流体前体转移到基底表面的分离的，分离的区域。 可以制作波导阵列，组合化学或生化图谱等。 波导和包层的折射率差异可以通过将由凹凸预聚物材料制成的波导和包层进行不同的聚合或交联条件来产生。 干涉仪通过耦合波导阵列来定义，其中可以通过改变阵列的任何所需位置处的包层和波导之间的折射率差来控制耦合。 改变和折射率可以通过光化学，化学等方式产生。 还公开了传感器，包括生化传感器。

公开(公告)号：US06180239B2

公开(公告)日：2001-01-30

申请号：US08676951

申请日：1996-07-08

申请人： George M. Whitesides ,  Younan Xia ,  James L. Wilbur ,  Rebecca J. Jackman ,  Enoch Kim ,  Mara G. Prentiss ,  Milan Mrksich ,  Amit Kumar ,  Christopher B. Gorman ,  Hans Biebuyck ,  Karl K. Berggren

发明人： George M. Whitesides ,  Younan Xia ,  James L. Wilbur ,  Rebecca J. Jackman ,  Enoch Kim ,  Mara G. Prentiss ,  Milan Mrksich ,  Amit Kumar ,  Christopher B. Gorman ,  Hans Biebuyck ,  Karl K. Berggren

IPC分类号： B32B904

CPC分类号： B05D1/283 ,  B01J2219/00382 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01L3/5085 ,  B01L3/5088 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0851 ,  B82Y10/00 ,  B82Y30/00 ,  B82Y40/00 ,  C40B60/14 ,  G02B5/1857 ,  G02B6/124 ,  G02B6/136 ,  G02B2006/12102 ,  G02B2006/12104 ,  G02B2006/12107 ,  G03F7/0002 ,  G03F7/165 ,  H01L21/4867 ,  H01L21/768 ,  H01L24/95 ,  H01L2224/83192 ,  Y10T428/24802 ,  Y10T428/31504

摘要： Improved method of forming a patterned self-assembled monolayer on a surface and derivative articles are provided. According to one method, an elastomeric stamp is deformed during and/or prior to using the stamp to print a self-assembled molecular monolayer on a surface. According to another method, during monolayer printing the surface is contacted with a liquid that is immiscible with the molecular monolayer-forming species to effect controlled reactive spreading of the monolayer on the surface. Methods of printing self-assembled molecular monolayers on nonplanar surfaces and derivative articles are provided, as are methods of etching surfaces patterned with self-assembled monolayers, including methods of etching silicon. Optical elements including flexible diffraction gratings, mirrors, and lenses are provided, as are methods for forming optical devices and other articles using lithographic molding. A method for controlling the shape of a liquid on the surface of an article is provided, involving applying the liquid to a self-assembled monolayer on the surface, and controlling the electrical potential of the surface.

摘要翻译： 提供了在表面和衍生物品上形成图案化自组装单层的改进方法。 根据一种方法，弹性印模在使用印模期间和/或之前变形，以在表面上印刷自组装分子单层。 根据另一种方法，在单层印刷过程中，表面与与分子单层形成物质不混溶的液体接触，以实现单层在表面上的受控的反应性扩散。 提供了在非平面表面和衍生物上印刷自组装分子单层的方法，以及用自组装单层蚀刻表面的方法，包括蚀刻硅的方法。 提供包括柔性衍射光栅，反射镜和透镜的光学元件，以及用于使用光刻成型形成光学器件和其它制品的方法。 提供了一种用于控制制品表面上的液体形状的方法，包括将液体施加到表面上的自组装单层，并控制表面的电位。