公开(公告)号：US20140350232A1

公开(公告)日：2014-11-27

申请号：US14291540

申请日：2014-05-30

申请人： Bioneer Corporation

发明人： Bo Ram Han ,  Han Oh Park ,  Mi Sik Shin ,  Sam Young Lee

IPC分类号： C12N15/113

CPC分类号： A61K47/48215 ,  A61K9/1075 ,  A61K47/34 ,  A61K47/60 ,  B82Y5/00 ,  C07F7/0836 ,  C07H21/02 ,  C08G65/3356 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/351 ,  C12N2310/3515 ,  C12N2320/30 ,  C12N2320/51 ,  Y10S977/773 ,  Y10S977/906

摘要： Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

摘要翻译： 提供了siRNA-聚合物缀合物及其制备方法，更具体地，涉及通过共价键合siRNA和高分子化合物形成的用于提高siRNA体内稳定性的杂合偶联物，以及该混合物的制备方法 缀合物。 本发明的缀合物可以提高siRNA的体内稳定性，从而实现治疗性siRNA有效递送到细胞中，并且即使用相对低浓度的小剂量也显示出siRNA的活性。 因此，缀合物不仅可以有效地用作癌症和其它感染性疾病的siRNA治疗工具，还可以用作新型siRNA递送系统。

公开(公告)号：US20130157275A1

公开(公告)日：2013-06-20

申请号：US13782796

申请日：2013-03-01

申请人： BIONEER CORPORATION

发明人： Han Oh PARK ,  Jae-Don LEE ,  Gu-Young SONG ,  Jong Wak OH ,  Hyun-Bae KIM ,  Sang-Jin BYUN ,  Jin-Wong JANG ,  Won-Seok JANG ,  Jong-Deok CHOI

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： G01N33/58 ,  C12Q1/68 ,  C12Q2531/113 ,  C12Q2561/113 ,  C12Q2563/185

摘要： Provided are an oligonucleotide marker and a method of identifying a material using the same. The oligonucleotide marker makes it possible to analyze a trace amount of the material with high precision within a short time, has improved solubility in an oily solvent, and can improve a detection method such that the oligonucleotide marker can be detected within 2 hours. The oligonucleotide marker can label various products, including oil products and petroleum products, works of art and collections, and can also be used to conduct criminal investigations.

摘要翻译： 提供寡核苷酸标记物和识别使用其的材料的方法。 寡核苷酸标记使得可以在短时间内高精度地分析痕量的材料，在油性溶剂中的溶解性提高，并且可以改进检测方法，使得可以在2小时内检测寡核苷酸标记。 寡核苷酸标记可以标注各种产品，包括石油产品和石油产品，艺术品和收藏品，也可用于进行刑事调查。

公开(公告)号：US20020082416A1

公开(公告)日：2002-06-27

申请号：US10029281

申请日：2001-12-28

申请人： BIONEER CORPORATION

发明人： Han Oh Park ,  In Woo Lee

IPC分类号： C07H021/04

CPC分类号： C12N15/1003

摘要： A method for obtaining DNA from fish spermatogonia comprises (i) disrupting fish spermatogonium to obtain a milky-white colloid, (ii) adding an alkaline solution of pH 8 to pH 12, containing more than 1 mol of salts, such as monovalent salts, to the milky-white colloid, to separate DNA from protamines, and then (iii) adding ethanol solution to the resultant mixture, effecting a precipitation of DNA.

摘要翻译： 从鱼类精原细菌获得DNA的方法包括：（i）破碎鱼类精母细胞以获得乳白色胶体，（ii）将pH8的碱性溶液加入pH12，含有多于1摩尔的盐，如一价盐， 向乳白色胶体中分离DNA，然后（iii）向所得混合物中加入乙醇溶液，进行DNA的沉淀。

公开(公告)号：US20240316631A1

公开(公告)日：2024-09-26

申请号：US18577866

申请日：2022-07-20

申请人： BIONEER CORPORATION

发明人： Han-Oh PARK ,  Jae-Ha KIM ,  jun Pyo KIM ,  Kug Jin YUN

IPC分类号： B22F1/17 ,  B22F1/054 ,  B22F1/10 ,  C08K9/02 ,  C09D5/24 ,  C09D7/40 ,  C09D7/62

CPC分类号： B22F1/17 ,  B22F1/0547 ,  B22F1/056 ,  B22F1/10 ,  C09D5/24 ,  C09D7/62 ,  C09D7/68 ,  C09D7/70 ,  B22F2301/10 ,  B22F2301/255 ,  B22F2304/058 ,  C08K9/02 ,  C08K2201/001 ,  C08K2201/003 ,  C08K2201/011 ,  C08K2201/016

摘要： The present invention relates to a metal nanowire having a core-shell structure, the metal nanowire being conductive and transparent while having excellent oxidation stability, and being capable of maintaining excellent oxidation stability even after a secondary process using the metal nanowire of the present invention. Specifically, the metal nanowire having a core-shell structure, according to the present invention, comprises a core containing copper, and a shell containing silver on the core, wherein the ratio (D/L) of the diameter (D) of the core to the thickness (L) of the shell is 10-60, the thickness of the shell is 5-40 nm, and the peak intensity (I1) of Ag 3d5/2 of silver and the peak intensity (I2) of Cu 2p3/2 of copper in the X-ray photoelectron spectrum satisfy the following Equation 1.









I
2

/

I
1


≤

0
.
2





(

Equation
⁢

1

)

公开(公告)号：US20240299437A1

公开(公告)日：2024-09-12

申请号：US18261882

申请日：2022-02-25

申请人： BIONEER CORPORATION ,  SIRNAGEN THERAPEUTICS CORPORATION

发明人： HAN OH PARK ,  SANG KYU LEE ,  OH SEUNG KWON ,  EUN AH GOH

IPC分类号： A61K31/7105 ,  A61K8/60 ,  A61K9/51 ,  A61K31/713 ,  A61K47/54 ,  A61K47/58 ,  A61K47/60 ,  A61K47/69 ,  A61P17/00 ,  A61Q5/00 ,  A61Q7/00 ,  C12N15/113

CPC分类号： A61K31/7105 ,  A61K8/606 ,  A61K9/51 ,  A61K31/713 ,  A61K47/543 ,  A61K47/554 ,  A61K47/58 ,  A61K47/60 ,  A61K47/6929 ,  A61P17/00 ,  A61Q5/00 ,  A61Q7/00 ,  C12N15/113

摘要： The present invention relates to a composition for alleviating hair graying, promoting hair growth and/or preventing or alleviating hair loss, comprising double-stranded miRNA as an active ingredient and, more specifically, to: miR-3139, miR-3189, miR-3199 or miR-8485, which are double-stranded miRNA; a double-stranded oligonucleotide structure comprising same; or a pharmaceutical or cosmetic composition for alleviating hair graying, promoting hair growth and/or preventing or alleviating hair loss, comprising nanoparticles containing the structure as an active ingredient. A composition according to the present invention can prevent hair graying and reduce the rate of progress thereof by activating melanocytes and promoting melanogenesis and can allow the hair already affected by graying to be improved to a state before graying. In addition, the composition promotes the activation of melanocytes and the proliferation of dermal papilla cells and keratinocytes that are present in hair follicles, and induces outer root sheath development and hair growth so that the effects of promoting hair growth and/or preventing hair loss can be exhibited.

公开(公告)号：US20240076672A1

公开(公告)日：2024-03-07

申请号：US18478014

申请日：2023-09-29

申请人： BIONEER CORPORATION

发明人： Taewoo LEE ,  Jiwon RYU ,  Eun Ji IM

IPC分类号： C12N15/113 ,  A61P35/00

CPC分类号： C12N15/1136 ,  A61P35/00 ,  C12N2310/141 ,  C12N2310/50

摘要： The present invention relates to a double-helix oligonucleotide construct comprising a double-stranded miRNA and a composition for preventing or treating cancer comprising the same. More particularly, the present invention relates to a double-helix oligonucleotide construct comprising miR-544a characterized by a method that effectively inhibits the proliferation of cancer cells or induces a voluntary death of cancer cells, and an anticancer composition comprising the construct.

公开(公告)号：US20240002833A1

公开(公告)日：2024-01-04

申请号：US18253582

申请日：2021-11-30

申请人： BIONEER CORPORATION

发明人： Han-Oh PARK ,  Myung ll KIM ,  Sang Ryoung PARK ,  Na Young LEE ,  Geon Woo GU

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C07H1/06 ,  B03C1/01 ,  B03C1/28

CPC分类号： C12N15/1013 ,  C12Q1/6806 ,  C07H1/06 ,  B03C1/01 ,  B03C1/28 ,  B03C2201/18 ,  B03C2201/26

摘要： The present invention relates to a method for isolating a nucleic acid by using: a binding buffer containing acetic acid or phosphoric acid; and the pH difference between binding and washing buffers, and an elution buffer, wherein silica magnetic particles are added to a biological sample including a nucleic acid to which a binding buffer containing acetic acid, phosphoric acid, etc., has been added, thereby binding the nucleic acid to the magnetic particles, after which a magnetic field is applied to separate the magnetic particles, and the nucleic acid is purified in a basic pH elution buffer. The method uses the pH difference, and can isolate the nucleic acid more quickly and efficiently than centrifugation or gravity separation. In addition, since ethanol is not used, high-purity nucleic acid can be isolated and purified to obtain more accurate results in subsequent experiments.

公开(公告)号：US20230372945A1

公开(公告)日：2023-11-23

申请号：US18247614

申请日：2021-10-05

申请人： BIONEER CORPORATION

发明人： Han Oh PARK ,  Hanee PARK ,  JIN IL LEE ,  Jong Kab KIM ,  Eun Yeong KWON

IPC分类号： B01L7/00 ,  B01L3/02 ,  B01L3/00

CPC分类号： B01L7/52 ,  B01L3/0234 ,  B01L3/5453 ,  B01L2200/0689 ,  B01L2300/021 ,  B01L2300/0627 ,  B01L2300/0829 ,  B01L2200/025

摘要： The present invention relates to a nucleic acid amplification test apparatus, and an automatic sample analysis system having same and, more particularly, to a nucleic acid amplification test apparatus in which separation and purification, dispensation, amplification, and testing of samples are performed integrally, and an automatic sample analysis system having same. Disclosed in the present invention is a nucleic acid amplification test apparatus comprising: a housing (30) having an inner space (S1) isolated from the outside; a multi-well plate insertion unit (100) into which a multi-well plate (40) having a plurality of reaction tubes (1) accommodating a target nucleic acid solution is inserted through an automatic purification and dispensing apparatus (10); a sealing plate insertion unit (200) into which a sealing plate (50) having a sealing means for sealing the inlets of the plurality of reaction tubes (1); a fluorescence detection unit (400) disposed on an upper side of the sealing plate insertion unit (200) and detecting a target nucleic acid in the reaction tubes (1); and a temperature control block (500) disposed on a lower side of the multi-well plate insertion unit (100) and controlling the temperature of the reaction tubes (1), wherein the reaction tubes (1) are moved relatively so as to be adjacent to the sealing means to be then sealed by the sealing means.

公开(公告)号：US20230203494A1

公开(公告)日：2023-06-29

申请号：US18067363

申请日：2022-12-16

申请人： BIONEER CORPORATION

发明人： Tae-Rim KIM ,  Pyoung Oh YOON ,  Youngho KO ,  Seon Joo BAE ,  Han-Oh PARK ,  Seung Seob SON ,  Jun-Hong PARK ,  Sung-II YUN

IPC分类号： C12N15/113 ,  A61P11/00 ,  A61K9/19 ,  A61K31/713

CPC分类号： C12N15/1136 ,  A61P11/00 ,  A61K9/19 ,  A61K31/713 ,  C12N2310/14 ,  C12N2310/314 ,  C12N2310/322 ,  C12N2310/351 ,  C12N2310/3515

摘要： The present invention relates to a double-stranded oligonucleotide which can highly specifically and efficiently inhibit an amphiregulin expression and, preferably, a double-stranded oligonucleotide comprising a sequence in the form of RNA/RNA, DNA/DNA or DNA/RNA hybrid, a double-stranded oligonucleotide structure comprising the double-stranded oligonucleotide, nanoparticles comprising the double-stranded oligonucleotide structure, and a fibrosis or respiratory disease preventive or therapeutic use thereof.

公开(公告)号：US20230168266A1

公开(公告)日：2023-06-01

申请号：US17996960

申请日：2021-04-19

申请人： BIONEER CORPORATION

发明人： Han Oh PARK ,  Jong-Kab KIM

IPC分类号： G01N35/10

CPC分类号： G01N35/1011 ,  G01N2035/00752

摘要： The present disclosure relates to a trackable precise-sample-injection device for a multi-well plate and, more specifically, to a trackable precise-sample-injection device for a multi-well plate, the device being capable of: preventing cross-contamination that can occur when many samples are manually put into a multi-well plate, and a diagnosis error caused by the injection of a sample into the wrong well or repeated injection; and simply recording and tracking information about wells into which samples are injected. The present disclosure relates to a trackable precise-sample-injection device for a multi-well plate, the device being capable of being efficiently used for a pooling inspection, and the like by injecting a plurality of samples into a multi-well plate.