公开(公告)号：US12188068B2

公开(公告)日：2025-01-07

申请号：US17229543

申请日：2021-04-13

Applicant: Genomatica, Inc.

Inventor： Stephen B. Del Cardayre ,  Andreas W. Schirmer ,  Myong Ko ,  Haibo Wang

IPC: C12P17/08 ,  C12P7/42 ,  C12P7/44 ,  C12P17/04

Abstract: The present disclosure relates to chemo-enzymatic processes for the preparation of lactones (including, e.g., macrolactones, γ-lactones, and δ-lactones) and/or macrocyclic ketones, which are compounds of industrial value, for example, for use as fragrance ingredients. The chemo-enzymatic processes combine the in vivo microbial production of fatty acid derivatives and the subsequent ex vivo synthetic transformation of the fatty acid derivatives to provide the lactones and macrocyclic ketones.

公开(公告)号：US12157904B2

公开(公告)日：2024-12-03

申请号：US17715587

申请日：2022-04-07

Applicant: Genomatica, Inc.

Inventor： Angelica Zabala Bautista ,  Min Sun Park ,  Alma Itzel Ramos-Solis ,  Bernardo Moura Torres Da Costa ,  Behnaz Behrouzian ,  Donald E. Trimbur ,  Emanuela Popova ,  Qijun Hu ,  Stephen Bralley Del Cardayre

IPC: C12N9/16 ,  C12P7/6409 ,  C12P7/6436

Abstract: The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.

公开(公告)号：US20240218346A1

公开(公告)日：2024-07-04

申请号：US18554448

申请日：2022-04-08

Applicant: Genomatica, Inc.

Inventor： Hui ZHOU ,  Nathan SCHMIDT ,  Pichet PRAVESCHOTINUNT ,  Brian CARVALHO

IPC: C12N9/88 ,  C12N15/63 ,  C12P1/00

CPC classification number: C12N9/88 ,  C12N15/63 ,  C12P1/00 ,  C12Y401/02009

Abstract: The disclosure provides polypeptides and encoding nucleic acids of engineered phosphoketolases. The disclosure also provides cells expressing an engineered phosphoketolase. The disclosure further provides methods for producing a bioderived compound comprising culturing cells expressing an engineered phosphoketolase.

公开(公告)号：US20240182935A1

公开(公告)日：2024-06-06

申请号：US18321585

申请日：2023-05-22

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Connor J. Galleher ,  Michael Japs ,  Mark J. Burk ,  Cara Tracewell

IPC: C12P13/00 ,  C12N1/02 ,  C12N1/20 ,  C12N9/88 ,  C12N9/96 ,  C12N11/16 ,  C12N15/52

CPC classification number: C12P13/001 ,  C12N1/02 ,  C12N1/20 ,  C12N9/88 ,  C12N9/96 ,  C12N11/16 ,  C12N15/52 ,  C12Y402/01001

Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

公开(公告)号：US20240158326A1

公开(公告)日：2024-05-16

申请号：US18334745

申请日：2023-06-14

Applicant: Genomatica, Inc.

Inventor： Myong KO ,  Haibo Wang ,  Patricia J. Cole ,  Perry Y. Liao ,  Simon Li

IPC: C07C29/88 ,  C07C29/17

CPC classification number: C07C29/88 ,  C07C29/172

Abstract: The disclosure relates to downstream processing of fatty alcohol (FALC) and provides a novel purification method that provides FALC at high purity and yield.

公开(公告)号：US20240150798A1

公开(公告)日：2024-05-09

申请号：US18482834

申请日：2023-10-06

Applicant: Genomatica, Inc.

Inventor： Benjamin ADELSTEIN ,  Robin E. OSTERHOUT ,  Mark J. BURK

IPC: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C12N9/18 ,  C12N15/52

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/011 ,  C12Y301/01067 ,  C12Y301/08001 ,  C07B2200/05 ,  C12R2001/00 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.

公开(公告)号：US20240141397A1

公开(公告)日：2024-05-02

申请号：US18242912

申请日：2023-09-06

Applicant: Genomatica, Inc.

Inventor： Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N9/00 ,  C12N9/02 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N9/16 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P17/08

CPC classification number: C12P17/10 ,  C12N9/0004 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/0016 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/13 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/18 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P17/08 ,  C12Y101/01027 ,  C12Y101/0103 ,  C12Y101/01035 ,  C12Y101/01037 ,  C12Y101/01172 ,  C12Y103/01031 ,  C12Y104/01009 ,  C12Y203/01174 ,  C12Y206/01019 ,  C12Y208/03006 ,  C12Y301/02004 ,  C12Y603/03004

Abstract: Provided herein are non-naturally occurring microbial organisms having biosynthetic pathways for production of target products and one or more genetic modifications that reduce a byproduct of the biosynthetic pathway. Compositions of target products from such cells and methods of using such cells are provided.

公开(公告)号：US20240052379A1

公开(公告)日：2024-02-15

申请号：US18346715

申请日：2023-07-03

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C07D207/12 ,  C08G18/32 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02

CPC classification number: C12P7/18 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y101/01244 ,  C12Y101/02007 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

公开(公告)号：US20230416698A1

公开(公告)日：2023-12-28

申请号：US18191774

申请日：2023-03-28

Applicant: Genomatica, Inc.

Inventor： Amit Shah ,  Joseph Warner

IPC: C12N9/02 ,  C12N15/90 ,  C12P7/04 ,  C12P7/62 ,  C12P13/02

CPC classification number: C12N9/0008 ,  C12N15/90 ,  C12P7/04 ,  C12P7/62 ,  C12P13/02 ,  C12Y102/01003

Abstract: The invention provides polypeptides and encoding nucleic acids of aldehyde dehydrogenase variants. The invention also provides cells expressing aldehyde dehydrogenase variants. The invention further provides methods for producing 3-hydroxybutyraldehyde (3-HBal) and/or 1,3-butanediol (1,3-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells. The invention additional provides methods for producing 4-hydroxybutyraldehyde (4-HBal) and/or 1,4-butanediol (1,4-BDO), or an ester or amide thereof, comprising culturing cells expressing an aldehyde dehydrogenase variant or using lysates of such cells.

公开(公告)号：US11814664B2

公开(公告)日：2023-11-14

申请号：US14893510

申请日：2014-05-23

Applicant: Genomatica, Inc.

Inventor： Benjamin Adelstein ,  Robin E. Osterhout ,  Mark J. Burk

IPC: C12P7/62 ,  C12N9/18 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  A23L33/135 ,  C12N1/00 ,  C12N1/14 ,  C12N1/20 ,  C07C69/675 ,  C12N15/52 ,  C12R1/00 ,  C12R1/01 ,  C12R1/645

CPC classification number: C12P7/62 ,  A23L33/135 ,  A61K31/22 ,  A61K35/66 ,  A61K36/064 ,  C07C69/675 ,  C12N1/00 ,  C12N1/145 ,  C12N1/205 ,  C12N9/18 ,  C12N15/52 ,  C12Y101/011 ,  C12Y101/01035 ,  C12Y301/01067 ,  C12Y301/08001 ,  A23V2002/00 ,  C07B2200/05 ,  C12R2001/00 ,  C12R2001/01 ,  C12R2001/645 ,  Y02P20/10

Abstract: Provided herein are non-naturally occurring microbial organisms having a pathway for production of (3R)-hydroxybutyl (3R)-hydroxybutyrate, wherein the organism can further include a (R)-1,3-butanediol pathway, a (3R)-hydroxybutyrate pathway, a (3R)-hydroxybutyryl-CoA pathway, an acetoacetate pathway, an acetoacetyl-CoA pathway, a (3R)-hydroxybutyl-ACP pathway, or an acetoacetyl-ACP pathway. Additionally provided are methods and processes for producing and isolating (3R)-hydroxybutyl (3R)-hydroxybutyrate using the microbial organisms, and various compositions having the (3R)-hydroxybutyl (3R)-hydroxybutyrate. Still further provided are methods of treating or preventing a disease, disorder or condition using the (3R)-hydroxybutyl (3R)-hydroxybutyrate produced by the microbial organisms of the invention.