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145 条记录 (耗时 0.062 秒)

    MULTI-CHANNEL CAPILLARY ELECTROPHORESIS MICROCHIPS AND USES THEREOF
    71.
    发明申请
    MULTI-CHANNEL CAPILLARY ELECTROPHORESIS MICROCHIPS AND USES THEREOF 审中-公开
    多通道毛细管电泳细胞及其用途

    公开(公告)号：US20090127114A1

    公开(公告)日：2009-05-21

    申请号：US12158489

    申请日：2006-12-22

    申请人： Min Guo ,  Guibin Du ,  Guangsheng Gu ,  Wanli Xing ,  Jing Cheng

    发明人： Min Guo ,  Guibin Du ,  Guangsheng Gu ,  Wanli Xing ,  Jing Cheng

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44791

    摘要： A multi-channel capillary electrophoresis microchip for analysis of multiple samples comprising: at least two sample reservoirs, at least two sample channels corresponding to said sample reservoirs, a sample buffer reservoir, a sample waste reservoir, a separation buffer reservoir, a separation waste reservoir, a sample loading channel, and a separation channel, wherein each of said sample channels is connected at one end to said sample loading channel at a place between said sample buffer reservoir and said intersection of said sample loading channel and said separation channel, and the other end of each of said sample channels is connected to each of said sample reservoirs. A method of capillary electrophoresis separation for sequentially analysis of multiple samples.

    摘要翻译： 一种用于分析多个样品的多通道毛细管电泳微芯片，包括：至少两个样品储存器，对应于所述样品储存器的至少两个样品通道，样品缓冲液储存器，样品废物储存器，分离缓冲液储存器，分离废液储存器 ，样品加载通道和分离通道，其中每个所述样品通道在一端连接到所述样品缓冲液储存器和所述样品加载通道与所述分离通道的交叉点之间的位置处的所述样品加载通道，并且 每个所述样品通道的另一端连接到每个所述样品储存器。 毛细管电泳分离方法，用于顺序分析多个样品。

    Optical Systems for Microarray Scanning
    73.
    发明申请
    Optical Systems for Microarray Scanning 有权
    微阵列扫描光学系统

    公开(公告)号：US20090046756A1

    公开(公告)日：2009-02-19

    申请号：US11795653

    申请日：2005-06-03

    申请人： Xianhua Wang ,  Guoliang Huang ,  Jing Cheng ,  Hong Zhang ,  Taishan Gao

    发明人： Xianhua Wang ,  Guoliang Huang ,  Jing Cheng ,  Hong Zhang ,  Taishan Gao

    IPC分类号： H01S3/08 ,  H01S3/10

    CPC分类号： G02B21/002 ,  G01N21/6452

    摘要： The invention provides a novel optical system for use in a microarray scanner, comprising an aperture-containing reflecting mirror comprising an aperture and a reflecting surface. The aperture of the aperture-containing reflecting mirror allows an excitation light to pass through, and the reflecting surface of the aperture-containing reflecting mirror allows emission light from a microarray to be reflected. The optical system may also comprises various other components such as laser generators, beam splitter, reflecting mirrors, excitation and emission light filters, excitation and emission objective lens, pinhole, and detector. The optical system described herein has high efficiency, high sensitivity, low background noise, structurally simple, and high versatility.

    摘要翻译： 本发明提供了一种用于微阵列扫描仪的新型光学系统，其包括一个包含孔和反射表面的孔径反射镜。 含孔反射镜的孔径允许激发光通过，并且含孔反射镜的反射表面允许来自微阵列的发射光被反射。 光学系统还可以包括诸如激光发生器，分束器，反射镜，激发和发射滤光器，激发和发射物镜，针孔和检测器的各种其它部件。 本文描述的光学系统具有高效率，高灵敏度，低背景噪声，结构简单和高通用性。

    Methods, compositions, and automated systems for separating rare cells from fluid samples
    77.
    发明授权
    Methods, compositions, and automated systems for separating rare cells from fluid samples 有权

    公开(公告)号：US06949355B2

    公开(公告)日：2005-09-27

    申请号：US10268312

    申请日：2002-10-10

    申请人： Douglas Yamanishi ,  Paul Hujsak ,  Zhaohuai Yang ,  J{dot over (u)}nquan Xu ,  Xiaobo Wang ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Jing Cheng

    发明人： Douglas Yamanishi ,  Paul Hujsak ,  Zhaohuai Yang ,  J{dot over (u)}nquan Xu ,  Xiaobo Wang ,  Lei Wu ,  Mingxian Huang ,  Guoliang Tao ,  Jing Cheng

    IPC分类号： A61M1/36 ,  C12N5/00 ,  C12N5/06 ,  C12N5/08 ,  G01N1/40 ,  G01N15/02 ,  G01N15/05 ,  G01N15/06 ,  C12Q1/04

    CPC分类号： G01N15/05 ,  A61M1/36 ,  A61M1/3618 ,  A61M1/362 ,  A61M1/3679 ,  G01N1/40 ,  G01N15/0272 ,  G01N33/491 ,  G01N2015/0288 ,  G01N2015/0294 ,  G01N2015/0681

    摘要： The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells from a complex fluid sample. In particular, the enrichment of fetal cells from maternal samples, such as maternal blood samples, can greatly aid in the detection of fetal abnormalities or a variety of genetic conditions. In addition, the present invention recognizes that the enrichment of rare malignant cells from patient samples, can aid in diagnosis, prognosis, and development of therapeutic modalities for patients.A first aspect of the present invention is a microfabricated filter for filtering a fluid sample. A microfabricated filter of the present invention comprises at least one tapered pore, and preferably comprises at least two tapered pores whose variation in size is 20% or less. The present invention also includes a method of enriching rare cells of a fluid sample using a microfabricated filter of the present invention.Another aspect of the invention is solutions for the selective sedimentation of red blood cells (RBCs) from a blood sample comprising a red blood cell aggregating agent and at least one specific binding member that selectively binds RBCs. Solutions of the present invention include a combined solution for rare cell enrichment that comprise RBC aggregating agents, at least one specific binding member that selectively binds RBCs, and at least one additional specific binding member for the removal of undesirable sample components other than RBCs. The invention also includes methods of using selective RBC sedimentation solutions and combined solutions for enriching rare cells of a fluid sample.Yet another aspect of the invention is an automated system for processing a fluid sample that includes: at least one filtration chamber that comprises or engages one or more microfabricated filters of the present invention; automated means for directing fluid flow through the one or more filtration chambers of the automated system, and means for collecting enriched rare cells. The present invention also includes methods of using an automated system for separating rare cells from a fluid sample. Preferred fluid samples are effusion, blood, or urine samples, and rare cells that can be enriched from such sample include nucleated red blood cells and cancer cells.

    Capillary electrophoresis chip apparatus for detecting polymorphism of nucleotide and mononucleotide
    78.
    发明申请
    Capillary electrophoresis chip apparatus for detecting polymorphism of nucleotide and mononucleotide 有权
    用于检测核苷酸和单核苷酸多态性的毛细管电泳切片装置

    公开(公告)号：US20050161335A1

    公开(公告)日：2005-07-28

    申请号：US10500180

    申请日：2002-11-29

    申请人： Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

    发明人： Peng Liu ,  Wanli Xing ,  Dong Liang ,  Jing Cheng

    IPC分类号： G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44791

    摘要： The present invention provides a capillary electrophoresis chip apparatus for detecting nucleotide polymorphism or single nucleotide polymorphism belonging to capillary electrophoresis apparatuses. The apparatus comprises an upper channel layer comprising a one-, two-, or multi-dimensional microfluid channel and an electrode aperture structure for loading sample, a middle electrode layer for sealing the microfluid channel to form an intact capillary and providing the needed voltage for electrophoresis; and a lower heating layer for providing a stable temperature gradient for electrophoresis. The upper, middle and lower layers are thermal conductive and adhesive to each other.

    摘要翻译： 本发明提供一种用于检测属于毛细管电泳装置的核苷酸多态性或单核苷酸多态性的毛细管电泳芯片装置。 该装置包括上通道层，其包括一维，二维或多维微流体通道和用于装载样品的电极孔结构，用于密封微流体通道以形成完整毛细管的中间电极层，并提供所需电压 电泳; 以及用于提供电泳的稳定温度梯度的较低加热层。 上，中，下层相互导热，粘合。