公开(公告)号：US5599453A

公开(公告)日：1997-02-04

申请号：US471541

申请日：1995-06-06

Applicant: Pierre Girot ,  Egisto Boschetti

Inventor： Pierre Girot ,  Egisto Boschetti

IPC: B01D15/08 ,  B01J20/32 ,  B01J39/16

CPC classification number: B01D15/20 ,  B01J20/08 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/288 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/327 ,  B01J20/3282 ,  B01J20/3289 ,  B01J39/165 ,  B01D15/363 ,  B01D15/3804 ,  B01J2220/54 ,  B01J2220/58

Abstract: This invention relates generally to modified porous solid supports and processes for the preparation and use of same. In particular, passivated porous mineral oxide supports are disclosed which are characterized by a reversible high sorptive capacity substantially unaccompanied by non-specific adsorption of or interaction with biomolecules. Passivation is achieved by use of a passivation mixture comprising a main monomer, a passivating monomer and a crosslinking agent, which mixture upon polymerization results in the substantial elimination of the undesirable non-specific interaction with biomolecules.

Abstract translation: 本发明一般涉及改性多孔固体载体及其制备和使用方法。 特别地，公开了钝化的多孔矿物氧化物载体，其特征在于具有可逆的高吸附能力，其基本上不伴随生物分子的非特异性吸附或相互作用。 通过使用包含主单体，钝化单体和交联剂的钝化混合物实现钝化，该聚合物上的该混合物导致基本上消除了与生物分子的不期望的非特异性相互作用。

公开(公告)号：US4478713A

公开(公告)日：1984-10-23

申请号：US535308

申请日：1983-09-23

Applicant: Pierre Girot ,  Egisto Boschetti

Inventor： Pierre Girot ,  Egisto Boschetti

IPC: B01D15/08 ,  G01N30/24 ,  G01N30/82

CPC classification number: B01D15/08 ,  B01D15/14 ,  B01D15/247 ,  G01N30/24 ,  G01N30/82

Abstract: In the automatic installation for liquid chromatography according to the invention, the container of product to be fractionated is associated to a measuring container and is provided, as are the containers of eluents, with low level detector means; feed valves are interposed between said containers and the injection pump whereas output valves are interposed between the outlet from the column and the collector containers; a control unit comprises a safety device connected to the low level detectors to control the stoppage of the pump whenever necessary, and a control device receiving the signals from detection means connected to the output of the column and controlling the measuring container and said valves to perform successive cycles of chromatography each one including the steps of injecting a quantity of product, admitting at least one eluent and collecting the or each corresponding fraction.

Abstract translation: 在根据本发明的液相色谱的自动安装中，要分级的产品的容器与测量容器相关联，并且与洗涤液的容器一样设置有低水平检测器装置; 进料阀介于所述容器和注射泵之间，而输出阀插入在塔的出口和收集容器之间; 控制单元包括连接到低电平检测器的安全装置，以在必要时控制泵的停止;以及控制装置，其接收来自连接到列的输出的检测装置的信号，并控制测量容器和所述阀执行 连续的色谱层析每个包括以下步骤：注入一定数量的产物，允许至少一种洗脱剂并收集或相应的部分。

公开(公告)号：US4048377A

公开(公告)日：1977-09-13

申请号：US648084

申请日：1976-01-12

Applicant: Egisto Boschetti ,  Yvette Paule Nicole Moroux ,  Rene Tixier

Inventor： Egisto Boschetti ,  Yvette Paule Nicole Moroux ,  Rene Tixier

IPC: C08L5/12 ,  G01N27/447 ,  B32B27/08 ,  C08L5/00

CPC classification number: G01N27/44747 ,  C08L5/12 ,  Y10T428/31725 ,  Y10T428/31736 ,  Y10T428/31797

Abstract: Dried agarose or gelose-containing films which are rehydratable into aqueous gel films are disclosed. The dried films are prepared by first forming, on a support, an aqueous gel film containing at most 5% by weight of agarose or gelose and a water-soluble linear polymer or copolymer of acrylamide or methacrylamide. The viscosity of such polymer and copolymer in a 5% aqueous solution at 22.degree. C is about 17000 centipoises or less and preferably about 6000 centipoises or less. The aqueous gel film is then dried according to known techniques, with the linear polymer or copolymer of acrylamide or methacrylamide being included in the dried film.

Abstract translation: 公开了可再水化成水性凝胶膜的干琼脂糖或含有糖的胶囊。 干燥的膜通过首先在载体上形成含有至多5重量％的琼脂糖或谷蛋白的水凝胶膜和丙烯酰胺或甲基丙烯酰胺的水溶性线性聚合物或共聚物来制备。 这种聚合物和共聚物在22℃的5％水溶液中的粘度为约17000厘泊或更低，优选约6000厘泊或更小。 然后根据已知技术干燥水性凝胶膜，其中包括丙烯酰胺或甲基丙烯酰胺的直链聚合物或共聚物。

公开(公告)号：US08491791B2

公开(公告)日：2013-07-23

申请号：US13552356

申请日：2012-07-18

Applicant: Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

Inventor： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

IPC: B01D63/00 ,  B01D61/00 ,  B01D15/08 ,  G01N1/00

CPC classification number: G01N30/461 ,  B01D15/325 ,  B01D15/362 ,  B01D15/363 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0487 ,  C07K1/34 ,  G01N27/44704 ,  G01N27/44795 ,  G01N30/466 ,  G01N30/468 ,  Y10T137/2224 ,  Y10T137/794 ,  Y10T137/86187 ,  Y10T436/255

Abstract: The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.

Abstract translation: 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

公开(公告)号：US08246832B2

公开(公告)日：2012-08-21

申请号：US11915151

申请日：2006-01-19

Applicant: Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

Inventor： Lee O. Lomas ,  Jian Ding ,  Egisto Boschetti

IPC: B01D63/00

CPC classification number: G01N30/461 ,  B01D15/325 ,  B01D15/362 ,  B01D15/363 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/563 ,  B01L2200/026 ,  B01L2200/028 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2400/0487 ,  C07K1/34 ,  G01N27/44704 ,  G01N27/44795 ,  G01N30/466 ,  G01N30/468 ,  Y10T137/2224 ,  Y10T137/794 ,  Y10T137/86187 ,  Y10T436/255

Abstract: The present invention contemplates various devices that are configured to separate a sample, which contains more than one unique species, into any desired number of sub-samples by passing the sample across a like number of separation media configured for a first separation protocol. Each of the sub-samples may be further separated by an additional separation protocol, thereby creating a plurality of mini-samples, each of which may be further separated and/or analyzed. The invention also contemplates using a simple method of using conduits to form a fluid path that passes through a plurality of separation media, each of which media is configured to isolate a particular sub-sample. After various sub-samples of the sample are isolated by the various separation media, the conduits may be removed, thereby enabling each of the isolated sub-samples to be further separated and/or analyzed independent of any other sub-sample.

Abstract translation: 本发明考虑了通过将样品穿过配置用于第一分离方案的相似数量的分离介质，将被配置成将含有多于一种独特物种的样品分离成任何所需数量的子样品的各种装置。 每个子样品可以通过另外的分离方案进一步分离，从而产生多个微型样品，每个小样品可进一步分离和/或分析。 本发明还考虑使用简单的方法来使用导管来形成通过多个分离介质的流体路径，其中每个介质被配置为隔离特定的子样品。 在通过各种分离介质分离样品的各种亚样品之后，可以去除导管，从而使每个分离的子样品能够独立于任何其它亚样品进一步分离和/或分析。

公开(公告)号：US20110313147A1

公开(公告)日：2011-12-22

申请号：US13210568

申请日：2011-08-16

Applicant: Egisto Boschetti ,  Pierre Girot

Inventor： Egisto Boschetti ,  Pierre Girot

IPC: C08B15/00 ,  C07C217/06 ,  C07F7/00 ,  C07C323/16 ,  C07C323/12 ,  C07C323/25 ,  C07C323/52

CPC classification number: B01J39/26 ,  B01D15/327 ,  B01D15/361 ,  B01D15/362 ,  B01J20/289 ,  B01J20/3242 ,  B01J20/3285 ,  B01J41/20 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00702 ,  B01J2219/0074 ,  B01J2220/54 ,  C07K1/16 ,  C07K1/18 ,  G01N30/02 ,  G01N33/54353 ,  G01N2035/00158 ,  Y10S435/803

Abstract: Ion exchange and hydrophobic interaction chromatographic materials are constructed by tethering a terminal binding functionality to a solid support via a hydrophobic linker. The backbone of the linker typically comprises sulfur-containing moieties. Suitable terminal binding functionalities are tertiary amines, quaternary ammonium salts, or hydrophobic groups. These chromatographic materials possess both hydrophobic and ionic character under the conditions prescribed for their use. The separation of proteins from crude mixtures at physiological ionic strength can be accomplished with a chromatographic material of this type by applying pH or ionic strength gradients, thereby effecting protein adsorption and desorption.

Abstract translation: 离子交换和疏水相互作用层析材料是通过一个疏水性接头将末端结合官能团连接到固体支持物构成的。 接头的骨架通常包含含硫部分。 合适的末端结合官能团是叔胺，季铵盐或疏水基团。 这些色谱材料在规定的条件下具有疏水性和离子性。 通过施加pH或离子强度梯度，可以用这种类型的色谱材料在生理离子强度下从粗混合物中分离蛋白质，从而进行蛋白质吸附和解吸。

公开(公告)号：US07754861B2

公开(公告)日：2010-07-13

申请号：US11388181

申请日：2006-03-22

Applicant: Egisto Boschetti ,  Lee O. Lomas

Inventor： Egisto Boschetti ,  Lee O. Lomas

IPC: A23J1/00 ,  C07K14/00 ,  C07K17/00 ,  C40B40/10

CPC classification number: C07K1/047 ,  C07K1/22

Abstract: The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moieties having different binding moieties, binding the contaminating proteins and a minority of the target protein group to the library of binding moieties, separating the unbound target protein group from the proteins bound to the library of binding moieties and collecting the unbound target protein. The collected target protein is more pure than the target protein group in the sample.

Abstract translation: 本发明提供了用于纯化靶蛋白质组的方法和试剂盒。 该方法包括以下步骤：将包含至少95％的目标蛋白质组和至多5％的污染蛋白质的样品与具有不同结合部分的结合部分的文库结合，结合污染蛋白质和少数靶蛋白质组 到结合部分的文库，将未结合的靶蛋白基团与结合到结合部分文库的蛋白质分离并收集未结合的靶蛋白质。 收集的目标蛋白质比样品中的目标蛋白质组更为纯净。

公开(公告)号：US20100173791A1

公开(公告)日：2010-07-08

申请号：US12651942

申请日：2010-01-04

Applicant: Egisto Boschetti

Inventor： Egisto Boschetti

IPC: C40B30/02 ,  C40B50/02 ,  C40B40/00

CPC classification number: G01N33/6848 ,  C07K1/16 ,  G01N30/8675 ,  G01N2030/8831

Abstract: The present invention provides a facile and efficient method for determining a chromatographic protocol for separating a target protein from one or more second protein impurity. Also provided is a database facilitating the determination of an appropriate separation protocol.

Abstract translation: 本发明提供了一种用于确定用于从一种或多种第二种蛋白质杂质分离靶蛋白质的色谱方案的简便有效的方法。 还提供了便于确定适当分离方案的数据库。

公开(公告)号：US20080090737A1

公开(公告)日：2008-04-17

申请号：US11757260

申请日：2007-06-01

Applicant: Egisto Boschetti

Inventor： Egisto Boschetti

IPC: C40B30/04

CPC classification number: G01N33/6803 ,  C40B30/04 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/6845

Abstract: The present invention relates to the fields of molecular biology, combinatorial chemistry and biochemistry. Particularly, the present invention describes methods and kits for dynamically reducing the variance between analyte taken from complex mixtures.

Abstract translation: 本发明涉及分子生物学，组合化学和生物化学领域。 特别地，本发明描述了用于动态地减少从复杂混合物中取出的分析物之间的方差的方法和试剂盒。

公开(公告)号：US20070082019A1

公开(公告)日：2007-04-12

申请号：US10546173

申请日：2004-02-20

Applicant: Wenxi Huang ,  Yury Agroskin ,  Egisto Boschetti

Inventor： Wenxi Huang ,  Yury Agroskin ,  Egisto Boschetti

IPC: A61F2/02 ,  C12M1/34

CPC classification number: H01J49/0418 ,  A61L27/52

Abstract: A hydrogel layer is applied to a substrate advantageously when the layer is formed in situ, using a polymeric or copolymeric precursor that includes, respectively, monomer subunits that have a photocrosslinkable functionality and monomer subunits that have a chemically selective functionality for binding a biomolecular analyte, such as a protein. A hydrogel-coated substrate thus obtained is particularly useful as a probe for mass spectroscopic analysis, including SELDI analysis. Hydrogel particles also can be used for SELDI analysis.

Abstract translation: 使用聚合物或共聚物前体分别形成具有光可交联官能团的单体亚单位和具有化学选择性功能性用于结合生物分子分析物的单体亚单位，当层原位形成时，水凝胶层有利地施加到基底上， 如蛋白质。 由此获得的水凝胶涂覆的基底特别可用作用于质谱分析的探针，包括SELDI分析。 水凝胶颗粒也可用于SELDI分析。