公开(公告)号：US20080160630A1

公开(公告)日：2008-07-03

申请号：US11647565

申请日：2006-12-29

申请人： David J. Liu ,  Kai Wu ,  Xing Su

发明人： David J. Liu ,  Kai Wu ,  Xing Su

IPC分类号： G01N21/00 ,  G01N1/00

CPC分类号： G01N21/658 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2300/0654 ,  B01L2400/043 ,  B01L2400/086 ,  B82Y15/00 ,  B82Y30/00 ,  G01N21/255 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/76 ,  G01N33/54333 ,  G01N33/587

摘要： An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

摘要翻译： 本发明的一个实施例涉及用于检测样品中的分析物的装置。 该装置包括流体网络和集成电路部件。 流体网络包括样品区，清洁区和检测区。 流体网络包含磁性粒子和/或信号粒子。 引入含有分析物的样品，并且分析物通过亲和试剂与磁性颗粒和/或信号颗粒相互作用。 微线圈阵列或机械可移动永磁体在功能上耦合到流体网络，流体网络可激活以在流体网络的一部分内产生磁场，并将磁性颗粒从样品区域移动到检测区域。 存在检测元件，其检测来自信号颗粒的光学或电信号，从而指示分析物的存在。

公开(公告)号：US20070218478A1

公开(公告)日：2007-09-20

申请号：US11612454

申请日：2006-12-18

申请人： Qing Bai ,  Susana Salceda ,  Kai Wu ,  Thong Nguyen ,  Charles Miyada

发明人： Qing Bai ,  Susana Salceda ,  Kai Wu ,  Thong Nguyen ,  Charles Miyada

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6806 ,  C12Q2521/531 ,  C12Q2521/301 ,  C12Q2521/131

摘要： Methods for fragmenting and labeling DNA in a single reaction volume and incubation step using a uracil DNA glycosylase, an apurinic/apyrimidinic endonuclease, and a terminal transferase are disclosed. In a preferred embodiment the UDG, AP and TdT activities are first mixed together to form an enzyme mixture and then the enzyme mixture is mixed with the uracil containing DNA. The fragmentation and labeling reactions thus take place simultaneously as part of the same reaction. The methods may be used in a variety of applications where fragmenting and end-labeling single or double stranded DNA is desired.

摘要翻译： 公开了在单个反应体积中分离和标记DNA的方法以及使用尿嘧啶DNA糖基化酶，无嘌呤/脱嘧啶核苷酸内切酶和末端转移酶的温育步骤。 在优选的实施方案中，首先将UDG，AP和TdT活性混合在一起以形成酶混合物，然后将该酶混合物与含有尿嘧啶的DNA混合。 因此，碎裂和标记反应同时作为相同反应的一部分进行。 该方法可用于需要片段化和末端标记单链或双链DNA的多种应用。

公开(公告)号：US20070020654A1

公开(公告)日：2007-01-25

申请号：US11419459

申请日：2006-05-19

申请人： John Blume ,  Yanxiang Cao ,  Kyle Cole ,  Kai Wu ,  Charles Miyada

发明人： John Blume ,  Yanxiang Cao ,  Kyle Cole ,  Kai Wu ,  Charles Miyada

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C12Q1/6806 ,  C12Q2531/143 ,  C12Q2525/179 ,  C12Q2521/531 ,  C12Q2521/131

摘要： The present invention provides methods for preparing nucleic acid samples. The methods of the present invention are particularly amenable for preparing samples that substantially represent the whole transcripts. In some aspects the methods include a step of reducing the amount of ribosomal RNA in a total RNA sample prior to amplification. In preferred aspects single stranded sense strand cDNA is generated, labeled and hybridized to arrays of probes. The method is particularly suitable to use with microarray based expression analysis.

摘要翻译： 本发明提供了制备核酸样品的方法。 本发明的方法特别适于制备基本上代表整个转录物的样品。 在一些方面，所述方法包括在扩增前减少总RNA样品中的核糖体RNA的量的步骤。 在优选的方面，产生单链有义链cDNA，标记并与探针阵列杂交。 该方法特别适用于基于微阵列的表达分析。

公开(公告)号：US20060235937A1

公开(公告)日：2006-10-19

申请号：US11108285

申请日：2005-04-18

申请人： Kai Wu ,  Farzad Khosrowpour

发明人： Kai Wu ,  Farzad Khosrowpour

IPC分类号： G06F15/167

CPC分类号： G06F15/16

摘要： A system and method for receiving and responding to issued commands in a storage enclosure is disclosed in which each storage enclosure processor of the storage enclosure is coupled to each expander of the storage enclosure. Each storage enclosure processor receives each interrupt and command issued by another expander of the storage enclosure. In the event of a failure of one of the storage enclosure processors, any interrupts or commands issued by the expander associated with the failed storage enclosure processor will be handled by the operational storage enclosure processor. In this configuration, the storage enclosure processor can also arbitrate or determine the storage enclosure processor that will handle each interrupt and any associated command.

摘要翻译： 公开了一种用于接收和响应存储外壳中发布的命令的系统和方法，其中存储外壳的每个存储外壳处理器耦合到存储外壳的每个扩展器。 每个存储机箱​​处理器接收由存储机柜的另一个扩展器发出的每个中断和命令。 如果一个存储机箱​​处理器发生故障，则与故障存储机箱处理器相关联的扩展器发出的任何中断或命令将由操作存储机箱处理器处理。 在这种配置中，存储机箱处理器还可以仲裁或确定将处理每个中断的存储机箱处理器和任何相关联的命令。

公开(公告)号：US20060194243A1

公开(公告)日：2006-08-31

申请号：US11381813

申请日：2006-05-05

申请人： Rajeswari Tadakamalla ,  Kai Wu

发明人： Rajeswari Tadakamalla ,  Kai Wu

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： C12Q1/6809 ,  G16B25/00 ,  Y02A90/26 ,  C12Q2565/501 ,  C12Q2531/119 ,  C12Q2521/301

摘要： Methods and computer software products for identifying changes in genomic DNA copy number are disclosed. Methods for identifying homozygous deletions and genetic amplifications are disclosed. Genomic DNA is amplified generically and amplified sample is hybridized to an expression array. The expression array comprises probes to regions of genes that are expressed. The probes are complementary to genomic sequences found in mRNAs. Signal intensity is correlated to copy number. The methods may be used to detect copy number changes in cancerous tissue compared to normal tissue. The methods may be used to diagnose cancer and other diseases associated with chromosomal anomalies.

摘要翻译： 公开了用于鉴定基因组DNA拷贝数变化的方法和计算机软件产品。 公开了鉴定纯合缺失和遗传扩增的方法。 基因组DNA被放大一般，扩增的样品与表达数组杂交。 表达阵列包括对表达的基因区域的探针。 探针与mRNAs中发现的基因组序列互补。 信号强度与拷贝数相关。 与正常组织相比，该方法可用于检测癌组织中的拷贝数变化。 该方法可用于诊断与染色体异常相关的癌症和其他疾病。

公开(公告)号：US20060174085A1

公开(公告)日：2006-08-03

申请号：US11046599

申请日：2005-01-28

申请人： Kai Wu ,  Farzad Khosrowpour

发明人： Kai Wu ,  Farzad Khosrowpour

IPC分类号： G06F12/00

CPC分类号： G11B33/12 ,  G06F3/0601 ,  G06F2003/0692

摘要： An architecture and a method for the automated configuration of a storage enclosure are disclosed. The disclosed storage enclosure includes an internal communications link between the storage controllers of the storage enclosure. The storage enclosure is operable to determine if the storage enclosure is a terminal storage enclosure or if the expansion ports of the storage enclosure are externally cabled. If the storage enclosure is the terminal storage enclosure, the internal communications link is enabled, providing a communications pathway between the two controllers that is internal to the storage enclosure.

摘要翻译： 公开了用于存储外壳的自动配置的架构和方法。 所公开的存储机箱包括存储机柜的存储控制器之间的内部通信链路。 存储机箱可操作以确定存储机箱是终端存储机箱还是存储机柜的扩展端口是外部电缆连接。 如果存储机柜是终端存储机柜，则启用内部通信链路，从而在存储机柜内部的两个控制器之间提供通信路径。

公开(公告)号：US20050123956A1

公开(公告)日：2005-06-09

申请号：US10951983

申请日：2004-09-27

申请人： John Blume ,  Yanxiang Cao ,  Glenn McGall ,  Kyle Cole ,  Fredrick Christians ,  Kai Wu ,  Linda Hsie ,  Charles Miyada ,  Anthony Barone ,  Vivi Truong

发明人： John Blume ,  Yanxiang Cao ,  Glenn McGall ,  Kyle Cole ,  Fredrick Christians ,  Kai Wu ,  Linda Hsie ,  Charles Miyada ,  Anthony Barone ,  Vivi Truong

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q1/683 ,  C12Q2565/501 ,  C12Q2525/119 ,  C12Q2521/531

摘要： In one aspect of the invention, methods and compositions are provided for fragmenting nucleic acid samples. Fragmented nucleic acid samples may be used for hybridization with microarrays.

摘要翻译： 在本发明的一个方面，提供了用于片段化核酸样品的方法和组合物。 分离的核酸样品可用于与微阵列杂交。

公开(公告)号：US06613516B1

公开(公告)日：2003-09-02

申请号：US09689937

申请日：2000-10-11

申请人： Fred C. Christians ,  Duc Do ,  Thomas Gingeras ,  Kevin Gunderson ,  Charles G. Miyada ,  Carsten Rosenow ,  Kai Wu ,  Qing Yang

发明人： Fred C. Christians ,  Duc Do ,  Thomas Gingeras ,  Kevin Gunderson ,  Charles G. Miyada ,  Carsten Rosenow ,  Kai Wu ,  Qing Yang

IPC分类号： C12Q168

CPC分类号： C12Q1/6806 ,  C12N15/1006 ,  C12Q1/6813 ,  C12Q1/6837 ,  Y10S977/958 ,  C12Q2521/319 ,  C12Q2563/131

摘要： The presently claimed invention provides methods, compositions, and apparatus for studying nucleic acids. Specifically, the present invention provides a novel enrichment and labeling strategy for ribonucleic acids. In one embodiment, the invention provides enriching for a population of interest in a complex population by diminishing the presence of a target sequence. In a further embodiment, the invention can be used to reproducibly label and detect extremely small amounts of nucleic acids.

摘要翻译： 本发明提供了用于研究核酸的方法，组合物和装置。 具体地说，本发明提供了核糖核酸的新型富集和标记策略。 在一个实施方案中，本发明通过减少靶序列的存在来提供对复杂群体中的感兴趣群体的富集。 在另一个实施方案中，本发明可用于可重复地标记和检测极少量的核酸。

公开(公告)号：US6117977A

公开(公告)日：2000-09-12

申请号：US840062

申请日：1997-04-24

申请人： Laurence A. Lasky ,  Kai Wu

发明人： Laurence A. Lasky ,  Kai Wu

IPC分类号： C07K14/705 ,  C07K14/00 ,  C07H21/04 ,  C12N15/00 ,  C12N15/03

CPC分类号： C07K14/7056 ,  C07K14/70564 ,  C07K2319/00 ,  C07K2319/30

摘要： The invention concerns novel members of the endocytic type C lectin family and methods and means for producing them. The native polypeptides of the invention are characterized by containing a signal sequence, a cysteine rich domain, a fibronectin type II domain, 8 type C lectin domains, a transmembrane domain and a cytoplasmic domain. Nucleotide sequences encoding such polypeptides, vectors containing the nucleotide sequences, recombinant host cells transformed with the vectors, and methods for the recombinant production for the novel type C lectins are also within the scope of the invention.

摘要翻译： 本发明涉及内吞型C凝集素家族的新成员及其生产方法和方法。 本发明的天然多肽的特征在于含有信号序列，富含半胱氨酸的结构域，纤连蛋白II型结构域，8型凝集素结构域，跨膜结构域和胞质结构域。 编码这种多肽的核苷酸序列，含有核苷酸序列的载体，用载体转化的重组宿主细胞，以及用于新型C型凝集素重组生产的方法也在本发明的范围内。

公开(公告)号：US09783889B2

公开(公告)日：2017-10-10

申请号：US13430278

申请日：2012-03-26

申请人： Gwo-Chuan Tzu ,  Xiaoxiong Yuan ,  Amit Khandelwal ,  Avgerinos V. Gelatos ,  Olkan Cuvalci ,  Kai Wu ,  Michael P. Karazim

发明人： Gwo-Chuan Tzu ,  Xiaoxiong Yuan ,  Amit Khandelwal ,  Avgerinos V. Gelatos ,  Olkan Cuvalci ,  Kai Wu ,  Michael P. Karazim

IPC分类号： C23C16/458 ,  C23C16/46 ,  C23C16/455 ,  C23C16/48 ,  C23C16/52

CPC分类号： C23C16/46 ,  C23C16/45565 ,  C23C16/4583 ,  C23C16/4585 ,  C23C16/4586 ,  C23C16/481 ,  C23C16/52

摘要： In some embodiments, an apparatus for variable substrate temperature control may include a heater moveable along a central axis of a substrate support; a seal ring disposed about the heater, the seal ring configured to interface with a shadow ring disposed above the heater to form a seal; a plurality of spacer pins configured to support a substrate and disposed within a plurality of through holes formed in the heater, the plurality of spacer pins moveable parallel to the central axis, wherein the plurality of spacer pins control a first distance between the substrate and the heater and a second distance between the substrate and the shadow ring; and a resilient element disposed beneath the seal ring to bias the seal ring toward a backside surface of the heater.