公开(公告)号：US20090054636A1

公开(公告)日：2009-02-26

申请号：US12206859

申请日：2008-09-09

Applicant: Ghazala Hashmi ,  Michael Seul ,  Marion E. Reid ,  Michael Pierce

Inventor： Ghazala Hashmi ,  Michael Seul ,  Marion E. Reid ,  Michael Pierce

IPC: C07H21/04

CPC classification number: C12Q1/6881 ,  C12Q2600/156 ,  C12Q2600/16

Abstract: Disclosed are a method and an algorithm for genetic cross-matching based on the comparison of recipient and donor genotypes—and the underlying combinations of alleles and haplotypes. The method of the invention, rather than focusing on phenotype prediction, instead relies on a comparison of genetic variants identified in the recipient and available donors, whose information preferably will be compiled in a widely available donor registry, to maximize molecular compatibility. The genotypes can be matched based on the weighted clinical significance of a genotypic difference between donor and recipient, such that certain mismatches are more acceptable than others.

Abstract translation: 公开了基于接受者和供体基因型的比较以及等位基因和单体型的潜在组合的遗传交叉匹配的方法和算法。 本发明的方法而不是侧重于表型预测，而是依赖于在接受者和可用供体中鉴定的遗传变异体的比较，其信息优选地将在广泛可用的供体登记册中编译，以最大化分子相容性。 可以基于供体和受体之间的基因型差异的加权临床意义来匹配基因型，使得某些错配比其他错配更可接受。

公开(公告)号：US20080318211A9

公开(公告)日：2008-12-25

申请号：US10915153

申请日：2004-08-09

Applicant: Michael Seul ,  Richard Ebright

Inventor： Michael Seul ,  Richard Ebright

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/567

CPC classification number: C40B20/04 ,  B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/04 ,  C40B50/16 ,  C40B70/00 ,  G01N21/29 ,  G01N21/64 ,  G01N21/6458 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/6845 ,  G01N2021/1765

Abstract: Disclosed is a method for the physico-chemical encoding of a collection of beaded resin (“beads”) allowing determination of the chemical identity of bead-anchored compounds, following identification of beads bearing compounds of interest in an assay, by in-situ interrogation of individual beads, which does not require isolation of the beads of interest. These methods can be used to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing and other biochemical assays.

Abstract translation: 公开了一种用于物理化学编码珠粒树脂（“珠粒”）的集合的方法，其通过原位询问在鉴定在化验中带有感兴趣化合物的珠子之后，确定珠状锚定化合物的化学特性 的单个珠粒，其不需要分离感兴趣的珠粒。 这些方法可用于在应用中实现颜色编码策略，包括基于珠的组合化合物文库的超高通量筛选，以及多重诊断和环境测试以及其他生物化学测定。

公开(公告)号：US20080282483A1

公开(公告)日：2008-11-20

申请号：US11685875

申请日：2007-03-14

Applicant: Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

Inventor： Sukanta Banerjee ,  Cecilia Georgescu ,  Michael Seul

IPC: D06P3/79 ,  D06P3/04 ,  D06P3/00

CPC classification number: C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

Abstract: Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.

Abstract translation: 通过将微粒浸入包含溶解在三元溶剂系统中的选定溶质的浴中来获得溶质负载的聚合物微粒。 三元体系的第一溶剂是溶质和形成微粒的聚合物的强溶剂。 第二溶剂是用于溶质和聚合物（调谐溶剂）的弱溶剂或非溶剂。 第三溶剂是用于溶质和聚合物的弱溶剂或非溶剂，但是作为相对于第一和第二溶剂的共溶剂，因为其可与第一溶剂和第二溶剂混溶。 通过调节溶质相对于微粒聚合物的比例，调节三元溶剂体系的组成，主要是调谐溶剂的量来控制掺入微粒的溶质量。 该方法对于提供包含可区分的染料负载，特别是可区分的荧光染料负载的组合编码的微粒的文库是特别有用的。

公开(公告)号：US07335153B2

公开(公告)日：2008-02-26

申请号：US10192352

申请日：2002-07-09

Applicant: Michael Seul ,  Chiu Wo Chau ,  Hui Huang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Ye Hong

Inventor： Michael Seul ,  Chiu Wo Chau ,  Hui Huang ,  Sukanta Banerjee ,  Jiacheng Yang ,  Ye Hong

IPC: C40B20/02

CPC classification number: C12Q1/6837 ,  B01J19/0046 ,  B01J2219/00274 ,  B01J2219/00317 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00648 ,  B01J2219/00662 ,  G01N33/54306 ,  C12Q2563/149

Abstract: This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and/or multichip arrays of this invention are useful for chemical and biological assays.

Abstract translation: 本发明提供高单位密度的微粒阵列和组装这种阵列的方法。 阵列中的微粒可以用给定靶分析物特异性的化学或生物实体来官能化。 本发明的高单位密度阵列形成在芯片上，芯片可以根据本文所述的方法组合以形成多芯片阵列。 本发明的芯片和/或多芯片阵列可用于化学和生物测定。

公开(公告)号：US20070264641A1

公开(公告)日：2007-11-15

申请号：US11438723

申请日：2006-05-22

Applicant: Alice Li ,  Ghazala Hashmi ,  Michael Seul

Inventor： Alice Li ,  Ghazala Hashmi ,  Michael Seul

IPC: C12Q1/68

CPC classification number: C12Q1/6827

Abstract: The invention provides methods and processes for the identification of polymorphisms at one or more designated sites, without interference from non-designated sites located within proximity of such designated sites. Probes are provided capable of interrogation of such designated sites in order to determine the composition of each such designated site. By the methods of this invention, one or more mutations within the CFTR gene and the HLA gene complex can be can be identified.

Abstract translation: 本发明提供用于在一个或多个指定位点识别多态性的方法和过程，而不受位于该指定位点附近的非指定位点的干扰。 提供能够询问这些指定地点的探测器，以确定每个这样的指定地点的组成。 通过本发明的方法，可以鉴定CFTR基因和HLA基因复合体内的一个或多个突变。

公开(公告)号：US07262063B2

公开(公告)日：2007-08-28

申请号：US10034727

申请日：2001-12-26

Applicant: Sukanta Banerjee ,  Kairali Podual ,  Michael Seul

Inventor： Sukanta Banerjee ,  Kairali Podual ,  Michael Seul

IPC: G01N33/551

CPC classification number: C08J3/075 ,  C08J3/28 ,  C08J2333/26 ,  C08J2425/06 ,  C12Q1/6837 ,  G01N33/5005 ,  G01N33/545 ,  G03F7/0047 ,  G03F7/164 ,  Y10T428/249952 ,  Y10T428/25

Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)—microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation of said assemblies into other structures. The present invention. also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, micro-reactors, smart materials. Miniaturized format for generation of multifunctional thin films. Provides a simple set-up to synthesize thin films of tailored composition and morphology:

Abstract translation: 本发明涉及一种用于产生功能组织的空间图案化聚合物 - 聚合物 - 微粒复合材料的系统方法，其包括图案化的自支撑有机（聚合物）膜和有机（聚合） - 微粒复合膜的交流电场介导组装 定制组合和形态; 本发明还涉及将所述组件结合到其它结构中。 本发明。 还涉及这种功能组件在材料科学和生物学中的应用。 其他应用领域包括传感器，催化剂，膜，微反应器，智能材料。 用于生成多功能薄膜的小型化格式。 提供简单的设置来合成定制组合和形态的薄膜：

公开(公告)号：US20070093968A1

公开(公告)日：2007-04-26

申请号：US11585068

申请日：2006-10-23

Applicant: Yi Zhang ,  Michael Seul

Inventor： Yi Zhang ,  Michael Seul

IPC: G01N33/567 ,  G06F19/00

CPC classification number: G06F19/3456 ,  G06F19/3481 ,  G16B20/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  G16H10/60 ,  G16H50/70

Abstract: Disclosed are methods for establishing the compatibility between two blood types on the basis of cross-matching (under a designated rule of stringency) the minor blood group genotypes of recipient and prospective donors. To determine compatibility, the blood group genotypes are mapped to corresponding phenotypes according to the expression states associated with a set of underlying haplotypes, and compatibility is established by establishing the compatibility of blood types constructed as a combination of constituent phenotypes. The bit strings are matched, preferably using an algorithm expression. Where ambiguity in mapping genotypes to haplotypes exists, it can be reduced based on frequency of occurrence of the haplotypes in the sample population, or resolved by gametic phasing. Such reduction or resolution of ambiguity is particularly desirable where mismatches in the antigens expressed by the constituent haplotypes have greater clinical significance.

Abstract translation: 公开的是基于接受者和潜在供体的次要血型基因型的交叉匹配（在指定的严格规则）的基础上建立两种血型之间的相容性的方法。 为了确定相容性，根据与一组潜在单倍型相关联的表达状态，将血型基因型映射到相应的表型，并且通过建立构成为构成表型的组合的血型相容性建立相容性。 比特串匹配，最好使用算法表达式。 如果存在将基因型映射到单倍型的歧义，则可以根据样本群体中单倍型的发生频率来减少，或者通过配子定相来解决。 在组成单元型表达的抗原中的错配具有更大的临床意义的情况下，这种模糊的减少或解决是特别理想的。

公开(公告)号：US07202038B2

公开(公告)日：2007-04-10

申请号：US10913987

申请日：2004-08-06

Applicant: Michael Seul

Inventor： Michael Seul

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2525/186 ,  C12Q2533/101 ,  C12Q2535/101 ,  C12Q2565/102

Abstract: Disclosed is a method of analyzing tandem repeats using one or more probes, each such probe may lack an anchoring sequence but contains one or more tandem repeat sequences complementary to the target tandem repeat sequences. In one embodiment, each probe is attached, via its 5′ end, to an encoded microparticle (“bead”), wherein the code—implemented by way of a color scheme—identifies the sequence and length of the probe attached thereto. Also disclosed are methods relating to the analysis of partial duplex configurations involving only partial overlap between probe and target repeats and thus “overhangs” of probe repeats on the 3′ and/or 5′ ends of the target repeats.

Abstract translation: 公开了使用一个或多个探针分析串联重复序列的方法，每个这样的探针可能缺少锚定序列，但是含有与靶序列重复序列互补的一个或多个串联重复序列。 在一个实施方案中，每个探针通过其5'末端连接到编码的微粒（“珠粒”），其中通过配色方案代码实现 - 识别附着于其上的探针的序列和长度。 还公开了与仅涉及探针和目标重复之间部分重叠的部分双相结构分析相关的方法，因此在目标重复的3'和/或5'末端上的探针重复“突出”。

公开(公告)号：US07156315B2

公开(公告)日：2007-01-02

申请号：US10365993

申请日：2003-02-13

Applicant: Michael Seul ,  Chiu Wo Chau

Inventor： Michael Seul ,  Chiu Wo Chau

IPC: G06K19/06

CPC classification number: H05H3/04 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00572 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00713 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0652 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0454 ,  B01L2400/086 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B40/00 ,  C40B60/10 ,  G01N15/0266 ,  G01N30/0005 ,  G01N33/54313 ,  G01N2015/0288 ,  G01N2015/149 ,  G01N2030/0035 ,  G02B3/0012 ,  G02B3/0056 ,  Y10T436/25125 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particulates and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components. The invention is also for a system and method for programmable illumination pattern generation, including a novel method and apparatus to generate patterns of illumination and project them onto planar surfaces or onto planar interfaces such as the interface formed by an electrolyte-insulator-semiconductor (EIS), e.g., as described herein. This enables the creation of patterns or sequences of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest. The use of the LCD technology provides flexibility and control over spatial layout, temporal sequences and intensities (“gray scales”) of illumination patterns. The latter capability permits the creation of patterns with abruptly changing light intensities or patterns with gradually changing intensity profiles.

公开(公告)号：US20060228742A1

公开(公告)日：2006-10-12

申请号：US11439694

申请日：2006-05-23

Applicant: Ghazala Hashmi ,  Michael Seul

Inventor： Ghazala Hashmi ,  Michael Seul

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2600/156 ,  C12Q2600/16 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/543 ,  C12Q2563/107 ,  C12Q2565/501

Abstract: Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is complementary to a corresponding wild type allele), with probes bound to encoded particles (e.g., beads) wherein the encoding allows identification of the attached probe. The methods relate to avoiding cross-hybridization by selection of probes and amplicons, as well as separation of reactions of certain probes and amplicons where a homology threshold is exceeded. Methods of correcting a fluorescent image using a background map, where the particles also contain an optical encoding system, are also disclosed.

Abstract translation: 描述了测定设计和测定图像校正的方法，其可用于使用探针对阵列（在一个方面中，其中一个成员与特定突变体互补的突变和多态性）的多重遗传筛选（包括CF相关突变体和多态性） 多态等位基因和另一个成员与相应的野生型等位基因互补），其中探针与编码的颗粒结合（例如，珠粒），其中编码允许鉴定附着的探针。 该方法涉及通过选择探针和扩增子来避免交叉杂交，以及分离某些探针和扩增子的反应，其中超过同源性阈值。 还公开了使用背景图校正荧光图像的方法，其中颗粒还包含光编码系统。