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128 条记录 (耗时 0.064 秒)

    CELLOMICS SYSTEM
    71.
    发明申请
    CELLOMICS SYSTEM 审中-公开

    公开(公告)号：US20090325215A1

    公开(公告)日：2009-12-31

    申请号：US12472037

    申请日：2009-05-26

    申请人： Kazunori Okano ,  Kenji Yasuda

    发明人： Kazunori Okano ,  Kenji Yasuda

    IPC分类号： C12Q1/02 ,  C12M1/00

    CPC分类号： B01L7/50 ,  A01N1/0284 ,  G01N33/5061 ,  G01N33/566

    摘要： In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

    Method of inspecting a DNA chip and apparatus thereof
    73.
    发明申请
    Method of inspecting a DNA chip and apparatus thereof 有权
    检查DNA芯片的方法及其装置

    公开(公告)号：US20070154938A1

    公开(公告)日：2007-07-05

    申请号：US11709120

    申请日：2007-02-22

    申请人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    发明人： Yoshitada Oshida ,  Toshihiko Nakata ,  Tomoaki Sakata ,  Kenji Yasuda ,  Satoshi Takahashi

    IPC分类号： C12Q1/68 ,  G01J3/42

    CPC分类号： G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  Y10S436/80 ,  Y10T436/143333

    摘要： A DNA inspecting apparatus including driving means for relatively changing positions of the multi-spot lights and a position of the DNA chip so as to detect the fluorescent lights in such a manner that a desired area on the DNA chip is irradiated with the multi-spot lights, and a control system for determining and inspecting DNA information about the to-be-inspected DNA chip from fluorescent light intensities and fluorescent light positions of the desired area on the DNA chip, the fluorescent light intensities and the fluorescent light positions being detected by the driving means and the fluorescent light detecting means.

    摘要翻译： 一种DNA检查装置，包括用于相对改变多点灯位置的驱动装置和DNA芯片的位置，以便以这样的方式检测荧光，使得DNA芯片上的期望区域被多点照射 灯和用于从DNA芯片上的期望区域的荧光强度和荧光灯位置确定和检查关于待检查DNA芯片的DNA信息的控制系统，荧光强度和荧光灯位置由 驱动装置和荧光检测装置。

    Apparatus for microscopic observation of long-term culture of single cell
    74.
    发明授权
    Apparatus for microscopic observation of long-term culture of single cell 有权
    用于显微镜观察单细胞长期培养的装置

    公开(公告)号：US07092154B2

    公开(公告)日：2006-08-15

    申请号：US10432396

    申请日：2001-11-22

    申请人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

    发明人： Kenji Yasuda ,  Kunihiko Kaneko ,  Tetsuya Yomo ,  Ippei Inoue ,  Yuichi Wakamoto

    IPC分类号： G01N21/01 ,  G02B21/34

    CPC分类号： C12M25/02 ,  B01L3/502 ,  C12M25/00 ,  C12M41/36 ,  C12M41/46 ,  G01N35/028 ,  G01N35/1002 ,  G02B21/34

    摘要： An apparatus is provided with a cell culture container having a cell culture region made of a hole formed on a substrate, a semi-permeable membrane covering a top plane of the cell culture region and a culture medium replacement part provided over the semi-permeable membrane, a mechanism for supplying a cell culture medium into the cell culture container, and a microscopic optical mechanism for enabling long-term observation of the cell within the cell culture region. This apparatus makes it possible to culture a cell group originating from a particular single cell, to perform culture and observation while identifying cells to be subjected to interaction during the process of culturing cells, and observe a difference in variation between the particular cell and other cells. There is also provided a mechanism which makes it possible to collect only a cell assuming a particular state and perform analysis or biochemical measurement of a gene of the cell, an expressed mRNA and the like.

    摘要翻译： 本发明提供一种细胞培养容器，该细胞培养容器具有由形成在基材上的孔形成的细胞培养区域，覆盖细胞培养区域的顶面的半透膜和设置在半透膜上的培养基置换部件 用于向细胞培养容器供给细胞培养基的机理，以及能够长期观察细胞培养区域内的细胞的微观光学机构。 该装置可以培养来自特定单细胞的细胞群，进行培养和观察，同时确定在培养细胞过程中待相互作用的细胞，并观察特定细胞与其它细胞之间的差异 。 还提供了一种使得可以仅收集假设特定状态的细胞并进行细胞基因，表达的mRNA等的分析或生物化学测量的机制。

    Droplet operation device
    75.
    发明申请
    Droplet operation device 失效
    液滴操作装置

    公开(公告)号：US20060039828A1

    公开(公告)日：2006-02-23

    申请号：US10525521

    申请日：2003-08-26

    申请人： Kenji Yasuda ,  Takanori Ichiki ,  Kazunori Okano

    发明人： Kenji Yasuda ,  Takanori Ichiki ,  Kazunori Okano

    IPC分类号： G01N21/00

    CPC分类号： B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N15/1468 ,  G01N35/00029 ,  G01N2015/149 ,  G01N2035/0097 ,  Y10T436/2575

    摘要： A droplet operation device having a substrate having a light transmissibility and subjected to a water repellent treatment, a means for supplying droplets onto the substrate, a means for transporting the droplets on the water repellent substrate, and a means for measuring the state of the droplets, comprising the light transmittable substrate (11) receiving the water repellent treatment on the surface thereof, solvent feeding ports (21), (22), and (23) for leading reagent or specimen droplets (51) onto the substrate (11), optical measuring units (61), (62), and (63), and electric field application units (71), (72), and (73), whereby the inside of the droplets can be observed with a microscope, and the results of the reaction of the droplets against the other droplets can be measured and classified by stopping and moving the droplets in any direction.

    摘要翻译： 一种液滴操作装置，具有具有透光性且进行防水处理的基板，用于将液滴供给到基板上的装置，用于在憎水基板上输送液滴的装置，以及用于测量液滴状态的装置 包括在其表面上接受防水处理的透光性基材（11），将试剂或试样液滴（51）引导到基材（11）上的溶剂供给口（21），（22）和（23） 光学测量单元（61），（62）和（63）以及电场施加单元（71），（72）和（73），由此可以用显微镜观察液滴的内部，结果 可以通过在任何方向上停止和移动液滴来测量和分类液滴对其他液滴的反应。

    Nucleic acid analysis chip and nucleic acid analyzer
    76.
    发明申请
    Nucleic acid analysis chip and nucleic acid analyzer 审中-公开
    核酸分析芯片和核酸分析仪

    公开(公告)号：US20050272039A1

    公开(公告)日：2005-12-08

    申请号：US10525876

    申请日：2003-08-26

    申请人： Kenji Yasuda

    发明人： Kenji Yasuda

    IPC分类号： G01N21/64 ,  C07H21/00 ,  C12M1/00 ,  G01N21/03 ,  G01N21/17 ,  G01N33/483 ,  G01N33/53 ,  C12Q1/68 ,  C12M1/34

    CPC分类号： C07H21/00

    摘要： Means for performing direct quantitative analysis of the components of nucleic acids of cell 103 placed in a well as culture container (102) of a cell culture microchamber, comprising means for optically measuring each of the culture containers (102) of cell culture microchamber, means for effecting optical heating, means for introducing a reaction fluid in each of the culture containers (102) of cell culture microchamber and means for inhibiting diffusion and evaporation of the reaction fluid in each of the culture containers (102). These enable analyzing the components of nucleic acids of each of the cells cultured in single-cell culture microchamber.

    摘要翻译： 用于对放置在细胞培养物微室的培养容器（102）的孔中的细胞103的核酸成分进行直接定量分析的方法，包括用于光学测量细胞培养物微室的培养容器（102）的装置， 用于实现光学加热的装置，用于在细胞培养微量室的每个培养容器（102）中引入反应流体的装置和用于抑制每个培养容器（102）中的反应流体的扩散和蒸发的装置。 这些能够分析在单细胞培养微量室中培养的每种细胞的核酸成分。

    Method and system for offering gene analysis information and autentication identification method
    77.
    发明申请
    Method and system for offering gene analysis information and autentication identification method 审中-公开
    提供基因分析信息和自动识别方法的方法和系统

    公开(公告)号：US20050069874A1

    公开(公告)日：2005-03-31

    申请号：US10432932

    申请日：2000-11-30

    申请人： Kenji Yasuda ,  Yuichi Takegawa

    发明人： Kenji Yasuda ,  Yuichi Takegawa

    IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00 ,  G06F19/28 ,  G06F17/60

    CPC分类号： G06Q50/24 ,  G16B50/00 ,  G16H10/40

    摘要： A service of analyzing a homo gene is offered in the form conforming to the requirement of the customer. A service of carrying out gene analysis required by the customer through an electronic request method. A gene information access center is set up, where the gene and its analysis information of the customer is stored. The gene is analyzed in response to the request of the customer and the result is provided. Information meeting the needs of the customer from among a huge amount of information on genome genes is provided with time and in phase.

    摘要翻译： 以符合客户要求的形式提供分析同源基因的服务。 通过电子请求方式进行客户要求的基因分析服务。 建立基因信息访问中心，存储客户的基因及其分析信息。 根据客户的要求分析基因，并提供结果。 提供了从大量基因组基因信息中满足客户需求的信息，并且提供了时间和相位。

    Apparatus for the recovery of nucleic acids
    79.
    发明授权
    Apparatus for the recovery of nucleic acids 失效
    用于回收核酸的装置

    公开(公告)号：US06492162B1

    公开(公告)日：2002-12-10

    申请号：US09457290

    申请日：1999-12-09

    申请人： Toshinari Sakurai ,  Shinichi Fukuzono ,  Kenji Yasuda

    发明人： Toshinari Sakurai ,  Shinichi Fukuzono ,  Kenji Yasuda

    IPC分类号： C12M100

    CPC分类号： C12N15/1006

    摘要： Methods and apparatus are provided for the recovery of nucleic acids from a nucleic acid-containing material by which nucleic acids can be rapidly and easily recovered at a high purity without deteriorating the yield. The methods are composed of step 1 for promoting the release of nucleic acids from a nucleic acid-containing material, step 2 for mixing the released nucleic acids with an accelerator substance for the binding of nucleic acids to a solid phase, step 3 for bringing the mixture in contact with a solid phase bondable to nucleic acids, step 4 for isolating the solid phase from a liquid, step 5 for washing the solid phase with a solution containing a salt, and a step 6 for eluting the nucleic acids from the solid phase. Accordingly, nucleic acids at a suitable purity for genetic tests or gene analyses can be rapidly and easily recovered without the use of hazardous substances. The apparatus may contain a reactor, at least one pipette, a purified product container, transport mechanisms for the reactor and purified product container, motors to control the pipette and transport mechanisms, a mechanical control unit to control the motors, and a computer that controls the mechanical control unit.

    摘要翻译： 提供了用于从含核酸材料回收核酸的方法和装置，通过该方法和装置可以以高纯度快速且容易地回收核酸而不降低产率。 该方法由用于促进核酸从含核酸材料释放的步骤1组成，步骤2用于将释放的核酸与用于将核酸与固相结合的促进剂物质混合，步骤3 与可与核酸结合的固相接触的混合物，用于从液体中分离固相的步骤4，用含有盐的溶液洗涤固相的步骤5，和从固相中洗脱核酸的步骤6 。 因此，用于遗传测试或基因分析的合适纯度的核酸可以快速且容易地回收而不使用有害物质。 该装置可以包含反应器，至少一个移液管，净化产品容器，用于反应器和净化产品容器的输送机构，用于控制移液管和输送机构的马达，用于控制马达的机械控制单元和控制马达的计算机 机械控制单元。