公开(公告)号：US09784711B2

公开(公告)日：2017-10-10

申请号：US14402717

申请日：2014-01-06

申请人： TECAN TRADING AG

发明人： Daniel Hoffmeyer ,  Tiffany Lay ,  Travis Lee ,  Marc N. Feiglin ,  Werner Halg ,  Anne R. Kopf-Sill

IPC分类号： G01N27/447 ,  B01L3/00 ,  B81B1/00

CPC分类号： G01N27/44791 ,  B01L3/502715 ,  B01L3/502792 ,  B01L3/505 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2300/044 ,  B01L2300/123 ,  B01L2400/0427 ,  B81B1/00 ,  G01N27/44756

摘要： A disposable cartridge used in a digital microfluidics system has a bottom layer with first hydrophobic surface, a rigid cover plate with second hydrophobic surface, and a gap there-between. The bottom layer is a flexible film on an uppermost surface of a cartridge accommodation site of a system, attracted to and spread over the uppermost surface by an underpressure. A lower surface of the plate and the flexible bottom layer are sealed to each other. The assembled cartridge is removed from the cartridge accommodation site in one piece and potentially includes samples and processing fluids. The system has a base unit and a cartridge accommodation site with an electrode array of individual electrodes and a central control unit for controlling selection of individual electrodes and for providing these electrodes with individual voltage pulses for manipulating liquid droplets within the gap by electrowetting.

公开(公告)号：US20170284961A1

公开(公告)日：2017-10-05

申请号：US15508069

申请日：2015-09-04

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  UNIVERSITE PARIS-SUD

发明人： Anne-Marie HAGHIRI-GOSNET ,  Antoine PALLANDRE ,  Jean GAMBY ,  Daniel ROSE ,  Sebastien MEANCE

IPC分类号： G01N27/447 ,  B01L3/00

CPC分类号： G01N27/44752 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/143 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/16 ,  B01L2400/0418 ,  B01L2400/0421 ,  G01N27/44791 ,  G01N2030/285

摘要： The present invention relates to a microfluidic analysis device (1) including: a substrate (20) wherein a separation channel (10) is arranged, in which an electrolyte flows, a portion of the separation channel (10) being covered with a polarisable surface (11); two longitudinal field electrodes (8a, 8b) arranged on either side of the separation channel (10); at least one control electrode (6a, 6b) positioned in the separation channel (10), the control electrode (6a, 6b) being suitable for polarising the polarisable surface (11) so as to control the speed of the electro-osmotic flow in the separation channel (10); the microfluidic analysis device (1) being characterised in that the polarisable surface (11) includes an insulating sub-layer (12) made of amorphous silicon carbide (SiC) and an upper polarisable layer (13) in direct contact with the electrolyte, the control electrodes (6a, 6b) being positioned between the insulating sub-layer (12) and the upper polarisable layer (13).

公开(公告)号：US09772323B2

公开(公告)日：2017-09-26

申请号：US15439497

申请日：2017-02-22

申请人： Pacific Biosciences of California, Inc.

发明人： Stephen Turner ,  Benjamin Flusberg

IPC分类号： C12Q1/68 ,  G01N27/447 ,  G01N33/487 ,  B82Y5/00 ,  B82Y15/00

CPC分类号： G01N33/48721 ,  B82Y5/00 ,  B82Y15/00 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2565/631 ,  G01N27/447 ,  G01N27/44791 ,  C12Q2563/116 ,  C12Q2527/113

摘要： The invention relates to devices and methods for nanopore sequencing. The invention provides for using the signals from N monomeric units (n-mers) within the nanopore to provide sequence information. The invention includes substrates having arrays of nanopores with incorporated electronic circuits, for example, in semiconductor substrates. In some cases, the arrays of nanopores reside in discrete reservoirs.

公开(公告)号：US09766206B2

公开(公告)日：2017-09-19

申请号：US14039995

申请日：2013-09-27

申请人： ProteinSimple

发明人： Tom W. Yang ,  David John Roach

IPC分类号： G01N27/447 ,  C07K1/28 ,  C07K1/26

CPC分类号： G01N27/44782 ,  C07K1/26 ,  C07K1/285 ,  G01N27/44704 ,  G01N27/44726 ,  G01N27/44791

摘要： An apparatus includes a body portion that defines a reservoir and a set of substantially flexible capillaries. The set of substantially flexible capillaries are fixedly coupled to the body portion and in fluid communication with the reservoir. A connector is configured to be coupled to the body portion to be in fluid communication with the reservoir and the set of substantially flexible capillaries. The connector is further configured to be coupled to a vacuum source. The apparatus is arranged such that at least a part of the body portion is electrically conductive. Methods for separating and detecting an analyte from a biological sample with the apparatus are also provided. For example, methods for separating and detecting one or more proteins from a cellular lysate or a purified protein are also provided.

公开(公告)号：US09765326B2

公开(公告)日：2017-09-19

申请号：US14676626

申请日：2015-04-01

申请人： STMICROELECTRONICS S.R.L.

发明人： Francesco Ferrara ,  Marco Angelo Bianchessi

IPC分类号： G01N27/447 ,  C12N15/10 ,  B01L3/00 ,  G01N33/487 ,  C12Q1/68

CPC分类号： C12N15/101 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0627 ,  C12Q1/6869 ,  C12Q2523/307 ,  C12Q2563/157 ,  C12Q2565/607 ,  G01N27/44791 ,  G01N33/48721

摘要： An apparatus for nucleic acid sequencing includes a nanochannel and a conveying device, configured to move a nucleic acid strand through the nanochannel. The conveying device includes: a first electrode, a second electrode, and a third electrode, which are arranged along the nanochannel so as to be in contact with a fluid occupying the nanochannel, the second electrode being arranged between the first electrode and the third electrode; and a control unit configured to apply a first voltage, a second voltage, and a third voltage, respectively, to the first electrode, the second electrode and the third electrode, for controlling movement of the nucleic acid strand through the nanochannel.

公开(公告)号：US09753006B2

公开(公告)日：2017-09-05

申请号：US14743637

申请日：2015-06-18

申请人： INTERNATIONAL BUSINESS MACHINES CORPORATION

发明人： Qing Cao ,  Kangguo Cheng ,  Zhengwen Li ,  Fei Liu ,  Zhen Zhang

IPC分类号： G01N27/414

CPC分类号： G01N27/44791 ,  G01N27/4146 ,  G01N33/48707 ,  H01L21/02236 ,  H01L21/3065 ,  H01L21/31116

摘要： A sensor includes a semiconductor substrate having first pointed nodes extending into a channel from a first side of the channel. Second pointed nodes extend into the channel from a second side of the channel, which is opposite the first side. The second pointed nodes being self-aligned to the first pointed nodes on the opposite side of the channel. The first pointed nodes and the second pointed nodes are connected to a circuit to detect particles in the channel.

公开(公告)号：US09751083B2

公开(公告)日：2017-09-05

申请号：US14680671

申请日：2015-04-07

申请人： University of Macau

发明人： Jie Gao ,  Tianlan Chen ,  Cheng Dong ,  Yanwei Jia ,  Pui-In Mak ,  Mang-I Vai ,  Rui Paulo da Silva Martins

IPC分类号： G01N27/27 ,  B01L3/00 ,  G01N27/447

CPC分类号： B01L3/502792 ,  B01L2200/143 ,  B01L2300/025 ,  B01L2300/0887 ,  B01L2400/0427 ,  G01N27/44704 ,  G01N27/44756 ,  G01N27/44791

摘要： According to one aspect of the present disclosure, a digital microfluidic system is provided. The digital microfluidic system includes a device, a control electronics, a field programmed gate array (FPGA), and a computer. The device includes a droplet on an electrode array, where the electrode array includes a plurality of electrodes. The control electronics connects to the device and provides an actuation pulse to the electrodes, where the control electronics generates a capacitance-derived frequency signal. The FPGA connects to the control electronics and collects the capacitance-derived frequency signal. The computer connects to the FPGA, the computer uses a frequency of the capacitance-derived frequency signal to calculate a precise droplet position and generates a duration voltage signal.

公开(公告)号：US20170218443A1

公开(公告)日：2017-08-03

申请号：US15488311

申请日：2017-04-14

申请人： University of Washington ,  The UAB Research Foundation

发明人： Jens H. Gundlach ,  Michael Niederweis ,  Thomas Z. Butler ,  Mikhail Pavlenok ,  Mark A. Troll ,  Suja Sukumaran

IPC分类号： C12Q1/68 ,  G01N33/487 ,  G01N27/447

CPC分类号： C12Q1/6869 ,  A61K39/00 ,  A61K39/04 ,  C07K14/35 ,  C12N13/00 ,  G01N24/00 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721 ,  G01N33/6872 ,  C12Q2523/31 ,  C12Q2565/631

摘要： Provided herein are Mycobacterium smegmatis porin nanopores, systems that comprise these nanopores, and methods of using and making these nanopores. Such nanopores may be wild-type MspA porins, mutant MspA porins, wild-type MspA paralog porins, wild-type MspA homolog porins, mutant MspA paralog porins, mutant MspA homolog porins, or single-chain Msp porins. Also provided are bacterial strains capable of inducible Msp porin expression.

公开(公告)号：US09719962B2

公开(公告)日：2017-08-01

申请号：US14115976

申请日：2012-05-04

申请人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

发明人： Dimitrios Sideris ,  Alex Iles ,  Richard Jackson

IPC分类号： G01N27/447 ,  B01D57/02

CPC分类号： G01N27/44791 ,  B01D57/02 ,  G01N27/447 ,  G01N27/44713 ,  G01N27/44756 ,  Y10T29/49002

摘要： A device comprises an electric field applying assembly adapted to generate an electric field having a discrete electric field profile; a conducting volume and an electrical interface region provided between the conducting volume and the electric field applying assembly such that the discrete electric field is applied to the material by the electric field applying assembly at a location spaced from the conducting volume, wherein the electrical interface region comprises at least an ionically conductive material arranged adjacent to an in contact with the conducting volume; such that the discrete electric field applied by the electric field applying assembly is smoothed by the electrical interface region so that the electric field profile established within the conducting volume is substantially continuous.

公开(公告)号：US20170197213A1

公开(公告)日：2017-07-13

申请号：US15342914

申请日：2016-11-03

申请人： IntegenX Inc.

发明人： William D. Nielsen ,  Richard J. Belcinski ,  Gregory Bogdan ,  David Eberhart ,  Omar EL-Sissi ,  Stevan B. Jovanovich ,  Michael Recknor ,  Ezra Van Gelder ,  David W. Wyrick

IPC分类号： B01L3/00 ,  G01N27/447 ,  B01L7/00

CPC分类号： B01L3/502715 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N27/44743 ,  G01N27/44791 ,  G01N2035/00158 ,  G01N2035/0091

摘要： This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.