公开(公告)号：US20130266204A1

公开(公告)日：2013-10-10

申请号：US13755754

申请日：2013-01-31

Applicant: Fluidigm Corporation

Inventor： Emerson Chueng Quan ,  Colin Jon Taylor ,  Michael Lee ,  Christopher Ceasar ,  Greg Harris ,  Gang Sun

IPC: B01L3/00

CPC classification number: G01N33/00 ,  B01L3/5025 ,  B01L3/502707 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/0887 ,  B01L2400/0481 ,  B01L2400/0655 ,  Y10T436/11

Abstract: A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.

公开(公告)号：US20020183996A1

公开(公告)日：2002-12-05

申请号：US09894862

申请日：2001-06-27

Applicant: Fluidigm Corporation

Inventor： Michael Lee ,  Gajus Worthington

IPC: G06F017/50

CPC classification number: G06F17/50 ,  B01F13/0059 ,  B01F15/00824 ,  B01F15/00935 ,  B01J2219/00997 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/12 ,  B81B1/00 ,  B81C99/006 ,  G06F17/509 ,  G06F2217/16 ,  H01H2029/008

Abstract: The present invention generally relates to design automation techniques and more particularly to the design of customized microfluidic systems using a microfluidic computer aided design system. In one embodiment of the present invention the system includes a synthesis module for synthesizing software of a design into a component level description of the design. The design has a plurality of microfluidic components, and the component level description has symbols associated with the plurality of microfluidic components. The system further includes a design capture module, including a schematic entry tool, for placing and connecting the symbols on a schematic according to the design; and a functional analysis module for functionally simulating selected symbols of the schematic.

Abstract translation: 本发明一般涉及设计自动化技术，更具体地涉及使用微流控计算机辅助设计系统的定制微流体系统的设计。 在本发明的一个实施例中，系统包括用于将设计的软件合成到设计的组件级描述中的合成模块。 该设计具有多个微流体部件，并且部件级别描述具有与多个微流体部件相关联的符号。 该系统还包括设计捕获模块，其包括用于根据设计在符号放置和连接符号的原理图输入工具; 以及用于功能模拟原理图的所选符号的功能分析模块。

公开(公告)号：USD1038429S1

公开(公告)日：2024-08-06

申请号：US29811641

申请日：2021-10-15

Applicant: Fluidigm Corporation

Designer： Michael Makay ,  Benjamin Martin ,  Cole Derby ,  Kyle Wisdom Hukari ,  Michael Charles Norris

Abstract: FIG. 1 is a perspective view of a scientific instrument, showing our new design.
FIG. 2 is a front view of the instrument of FIG. 1.
FIG. 3 is a back view of the instrument of FIG. 1.
FIG. 4 is a left side view of the instrument of FIG. 1.
FIG. 5 is a right side view of the instrument of FIG. 1.
FIG. 6 is a top view of the instrument of FIG. 1; and,
FIG. 7 is a bottom view of the instrument of FIG. 1.
The broken lines in the drawings depict portions of the scientific instrument that form no part of the claimed design.

公开(公告)号：US12018323B2

公开(公告)日：2024-06-25

申请号：US17480067

申请日：2021-09-20

Applicant: Fluidigm Corporation

Inventor： Megan Anderson ,  Peilin Chen ,  Brian Fowler ,  Robert C. Jones ,  Fiona Kaper ,  Ronald Lebofsky ,  Andrew May

IPC: C12Q1/68 ,  C12N15/10 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/6855 ,  C12Q1/686 ,  C12Q1/6874

CPC classification number: C12Q1/6855 ,  C12N15/10 ,  C12N15/1065 ,  C12N15/65 ,  C12N15/66 ,  C12Q1/686 ,  C12Q1/6874

Abstract: Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

公开(公告)号：US11795494B2

公开(公告)日：2023-10-24

申请号：US17166992

申请日：2021-02-03

Applicant: Fluidigm Corporation

Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

IPC: C12Q1/6806 ,  C12Q1/686 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6806 ,  B01L3/502738 ,  C12Q1/686 ,  B01L3/50273 ,  B01L7/52 ,  B01L2300/087 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629 ,  C12Q1/6806 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

公开(公告)号：US10745748B2

公开(公告)日：2020-08-18

申请号：US16134855

申请日：2018-09-18

Applicant: Fluidigm Corporation

Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

IPC: G01N21/00 ,  C12Q1/686 ,  G01N21/64 ,  G01N27/447 ,  G02B21/16 ,  G01N21/84 ,  G01N21/75 ,  G02B21/36 ,  B01L3/00 ,  B01L7/00

Abstract: In some embodiments, a variety of devices and methods for conducting microfluidic analyses are utilized herein, including devices that can be utilized to conduct thermal cycling reactions such as nucleic acid amplification reactions. The devices include elastomeric components; in some instances, much or all of the device is composed of elastomeric material. Amplification products (amplicons) can be detected and distinguished (whether isolated in a reaction chamber or at any subsequent time) using routine methods for detecting nucleic acids. An example of a detection method includes hybridization to arrays of immobilized oligo or polynucleotides.

公开(公告)号：US10436698B2

公开(公告)日：2019-10-08

申请号：US16230570

申请日：2018-12-21

Applicant: Fluidigm Corporation

Inventor： Dmitry R. Bandura ,  Vladimir I. Baranov ,  Scott D. Tanner

IPC: G01N15/14 ,  H01J49/00 ,  H01J49/04 ,  G01N15/00

Abstract: An analytical instrument has a sample introduction system for generating a stream of particles from a sample. An ionization system atomizes and ionizes particles in the stream as they are received. The instrument has an ion pretreatment system and a mass analyzer. The ion pretreatment system is adapted to transport ions generated by the ionization system to the mass analyzer. The mass analyzer is adapted measure the amount of at least one element in individual particles from the stream by performing mass analysis on the ions from the atomized particles. The instrument can be adapted to measure the amount of many different tags, for example at least five different tags, at the same time to facilitate multi-parametric analysis of cells and other particles.

公开(公告)号：US20190292581A1

公开(公告)日：2019-09-26

申请号：US16373507

申请日：2019-04-02

Applicant: Fluidigm Corporation

Inventor： Camila Egidio ,  Ramesh Ramakrishnan ,  David Ruff

IPC: C12Q1/6804 ,  C12Q1/6848 ,  C12Q1/6851

Abstract: Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays.

公开(公告)号：US20190249238A1

公开(公告)日：2019-08-15

申请号：US16229786

申请日：2018-12-21

Applicant: Fluidigm Corporation

Inventor： Jason A.A. West ,  Brian Fowler ,  Tze-Howe Charn ,  Christian F. Johnson ,  Marc A. Unger

IPC: C12Q1/6855 ,  C12N15/10

CPC classification number: C12Q1/6855 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q2565/501 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2537/162 ,  C12Q2565/543 ,  C12Q2535/122

Abstract: This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the recognition-oligonucleotide-target polynucleotide hybrid is cleaved; and an adapter nucleic acid is ligated to the cleaved target polynucleotide, thereby forming a tagged nucleic acid sequence. Also provided is a method of forming a tagged single stranded cDNA; a method of forming a plurality of tagged heterogeneous nucleic acid sequences; a library of recognition-oligonucleotides; and methods for amplifying a cDNA sequence immobilized on a solid support. These methods and products can be used alone or in combination for integrated single cell sequencing, and can be adapted for use in a microfluidic apparatus or device.

公开(公告)号：US20190217302A1

公开(公告)日：2019-07-18

申请号：US16245537

申请日：2019-01-11

Applicant: Fluidigm Corporation

Inventor： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

IPC: B01L7/00 ,  C12Q3/00 ,  C12P19/34

CPC classification number: B01L7/52 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12P19/34 ,  C12Q3/00 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/76

Abstract: A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.