公开(公告)号：US09915670B2

公开(公告)日：2018-03-13

申请号：US15022362

申请日：2014-09-16

申请人： Nanyang Technological University ,  Agency for Science, Technology and Research

发明人： Malini Olivo ,  Kien Voon Kong ,  Weng Kee Leong

IPC分类号： C12Q1/28 ,  G01N33/84 ,  B82Y15/00 ,  G01N21/65 ,  C01G55/00 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/58

CPC分类号： G01N33/84 ,  B82Y15/00 ,  C01G55/008 ,  C12Q1/26 ,  C12Q1/54 ,  G01N21/658 ,  G01N33/587 ,  G01N2333/904

摘要： A method of detecting one or more analytes comprising or consisting of hydrogen peroxide using surface enhanced Raman spectroscopy (SERS) is provided. The method includes providing a SERS-active substrate having at least one metal carbonyl cluster compound attached thereon; contacting one or more analytes with the SERS-active substrate; and detecting changes in surface enhanced Raman signal from the at least one metal carbonyl cluster compound as an indication of the presence of one or more analytes comprising or consisting of hydrogen peroxide.

公开(公告)号：US20180037927A1

公开(公告)日：2018-02-08

申请号：US15667172

申请日：2017-08-02

申请人： ARKRAY, Inc.

发明人： Koji Katsuki ,  Takehiro Funamoto ,  Masaki Murakami

IPC分类号： C12Q1/26 ,  C12Q1/28 ,  G01N21/78 ,  C12Q1/25

CPC分类号： C12Q1/26 ,  C12Q1/25 ,  C12Q1/28 ,  C12Q1/527 ,  C12Y102/03003 ,  C12Y111/01 ,  C12Y603/01002 ,  G01N21/78 ,  G01N33/52 ,  G01N33/84 ,  G01N2021/752 ,  G01N2021/7773 ,  G01N2021/7783 ,  G01N2333/90203

摘要： A method of quantifying ammonia, the method includes performing a first reaction in which a test liquid containing ammonia is reacted with adenosine triphosphate and L-glutamic acid in the presence of glutamine synthetase to produce phosphoric acid, performing a second reaction in which the produced phosphoric acid is reacted with pyruvic acid in the presence of pyruvate oxidase, and measuring a component consumed or produced by the second reaction, to quantify ammonia, wherein a reaction to produce adenosine triphosphate from adenosine diphosphate mediated by pyruvate kinase is not carried out.

公开(公告)号：US20180024113A1

公开(公告)日：2018-01-25

申请号：US15451959

申请日：2017-03-07

申请人： Invoy Technologies, LLC

发明人： Lubna Ahmad

IPC分类号： G01N33/497 ,  C12Q1/54 ,  G01N25/48 ,  G01N33/98 ,  A61B5/097 ,  G01N33/64 ,  G01N21/71 ,  G01N21/75 ,  C12Q1/26 ,  G01N25/20 ,  G01N25/00 ,  G01N21/84 ,  G01N21/78 ,  G01N21/3504 ,  G01N21/03 ,  G01N15/02 ,  G01D1/06 ,  G01N27/26 ,  G01N31/22 ,  G01N33/68 ,  G01N1/22

CPC分类号： G01N33/497 ,  A61B5/097 ,  C12Q1/26 ,  C12Q1/54 ,  G01D1/06 ,  G01N15/0205 ,  G01N21/0332 ,  G01N21/3504 ,  G01N21/71 ,  G01N21/75 ,  G01N21/783 ,  G01N21/8483 ,  G01N25/00 ,  G01N25/20 ,  G01N25/4813 ,  G01N25/4873 ,  G01N27/26 ,  G01N31/22 ,  G01N31/224 ,  G01N33/4972 ,  G01N33/64 ,  G01N33/6893 ,  G01N33/98 ,  G01N2001/2223 ,  G01N2033/4975 ,  G01N2800/042 ,  G01N2800/347 ,  G01N2800/50 ,  Y10T436/172307 ,  Y10T436/175383 ,  Y10T436/196666 ,  Y10T436/200833 ,  Y10T436/202499 ,  Y10T436/203332 ,  Y10T436/204165 ,  Y10T436/212 ,  Y10T436/214

摘要： An apparatus is provided for sensing an analyte in a fluid. The apparatus includes a fluid collecting device configured to collect the fluid containing the analyte; a fluid input in fluid communication with the fluid collecting device configured to input the fluid containing the analyte into the fluid collecting device, an analyte interactant in fluid communication with the fluid collecting device, wherein the analyte interactant, when contacted by the analyte, reacts to cause a first change in thermal energy within the fluid collecting device; a modulator that causes a second change in thermal energy; a thermal sensing device comprising at least one pyroelectric device thermally coupled to the fluid collecting device to generate a first signal in response to at least one of the first change in thermal energy and the second change in thermal energy; a control device operatively coupled to the thermal sensing device and the modulator that generates a second signal, wherein the second signal comprises information useful in characterizing the analyte. A related method also is disclosed.

公开(公告)号：US09856508B2

公开(公告)日：2018-01-02

申请号：US15345098

申请日：2016-11-07

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Rouh-Rong Juo ,  Brooks Edwards ,  Melissa Gee ,  Zhixian Wang ,  Kathleen Skaare

IPC分类号： C12Q1/26

CPC分类号： C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

摘要： Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

公开(公告)号：US20170355967A1

公开(公告)日：2017-12-14

申请号：US15521104

申请日：2015-10-23

申请人： Kikkoman Corporation

发明人： Yosuke Masakari ,  Airi KOMATSUZAKI ,  Atsushi ICHIYANAGI

IPC分类号： C12N9/06 ,  G01N27/327 ,  C12Q1/26 ,  C12Q1/00

CPC分类号： C12N9/0032 ,  C12M1/40 ,  C12Q1/004 ,  C12Q1/005 ,  C12Q1/26 ,  C12Y105/03 ,  G01N27/327 ,  G01N27/3272 ,  G01N27/3275 ,  G01N27/416 ,  G01N2333/805 ,  G01N2333/90672

摘要： Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.

公开(公告)号：US09815811B2

公开(公告)日：2017-11-14

申请号：US14774383

申请日：2014-03-12

申请人： CURADEV PHARMA PRIVATE LTD.

发明人： Monali Banerjee ,  Sandip Middya ,  Ritesh Shrivastava ,  Sushil Raina ,  Arjun Surya ,  Dharmendra B. Yadav ,  Veejendra K. Yadav ,  Kamal Kishore Kapoor ,  Aranapakam Venkatesan ,  Roger A. Smith ,  Scott K. Thompson

IPC分类号： C07D491/048 ,  C07D333/66 ,  C07D307/82 ,  C07D491/04 ,  C07D495/04 ,  A61K31/343 ,  A61K31/381 ,  A61K31/4355 ,  A61K31/4365 ,  A61K31/4436 ,  A61K31/444 ,  A61K31/4545 ,  A61K31/5377 ,  A61K31/704 ,  C07D409/04 ,  C07D409/12 ,  C12Q1/26

CPC分类号： C07D333/66 ,  A61K31/343 ,  A61K31/381 ,  A61K31/4355 ,  A61K31/4365 ,  A61K31/4436 ,  A61K31/444 ,  A61K31/4545 ,  A61K31/5377 ,  A61K31/704 ,  C07D307/82 ,  C07D409/04 ,  C07D409/12 ,  C07D491/04 ,  C07D491/048 ,  C07D495/04 ,  C12Q1/26 ,  G01N2333/90241 ,  Y02A50/411

摘要： The present application provides novel inhibitors of indoleamine 2,3-dioxygenase-1 and/or indoleamine 2,3-dioxygenase-2 and/or tryptophan 2,3-dioxygenase, metabolites thereof, and pharmaceutically acceptable salts or prodrugs thereof. Also provided are methods for preparing these compounds. A therapeutically effective amount of one or more of the compounds of formula (I) is useful in treating diseases resulting from dysregulation of the kynurenine pathway. Compounds of formula (I) act by inhibiting the enzymatic activity or expression of indoleamine 2,3-dioxygenase-1 and/or indoleamine 2,3-dioxygenase-2 and/or tryptophan 2,3-dioxygenase.

公开(公告)号：US20170254771A1

公开(公告)日：2017-09-07

申请号：US15493482

申请日：2017-04-21

申请人： Instrumentation Laboratory Company

发明人： Shankar Balasubramanian ,  Paul D'Orazio

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/26

CPC分类号： G01N27/3272 ,  C12Q1/001 ,  C12Q1/005 ,  C12Q1/26 ,  G01N27/327 ,  G01N27/3271 ,  G01N27/3277

摘要： The present invention pertains to a hemolysis sensor, a hemolysis sensor system and methods of utilizing the hemolysis sensor or hemolysis sensor system to monitor or detect hemolysis in a sample, such as a whole blood sample, a plasma sample, a serum sample or hemolyzed blood. The hemolysis sensor responds to extracellular hemoglobin levels, for example, extracellular hemoglobin in a whole blood sample as a method for detecting hemolysis in whole blood.

公开(公告)号：US09738916B2

公开(公告)日：2017-08-22

申请号：US14895785

申请日：2014-07-07

申请人： Panasonic Healthcare Holdings Co., Ltd.

发明人： Masaki Fujiwara ,  Tomohiro Yamamoto

IPC分类号： G01N27/327 ,  C12Q1/00 ,  C12Q1/26

CPC分类号： C12Q1/006 ,  C12Q1/26 ,  G01N27/3274

摘要： Provided are a blood component measurement device and the like capable of further suppressing the errors in measuring blood components. A first current value that is generated by oxidation-reduction when a first voltage is applied to a first electrode pair 21, 22 composing a biosensor 1 is measured, a second current value that is generated when a second voltage is applied to a second electrode pair 23, 24 composing the biosensor 1 is measured, and then the first current and the second current are converted to give a blood component amount. Within a predetermined period after the introduction of blood into the biosensor 1, the first current is measured multiple times and the second current is measured once. A CPU 72 converts a plurality of first current values and the second current value to obtain a plurality of blood component amounts and calculates the blood component amount for the blood introduced into the biosensor 1 from said plurality of blood component amounts.

公开(公告)号：US20170226559A1

公开(公告)日：2017-08-10

申请号：US15514956

申请日：2015-10-01

申请人： Centre for Addiction and Mental Health

发明人： Jeffrey Meyer ,  Ken Evans ,  Jian Chen ,  Suzanne Ackloo ,  Moyez Dharsee

IPC分类号： C12Q1/26 ,  A61B5/145 ,  C40B40/10 ,  C12N9/06 ,  G01N33/50 ,  C12Q1/68 ,  A61B5/11

CPC分类号： C12Q1/26 ,  A61B5/1127 ,  A61B5/145 ,  C07K7/06 ,  C07K7/08 ,  C12N9/0022 ,  C12Q1/68 ,  C12Y104/03004 ,  C40B40/10 ,  G01N33/5008 ,  G01N2333/90638 ,  G01N2560/00 ,  G01N2800/304

摘要： Disclosed is a method for diagnosing a mood disorder or susceptibility to a mood disorder, including depressive disorders and bipolar disorder, from a biological sample taken from a subject. The method includes detecting markers of monoamine oxidase-A (MAO-A) in the biological sample; determining MAO-A concentration from the markers; and correlating the MAO-A concentration in the biological sample to a control group which does not have a mood disorder in order to diagnose or determine susceptibility to the mood disorder in the subject. Also disclosed is a method of detecting peripheral markers of MAO-A for the diagnosis of a mood disorder or susceptibility to a mood disorder. Also provided are polypeptide markers.

公开(公告)号：US20170205393A1

公开(公告)日：2017-07-20

申请号：US15320710

申请日：2015-10-07

申请人： Qualyst Transporter Solutions, LLC

发明人： Kenneth R. Brouwer ,  Christopher Black ,  Jonathan Jackson

IPC分类号： G01N33/50 ,  C12Q1/26

CPC分类号： G01N33/5008 ,  C12Q1/26 ,  G01N33/5067 ,  G01N2500/10

摘要： A method of evaluating disposition and/or effect of a candidate compound in an in vitro culture and/or suspension to predict in vivo disposition and/or effect of the candidate compound, which can include the steps of providing a cell culture and/or suspension; exposing a candidate compound to the culture and/or suspension; exposing the culture and/or suspension to a media providing an in vivo relevant extracellular environment, such as a media comprising a component (such as a protein and/or other component, such as lipoproteins, bile acids, and endogenous compounds such as bilirubin) at a physiologic concentration or a concentration having characteristics, such as binding characteristics, similar to the physiological concentration; wherein any combination of any of the exposing steps can occur in any order or simultaneously; and evaluating in vitro disposition and/or effect of the compound to predict in vivo disposition and/or effect of the candidate compound.