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公开(公告)号：US11255809B2
公开(公告)日：2022-02-22
申请号：US16788187
申请日：2020-02-11
IPC分类号： G01N27/26 , B01F13/00 , B01L3/00 , B01L7/00 , G01N35/00 , C12Q1/686 , C12Q1/6869 , G01N27/447 , G01N27/327 , G01N27/416 , B01L3/02
摘要： The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.
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公开(公告)号：US10809254B2
公开(公告)日：2020-10-20
申请号：US16191270
申请日：2018-11-14
发明人： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen E. Eckhardt
IPC分类号： G01N1/40 , G01N1/00 , G01N1/10 , G01N1/22 , G01N1/18 , G01N35/00 , G01N1/38 , B01L3/00 , G01N33/543 , B01F11/00 , B01F13/00 , B01F13/08 , B01L7/00 , G01N35/10
摘要： Provided herein are methods of splitting droplets containing magnetically responsive beads in a droplet actuator. A droplet actuator having a plurality of droplet operations electrodes configured to transport the droplet, and a magnetic field present at the droplet operations electrodes, is provided. The magnetically responsive beads in the droplet are immobilized using the magnetic field and the plurality of droplet operations electrodes are used to split the droplet into first and second droplets while the magnetically responsive beads remain substantially immobilized.
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公开(公告)号：US10379112B2
公开(公告)日：2019-08-13
申请号：US14719731
申请日：2015-05-22
发明人： Ramakrishna Sista , Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Allen E. Eckhardt , Richard B. Fair
IPC分类号： B01L3/00 , G01N27/447 , G01N33/543 , C12M1/12 , G01N35/10 , G01N35/00
摘要： A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.
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公开(公告)号：US10078078B2
公开(公告)日：2018-09-18
申请号：US15210634
申请日：2016-07-14
IPC分类号： G01N15/06 , G01N33/00 , G01N33/48 , G01N33/543 , B01F11/00 , B01F13/00 , B01L3/00 , B03C1/02 , B03C1/30 , B03C5/00 , G01N33/561 , B03C1/28 , C12N15/10 , B01L7/00 , G01N35/10
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
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公开(公告)号：US10005080B2
公开(公告)日：2018-06-26
申请号：US14538506
申请日：2014-11-11
发明人： Jon Faiz Kayyem , Sean Ford , Vamsee K. Pamula , Vijay Srinivasan , Scott Corey , Brian Murphy , David Walter Wright , Dominic Aiello
CPC分类号： B01L3/502 , B01L3/502792 , B01L3/52 , B01L7/525 , B01L2200/04 , B01L2200/0647 , B01L2200/10 , B01L2200/16 , B01L2300/021 , B01L2300/04 , B01L2300/06 , B01L2300/0636 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1822 , B01L2400/0427 , B01L2400/043 , B01L2400/0481 , B01L2400/0487 , B01L2400/0683
摘要： In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.
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公开(公告)号：US09952177B2
公开(公告)日：2018-04-24
申请号：US14795304
申请日：2015-07-09
发明人： Michael G. Pollack , Vijay Srinivasan , Zhishan Hua , Hon Lung Chu , Michael Hauser , Allen Eckhardt
IPC分类号： G01N27/447 , G01N27/453 , B01L3/00 , B01L7/00
CPC分类号： G01N27/44743 , B01L3/502715 , B01L3/502792 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2300/088 , B01L2300/0887 , B01L2300/089 , B01L2300/161 , B01L2400/0406 , B01L2400/043 , B01L2400/0457 , B01L2400/046 , G01N27/44791
摘要： The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.
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公开(公告)号：US09861986B2
公开(公告)日：2018-01-09
申请号：US14506265
申请日：2014-10-03
发明人： Michael G. Pollack , Prasanna Thwar , Vamsee K. Pamula , Vijay Srinivasan , Allen E. Eckhardt , Alexander Shenderov , Dwayne J. Allen
IPC分类号： B01L3/00 , G01N27/447 , B01L7/00 , G01N35/10 , G01N35/00
CPC分类号： B01L3/502784 , B01L3/502715 , B01L3/502761 , B01L7/52 , B01L2200/027 , B01L2200/0673 , B01L2300/022 , B01L2300/023 , B01L2300/024 , B01L2300/0672 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/049 , B01L2400/0683 , G01N27/44704 , G01N35/0098 , G01N2035/1046 , Y10T436/25 , Y10T436/2575
摘要： The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.
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8.发明授权Method and device for conducting biochemical or chemical reactions at multiple temperatures 有权在多个温度下进行生化或化学反应的方法和装置公开(公告)号：US09517469B2
公开(公告)日：2016-12-13
申请号：US11912913
申请日：2006-05-10
CPC分类号： B01L7/525 , B01L3/502792 , B01L2200/0673 , B01L2300/0654 , B01L2300/0864 , B01L2300/0887 , B01L2300/089 , B01L2300/1816 , B01L2300/1827 , B01L2300/1872 , B01L2400/0427
摘要： Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones.
摘要翻译： 描述了需要多个反应温度的用于进行化学或生化反应的方法和装置。 所述方法包括在微流体装置上移动一个或多个反应液滴或反应体积通过具有不同温度的各种反应区。 这些装置包括微流体装置，其包括能够通过各种反应区移动反应液滴或反应体积的适当致动器。
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公开(公告)号：US09511369B2
公开(公告)日：2016-12-06
申请号：US14248884
申请日：2014-04-09
IPC分类号： B01L3/00
CPC分类号： B01L3/502792 , B01L2200/12 , B01L2300/0819 , B01L2300/089 , B01L2400/0415 , B01L2400/0421 , B01L2400/0427 , Y10T29/49826
摘要： The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.
摘要翻译： 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。
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公开(公告)号：US20160320378A1
公开(公告)日：2016-11-03
申请号：US15210634
申请日：2016-07-14
IPC分类号： G01N33/543 , C12N15/10 , B03C1/30 , B03C5/00 , B03C1/28 , G01N33/561 , B01L3/00
CPC分类号： G01N33/54333 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01L3/502715 , B01L3/502761 , B01L3/502784 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0668 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , B03C1/02 , B03C1/288 , B03C1/30 , B03C5/005 , B03C2201/18 , B03C2201/26 , C12N15/1013 , G01N33/561 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
摘要： The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.
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