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    Method and apparatus determining the isoelectric point of charged analyte
    1.
    发明授权
    Method and apparatus determining the isoelectric point of charged analyte 失效
    测定带电分析物等电点的方法和装置

    公开(公告)号：US08080145B2

    公开(公告)日：2011-12-20

    申请号：US10542493

    申请日：2004-01-12

    申请人： David Strand ,  Dan M. Leatzow

    发明人： David Strand ,  Dan M. Leatzow

    IPC分类号： G01N27/447

    CPC分类号： B01D61/425 ,  C07K1/28 ,  G01N27/44704

    摘要： Devices are provided for determining the isoelectric point of a charged analyte, comprising a titration chamber and an electrode chamber. The electrode chamber comprises at least two electrodes, for example, an electrode array. Either or both of the titration chamber and the electrode chamber may have a shaped geometry. The electrodes are operative, in conjunction with the shaped geometry of the chamber(s) where appropriate, to generate an electric field gradient in the titration chamber. Permeable material separates the titration chamber and the electrode chamber. A pH Sensor is located in the titration chamber for obtaining the pH of the first fluid. Certain preferred embodiments further include an analyte band detector for detecting the presence and optionally the location of a focused band of charged solute. Methods are provided for determining the isoelectric point of a charged analyte comprising introducing a carrier fluid comprising a Charge analyte into the titration chamber of a device as just described and applying an electric field gradient to focus the charged analyte into a focused band. The pH of the carrier fluid is incremented or adjusted to shift the location of the focused band of charged analyte, and the pH and location of the focused band of charged analyte are obtained for a plurality of locations and pH's and the isoelectric point is determined from such data.

    摘要翻译： 提供了用于确定带电分析物的等电点的装置，包括滴定室和电极室。 电极室包括至少两个电极，例如电极阵列。 滴定室和电极室中的任一个或两者可以具有成形几何形状。 电极在适当的情况下与室的成形几何结合起作用，以在滴定室中产生电场梯度。 渗透材料分离滴定室和电极室。 pH传感器位于滴定室中以获得第一流体的pH。 某些优选实施例还包括用于检测带电溶质的聚焦带的存在和可选地位置的分析物带检测器。 提供了用于确定带电分析物的等电点的方法，包括将包含电荷分析物的载体流体引入如上所述的装置的滴定室中，并施加电场梯度以将带电分析物聚焦到聚焦带中。 载体流体的pH被递增或调整以移动带电分析物的聚焦带的位置，并且对于多个位置和pH值获得带电分析物的聚焦带的pH和位置，并且等电点由 这样的数据。

    Electrophoresis Devices And Methods For Focusing Charged Analytes
    2.
    发明申请
    Electrophoresis Devices And Methods For Focusing Charged Analytes 有权
    电泳装置和聚焦电荷分析物的方法

    公开(公告)号：US20080087546A1

    公开(公告)日：2008-04-17

    申请号：US10557582

    申请日：2004-05-19

    申请人： David Strand ,  Dan M. Leatzow ,  Cornelius F. Ivory

    发明人： David Strand ,  Dan M. Leatzow ,  Cornelius F. Ivory

    IPC分类号： G01N27/447 ,  G01N27/26

    CPC分类号： G01N27/44795

    摘要： Devices are provided for separating and focusing charged analytes, comprising a separation chamber and two or more electrodes, for example, an electrode array. A membrane separates the separation chamber and the electrodes. The separation chamber of the device is configured, that is, the separation chamber has a shaped geometry, which serves to induce a gradient in an electric field generated by the electrodes in the electrode chamber. Optionally, molecular sieve is included in the separation chamber that is operative to shift the location at which a stationary focused band of a charged analyte forms under a given set of focusing process parameters. Methods are provided for separating and focusing charged analytes comprising introducing a first fluid comprising at least one charged analyte into the separation chamber of a device as just described, applying an electric field gradient to the charged analyte to focus the charged analyte in the electric field gradient.

    摘要翻译： 提供了用于分离和聚焦带电分析物的装置，包括分离室和两个或更多个电极，例如电极阵列。 膜分离室和电极。 该装置的分离室被构造成即分离室具有成形几何形状，其用于在电极室中由电极产生的电场中引起梯度。 任选地，分子筛包括在分离室中，其可操作以在给定的一组聚焦工艺参数下移动带电分析物的固定聚焦带的位置。 提供了用于分离和聚焦带电分析物的方法，包括将如上所述将包含至少一种带电分析物的第一流体引入到器件的分离室中，对电荷分析物施加电场梯度以将带电分析物聚焦在电场梯度 。

    Steep solvent gradient NMR analysis method
    4.
    发明申请
    Steep solvent gradient NMR analysis method 失效
    陡溶剂梯度NMR分析方法

    公开(公告)号：US20020105327A1

    公开(公告)日：2002-08-08

    申请号：US10006503

    申请日：2001-12-03

    申请人： Protasis Corporation

    发明人： Tim L. Peck ,  Dean Olson ,  Jim Norcross

    IPC分类号： G01V003/00

    CPC分类号： G01R33/302 ,  G01R33/307 ,  G01R33/46 ,  G01R33/5676

    摘要： An NMR method of analyzing an analyte comprises feeding an analyte sample fluid to an NMR flow cell. The NMR flow cell comprises an RF microcoil operably associated with an enlarged containment region. The mobile phase of the analyte sample flowing through the NMR flow cell has a solvent gradient greater than 10% per minute. The analyte sample fluid can be fed to the NMR flow cell from an analyte extraction chamber, e.g., operative to perform liquid chromatography, capillary electrophoresis, or the like, especially a capillary-based analyte extraction chamber integrated in an NMR probe with the NMR flow cell. A sample volume is held in the NMR flow cell for equilibration less than 1 hour, preferably less than 30 minutes prior to actuating NMR analysis of the observe volume in the microcoil.

    摘要翻译： 分析分析物的NMR方法包括将分析物样品流体进料到NMR流动池。 NMR流通池包括可操作地与扩大的容纳区域相关联的RF微线圈。 流过NMR流通池的分析物样品的流动相具有大于10％/分钟的溶剂梯度。 分析物样品流体可以从分析物提取室进料至NMR流动池，例如，可操作以进行液相色谱，毛细管电泳等，特别是集成在具有NMR流量的NMR探针中的基于毛细管的分析物提取室 细胞。 样品体积保存在NMR流通池中，用于平衡小于1小时，优选小于30分钟，然后在微量线圈上观察体积的NMR分析。

    Method and device for ultrasonically manipulating particles within a fluid
    5.
    发明授权
    Method and device for ultrasonically manipulating particles within a fluid 失效
    用于超声波操纵流体内颗粒的方法和装置

    公开(公告)号：US07846382B2

    公开(公告)日：2010-12-07

    申请号：US10516599

    申请日：2003-06-03

    申请人： David Strand ,  David Barrow ,  Joseph Cefai

    发明人： David Strand ,  David Barrow ,  Joseph Cefai

    IPC分类号： C02F1/32

    CPC分类号： B01D21/283 ,  B01D49/006 ,  B01D51/08 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2400/0433 ,  B01L2400/0439 ,  B01L2400/086 ,  G01N2015/0288 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/149

    摘要： Fluid-handling methods and devices for ultrasonic manipulation of fluid-borne particles comprise a fluid-handling manifold and an ultrasonic particle manipulator defining an ultrasonic cavity within the manifold. Fluid-borne particles introduced into the manifold are manipulated by controlling ultrasonic standing waves at the ultrasonic cavity. Cavities having non-uniform configurations, asymmetric standing waves and/or multiple ultrasonic cavities within the manifold are operative to control the movement of the fluid-borne particles, optionally including collecting and holding such particles, transferring particles through an intersection from one channel to another, etc. Solid phase extraction (SPE) particles, biological particles and other fluid-borne particles can be manipulated within the fluid-handling manifold.

    摘要翻译： 用于流体传播颗粒的超声操作的流体处理方法和装置包括在歧管内限定超声波腔的流体处理歧管和超声波粒子操纵器。 引入到歧管中的流体颗粒通过在超声腔处控制超声波驻波来操纵。 在歧管内具有不均匀构型，不对称驻波和/或多个超声腔的腔可用于控制流体传播颗粒的运动，任选地包括收集和保持这种颗粒，将颗粒从一个通道转移到另一个通道 固相萃取（SPE）颗粒，生物颗粒和其它流体传导的颗粒可在流体处理歧管内操纵。

    Method and device for ultrasonically manipulating particles within a fluid
    7.
    发明申请
    Method and device for ultrasonically manipulating particles within a fluid 失效
    用于超声波操纵流体内颗粒的方法和装置

    公开(公告)号：US20060037915A1

    公开(公告)日：2006-02-23

    申请号：US10516599

    申请日：2003-06-03

    申请人： David Strand ,  David Barrow ,  Joseph Cefai

    发明人： David Strand ,  David Barrow ,  Joseph Cefai

    IPC分类号： C02F1/32

    CPC分类号： B01D21/283 ,  B01D49/006 ,  B01D51/08 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2400/0433 ,  B01L2400/0439 ,  B01L2400/086 ,  G01N2015/0288 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/149

    摘要： Fluid-handling methods and devices for ultrasonic manipulation of fluid-borne particles comprise a fluid-handling manifold and an ultrasonic particle manipulator defining an ultrasonic cavity within the manifold. Fluid-borne particles introduced into the manifold are manipulated by controlling ultrasonic standing waves at the ultrasonic cavity. Cavities having non-uniform configurations, asymmetric standing waves and/or multiple ultrasonic cavities within the manifold are operative to control the movement of the fluid-borne particles, optionally including collecting and holding such particles, transferring particles through an intersection from one channel to another, etc. Solid phase extraction (SPE) particles, biological particles and other fluid-borne particles can be manipulated within the fluid-handling manifold.

    摘要翻译： 用于流体传播颗粒的超声操作的流体处理方法和装置包括在歧管内限定超声波腔的流体处理歧管和超声波粒子操纵器。 引入到歧管中的流体颗粒通过在超声腔处控制超声波驻波来操纵。 在歧管内具有不均匀构型，不对称驻波和/或多个超声腔的腔可用于控制流体传播颗粒的运动，任选地包括收集和保持这种颗粒，将颗粒从一个通道转移到另一个通道 固相萃取（SPE）颗粒，生物颗粒和其它流体传导的颗粒可在流体处理歧管内操纵。

    Vortex-stabilized electrophoretic devices and methods
    9.
    发明授权
    Vortex-stabilized electrophoretic devices and methods 失效
    涡旋稳定电泳装置及方法

    公开(公告)号：US07938946B2

    公开(公告)日：2011-05-10

    申请号：US10544511

    申请日：2004-02-06

    申请人： Cornelius Ivory ,  Noah Tracy ,  Dan M. Leatzow

    发明人： Cornelius Ivory ,  Noah Tracy ,  Dan M. Leatzow

    IPC分类号： C02F1/38 ,  G01N27/00

    CPC分类号： G01N27/44795 ,  C07K1/26

    摘要： A vortex-stabilized electrophoretic processor comprises an annular processing chamber at least partly defined by concentric first and second processing chamber surfaces. At least one of the processing chamber surfaces is rotatable relative to the other processing chamber surface. The electrophoretic processor further comprises an electric field generator operative to be energized to establish a dynamic field gradient within the processing chamber. At least one fluid port is provided, having fluid communication with the processing chamber. The electric field generator may comprise an elongate electrode array positioned within a central bore of a rotor forming the inside surface of the processing chamber. One or both of the processing chamber surfaces can have shaping comprising multiple spaced tines or annular ridges extending toward the other of the processing chamber surfaces.

    摘要翻译： 涡流稳定的电泳处理器包括至少部分地由同心的第一和第二处理室表面限定的环形处理室。 处理室表面中的至少一个可相对于另一个处理室表面旋转。 电泳处理器还包括电场发生器，其操作以被激励以在处理室内建立动态场梯度。 提供至少一个流体端口，其具有与处理室的流体连通。 电场发生器可以包括定位在形成处理室的内表面的转子的中心孔内的细长电极阵列。 处理室表面中的一个或两个可以具有包括朝向另一个处理室表面延伸的多个间隔开的齿或环形脊的成形。