公开(公告)号：US20050076396A1

公开(公告)日：2005-04-07

申请号：US10651564

申请日：2003-08-29

申请人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu ,  Patricia Zuk ,  Peter Ashjian

发明人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu ,  Patricia Zuk ,  Peter Ashjian

IPC分类号： A61K35/12 ,  A61K48/00 ,  C12N5/00 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0789 ,  A01K67/00 ,  C12N5/08

CPC分类号： C12N5/0653 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0068 ,  C12N5/0607 ,  C12N5/0647 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0658 ,  C12N5/0667 ,  C12N9/1276 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/01 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2510/00 ,  C12N2533/90 ,  C12Y207/07049

摘要： The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

公开(公告)号：US20050153441A1

公开(公告)日：2005-07-14

申请号：US10740315

申请日：2003-12-17

申请人： Marc Hedrick ,  Adam Katz ,  Ramon Llull ,  J. Futrell ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu

发明人： Marc Hedrick ,  Adam Katz ,  Ramon Llull ,  J. Futrell ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu

IPC分类号： C12N15/09 ,  A61K35/12 ,  A61K48/00 ,  A61L27/00 ,  C12M3/08 ,  C12N5/00 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0789 ,  C12N5/10 ,  C12N5/08

CPC分类号： C12N5/0667 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0068 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/01 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2510/00 ,  C12N2533/90

摘要： The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

公开(公告)号：US20050282275A1

公开(公告)日：2005-12-22

申请号：US11211114

申请日：2005-08-24

申请人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu

发明人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu

IPC分类号： C12N5/071 ,  A61K35/12 ,  A61K48/00 ,  C12N5/00 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0789 ,  C12N5/10 ,  C12N15/63 ,  C12N5/08

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0068 ,  C12N5/0647 ,  C12N5/0652 ,  C12N5/0653 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0667 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/01 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2502/1305 ,  C12N2506/1384 ,  C12N2510/00 ,  C12N2533/90

摘要： The present invention provides adipose-derived stem cells and lattices. In one aspect, the present invention provides a lipo-derived stem cell substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The invention also provides a method of isolating stem cells from adipose tissues. The cells can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the cells can be expanded and cultured to produce hormones and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a lipo-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

摘要翻译： 本发明提供脂肪来源的干细胞和晶格。 一方面，本发明提供了一种基本上不含脂肪细胞和结缔组织干细胞的红细胞和克隆群的脂衍生的干细胞。 本发明还提供了从脂肪组织分离干细胞的方法。 细胞可以单独使用或在生物相容性组合物内使用，以在体内和体外产生分化的组织和结构。 此外，细胞可以扩增和培养以产生激素，并提供条件培养基以支持其他细胞群体的生长和扩增。 另一方面，本发明提供了一种基本上不含细胞的脂衍生晶格，其包括来自脂肪组织的细胞外基质材料。 晶格可以用作底物，以促进细胞的生长和分化，无论是在体内还是在体外，成为成体组织或甚至成熟的组织或结构。

公开(公告)号：US20050153442A1

公开(公告)日：2005-07-14

申请号：US10845315

申请日：2004-05-12

申请人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu ,  Patricia Zuk ,  Peter Ashjian

发明人： Adam Katz ,  Ramon Llull ,  J. Futrell ,  Marc Hedrick ,  Prosper Benhaim ,  Hermann Lorenz ,  Min Zhu ,  Patricia Zuk ,  Peter Ashjian

IPC分类号： A61K35/12 ,  A61K48/00 ,  A61L27/22 ,  A61L27/38 ,  C12N5/00 ,  C12N5/074 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/0789 ,  C12N15/85 ,  C12N5/08

CPC分类号： A61L27/3612 ,  A61K35/12 ,  A61K48/00 ,  A61L27/225 ,  A61L27/3654 ,  A61L27/38 ,  C12N5/0068 ,  C12N5/0607 ,  C12N5/0647 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0658 ,  C12N5/0667 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/42 ,  C12N2501/01 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2502/1305 ,  C12N2510/00 ,  C12N2533/90

摘要： The present invention provides adipose-derived stem cells (ADSCs), adipose-derived stem cell-enriched fractions (ADSC-EF) and adipose-derived lattices, alone and combined with the ADSCs of the invention. In one aspect, the present invention provides an ADSC substantially free of adipocytes and red blood cells and clonal populations of connective tissue stem cells. The ADSCs can be employed, alone or within biologically-compatible compositions, to generate differentiated tissues and structures, both in vivo and in vitro. Additionally, the ADSCs can be expanded and cultured to produce molecules such as hormones, and to provide conditioned culture media for supporting the growth and expansion of other cell populations. In another aspect, the present invention provides a adipose-derived lattice substantially devoid of cells, which includes extracellular matrix material from adipose tissue. The lattice can be used as a substrate to facilitate the growth and differentiation of cells, whether in vivo or in vitro, into anlagen or even mature tissues or structures.

摘要翻译： 本发明单独提供脂肪来源的干细胞（ADSCs），脂肪来源的干细胞富含级分（ADSC-EF）和脂肪衍生的格子，并与本发明的ADSC组合。 一方面，本发明提供了基本上不含结缔组织干细胞的脂肪细胞和红细胞和克隆群体的ADSC。 ADSC可以单独使用或在生物相容性组合物内使用，以在体内和体外产生分化的组织和结构。 另外，可以扩增和培养ADSC以产生分子如激素，并提供条件培养基以支持其它细胞群的生长和扩增。 另一方面，本发明提供了一种基本上不含细胞的脂肪衍生的晶格，其包括来自脂肪组织的细胞外基质材料。 晶格可以用作底物，以促进细胞的生长和分化，无论是在体内还是在体外，成为成体组织或甚至成熟的组织或结构。

公开(公告)号：US20050008626A1

公开(公告)日：2005-01-13

申请号：US10783957

申请日：2004-02-20

申请人： John Fraser ,  Marc Hedrick ,  Min Zhu ,  Brian Strem ,  Eric Daniels ,  Isabella Wulur

发明人： John Fraser ,  Marc Hedrick ,  Min Zhu ,  Brian Strem ,  Eric Daniels ,  Isabella Wulur

IPC分类号： A61K35/12 ,  A61K35/28 ,  A61K35/34 ,  A61K35/36 ,  A61K35/44 ,  C12N5/071 ,  C12N5/0775 ,  A61K48/00 ,  C12N5/08

CPC分类号： A61K35/28 ,  A61K35/34 ,  A61K35/44 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/54 ,  A61L2300/414 ,  A61L2430/36 ,  C12N5/0667 ,  C12N5/069 ,  C12N2506/1384

摘要： Cells present in processed lipoaspirate tissue are used to treat patients, including patients with cardiovascular conditions, diseases or disorders. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, cells present in processed lipoaspirate are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic cardiovascular benefit.

摘要翻译： 存在于加工的脂肪抽吸组织中的细胞用于治疗患者，包括患有心血管病症，疾病或病症的患者。 治疗患者的方法包括加工脂肪组织以将从脂肪组织获得的浓缩量的干细胞输送给患者。 所述方法可以在封闭系统中实施，使得干细胞在施用于患者之前不暴露于外部环境。 因此，在优选的方法中，存在于加工的lipoaspirate中的细胞与用于促进，产生或支持治疗性心血管益处所必需的这种添加剂一起直接放置在受体中。

公开(公告)号：US20060083720A1

公开(公告)日：2006-04-20

申请号：US11138083

申请日：2005-05-25

申请人： John Fraser ,  Marc Hedrick ,  Min Zhu ,  Brian Strem ,  Eric Daniels ,  Isabella Wulur

发明人： John Fraser ,  Marc Hedrick ,  Min Zhu ,  Brian Strem ,  Eric Daniels ,  Isabella Wulur

IPC分类号： A61K35/12 ,  C12N5/08

CPC分类号： A61K45/06 ,  A61K35/28 ,  C12N5/0653 ,  A61K2300/00

摘要： Adipose derived regenerative cells are used to treat patients, including patients with cardiovascular conditions, diseases or disorders. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of regenerative cells, e.g., stem and/or progenitor cells, obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Accordingly, in a preferred method, adipose derived regenerative cells are placed directly into a recipient along with such additives necessary to promote, engender or support a therapeutic cardiovascular benefit.

摘要翻译： 脂肪来源的再生细胞用于治疗患者，包括患有心血管疾病，疾病或病症的患者。 治疗患者的方法包括加工脂肪组织以将从脂肪组织获得的浓缩量的再生细胞例如干细胞和/或祖细胞递送给患者。 所述方法可以在封闭系统中实施，使得干细胞在施用于患者之前不暴露于外部环境。 因此，在优选的方法中，将脂肪来源的再生细胞与促进，产生或支持治疗性心血管益处所必需的这些添加剂一起直接放入受体中。

公开(公告)号：US20050260175A1

公开(公告)日：2005-11-24

申请号：US10884638

申请日：2004-07-02

申请人： Marc Hedrick ,  John Fraser ,  Michael Schulzki ,  Bobby Byrnes ,  Grace Carlson ,  Ronda Schreiber ,  Isabella Wulur

发明人： Marc Hedrick ,  John Fraser ,  Michael Schulzki ,  Bobby Byrnes ,  Grace Carlson ,  Ronda Schreiber ,  Isabella Wulur

IPC分类号： A61K35/12 ,  A61K35/28 ,  A61K35/36 ,  C12N5/08 ,  A61K45/00 ,  C12Q1/00

CPC分类号： C12M47/04 ,  A61B17/00 ,  A61K35/28 ,  A61M1/00 ,  C12M29/04 ,  C12M45/00 ,  C12N5/0667

摘要： Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject.

摘要翻译： 描述了用于从多种组织分离细胞的系统和方法。 特别地，描述了从脂肪组织分离再生细胞例如干细胞和/或祖细胞的自动化系统和方法。 本文所述的系统和方法提供了快速且可靠的分离和浓缩适于再次输注到受试者中的再生细胞的方法。

公开(公告)号：US20060204556A1

公开(公告)日：2006-09-14

申请号：US11317422

申请日：2005-12-22

申请人： Eric Daniels ,  Brian Strem ,  Susan Riley ,  Mike De Emedio ,  John Fraser ,  Marc Hedrick

发明人： Eric Daniels ,  Brian Strem ,  Susan Riley ,  Mike De Emedio ,  John Fraser ,  Marc Hedrick

IPC分类号： A61K35/12 ,  A61F2/06 ,  A61K9/70

CPC分类号： A61F2/82 ,  A61F2250/0067 ,  A61K35/44 ,  A61L27/18 ,  A61L27/3839 ,  A61L27/58 ,  A61L31/005 ,  A61L31/06 ,  A61L31/148 ,  C08L67/04

摘要： Cell-loaded devices or prostheses having various applications such as insertion into body passages are disclosed. The prostheses include cell carrier portions which are compatible with living tissue and which are loaded with therapeutic cell populations, and the prostheses can be applied within or replace one or more of narrow segments, environments which may be difficult to access or luminal areas of the body such as parts of blood vessels. In the context of blood vessels, the cell-loaded devices or prostheses can line or otherwise treat with therapeutic cell populations inner walls of damaged blood vessels and surrounding parenchyma or other organs.

摘要翻译： 公开了具有诸如插入到体通道中的各种应用的装有电池的装置或假体。 假体包括与活组织相容并且装有治疗细胞群体的细胞载体部分，并且假体可以施用于狭窄部分或可能难以进入的环境或身体的腔区域中的一个或多个 如血管部分。 在血管的背景下，细胞负载装置或假体可以与治疗细胞群体受损的血管和周围的实质或其他器官的内壁相连或以其他方式治疗。

公开(公告)号：US20050260174A1

公开(公告)日：2005-11-24

申请号：US10614648

申请日：2003-07-07

申请人： John Fraser ,  Marc Hedrick

发明人： John Fraser ,  Marc Hedrick

IPC分类号： A61M1/00 ,  A61K31/436 ,  A61K35/02 ,  A61K35/12 ,  A61K35/28 ,  A61K35/35 ,  A61K35/36 ,  A61K38/00 ,  A61K38/16 ,  A61K45/00 ,  A61L27/00 ,  A61P1/00 ,  A61P1/16 ,  A61P3/00 ,  A61P3/10 ,  A61P9/10 ,  A61P11/00 ,  A61P13/12 ,  A61P17/00 ,  A61P19/00 ,  A61P19/02 ,  A61P21/00 ,  A61P25/00 ,  A61P27/02 ,  A61P37/06 ,  C12M3/06 ,  C12N5/0775 ,  A01N63/00 ,  A01N65/00

CPC分类号： A61K35/28 ,  A61B10/0283 ,  A61K9/0019 ,  A61K35/35 ,  A61M1/0039 ,  A61M2202/08 ,  C12M47/04 ,  C12N5/0667 ,  C12N5/069 ,  C12N2506/1384 ,  G06F19/00

摘要： Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.

摘要翻译： 存在于加工的lipoaspirate组织中的细胞用于治疗患者。 治疗患者的方法包括加工脂肪组织以将从脂肪组织获得的浓缩量的干细胞输送给患者。 所述方法可以在封闭系统中实施，使得干细胞在施用于患者之前不暴露于外部环境。 施用于患者的组合物包括脂肪组织和干细胞的混合物，使得组合物具有比从患者中除去脂肪组织时更高浓度的干细胞。

公开(公告)号：US20050084961A1

公开(公告)日：2005-04-21

申请号：US10877822

申请日：2004-06-25

申请人： Marc Hedrick ,  John Fraser ,  Michael Schulzki ,  Bobby Byrnes ,  Grace Carlson

发明人： Marc Hedrick ,  John Fraser ,  Michael Schulzki ,  Bobby Byrnes ,  Grace Carlson

IPC分类号： C12M1/00 ,  C12N5/08

CPC分类号： A61K35/28

摘要： Systems and methods are described that are used to separate cells from a wide variety of tissues. In particular, automated systems and methods are described that separate regenerative cells, e.g., stem and/or progenitor cells, from adipose tissue. The systems and methods described herein provide rapid and reliable methods of separating and concentrating regenerative cells suitable for re-infusion into a subject.

摘要翻译： 描述了用于从多种组织分离细胞的系统和方法。 特别地，描述了从脂肪组织分离再生细胞例如干细胞和/或祖细胞的自动化系统和方法。 本文所述的系统和方法提供了快速且可靠的分离和浓缩适于再次输注到受试者中的再生细胞的方法。