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    Coating process for microfluidic sample arrays
    6.
    发明申请
    Coating process for microfluidic sample arrays 审中-公开
    微流体样品阵列的涂布工艺

    公开(公告)号：US20060105453A1

    公开(公告)日：2006-05-18

    申请号：US11198882

    申请日：2005-08-04

    申请人： Colin Brenan ,  Jamie Cho ,  Javier Garcia ,  Robert Hess ,  Tanya Kanigan ,  Arrin Katz ,  Namyong Kim ,  John Linton ,  Shailesh Srivastava ,  Karl Yoder

    发明人： Colin Brenan ,  Jamie Cho ,  Javier Garcia ,  Robert Hess ,  Tanya Kanigan ,  Arrin Katz ,  Namyong Kim ,  John Linton ,  Shailesh Srivastava ,  Karl Yoder

    IPC分类号： C12N5/00

    CPC分类号： B01J19/0046 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00659 ,  B01L3/0244 ,  B01L3/5025 ,  B01L3/50857 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/16

    摘要： A differentially coated device for conducting a plurality of nano-volume specified reactions, the device comprising a platen having at least one exterior surface modified to a specified physicochemical property, a plurality of nano-volume channels, each nano-volume channel having at least one interior surface in communication with the at least one exterior surface that is selectively coated with an optionally dissolvable coating agent physisorbed to at least one interior surface, wherein the optionally dissolvable coating agent comprises a coating agent and a first component for the plurality of specified reactions. Methods for preparing and using such devices are also provided, as well as a method of registering a location of a dispenser array in relation to a microfluidic array. A first one of the dispenser array and the microfluidic array is movable in relation to the frame, and the other of the first one of the dispenser array and the microfluidic array is fixed relative to the frame. Quantities related to a vector displacement from the alignment position to a fixed position on the one of the dispenser array and the microfluidic array is determined. The quantities thus determined are used to guide positioning of the dispenser array relative to the microfluidic array.

    摘要翻译： 一种用于进行多个纳米体积规定的反应的差异涂覆的装置，所述装置包括具有至少一个被修饰至指定理化特性的外表面的压板，多个纳米体积通道，每个纳米体积通道具有至少一个 与所述至少一个外表面连通的内表面，其被选择性地涂覆有物理吸附到至少一个内表面上的任选可溶解的涂覆剂，其中所述任选可溶解的涂覆剂包含涂层剂和用于多个特定反应的第一组分。 还提供了用于制备和使用这种装置的方法，以及相对于微流体阵列登记分配器阵列的位置的方法。 分配器阵列和微流体阵列中的第一个可相对于框架移动，并且分配器阵列和微流体阵列中的第一个中的另一个相对于框架是固定的。 确定与分配器阵列和微流体阵列之一上的对准位置到固定位置的矢量位移有关的量。 这样确定的量用于引导分配器阵列相对于微流体阵列的定位。

    Methods for enhanced protein production
    7.
    发明授权
    Methods for enhanced protein production 有权
    增强蛋白质生产的方法

    公开(公告)号：US08765415B2

    公开(公告)日：2014-07-01

    申请号：US13510372

    申请日：2010-11-17

    申请人： Alahari Arunakumari ,  Xiao-Ping Dai ,  Javier Garcia ,  Richard P. Martel

    发明人： Alahari Arunakumari ,  Xiao-Ping Dai ,  Javier Garcia ,  Richard P. Martel

    IPC分类号： C12P21/02 ,  A61K38/00

    CPC分类号： C07K1/30 ,  C07K16/00 ,  C07K2317/14 ,  C12N1/02 ,  C12P21/00 ,  C12P21/02

    摘要： The present invention provides a method of increasing protein production in a cell culture by growing cells that produce the protein (e.g., the growth phase) in a perfusion cell culture to a high cell density (i.e., at least above about 40×106 cells/N mL) and then switching to a protein production phase, wherein the cells are cultured in a fed-batch cell culture. The present invention further provides a method for clarifying a protein from a cell culture by adjusting the pH of the cell culture to below neutral pH (i.e., below a pH of 7) and settling the cell culture, such that the cell culture separates to form a supernatant layer and a cell-bed layer, wherein the protein is in the supernatant layer.

    摘要翻译： 本发明提供了一种通过将灌注细胞培养物中产生蛋白质（例如，生长期）的细胞生长至高细胞密度（即，至少高于约40×10 6个细胞/小时）来增加细胞培养物中蛋白质产生的方法， N mL），然后切换到蛋白质生产阶段，其中细胞在补料分批细胞培养物中培养。 本发明还提供了一种通过将细胞培养物的pH调节至低于中性pH（即低于pH7）来澄清来自细胞培养物的蛋白质的方法，并沉降细胞培养物，使得细胞培养物分离形成 上清液层和细胞床层，其中蛋白质在上清液层中。

    Method and apparatus for positioning a wellhead member including an overpull indicator
    8.
    发明授权
    Method and apparatus for positioning a wellhead member including an overpull indicator 有权
    用于定位包括过度指示器的井口构件的方法和装置

    公开(公告)号：US08689888B2

    公开(公告)日：2014-04-08

    申请号：US12913553

    申请日：2010-10-27

    申请人： Sibu Varghese ,  Ryan Herbel ,  Dien Nguyen ,  Javier Garcia

    发明人： Sibu Varghese ,  Ryan Herbel ,  Dien Nguyen ,  Javier Garcia

    IPC分类号： E21B33/03

    CPC分类号： E21B17/10 ,  E21B33/04

    摘要： An outer wellhead member has a bore with a first profile portion and an annular recess. A tubular inner wellhead member with a centralizer/overpull ring is lowered into the outer wellhead member. The centralizer/overpull ring is biased to expand outward to engage the bore of the outer wellhead member to center the inner wellhead member within the bore as the inner wellhead member is lowered through the bore. The recess of the outer wellhead member is adapted to receive the centralizer/overpull ring and oppose axial movement of the centralizer/overpull ring to enable an upward test pull of the inner wellhead member.

    摘要翻译： 外部井口构件具有带有第一轮廓部分和环形凹部的孔。 具有扶正器/超螺旋环的管状内部井口构件下降到外部井口构件中。 当内部井口构件通过孔下降时，对中器/超负荷环被偏压以向外膨胀以接合外部井口构件的孔，以将内部井口构件定位在孔内。 外部井口构件的凹部适于接收扶正器/超车环，并且与对置器/推杆环的轴向运动相反，以使内部井口构件能够向上测试。

    Overpull Indicator
    10.
    发明申请
    Overpull Indicator 有权
    超量指示器

    公开(公告)号：US20120103597A1

    公开(公告)日：2012-05-03

    申请号：US12913553

    申请日：2010-10-27

    申请人： Sibu Varghese ,  Ryan Herbel ,  Dien Nguyen ,  Javier Garcia

    发明人： Sibu Varghese ,  Ryan Herbel ,  Dien Nguyen ,  Javier Garcia

    IPC分类号： E21B17/10 ,  B23P17/04

    CPC分类号： E21B17/10 ,  E21B33/04

    摘要： An outer wellhead member has a bore with a first profile portion and an annular recess. A tubular inner wellhead member with a centralizer/overpull ring is lowered into the outer wellhead member. The centralizer/overpull ring is biased to expand outward to engage the bore of the outer wellhead member to center the inner wellhead member within the bore as the inner wellhead member is lowered through the bore. The recess of the outer wellhead member is adapted to receive the centralizer/overpull ring and oppose axial movement of the centralizer/overpull ring to enable an upward test pull of the inner wellhead member.

    摘要翻译： 外部井口构件具有带有第一轮廓部分和环形凹部的孔。 具有扶正器/超螺旋环的管状内部井口构件下降到外部井口构件中。 当内部井口构件通过孔下降时，对中器/超负荷环被偏压以向外膨胀以接合外部井口构件的孔，以将内部井口构件定位在孔内。 外部井口构件的凹部适于接收扶正器/超车环，并且与对置器/推杆环的轴向运动相反，以使内部井口构件能够向上测试。