公开(公告)号：US20130338479A1

公开(公告)日：2013-12-19

申请号：US13140748

申请日：2009-12-18

申请人： Brian William Pogue ,  Venkataramanan Krishnaswamy ,  Keith D. Paulsen ,  Pilar Beatriz Garcia Allende ,  Olga Maria Conde ,  José Miguel Lopez-Higuera

发明人： Brian William Pogue ,  Venkataramanan Krishnaswamy ,  Keith D. Paulsen ,  Pilar Beatriz Garcia Allende ,  Olga Maria Conde ,  José Miguel Lopez-Higuera

IPC分类号： A61B5/00 ,  A61B5/145

CPC分类号： A61B5/0068 ,  A61B5/0059 ,  A61B5/0062 ,  A61B5/0071 ,  A61B5/14546 ,  A61B5/7264 ,  A61B5/7267 ,  A61B5/742 ,  A61B2576/00 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/0218 ,  G01J3/06 ,  G01J3/10 ,  G01J3/2823 ,  G01N21/4795 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6417 ,  G01N2021/6471 ,  G01N2021/6484 ,  G01N2201/0221 ,  G01N2201/0618 ,  G01N2201/1296 ,  G06F19/3481 ,  G16H40/63

摘要： A method and apparatus is described for optically scanning a field of view, the field of view including at least part of an organ as exposed during surgery, and for identifying and classifying areas of tumor within the field of view. The apparatus obtains a spectrum at each pixel of the field of view, and classifies pixels with a kNN-type or neural network classifier previously trained on samples of tumor and organ classified by a pathologist. Embodiments use statistical parameters extracted from each pixel and neighboring pixels. Results are displayed as a color-encoded map of tissue types to the surgeon. In variations, the apparatus provides light at one or more fluorescence stimulus wavelengths and measures the fluorescence light spectrum emitted from tissue corresponding to each stimulus wavelength. The measured emitted fluorescence light spectra are further used by the classifier to identify tissue types in the field of view.

摘要翻译： 描述了用于光学扫描视野的方法和装置，视野包括在外科手术期间暴露的器官的至少一部分，以及用于识别和分类视野内的肿瘤区域。 该装置在视场的每个像素处获得光谱，并且使用先前已经由病理学家分类的肿瘤和器官样本训练的kNN型或神经网络分类器对像素进行分类。 实施例使用从每个像素和相邻像素提取的统计参数。 结果显示为外科医生的组织类型的颜色编码图。 在变型中，该装置在一个或多个荧光刺激波长处提供光，并测量从对应于每个刺激波长的组织发射的荧光光谱。 测量的发射荧光光谱被分类器进一步用于鉴定视野中的组织类型。

公开(公告)号：US09254103B2

公开(公告)日：2016-02-09

申请号：US13586558

申请日：2012-08-15

申请人： Venkataramanan Krishnaswamy ,  Brian William Pogue

发明人： Venkataramanan Krishnaswamy ,  Brian William Pogue

IPC分类号： H04N13/00 ,  A61B5/00 ,  A61B19/00 ,  G02B21/36 ,  H04N13/02 ,  A61B5/1455

CPC分类号： A61B5/0042 ,  A61B5/0071 ,  A61B5/0073 ,  A61B5/14553 ,  A61B5/7257 ,  A61B90/20 ,  A61B2090/366 ,  G02B21/365 ,  H04N13/239

摘要： An imaging system has a microscope having an objective lens and a projection device configured to project spatially modulated light in one of several preselected predetermined pattern through the objective lens and onto tissue. The system camera configured to record an image of the tissue through the microscope and objective lens as illuminated by the spatially modulated light, and an image processor having a memory with a routine for performing spatial Fourier analysis on the image of the tissue to recover spatial frequencies. The image processor also constructs a three dimensional model of the tissue, and performs fitting of at least absorbance and scattering parameters of voxels of the model to match the recovered spatial frequencies. The processor then displays tomographic slices of the three dimensional model.

摘要翻译： 成像系统具有具有物镜的显微镜和投影装置，该投影装置被配置为将经空间调制的光以几种预先选定的预定图案中的一种投影通过物镜投影到组织上。 所述系统照相机被配置为通过由空间调制光照射的显微镜和物镜记录组织的图像，以及具有存储器的图像处理器，该存储器具有用于对组织的图像进行空间傅立叶分析以恢复空间频率 。 图像处理器还构建组织的三维模型，并且对模型的体素的至少吸光度和散射参数进行拟合以匹配恢复的空间频率。 然后处理器显示三维模型的断层图像。

公开(公告)号：US20130044185A1

公开(公告)日：2013-02-21

申请号：US13586558

申请日：2012-08-15

申请人： Venkataramanan Krishnaswamy ,  Brian William Pogue

发明人： Venkataramanan Krishnaswamy ,  Brian William Pogue

IPC分类号： H04N13/00

CPC分类号： A61B5/0042 ,  A61B5/0071 ,  A61B5/0073 ,  A61B5/14553 ,  A61B5/7257 ,  A61B90/20 ,  A61B2090/366 ,  G02B21/365 ,  H04N13/239

摘要： An imaging system has a microscope having an objective lens and a projection device configured to project spatially modulated light in one of several preselected predetermined pattern through the objective lens and onto tissue. The system camera configured to record an image of the tissue through the microscope and objective lens as illuminated by the spatially modulated light, and an image processor having a memory with a routine for performing spatial Fourier analysis on the image of the tissue to recover spatial frequencies. The image processor also constructs a three dimensional model of the tissue, and performs fitting of at least absorbance and scattering parameters of voxels of the model to match the recovered spatial frequencies. The processor then displays tomographic slices of the three dimensional model.

摘要翻译： 成像系统具有具有物镜的显微镜和投影装置，该投影装置被配置为将经空间调制的光以几种预先选定的预定图案中的一种投影通过物镜投影到组织上。 所述系统照相机被配置为通过由空间调制光照射的显微镜和物镜记录组织的图像，以及具有存储器的图像处理器，该存储器具有用于对组织的图像进行空间傅立叶分析以恢复空间频率的程序 。 图像处理器还构建组织的三维模型，并且对模型的体素的至少吸光度和散射参数进行拟合以匹配恢复的空间频率。 然后处理器显示三维模型的断层图像。

公开(公告)号：US08634082B2

公开(公告)日：2014-01-21

申请号：US12665518

申请日：2008-06-20

申请人： Shudong Jiang ,  Brian William Pogue ,  Jia Wang ,  Keith D. Paulsen

发明人： Shudong Jiang ,  Brian William Pogue ,  Jia Wang ,  Keith D. Paulsen

IPC分类号： G01B9/02

CPC分类号： G01N21/4795 ,  A61B5/0073

摘要： A diffuse optical tomography system incorporating a mode-locked, tunable laser produces pulsed light that may be used to interrogate tissue with high spatial and spectral resolution. The detection signal may be heterodyne shifted to lower frequencies to allow easy and accurate measurement of phase and amplitude. Embodiments incorporating wavelength-swept, tunable, lasers and embodiments using broadband photonic fiber lasers with spectrally-sensitive detectors are described.

摘要翻译： 结合锁模的可调谐激光器的漫射光学断层扫描系统产生可用于以高空间和光谱分辨率询问组织的脉冲光。 检测信号可以是外差偏移到较低频率，以便容易且准确地测量相位和振幅。 描述了使用具有光谱敏感检测器的宽带光子激光器的波长扫描，可调谐，激光器和实施例的实施例。

公开(公告)号：US20100265493A1

公开(公告)日：2010-10-21

申请号：US12665518

申请日：2008-06-20

申请人： Shudong Jiang ,  Brian William Pogue ,  Jia Wang ,  Keith D. Paulsen

发明人： Shudong Jiang ,  Brian William Pogue ,  Jia Wang ,  Keith D. Paulsen

IPC分类号： G01N21/47 ,  G01N21/00 ,  G01J3/00

CPC分类号： G01N21/4795 ,  A61B5/0073

摘要： A diffuse optical tomography system incorporating a mode-locked, tunable laser produces pulsed light that may be used to interrogate tissue with high spatial and spectral resolution. The detection signal may be heterodyne shifted to lower frequencies to allow easy and accurate measurement of phase and amplitude. Embodiments incorporating wavelength-swept, tunable, lasers and embodiments using broadband photonic fiber lasers with spectrally-sensitive detectors are described.

摘要翻译： 结合锁模的可调谐激光器的漫射光学断层扫描系统产生可用于以高空间和光谱分辨率询问组织的脉冲光。 检测信号可以是外差偏移到较低频率，以便容易且准确地测量相位和振幅。 描述了使用具有光谱敏感检测器的宽带光子激光器的波长扫描，可调谐，激光器和实施例的实施例。

公开(公告)号：US08000775B2

公开(公告)日：2011-08-16

申请号：US12088845

申请日：2006-04-27

申请人： Brian William Pogue ,  Daqing Piao ,  Keith D. Paulsen ,  Shudong Jiang ,  Hamid Dehghani ,  Heng Xu ,  Roger Springett ,  Subhadra Srinivasan

发明人： Brian William Pogue ,  Daqing Piao ,  Keith D. Paulsen ,  Shudong Jiang ,  Hamid Dehghani ,  Heng Xu ,  Roger Springett ,  Subhadra Srinivasan

IPC分类号： A61B6/00

CPC分类号： A61B5/0091 ,  A61B5/0073 ,  A61B5/14551 ,  A61B5/4312 ,  A61B5/7239

摘要： Optical tomography systems that provide light of multiple distinct wavelengths from a plurality of sources are described. The systems direct light into mammalian tissue, and light from the mammalian tissue is collected at a plurality of reception points. Collected light from each reception point is separated according to its wavelength, and received by a photodetector to produce path attenuation signals representing attenuation along paths between the source locations and the reception points. An image construction system generates a tomographic image of the mammalian tissue from the path attenuation signals. One embodiment of an optical imaging system includes an optical coherence tomography-near infrared probe. The systems and methods may utilize a spectral derivative approach that provides insensitivity to the boundary and boundary artifacts in the signal, thereby improving the quality of the reconstructed images.

摘要翻译： 描述了从多个源提供多个不同波长的光的光学层析成像系统。 系统将光引入哺乳动物组织，并且在多个接收点收集来自哺乳动物组织的光。 来自每个接收点的收集的光根据其波长被分离，并由光电检测器接收以产生表示沿着源位置和接收点之间的路径衰减的路径衰减信号。 图像构造系统从路径衰减信号生成哺乳动物组织的断层图像。 光学成像系统的一个实施例包括光学相干断层摄影近红外探测器。 系统和方法可以利用对信号中的边界和边界伪像提供不敏感性的光谱导数方法，从而提高重建图像的质量。

公开(公告)号：US20090247847A1

公开(公告)日：2009-10-01

申请号：US12088845

申请日：2006-04-27

申请人： Brian William Pogue ,  Daqing Piao ,  Keith D. Paulsen ,  Shudong Jiang ,  Hamid Dehghani ,  Heng Xu ,  Roger Springett ,  Subhadra Srinivasan

发明人： Brian William Pogue ,  Daqing Piao ,  Keith D. Paulsen ,  Shudong Jiang ,  Hamid Dehghani ,  Heng Xu ,  Roger Springett ,  Subhadra Srinivasan

IPC分类号： A61B6/03 ,  A61B5/1455

CPC分类号： A61B5/0091 ,  A61B5/0073 ,  A61B5/14551 ,  A61B5/4312 ,  A61B5/7239

摘要： Optical tomography systems that provide light of multiple distinct wavelengths from a plurality of sources are described. The systems direct light into mammalian tissue, and light from the mammalian tissue is collected at a plurality of reception points. Collected light from each reception point is separated according to its wavelength, and received by a photodetector to produce path attenuation signals representing attenuation along paths between the source locations and the reception points. An image construction system generates a tomographic image of the mammalian tissue from the path attenuation signals. One embodiment of an optical imaging system includes an optical coherence tomography-near infrared probe. The systems and methods may utilize a spectral derivative approach that provides insensitivity to the boundary and boundary artifacts in the signal, thereby improving the quality of the reconstructed images.

摘要翻译： 描述了从多个源提供多个不同波长的光的光学层析成像系统。 系统将光引入哺乳动物组织，并且在多个接收点收集来自哺乳动物组织的光。 来自每个接收点的收集的光根据其波长被分离，并由光电检测器接收以产生表示沿着源位置和接收点之间的路径衰减的路径衰减信号。 图像构造系统从路径衰减信号生成哺乳动物组织的断层图像。 光学成像系统的一个实施例包括光学相干断层摄影近红外探测器。 系统和方法可以利用对信号中的边界和边界伪像提供不敏感性的光谱导数方法，从而提高重建图像的质量。

公开(公告)号：US10201718B2

公开(公告)日：2019-02-12

申请号：US14118825

申请日：2012-05-18

申请人： Brian William Pogue ,  David Joseph Gladstone ,  Scott Christian Davis ,  Johan Jakob Axelsoon ,  Adam Kenneth Glaser ,  Rongxiao Zhang

发明人： Brian William Pogue ,  David Joseph Gladstone ,  Scott Christian Davis ,  Johan Jakob Axelsoon ,  Adam Kenneth Glaser ,  Rongxiao Zhang

IPC分类号： A61B5/05 ,  A61B5/00 ,  A61N5/10 ,  A61B5/1455 ,  G01T1/22

摘要： A system for providing monitored radiation therapy has a high energy radiation source, apparatus for excluding uncontrolled ambient light, and apparatus for collecting light emitted from a subject. The system has apparatus for spectrally analyzing the collected light, and a processor for determining oxygenation or other metabolic function of tissue within the subject from spectral analysis of the collected light. The system monitors radiation therapy by providing a beam of high energy radiation; collecting Cherenkov and/or photoluminescent light from the subject, the light generated along the beam; spectrally analyzing the light; and determining oxygenation or metabolic function of tissue from the spectral analysis. Beam profile of the system is calibrated by imaging from multiple angles Cherenkov and/or photoluminescent light emitted by a phantom placed in the beam in lieu of a subject, captured images are analyzed to determine beam profile.

公开(公告)号：US07962198B2

公开(公告)日：2011-06-14

申请号：US11115865

申请日：2005-04-27

申请人： Brian William Pogue ,  Daqing Piao

发明人： Brian William Pogue ,  Daqing Piao

IPC分类号： A61B6/00

CPC分类号： A61B5/0091 ,  A61B5/0073 ,  A61B5/14551 ,  A61B5/4312 ,  A61B5/7239

摘要： An optical tomography system has a group of lasers for generating light of multiple distinct wavelengths within a first wavelength band. Light from these lasers is directed into mammalian tissue at laser-specific locations on the tissue, and light from the mammalian tissue is collected at a plurality of reception points. Collected light from each reception point is separated according to its wavelength, and received by a photodetector to produce path attenuation signals representing attenuation along paths between the laser-specific locations on the tissue and the reception points. Image construction apparatus generates a tomographic image of heme concentrations in the mammalian tissue from the path attenuation signals. In an alternative embodiment, there is a second group of lasers operating in a second wavelength band, and the image construction apparatus can generate an image of heme oxygenation In the mammalian tissue.

摘要翻译： 光学断层摄影系统具有用于在第一波长带内产生多个不同波长的光的一组激光器。 来自这些激光器的光被引导到组织上的激光特定位置处的哺乳动物组织中，并且来自哺乳动物组织的光在多个接收点被收集。 来自每个接收点的收集的光根据其波长被分离，并由光电检测器接收，以产生表示沿着组织上的激光器特定位置和接收点之间的路径衰减的路径衰减信号。 图像构造装置从路径衰减信号生成哺乳动物组织中血红素浓度的断层图像。 在替代实施例中，存在在第二波长带中操作的第二组激光器，并且图像构建装置可以在哺乳动物组织中产生血红素氧合图像。

公开(公告)号：US06377842B1

公开(公告)日：2002-04-23

申请号：US09379001

申请日：1999-08-23

申请人： Brian William Pogue ,  Gregory Charles Burke

发明人： Brian William Pogue ,  Gregory Charles Burke

IPC分类号： A61B500

CPC分类号： A61B5/0059 ,  A61B5/7242 ,  A61N5/0601 ,  A61N5/062

摘要： A fiber optic based probe has been designed to sample the fluorescence or phosphorescence signal from animal or human tissues, such that the light intensity is not multiply scattered. This type of measurement allows a linear detection of the concentration of the luminescent compound non-invasively from the tissue. The basic principle of the fiber probe is to use fiber optics which are smaller in diameter that the average scattering length of the tissue. In order to increase the detected signal to a stronger level, multiple fibers are used by spacing them out on the surface of the tissue so that each fiber samples an isolated section of tissue. Each fiber delivers the excitation light to the tissue, and receives the emission light from the tissue. All fibers are coupled into the same detector to integrate the overall signal. Sampling of the scattered excitation signal intensity is also done to correct for changes in the scattering coefficient between tissues. This fiber bundle can be used to quantify the concentration of fluorescence or phosphorescent chemicals in vivo non-invasively. One application of this device is as a dosimetry tool, to monitor photosensitizer drug concentration within tissue for photodynamic therapy.

摘要翻译： 已经设计了一种基于光纤的探针来对动物或人体组织的荧光或磷光信号进行采样，使得光强度不会被分散。 这种类型的测量允许从组织非侵入地线性检测发光化合物的浓度。 纤维探针的基本原理是使用直径较小的纤维，即组织的平均散射长度。 为了将检测到的信号增加到更强的水平，通过在组织的表面上间隔多个纤维来使用多个纤维，使得每个纤维对组织的隔离部分进行采样。 每个纤维将激发光传递到组织，并接收来自组织的发射光。 所有光纤耦合到相同的检测器中以整合信号。 散射激发信号强度的采样也用于校正组织之间散射系数的变化。 该纤维束可用于非侵入性地定量体内荧光或磷光化学品的浓度。 该装置的一个应用是作为剂量测定工具，用于监测光动力疗法组织内的光敏剂药物浓度。