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    RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A 5-HYDROXYPENT-3-ENOATE INTERMEDIATE
    8.
    发明申请
    RECOMBINANT HOST CELLS AND PROCESSES FOR PRODUCING 1,3-BUTADIENE THROUGH A 5-HYDROXYPENT-3-ENOATE INTERMEDIATE 审中-公开
    通过5-羟基-3-烯酸中间体生产1,3-丁二烯的重组宿主细胞和方法

    公开(公告)号：US20150017698A1

    公开(公告)日：2015-01-15

    申请号：US14381137

    申请日：2013-02-26

    申请人： Codexis, Inc.

    发明人： Gregory A. Cope ,  Louis Clark

    IPC分类号： C12P5/02 ,  C12N15/76 ,  C12N15/81 ,  C12N15/70 ,  C12N15/75

    CPC分类号： C12P5/026 ,  C12N15/52 ,  C12N15/70 ,  C12N15/75 ,  C12N15/76 ,  C12N15/81 ,  C12N15/815 ,  Y02E50/343

    摘要： The present disclosure relates to recombinant host cells comprising one or more recombinant polynucleotides encoding enzymes in select pathways that provide the ability to use the cells to produce 1,3-butadiene. The present disclosure also provides methods of manufacturing the recombinant host cells, and methods for the use of the cells to produce 1,3-butadiene. The methods utilize recombinant host cells that comprise an engineered pathway of enzymes that provides for the conversion of naturally occurring intermediate crotonyl-CoA (or -ACP) to 1,3-butadiene through enzyme catalyzed steps involving the reduction of glutaconyl-CoA (or -ACP) to form the intermediate 5-hydroxypent-3-enoate. The disclosure provides alternative engineered pathway involving either decarboxylation of 5-hydroxypent-3-enoate directly to 1,3-butadiene, or phosphorylation of 5-hydroxypent-3-enoate followed by a phosphate elimination step catalyzed by a decarboxylase to produce 1,3-butadiene.

    摘要翻译： 本公开涉及重组宿主细胞，其包含一种或多种编码选择途径中的酶的重组多核苷酸，其提供使用细胞产生1,3-丁二烯的能力。 本公开还提供了制备重组宿主细胞的方法，以及使用细胞产生1,3-丁二烯的方法。 该方法利用重组宿主细胞，其包含酶的工程化途径，其通过涉及还原戊酰辅酶A（或 - ）的酶催化步骤来提供将天然存在的中间体巴豆酰辅酶A（或-ACP）转化为1,3-丁二烯， ACP）以形成中间体5-羟基-3-烯酸酯。 本公开提供了涉及5-羟基-3-丁烯酸直接转化为1,3-丁二烯或5-羟基-3-戊酸磷酸化的替代工程化途径，接着是由脱羧酶催化的磷酸盐消除步骤产生1,3 丁二烯。