公开(公告)号：US09476856B2

公开(公告)日：2016-10-25

申请号：US13890715

申请日：2013-05-09

Applicant: Advanced Liquid Logic Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N33/543 ,  G01N27/72 ,  G01N27/74

CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。

公开(公告)号：US20160114320A1

公开(公告)日：2016-04-28

申请号：US14935823

申请日：2015-11-09

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

公开(公告)号：US20220155251A1

公开(公告)日：2022-05-19

申请号：US17649842

申请日：2022-02-03

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vamsee K. Pamula ,  Vijay Srinivasan ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/26 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  B01F33/302 ,  B01F33/3031 ,  C12Q1/686 ,  C12Q1/6869 ,  G01N27/447 ,  G01N27/327 ,  G01N27/416

Abstract: The present invention relates to droplet-based surface modification and washing. According to one embodiment, a method of splitting a droplet is provided, the method including providing a droplet microactuator including a droplet including one or more beads and immobilizing at least one of the one or more beads. The method further includes conducting one or more droplet operations to divide the droplet to yield a set of droplets including a droplet including the one or more immobilized beads and a droplet substantially lacking the one or more immobilized beads.

公开(公告)号：US20150336098A1

公开(公告)日：2015-11-26

申请号：US14817834

申请日：2015-08-04

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00

CPC classification number: G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

Abstract translation: 公开了用于操纵液滴的装置和方法。 在一个实施例中，提供了一种用于操纵液滴的装置，该装置包括一个基板，设置在该基板上的多个电极阵列，其中每个阵列中的对应电极连接到公共电信号，以及首先设置在基板上的电介质层 侧表面并图案化以覆盖电极。

公开(公告)号：US09180450B2

公开(公告)日：2015-11-10

申请号：US14446733

申请日：2014-07-30

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: G01N27/453 ,  B01L3/00 ,  B01F11/00 ,  B01F13/00 ,  G02B26/00 ,  C25B9/00 ,  B41J2/14

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US20150314293A1

公开(公告)日：2015-11-05

申请号：US14719731

申请日：2015-05-22

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: B01L3/00 ,  G01N27/447

CPC classification number: G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

Abstract: A method of providing a droplet in contact with a magnetically responsive bead and having a reduced quantity of a substance. The method generally includes the steps of (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged for conducting droplet operations on a surface; (ii) a starting droplet comprising: (1) one or more magnetically responsive beads; (2) a starting quantity of the substance; and (3) a starting volume; (b) magnetically immobilizing the one or more magnetically responsive beads at a location which is at a distance from a target droplet splitting zone; (c) conducting one or more droplet operations comprising droplet dividing operations selected to divide the combined droplet to yield a set of droplets comprising: (i) a droplet comprising substantially all of the one or more magnetically responsive beads and having a decreased quantity of the substance relative to the starting concentration; and (ii) a droplet substantially lacking the magnetically responsive beads.

Abstract translation: 提供与磁响应珠接触并具有减少的物质量的液滴的方法。 该方法通常包括以下步骤：（a）提供液滴致动器，其包括：（i）包括布置用于在表面上进行液滴操作的电极的基板; （ii）起始液滴，包括：（1）一个或多个磁响应珠; （2）物质的起始量; 和（3）起始体积; （b）将一个或多个磁响应珠磁性地固定在距离目标液滴分裂区一定距离的位置; （c）进行一个或多个液滴操作，其包括液滴分割操作，所述液滴分割操作被选择以分开所述组合的液滴以产生一组液滴，所述液滴包括：（i）包含基本上所有所述一个或多个磁响应珠粒并且具有减少量 物质相对于起始浓度; 和（ii）基本上不含磁响应珠的液滴。

公开(公告)号：US20150174577A1

公开(公告)日：2015-06-25

申请号：US14639612

申请日：2015-03-05

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Philip Y. Paik ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: B01L3/00 ,  B03C5/02 ,  B81B1/00

CPC classification number: B01L3/502792 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L7/54 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/0448 ,  B03C5/02 ,  B81B1/00 ,  G01N27/447 ,  G01N2035/1046

Abstract: The present invention relates to filler fluids for droplet operations. According to one embodiment of this aspect, a droplet microactuator is provided and includes: (a) a first substrate comprising electrodes configured for conducting droplet operations on a surface of the substrate; (b) a second substrate spaced from the surface of the substrate by a distance sufficient to define an interior volume between the first substrate and second substrate, wherein the distance is sufficient to contain a droplet disposed in the space on the first substrate; and (c) a droplet arranged in the interior volume and arranged with respect to the electrodes in a manner which permits droplet operations to be effected on the droplet using the electrodes.

Abstract translation: 本发明涉及用于液滴操作的填充流体。 根据该方面的一个实施例，提供了液滴微致动器，其包括：（a）第一基板，其包括被配置用于在所述基板的表面上进行液滴操作的电极; （b）第二衬底，其与衬底的表面间隔足以限定第一衬底和第二衬底之间的内部空间的距离，其中所述距离足以容纳设置在第一衬底上的空间中的液滴; 和（c）布置在内部体积中并且以允许使用电极对液滴进行液滴操作的方式相对于电极布置的液滴。

公开(公告)号：US20150060284A1

公开(公告)日：2015-03-05

申请号：US14538322

申请日：2014-11-11

Applicant: DUKE UNIVERSITY

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B. Fair

IPC: G01N27/447 ,  B01L3/00 ,  B01L7/00 ,  C12Q1/68

CPC classification number: G01N27/44769 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502707 ,  B01L3/502792 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/1827 ,  B01L2400/0415 ,  B01L2400/0424 ,  B81B3/0021 ,  B81B2201/058 ,  B81C1/00119 ,  C12Q1/6846 ,  G01N27/447 ,  G01N27/44791 ,  G05D7/0694 ,  H01L21/6715 ,  Y10T436/2575

Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.

公开(公告)号：US08871071B2

公开(公告)日：2014-10-28

申请号：US13759416

申请日：2013-02-05

Applicant: Duke University

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Richard B Fair

IPC: G01N27/447 ,  G01N27/453 ,  C25B9/00 ,  B01L3/00 ,  B01F13/00 ,  B01F11/00 ,  G02B26/00 ,  B41J2/14

CPC classification number: B01L3/50273 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01F13/0084 ,  B01L3/00 ,  B01L3/5027 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2200/0605 ,  B01L2200/10 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0427 ,  B41J2002/14395 ,  C25B9/00 ,  G01N27/44791 ,  G02B26/005

Abstract: Methods are provided for manipulating droplets. The methods include providing the droplet on a surface comprising an array of electrodes and a substantially co-planer array of reference elements, wherein the droplet is disposed on a first one of the electrodes, and the droplet at least partially overlaps a second one of the electrodes and an intervening one of the reference elements disposed between the first and second electrodes. The methods further include activating the first and second electrodes to spread at least a portion of the droplet across the second electrode and deactivating the first electrode to move the droplet from the first electrode to the second electrode.

Abstract translation: 提供了用于操纵液滴的方法。 所述方法包括在包括电极阵列和基本上共平面的参考元件阵列的表面上提供液滴，其中液滴设置在电极的第一个上，并且液滴至少部分地与第二个 电极和设置在第一和第二电极之间的参考元件中的插入件之一。 所述方法还包括激活第一和第二电极以将液滴的至少一部分扩散穿过第二电极，并且去激活第一电极以使液滴从第一电极移动到第二电极。

公开(公告)号：US12181467B2

公开(公告)日：2024-12-31

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  C12M1/12 ,  G01N27/447 ,  G01N35/00 ,  G01N35/10

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.