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    Screening test drugs to identify their effects on cell membrane voltage-gated ion channel
    5.
    发明授权
    Screening test drugs to identify their effects on cell membrane voltage-gated ion channel 有权
    筛选试验药物，以鉴别其对细胞膜电压门控离子通道的影响

    公开(公告)号：US09274099B2

    公开(公告)日：2016-03-01

    申请号：US12187927

    申请日：2008-08-07

    申请人： Karl Deisseroth ,  Feng Zhang ,  Viviana Gradinaru ,  M. Bret Schneider

    发明人： Karl Deisseroth ,  Feng Zhang ,  Viviana Gradinaru ,  M. Bret Schneider

    IPC分类号： A61N5/06 ,  G01N33/50 ,  C40B60/12 ,  G01N33/68

    CPC分类号： G01N33/502 ,  A61N5/0622 ,  B01J2219/00315 ,  B01J2219/00495 ,  B01J2219/00702 ,  C40B60/12 ,  G01N33/5008 ,  G01N33/6872

    摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.

    摘要翻译： 实现了各种应用，系统，方法和结构，用于与离子通道调制器的筛选相结合。 与一个这样的系统一致，筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关，电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。

    CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS
    7.
    发明申请
    CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS 有权
    细胞线，系统和方法用于离子通道调制器的光学筛选

    公开(公告)号：US20090099038A1

    公开(公告)日：2009-04-16

    申请号：US12187927

    申请日：2008-08-07

    申请人： Karl Deisseroth ,  Feng Zhang ,  Viviana Gradinaru ,  M. Bret Schneider

    发明人： Karl Deisseroth ,  Feng Zhang ,  Viviana Gradinaru ,  M. Bret Schneider

    IPC分类号： C40B30/10 ,  C12N5/06 ,  C12M1/00 ,  C40B60/12 ,  C12Q1/02

    CPC分类号： G01N33/502 ,  A61N5/0622 ,  B01J2219/00315 ,  B01J2219/00495 ,  B01J2219/00702 ,  C40B60/12 ,  G01N33/5008 ,  G01N33/6872

    摘要： A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor.

    摘要翻译： 实现了各种应用，系统，方法和结构，用于与离子通道调制器的筛选相结合。 与一个这样的系统一致，筛选候选药物以鉴定其对细胞膜离子通道和泵的影响。 该系统包括筛选具有光响应性膜离子开关，电压门控离子开关和荧光产生电压传感器的电池。 化学品输送装置引入待筛选的候选药物。 光学传递装置激活光响应离子开关。 光学传感器监测由电压传感器产生的荧光。 处理器处理从光学传感器接收的数据。 存储器存储从光学传感器接收的数据。

    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS
    8.
    发明申请
    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS 有权
    用于目标细胞光刺激的系统，方法和组合物

    公开(公告)号：US20110301529A1

    公开(公告)日：2011-12-08

    申请号：US13208419

    申请日：2011-08-12

    申请人： Feng Zhang ,  Karl Deisseroth ,  Viviana Gradinaru

    发明人： Feng Zhang ,  Karl Deisseroth ,  Viviana Gradinaru

    IPC分类号： A61M37/00 ,  C12N13/00

    CPC分类号： A61N5/062 ,  A01K67/0333 ,  A01K2217/052 ,  A01K2227/703 ,  A01K2267/0356 ,  A61K38/00 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0083 ,  A61N5/0613 ,  A61N5/0622 ,  C07K14/00 ,  C07K14/195 ,  C07K14/215 ,  C12N13/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16643 ,  C12N2740/16043 ,  C12N2740/16071 ,  C12N2750/14143 ,  C12N2830/002

    摘要： Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).

    摘要翻译： 使用光，例如体内或体外，使用各种方法和装置来实施靶细胞的刺激。 一个实例涉及用于递送光激活的基于NpHR的分子的载体，其包含编码基于光激活的基于NpHR的分子和启动子的核酸序列。 分子的高表达表现出小于约75％的毒性水平，或者使用至少两种基于NpHR的分子变体表达光激活的NpHR基蛋白。 每个变体的特征在于可用于表达基于光激活的基于NpHR的分子，其通过产生抑制电流来抑制神经元的去极化来响应光。 其他方面和实施方案涉及用于离子泵的物质和分子的组合物，或用于控制细胞中的抑制性电流（例如在体内和体外环境中）的系统，方法，试剂盒，组合物。

    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS
    9.
    发明申请
    SYSTEMS, METHODS AND COMPOSITIONS FOR OPTICAL STIMULATION OF TARGET CELLS 审中-公开
    用于目标细胞光刺激的系统，方法和组合物

    公开(公告)号：US20090093403A1

    公开(公告)日：2009-04-09

    申请号：US12041628

    申请日：2008-03-03

    申请人： Feng Zhang ,  Karl Deisseroth ,  Viviana Gradinaru

    发明人： Feng Zhang ,  Karl Deisseroth ,  Viviana Gradinaru

    IPC分类号： A61K38/17 ,  C07H21/04 ,  C07K14/435 ,  A61P25/00 ,  C12N15/63

    CPC分类号： A61N5/062 ,  A01K67/0333 ,  A01K2217/052 ,  A01K2227/703 ,  A01K2267/0356 ,  A61K38/00 ,  A61K48/005 ,  A61K48/0058 ,  A61K48/0083 ,  A61N5/0613 ,  A61N5/0622 ,  C07K14/00 ,  C07K14/195 ,  C07K14/215 ,  C12N13/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/16643 ,  C12N2740/16043 ,  C12N2740/16071 ,  C12N2750/14143 ,  C12N2830/002

    摘要： Stimulation of target cells using light, e.g., in vivo or in vitro, is implemented using a variety of methods and devices. One example involves a vector for delivering a light-activated NpHR-based molecule comprising a nucleic acid sequence that codes for light-activated NpHR-based molecule and a promoter. Either a high expression of the molecule manifests a toxicity level that is less than about 75%, or the light-activated NpHR-based proteins are expressed using at least two NpHR-based molecular variants. Each of the variants characterized in being useful for expressing a light-activated NpHR-based molecule that responds to light by producing an inhibitory current to dissuade depolarization of the neuron. Other aspects and embodiments are directed to systems, methods, kits, compositions of matter and molecules for ion pumps or for controlling inhibitory currents in a cell (e.g., in in vivo and in vitro environments).

    摘要翻译： 使用光，例如体内或体外，使用各种方法和装置来实施靶细胞的刺激。 一个实例涉及用于递送光激活的基于NpHR的分子的载体，其包含编码基于光激活的基于NpHR的分子和启动子的核酸序列。 分子的高表达表现出小于约75％的毒性水平，或者使用至少两种基于NpHR的分子变体表达光激活的NpHR基蛋白。 每个变体的特征在于可用于表达基于光激活的基于NpHR的分子，其通过产生抑制电流来抑制神经元的去极化来响应光。 其他方面和实施方案涉及用于离子泵的物质和分子的组合物，或用于控制细胞中的抑制性电流（例如在体内和体外环境中）的系统，方法，试剂盒，组合物。

    Functional Targeted Brain Endoskeletonization
    10.
    发明申请
    Functional Targeted Brain Endoskeletonization 审中-公开
    功能性目标脑内骨骼化

    公开(公告)号：US20140030192A1

    公开(公告)日：2014-01-30

    申请号：US13980842

    申请日：2012-01-26

    申请人： Karl A. Deisseroth ,  Viviana Gradinaru

    发明人： Karl A. Deisseroth ,  Viviana Gradinaru

    IPC分类号： A61K48/00

    CPC分类号： G01N1/30 ,  A61K38/1748 ,  A61K48/00 ,  G01N1/36

    摘要： Compositions and methods are provided for TEMPEST (Target-Element Modification by Physical and Enduring Structural Transmutation), a method for creating durable structures in vivo in a cell-type and/or circuit specific manner via the use of insoluble polymers. TEMPEST provides a way to functionally remove cells while preserving their “shadow” for easy post-experiment detection and classification. The method of the invention are of particular interest for modifying neurons, which may be central nervous system or peripheral nervous system cells, however the approach may be applied to other cellular systems as well, either in culture system models or in animals.

    摘要翻译： 提供了TEMPEST（物理和持续结构变异的靶元素修饰）的组合物和方法，该方法通过使用不溶性聚合物以细胞型和/或电路特异性方式在体内产生耐久结构。 TEMPEST提供了一种在功能上移除细胞的方法，同时保留其“阴影”，便于实验后检测和分类。 本发明的方法对于修饰可能是中枢神经系统或周围神经系统细胞的神经元是特别有意义的，然而该方法也可以在培养系统模型或动物中应用于其他细胞系统。