Process for the preparation of defective mutants of microorganisms
    2.
    发明授权
    Process for the preparation of defective mutants of microorganisms 失效
    制备微生物缺陷突变体的方法

    公开(公告)号:US4362815A

    公开(公告)日:1982-12-07

    申请号:US128223

    申请日:1980-03-07

    摘要: For the production of 17-C-steroid-.alpha.-propionic acid compounds by microbial side chain degradation on 17-C-side chain steroid substrates wild strain microorganisms selected in predetermined manner are subjected to a multi-stage mutation and selection. The resultant mutant strains are capable of supplying the desired compounds, e.g. .DELTA.-4 BNC and/or .DELTA.-1,4 BNC in high yields even in the absence of inhibitors arresting the growth and/or degradation of the steroid ring. Wild strains and mutants particularly suitable, and their choice, production and use, are described.

    摘要翻译: 为了通过在17-C侧链类固醇底物上的微生物侧链降解生产17-C-类固醇-α-丙酸化合物,以预定方式选择的野生菌株微生物进行多阶段突变和选择。 所得到的突变菌株能够提供所需化合物,例如 即使在没有阻止类固醇环的生长和/或降解的抑制剂的情况下,也能以高产率产生DELTA-4BNC和/或DELTA-1,4BNC。 描述了特别适合的野生株和突变体及其选择,生产和使用。

    3-Oxo-pregna-1,14,17-trien-20-carboxylates and process
    4.
    发明授权
    3-Oxo-pregna-1,14,17-trien-20-carboxylates and process 失效
    3-氧代 - 前 - 1,14,17-三烯-20-羧酸酯和方法

    公开(公告)号:US4333880A

    公开(公告)日:1982-06-08

    申请号:US228177

    申请日:1981-01-26

    IPC分类号: C12P33/16 C07J9/00 C07J13/00

    CPC分类号: C07J13/007 C07J9/005

    摘要: A .DELTA..sup.1,4,17 -BNC compound having the formula: ##STR1## wherein X is a substituent selected from the group consisting of OH and OCH.sub.3 ; as well as its process of production by microbiological side-chain splitting of a 17-C-side-chain steroid with a defect mutant microorganism which gives a steroid with a 17-C-.alpha.-propionic acid substituent in the absence of inhibitors inhibiting either the cleavage of the steroid ring or microorganism growth, where the defect mutant microorganisms are grown under a reduced air supply whereby an enrichment of .DELTA..sup.1,4,17 -BNC occurs.

    摘要翻译: 具有下式的DELTA 1,4,17-BNC化合物:其中X是选自OH和OCH 3的取代基; 以及其通过17-C-侧链类固醇与缺失突变体微生物的微生物侧链裂解的生产过程,所述缺陷突变体微生物在不存在抑制剂的情况下给予具有17-C-α-丙酸取代基的类固醇 类固醇环或微生物生长的切割,其中缺陷突变体微生物在减少的空气供应下生长,从而发生DELTA 1,4,17-BNC的富集。

    Stirling machine
    10.
    发明授权
    Stirling machine 有权
    斯特林机

    公开(公告)号:US09109533B2

    公开(公告)日:2015-08-18

    申请号:US13636614

    申请日:2011-03-29

    摘要: This Stirling machine comprises a transfer piston (6, 6a) and a moving part (14) of a generator or of an electric motor, the transfer piston (6, 6a) periodically displacing a working gas between an expansion chamber (VE) and a compression chamber (Vc) which chambers are respectively associated with two working faces of the transfer piston (6, 6a) of which the cross-sectional area ratio ac/aE is >0.35 so that its displacement along an axis X oriented towards the expansion volume (VE) generates an in-phase working gas pressure component Px that opposes the displacement of the piston (6, 6a), so that all of the mechanical energy produced is transmitted to the moving part (14). This machine comprises a resonant second piston (10) coupled to the transfer piston (6, 6a) by a quantity of energy that is proportional to the pressure component Px.

    摘要翻译: 该斯特林机器包括发电机或电动机的转移活塞(6,6a)和运动部分(14),所述转移活塞(6,6a)周期性地使工作气体在膨胀室(VE)和 压缩室(Vc),其分别与传送活塞(6,6a)的两个工作面相关联,其横截面积比ac / aE大于0.35,使得其沿着朝向膨胀体积的轴线X的位移 (VE)产生与活塞(6,6a)的位移相对的同相工作气体压力分量Px,使得产生的所有机械能传递到运动部件(14)。 该机器包括通过与压力分量Px成比例的一定量的能量耦合到传送活塞(6,6a)的共振第二活塞(10)。