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公开(公告)号:US4320195A
公开(公告)日:1982-03-16
申请号:US29415
申请日:1979-04-12
申请人: Frank Hill , Wolfgang Preuss , Joachim Schindler , Rolf Schmid , Alfred Struve
发明人: Frank Hill , Wolfgang Preuss , Joachim Schindler , Rolf Schmid , Alfred Struve
IPC分类号: C12N1/20 , C07J9/00 , C12N1/00 , C12N1/02 , C12N15/00 , C12N15/01 , C12P33/00 , C12P33/16 , C12R1/025 , C12R1/06 , C12R1/07 , C12R1/13 , C12R1/15 , C12R1/20 , C12R1/32 , C12R1/365 , C12R1/425 , C12R1/465
CPC分类号: C07J9/005 , C12N15/01 , C12P33/005 , Y10S435/843
摘要: A process for the production of useful steroids obtained by side chain degradation of sterol source materials through the use of microorganisms is described.
摘要翻译: 描述了通过使用微生物通过侧链降解固醇源材料获得的有用类固醇的生产方法。
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公开(公告)号:US4362815A
公开(公告)日:1982-12-07
申请号:US128223
申请日:1980-03-07
CPC分类号: C12N15/01 , C07J9/005 , C12P33/005
摘要: For the production of 17-C-steroid-.alpha.-propionic acid compounds by microbial side chain degradation on 17-C-side chain steroid substrates wild strain microorganisms selected in predetermined manner are subjected to a multi-stage mutation and selection. The resultant mutant strains are capable of supplying the desired compounds, e.g. .DELTA.-4 BNC and/or .DELTA.-1,4 BNC in high yields even in the absence of inhibitors arresting the growth and/or degradation of the steroid ring. Wild strains and mutants particularly suitable, and their choice, production and use, are described.
摘要翻译: 为了通过在17-C侧链类固醇底物上的微生物侧链降解生产17-C-类固醇-α-丙酸化合物,以预定方式选择的野生菌株微生物进行多阶段突变和选择。 所得到的突变菌株能够提供所需化合物,例如 即使在没有阻止类固醇环的生长和/或降解的抑制剂的情况下,也能以高产率产生DELTA-4BNC和/或DELTA-1,4BNC。 描述了特别适合的野生株和突变体及其选择,生产和使用。
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公开(公告)号:US4432905A
公开(公告)日:1984-02-21
申请号:US391841
申请日:1982-06-24
申请人: Michael Bahn , Wolfgang Preuss , Rolf Schmid , Rudiger Wagner
发明人: Michael Bahn , Wolfgang Preuss , Rolf Schmid , Rudiger Wagner
CPC分类号: C12P33/08 , C07J13/007 , C07J41/005 , C07J41/0088 , C07J7/0005 , C07J7/002 , C07J7/007 , C07J9/005
摘要: The degradation of steroid-C22-carboxylic acids to the C20-carbonyl steroids is carried out in simplified form and preferably without isolating intermediate reaction products by using 17(20)-steroid-22-carboxylic acids as starting material and directly transforming them into the 20-carbonyl compound through their respective acid halides by the Curtius degradation or by carboxy-inversion degradation. The acetyl substituent thus formed in C17 of the steroid compounds is present in the pharmacologically required configuration.
摘要翻译: 类固醇-C22-羧酸对C20-羰基类固醇的降解以简化的形式进行,优选不用分离中间体反应产物,通过使用17(20) - 类固醇22-羧酸作为起始原料并将其直接转化为 20-羰基化合物通过它们各自的酰卤通过Curtius降解或羧基反转降解。 因此在类固醇化合物的C17中形成的乙酰基取代基以药理学上需要的形式存在。
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公开(公告)号:US4333880A
公开(公告)日:1982-06-08
申请号:US228177
申请日:1981-01-26
申请人: Wolfgang Preuss , Michael Bahn , Rolf Schmid , Rudiger Wagner
发明人: Wolfgang Preuss , Michael Bahn , Rolf Schmid , Rudiger Wagner
CPC分类号: C07J13/007 , C07J9/005
摘要: A .DELTA..sup.1,4,17 -BNC compound having the formula: ##STR1## wherein X is a substituent selected from the group consisting of OH and OCH.sub.3 ; as well as its process of production by microbiological side-chain splitting of a 17-C-side-chain steroid with a defect mutant microorganism which gives a steroid with a 17-C-.alpha.-propionic acid substituent in the absence of inhibitors inhibiting either the cleavage of the steroid ring or microorganism growth, where the defect mutant microorganisms are grown under a reduced air supply whereby an enrichment of .DELTA..sup.1,4,17 -BNC occurs.
摘要翻译: 具有下式的DELTA 1,4,17-BNC化合物:其中X是选自OH和OCH 3的取代基; 以及其通过17-C-侧链类固醇与缺失突变体微生物的微生物侧链裂解的生产过程,所述缺陷突变体微生物在不存在抑制剂的情况下给予具有17-C-α-丙酸取代基的类固醇 类固醇环或微生物生长的切割,其中缺陷突变体微生物在减少的空气供应下生长,从而发生DELTA 1,4,17-BNC的富集。
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公开(公告)号:US4518697A
公开(公告)日:1985-05-21
申请号:US449415
申请日:1982-12-13
CPC分类号: C12R1/685 , A23K20/189 , C12N9/62 , Y10S435/917
摘要: Process for the preparation of acid stable protease having a broad activity spectrum in high yields from mutants of the fungus Aspergillus, and the mutants used therein.
摘要翻译: 用于制备具有广谱活性谱的酸稳定蛋白酶的方法,其以高产率从真菌曲霉的突变体及其中使用的突变体制备。
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公开(公告)号:US4473644A
公开(公告)日:1984-09-25
申请号:US409122
申请日:1982-08-18
IPC分类号: A23K20/189 , C12N1/14 , C12N9/58 , C12R1/845 , C12N15/00
CPC分类号: C12R1/845 , A23K20/189 , C12N9/58 , Y10S435/939
摘要: Process for the preparation of acid stable protease having a broad activity spectrum by culturing selected mutants of Rhizopus rhizopodiformis CBS 227.75 under aerobic conditions in a nutrient medium containing assimilable carbon and nitrogen sources, at a pH between 3 and 7 and at a temperature between 25.degree. C. and 50.degree. C., and separating the resulting protease enzyme; the mutants used in the process together with a process for preparation of the mutants.
摘要翻译: 通过在有氧条件下培养选定的根霉(Rhizopus rhizopodiformis)CBS 227.75的突变体,在含有可吸收的碳源和氮源的营养培养基中,pH为3〜7,温度为25℃,制备具有广谱活性谱的酸稳定蛋白酶的方法 并分离得到的蛋白酶; 在该过程中使用的突变体连同突变体的制备方法。
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公开(公告)号:US4784953A
公开(公告)日:1988-11-15
申请号:US620537
申请日:1984-06-14
申请人: Joachim Schindler , Rolf Schmid
发明人: Joachim Schindler , Rolf Schmid
IPC分类号: C07C59/11 , C07D311/94 , C12P7/40 , C12P7/42 , C12P17/06 , C12R1/01 , C12R1/365 , C12N1/20 , C12N15/00
CPC分类号: C12P17/06 , C12P7/40 , Y10S435/822
摘要: A novel microorganism, mutant chol 149-K14 (DSM 2065) and process for the preparation of 3a.alpha.-H-4.alpha.-(3'-propionic acid)-5.alpha.-hydroxy-7a.beta.-methyl-hexahydro-1-indanone and the .delta.-lactone thereof comprising culturing a steroid compound of animal or plant origin with the above microorganism mutant in an aqueous nutrient medium under aerobic conditions.
摘要翻译: 一种新型微生物突变型胆碱149-K14(DSM 2065)及其制备3aα-H-4α-(3'-丙酸)-5α-羟基-7β-甲基 - 六氢-1-茚酮的方法 和其β-内酯,其包括在有氧条件下在水性营养培养基中培养动物或植物来源的类固醇化合物与上述微生物突变体。
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公开(公告)号:US5352604A
公开(公告)日:1994-10-04
申请号:US33080
申请日:1993-03-10
申请人: Charles R. Wilson , Beth F. Ladin , Jonathan R. Mielenz , Sherman S. M. Hom , Dieter Hansen , Robert B. Reynolds , Nicholas C. T. Kennedy , Joachim Schindler , Michael Bahn , Rolf Schmid , Martina Markgraf , Christian Paech , Karlheinz Maurer
发明人: Charles R. Wilson , Beth F. Ladin , Jonathan R. Mielenz , Sherman S. M. Hom , Dieter Hansen , Robert B. Reynolds , Nicholas C. T. Kennedy , Joachim Schindler , Michael Bahn , Rolf Schmid , Martina Markgraf , Christian Paech , Karlheinz Maurer
IPC分类号: C07K14/195 , C07K14/00 , C07K14/41 , C07K19/00 , C11D3/386 , C12N1/21 , C12N9/54 , C12N9/56 , C12N15/09 , C12N15/57 , C12N15/75 , C12R1/07 , C12R1/10 , C12N15/00 , C12N15/31 , C12N15/74
摘要: A proteolytic enzyme for use in detergent formulations having increased pH and oxidative stability under typical laundering conditions in aqueous solutions is produced by fermenting Bacillus licheniformis host strain transformed by a multicopy plasmid comprised of DNA sequences which code for the desired proteolytic enzyme.
摘要翻译: 通过发酵通过由编码所需蛋白水解酶的DNA序列组成的多拷贝质粒转化的地衣芽孢杆菌宿主菌株产生用于在水溶液中的典型洗涤条件下具有增加的pH和氧化稳定性的洗涤剂制剂中的蛋白水解酶。
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公开(公告)号:US4618582A
公开(公告)日:1986-10-21
申请号:US346591
申请日:1982-02-08
申请人: Konrad Engelskirchen , Werner Stein , Michael Bahn , Ludwig Schieferstein , Joachim Schindler , Rolf Schmid
发明人: Konrad Engelskirchen , Werner Stein , Michael Bahn , Ludwig Schieferstein , Joachim Schindler , Rolf Schmid
CPC分类号: C12P19/06 , Y10S435/91
摘要: In the aerobic culture of microorganisms of the Xanthomonas genus in fermentation media, the use of water-in-oil emulsions in the media minimizes viscosity problems and enhances yields of Xanthomonas biopolymers. Preferably, the emulsion is formed with a surfactant and the microorganism is Xanthomonas campestris.
摘要翻译: 在发酵培养基中黄单胞菌属微生物的需氧培养中,在培养基中使用油包水乳剂使粘度问题最小化并提高黄单胞菌属生物聚合物的产量。 优选地,乳液由表面活性剂形成,微生物是野油菜黄单胞菌(Xanthomonas campestris)。
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公开(公告)号:US09109533B2
公开(公告)日:2015-08-18
申请号:US13636614
申请日:2011-03-29
申请人: Jean-Pierre Budliger , Rolf Schmid
发明人: Jean-Pierre Budliger , Rolf Schmid
CPC分类号: F02G1/0435 , F02G1/043 , F02G1/047 , F02G1/0535 , F02G2243/202 , F02G2244/52 , F02G2253/02 , F02G2253/04 , F02G2270/30 , F02G2270/40 , F02G2270/80 , F02G2280/10 , F02G2280/20
摘要: This Stirling machine comprises a transfer piston (6, 6a) and a moving part (14) of a generator or of an electric motor, the transfer piston (6, 6a) periodically displacing a working gas between an expansion chamber (VE) and a compression chamber (Vc) which chambers are respectively associated with two working faces of the transfer piston (6, 6a) of which the cross-sectional area ratio ac/aE is >0.35 so that its displacement along an axis X oriented towards the expansion volume (VE) generates an in-phase working gas pressure component Px that opposes the displacement of the piston (6, 6a), so that all of the mechanical energy produced is transmitted to the moving part (14). This machine comprises a resonant second piston (10) coupled to the transfer piston (6, 6a) by a quantity of energy that is proportional to the pressure component Px.
摘要翻译: 该斯特林机器包括发电机或电动机的转移活塞(6,6a)和运动部分(14),所述转移活塞(6,6a)周期性地使工作气体在膨胀室(VE)和 压缩室(Vc),其分别与传送活塞(6,6a)的两个工作面相关联,其横截面积比ac / aE大于0.35,使得其沿着朝向膨胀体积的轴线X的位移 (VE)产生与活塞(6,6a)的位移相对的同相工作气体压力分量Px,使得产生的所有机械能传递到运动部件(14)。 该机器包括通过与压力分量Px成比例的一定量的能量耦合到传送活塞(6,6a)的共振第二活塞(10)。
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