公开(公告)号：US07108788B2

公开(公告)日：2006-09-19

申请号：US10487387

申请日：2002-08-29

申请人： Ho Nam Chang ,  Do-Yun Kim ,  Moon-II Kim ,  Chul-Hee Ryu ,  Woo-Gi Lee

发明人： Ho Nam Chang ,  Do-Yun Kim ,  Moon-II Kim ,  Chul-Hee Ryu ,  Woo-Gi Lee

IPC分类号： C02F3/28 ,  B09B3/00

CPC分类号： B09B3/00 ,  C05F9/00 ,  Y02A40/214 ,  Y02W30/47 ,  Y10S210/92 ,  C05F9/02 ,  C05F9/04

摘要： Disclosed is an on-site treatment method of food waste generated from multi-unit dwellings and institutional food service facilities, by transporting wastewater-containing food waste to a separation chamber by use of domestic wastewater or an additional transporter; separating the transported food waste to wastewater and food waste; and treating the separated wastewater in a sewage disposal plant, and the separated food waste using disposal equipment. Such food waste can be treated even using disposal equipment of small capacities, due to separation of wastewater and food waste, compared to disposal equipment of large capacities required for treating wastewater and food waste together. Also, the quality of the separated wastewater is better than the conventional quality of wastewater, and thus loads of the sewage disposal plant can be efficiently decreased.

摘要翻译： 公开了通过使用生活污水或附加运输工具将含废水的食物废物运送到分离室的多单位住房和机构性食品服务设施产生的食品废物的现场处理方法; 将运送的食物废物分解成废水和食物废物; 并处理污水处理厂中分离的废水，以及使用处置设备分离的食物废物。 与处理废水和食物废物所需的大容量的处理设备相比，这种食物废物可以处理甚至使用小容量的处理设备，因为废水和食物废物的分离。 此外，分离废水的质量优于常规废水质量，因此可以有效降低污水处理厂的负荷。

公开(公告)号：US07063968B2

公开(公告)日：2006-06-20

申请号：US10312932

申请日：2001-06-29

申请人： Sang Yup Lee ,  Ho Nam Chang ,  Pyung Cheon Lee ,  Woo-gi Lee

发明人： Sang Yup Lee ,  Ho Nam Chang ,  Pyung Cheon Lee ,  Woo-gi Lee

IPC分类号： C12P7/40 ,  C12P7/54 ,  C12P7/56 ,  C12N1/20

CPC分类号： C12P7/56 ,  C12P7/46 ,  C12P7/54

摘要： A novel microorganism capable of producing organic acids, Mannheimia sp. 55E, and a process for producing organic acid through anaerobic and aerobic incubation using the novel microorganism are provided. The method of producing an organic acid using the microorganism involves incubating Mannheimia sp. 55E with Accession Number KCTC 0769BP in a medium under anaerobic or aerobic conditions and obtaining an organic acid from the medium. Mannheimia sp. 55E produces succinic acid, lactic acid, and formic acid under anaerobic conditions saturated with CO2, and succinic acid, lactic acid, acetic acid, and formic acid under aerobic or N2 anaerobic conditions. Mannheimia sp. 55E is a facultative anaerobe tolerant of oxygen. Thus, the use of Mannheimia sp. 55E in producing organic acids can eliminate a problem of process instability, which would occur by the presence of oxygen in a fermentation process of producing organic acids using an obligate anaerobic microorganism.

摘要翻译： 一种能够生产有机酸的新型微生物，Mannheimia sp。 55E，并且提供了使用新型微生物通过厌氧和需氧培养来生产有机酸的方法。 使用该微生物生产有机酸的方法涉及培养Mannheimia sp。 55E，保藏号为KCTC 0769BP，在厌氧或需氧条件下培养，并从培养基中获得有机酸。 曼海星属 55E在CO 2饱和的厌氧条件下生成琥珀酸，乳酸和甲酸，在需氧或N 2 N下，产生琥珀酸，乳酸，乙酸和甲酸 >厌氧条件。 曼海星属 55E是耐受氧的兼性厌氧菌。 因此，使用曼海星属 55E在生产有机酸中可以消除过程不稳定的问题，这可能是由于在使用专用厌氧微生物生产有机酸的发酵过程中存在氧而发生的。

公开(公告)号：US06406628B1

公开(公告)日：2002-06-18

申请号：US09398390

申请日：1999-09-17

申请人： Ho Nam Chang ,  Seong Jin Lim ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Dong Won Choi

发明人： Ho Nam Chang ,  Seong Jin Lim ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Dong Won Choi

IPC分类号： C02F300

CPC分类号： C02F3/1263 ,  C02F3/308 ,  Y02W10/15

摘要： The present invention provides to a method for removing nitrogen and phosphorous in wastewater simultaneously from wastewater by employing fermentation broth obtained by anaerobic fermentation of organic solid wastes. The method for removing nitrogen and phosphorous in wastewater of the invention comprises the steps of subjecting organic waste material to anaerobic fermentation at 30 to 40° C. for 2 to 4 days by using anaerobic sludge to obtain fermentation broth; and, adding the fermentation broth to a sequencing batch reactor with alternating anaerobic-aerobic-anoxic cycles under anaerobic and anoxic conditions in a separate manner. In accordance with the present invention, nitrogen in the influent wastewater can be removed more than 95% in total and phosphorous concentration can be kept below 1 ppm regardless of the concentration of organic materials in the influent wastewater. Further, the present method requires no extra pretreatment steps or chemicals which are essential to remove residual ammonia nitrogen in the prior art systems, and lowers the overall cost for wastewater treatment due to the usage of organic waste materials.

摘要翻译： 本发明提供一种通过使用通过有机固体废物的厌氧发酵获得的发酵液，从废水中同时除去废水中的氮和磷的方法。 本发明废水中除氮和磷的方法包括以下步骤：通过使用厌氧污泥将有机废物在30〜40℃进行厌氧发酵2〜4天，得到发酵液; 并将发酵液添加到在厌氧和缺氧条件下以分开的方式交替的厌氧 - 需氧 - 缺氧循环的测序间歇反应器。 根据本发明，流入废水中的氮总量可以去除95％以上，磷浓度可以保持在1ppm以下，而与进水废水中有机物质的浓度无关。 此外，本方法不需要额外的预处理步骤或在现有技术系统中除去残余氨氮所必需的化学品，并且由于使用有机废物材料而降低了废水处理的总体成本。

公开(公告)号：US06596521B1

公开(公告)日：2003-07-22

申请号：US09529570

申请日：2000-04-13

申请人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

发明人： Ho Nam Chang ,  Yong Keun Chang ,  Sun Hoon Kwon ,  Woo Gi Lee ,  Pyung Cheon Lee ,  Ik Keun Yoo ,  Seong Jin Lim

IPC分类号： C12P740

CPC分类号： C12P7/46 ,  C12M23/58 ,  C12M29/18 ,  C12M41/26 ,  C12P7/40 ,  C12P7/42 ,  C12P7/48

摘要： The present invention provides a method for manufacturing organic acid by high-efficiency fermentation, which comprises the steps of continuous culture of organic acid bacteria and collection of organic acid produced from the culture employing a cell-recycle multiple-stage continuous fermentor with serially connected fermentors, each of which comprises a fermentor containing a ferment container, temperature controller, stirrer, and pH controller; pumps for efflux-circulation of media from the fermentor; and, cell separator for separation and circulation of media from the pumps. According to the present method, the high-concentration lactic acid of 90 g/L can be produced with the high productivity of 50 g/L/h, which can reduce the facility cost and production cost in the bulk manufacturing process. In addition, the present invention can be effectively applied to the production of other organic acids such as acetic acid, formic acid, citric acid, malic acid, maleic acid, fumaric acid, and succinic acid, which show the end-product inhibition.

摘要翻译： 本发明提供了一种通过高效发酵制造有机酸的方法，其包括以下步骤：连续培养有机酸细菌和收集由使用连续发酵罐的细胞循环多级连续发酵罐的培养物产生的有机酸 ，其中每个包含含有发酵容器，温度控制器，搅拌器和pH控制器的发酵罐; 用于来自发酵罐的介质的外排循环的泵; 以及用于从泵分离和循环介质的细胞分离器。 根据本方法，可以以50g / L / h的高生产率生产90g / L的高浓度乳酸，这可以降低批量制造过程中的设备成本和生产成本。 此外，本发明可以有效地应用于生产其它有机酸如乙酸，甲酸，柠檬酸，苹果酸，马来酸，富马酸和琥珀酸，这些有机酸显示出最终产物的抑制作用。

公开(公告)号：US06204046B1

公开(公告)日：2001-03-20

申请号：US09261838

申请日：1999-03-03

申请人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

发明人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

IPC分类号： C12N120

CPC分类号： C12R1/01 ,  C10G32/00

摘要： The present invention relates to Gordona sp. CYKS1 (KCTC 0431BP) capable of selective removal of organically bound sulfurs from carbonaceous fossil fuel such as petroleum and coal by cleaving bonds between carbon and sulfur atoms in the said sulfur-containing organic compounds, and a method for biological desulfurization using this strain at the room temperature and atmospheric pressure. Since Gordona sp. CYKS1 (KCTC 0431BP) utilizes various organic sulfur compounds in fossil fuel besides dibenzothiophene as a sole sulfur source, the method for biological desulfurization employing the Gordona strain has advantages over the conventional chemical methods as followings: The desulfurization can be carried out at a mild condition; the cost for installation and operation of equipments can be reduced; and, the desulfurization of highly complex organic sulfur compounds can be realized.

摘要翻译： 本发明涉及Gordona sp。 CYKS1（KCTC 0431BP）能够通过裂解所述含硫有机化合物中的碳原子和硫原子之间的键，从含碳化石燃料如石油和煤中有机结合的硫选择性地除去，以及使用该菌株在生物脱硫中的方法 室温和大气压。 由于戈多纳 CYKS1（KCTC 0431BP）除二苯并噻吩以外的化石燃料中使用各种有机硫化合物作为唯一的硫源，使用戈多纳菌的生物脱硫方法比以往的化学方法具有以下优点：脱硫可在温和条件下进行 ; 降低设备安装运行成本; 并且可以实现高度复杂的有机硫化合物的脱硫。

公开(公告)号：US07803587B2

公开(公告)日：2010-09-28

申请号：US11743668

申请日：2007-05-02

申请人： Sang Yup Lee ,  Ho Nam Chang ,  Hyohak Song ,  Tae Yong Kim ,  Bo-Kyung Choi

发明人： Sang Yup Lee ,  Ho Nam Chang ,  Hyohak Song ,  Tae Yong Kim ,  Bo-Kyung Choi

IPC分类号： C12P7/44 ,  C12N1/20 ,  C12Q1/00 ,  C12Q1/68 ,  G06G7/48

CPC分类号： C12N1/20 ,  C12N5/0018 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/40 ,  C12P7/46 ,  G06F19/12

摘要： A method for developing a culture medium using genome information and in silico analysis. In one implementation, the method involves developing a minimal synthetic medium, including the steps of constructing a metabolic network using genome information of prokaryotic cell or eukaryotic cell, selecting components of the minimal synthetic medium removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation, and determining a final culture medium by actual culture.

摘要翻译： 使用基因组信息和计算机分析开发培养基的方法。 在一个实施方案中，该方法包括开发最小的合成培养基，包括使用原核细胞或真核细胞的基因组信息构建代谢网络的步骤，从构建的代谢网络中选择去除外部代谢物中的任何一种的最小合成培养基的组分，以及 使用计算机模拟进行代谢通量分析，并通过实际培养确定最终培养基。

公开(公告)号：US20100041124A1

公开(公告)日：2010-02-18

申请号：US12523279

申请日：2008-01-24

申请人： Ho Nam Chang ,  Byoungjin Kim ,  Changmoon Jeong ,  Jong Won Kang ,  Joongkon Park

发明人： Ho Nam Chang ,  Byoungjin Kim ,  Changmoon Jeong ,  Jong Won Kang ,  Joongkon Park

IPC分类号： C12N1/00 ,  C12M1/14

CPC分类号： C12M27/02 ,  C12M23/58 ,  C12M25/18

摘要： The present invention relates to a single CSTR bioreactor equipped with a cell recycle unit, and a multi-stage CSTR bioreactor system in which 2-7 such single CSTR bioreactors are connected in series with each other. According to the present invention, multi-stage fermentation is carried out in the bioreactor equipped with the cell recycle unit, and thus the production of products (alcohols, organic acids, antibiotics, recombinant proteins, etc.) by anaerobic or aerobic microbial fermentation can be increased, and in addition, a final product can be obtained at high concentration, thus improving the productivity and economic efficiency of a microbial process.

摘要翻译： 本发明涉及装备有细胞再循环单元的单个CSTR生物反应器和多级CSTR生物反应器系统，其中2-7个这样的单个CSTR生物反应器彼此串联连接。 根据本发明，在配备有细胞循环装置的生物反应器中进行多级发酵，因此通过厌氧或需氧微生物发酵生产产物（醇，有机酸，抗生素，重组蛋白等）可以 并且另外可以以高浓度获得最终产品，从而提高微生物过程的生产率和经济效率。

公开(公告)号：US06197570B1

公开(公告)日：2001-03-06

申请号：US09260979

申请日：1999-03-02

申请人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

发明人： Yong Keun Chang ,  Ho Nam Chang ,  Sung-Keun Rhee ,  Je Hwan Chang ,  Jung Hyun Sung

IPC分类号： C12N120

CPC分类号： C12R1/365 ,  C10G32/00

摘要： The present invention relates to Nocardia sp. CYKS2 (KCTC 0432 Br) capable of selective removal of organically bound sulfurs from carbonaceous fossil fuel such as petroleum and coal by cleaving bonds between carbon and sulfur atoms in the said sulfur-containing organic compounds, and a method for biological desulfurization using this strain at the room temperature and atmospheric pressure. Since Nocardia sp. CYKS2 (KCTC 0432 Bp) utilizes various organic sulfur compounds in fossil fuel besides dibenzothiophene as a sole sulfur source, the method for biological desulfurization employing the Nocardia strain has advantages over the conventional chemical methods as followings: The desulfurization can be carried out at a mild condition; the cost for installation and operation of equipments can be reduced; and, the desulfurization of highly complex organic sulfur compounds can be realized.

摘要翻译： 本发明涉及诺卡氏菌属 CYKS2（KCTC 0432 Br）能够通过在所述含硫有机化合物中裂解碳和硫原子之间的键，从含碳化石燃料如石油和煤中有机结合的硫选择性除去，以及使用该菌株的生物脱硫方法 室温和大气压力。 自诺卡氏菌属 CYKS2（KCTC 0432 Bp）除了二苯并噻吩以外，在化石燃料中使用各种有机硫化合物作为唯一的硫源，采用诺卡氏菌菌株的生物脱硫方法比以往的化学方法具有以下优点：脱硫可以在温和 条件; 降低设备安装运行成本; 并且可以实现高度复杂的有机硫化合物的脱硫。

公开(公告)号：US4910139A

公开(公告)日：1990-03-20

申请号：US226718

申请日：1988-08-01

申请人： Ho Nam Chang ,  Bong Hyun Chung

发明人： Ho Nam Chang ,  Bong Hyun Chung

IPC分类号： C12M1/00 ,  C12M1/12 ,  C12P7/48 ,  C12R1/645 ,  C12R1/685

CPC分类号： C12R1/685 ,  C12M25/10 ,  C12M29/16 ,  C12P7/48 ,  Y10S435/917

摘要： Aspergillus niger B60 was immobilized in a dual hollow fiber bioreactor to produce citric acid continuously. The fungi proliferated well in the interstitial region formed by a parallel arrangement of three microporous polypropylene hollow fibers contained within a silicone tube. Excessive fungal cell growth could be controlled by supplying a nitrogen-deficient medium at the production stage, and also uniform cell distribution in the reactor could be obtained by changing the direction of medium flow. With pure oxygen aeration and nitrogen-deficient medium, volumetric productivity reached 1.62 g/l.h at a residence time of 4.02 h, which corresponded to a 27-fold increase over that of shake-flask fermentation. When the residence time was increased to 20.1 h, citric acid at a concentration of 26 g/l was continuously produced, with a yield of 80-90% and a volumetric productivity of 1.3 g/l.h. This represents a significant improvement in final concentration, yield and the volumetric productivity over the equivalent values of the corresponding batch formentation, which were 18 g/l, 40% and 0.06 g/l.h, respectively.

摘要翻译： 将黑曲霉B60固定在双中空纤维生物反应器中以连续生产柠檬酸。 真菌在由硅胶管中包含的三个微孔聚丙烯中空纤维的平行布置形成的间隙区域中增殖良好。 过量的真菌细胞生长可以通过在生产阶段供应缺氮培养基来控制，并且可以通过改变培养基流动的方向来获得反应器中均匀的细胞分布。 在纯氧曝气和氮缺乏培养基中，停留时间为4.02 h，体积生产率达到1.62 g / l.h，与摇瓶发酵相比，增加了27倍。 当停留时间增加到20.1小时时，连续生产浓度为26g / l的柠檬酸，产率为80-90％，体积生产率为1.3g / l.h。 这代表了相对于相应的批次补偿的最终浓度，产量和体积生产率的显着改善，分别为18g / l，40％和0.06g / l.h。