公开(公告)号：US06916666B1

公开(公告)日：2005-07-12

申请号：US09582741

申请日：1998-12-30

申请人： Ib Mendel-Hartvig ,  Jörgen Gustafsson

发明人： Ib Mendel-Hartvig ,  Jörgen Gustafsson

IPC分类号： G01N33/543 ,  G01N33/53 ,  G01N33/558 ,  G01N33/566

CPC分类号： G01N33/558

摘要： A lateral flow method for the determination of an analyte in a sample utilizes biospecific affinity reactions. The method comprises forming a complex comprising Reactant I - - - Analyte′ - - - Reactant* wherein Reactant* and Reactant I exhibit biospecific affinity to the analyte, Reactant* is analytically detectable, and Analyte′ is the analyte or an analyte-related reactant, determining a detectable signal from Reactant* in the complex (sample value), and obtaining the amount of analyte in a sample by comparing the sample value with one or more calibrator values, each of which corresponds to a standard amount of analyte. Before determination of the calibrator value, either calibrator or a binder for the calibrator has been bound to a matrix and is released at the determination of calibrator value. The calibrator and the analyte have the ability to biospecifically bind to Reactant* via equivalent binding sites, and one or more calibrator zones comprising calibrator or binder for the calibrator are located in the same process flow as Reactant I in a detection zone. A device for transforming measured signal values for a completed, analytically detectable reactant (Reactant*) to real amounts of analyte in a sample comprises a flow matrix in which there is an area of process flow for the transport of Reactant*, having one or more calibrator zones, an application zone for Reactant* upstream of the one or more calibrator zones, and one or more detection zones downstream of the one or more calibrator zones.

摘要翻译： 用于测定样品中的分析物的侧流方法利用生物特异性亲和反应。 该方法包括形成包含反应物I - - - 分析物' - - - 反应物*的复合物，其中反应物*和反应物I对分析物显示生物特异性亲和力，反应物*是可分析检测的，分析物是分析物或分析物相关反应物 ，在复合物（样本值）中确定来自反应物*的可检测信号，并通过将样品值与一个或多个校准值进行比较来获得样品中的分析物的量，每个校正值各自对应于标准量的分析物。 在确定校准器值之前，校准器或校准器的粘合剂已经绑定到矩阵，并在确定校准器值时释放。 校准器和分析物具有通过等效结合位点生物特异性结合反应物*的能力，并且包含用于校准器的校准器或粘合剂的一个或多个校准区位于与检测区中的反应物I相同的工艺流程中。 用于将完整的可分析检测的反应物（反应物*）的测量信号值转换成样品中实际分析物的装置包括流动基质，其中存在用于运输反应物*的工艺流程的区域，具有一个或多个 校准器区域，反应物的应用区域*一个或多个校准器区域的上游，以及一个或多个校准器区域下游的一个或多个检测区域。

公开(公告)号：US07405084B1

公开(公告)日：2008-07-29

申请号：US09582808

申请日：1998-12-30

申请人： Ib Mendel-Hartvig ,  Lena Vinterbäck ,  Ann Jonsson ,  Jörgen Gustafsson

发明人： Ib Mendel-Hartvig ,  Lena Vinterbäck ,  Ann Jonsson ,  Jörgen Gustafsson

IPC分类号： G01N33/543

CPC分类号： G01N33/558 ,  Y10S435/97

摘要： A method for use in a flow matrix, which utilizes biospecific affinity reactions to detect an analyte in the sample, and which comprises allowing the sample comprising the analyte and an analytically detectable reactant (Reactant*) to migrate through flow channels in a flow matrix to a detection zone located in the matrix, in which there is a firmly anchored biospecific affinity reactant (Capturer), and capturing the Reactant* in the detection zone in an amount related to the amount of analyte in the sample. The Reactant* has labeled particles of an analytically detectable group, and the Capturer is anchored to the matrix by immobilized particles which exhibit hydrophilic groups on their surface. A test kit comprises a flow matrix having a detection zone in which there is a firmly anchored biospecific affinity reactant (Capturer), and an analytically detectable reactant (Reactant*). The Reactant* has labeled particles of an analytically detectable group, and the Capturer is anchored to the matrix by immobilized particles which exhibit hydrophilic groups on their surface.

摘要翻译： 一种在流动基质中使用的方法，其利用生物特异性亲和反应来检测样品中的分析物，并且包括使包含分析物的样品和可分析检测的反应物（反应物*）通过流动基质中的流动通道迁移至 位于基质中的检测区，其中存在牢固锚定的生物特异性亲和反应物（捕获器），并且以与样品中分析物的量相关的量在检测区捕获反应物*。 反应物*已经标记了可分析可检测的基团的颗粒，并且捕获器通过在其表面上显示亲水基团的固定化颗粒锚定到基质上。 测试试剂盒包括具有检测区域的流动基质，其中存在牢固锚定的生物特异性亲和反应物（捕获器）和可分析检测的反应物（反应物*）。 反应物*已经标记了可分析可检测的基团的颗粒，并且捕获器通过在其表面上显示亲水基团的固定化颗粒锚定到基质上。

公开(公告)号：US09347931B2

公开(公告)日：2016-05-24

申请号：US13265813

申请日：2010-04-23

申请人： Anthony Joseph Killard ,  Magdalena Maria Dudek ,  Brian MacCraith ,  Ib Mendel-Hartvig ,  Ove Öhman

发明人： Anthony Joseph Killard ,  Magdalena Maria Dudek ,  Brian MacCraith ,  Ib Mendel-Hartvig ,  Ove Öhman

IPC分类号： G01N33/00 ,  G01N33/49 ,  G01N33/543 ,  G01N33/545 ,  G01N33/558 ,  G01N33/86 ,  B01L3/00

CPC分类号： G01N33/4905 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/086 ,  G01N33/54353 ,  G01N33/545 ,  G01N33/558 ,  G01N33/86

摘要： The present invention is directed to a lateral flow assay device for the monitoring and measuring of coagulation and method thereof. Ideally, the invention is directed to a lateral capillary flow device for the monitoring and/or measurement of coagulation in a liquid sample wherein the device comprises a non-porous substrate with a zone for receiving a sample and a defined flow path zone wherein a clotting agent is deposited on at least part of the defined flow path zone to accelerate the coagulation of the liquid sample, enable the formation of an evenly distributed clot along the defined flow path zone and to result in the change in flow rate or cessation of flow of the liquid sample along the defined flow path zone.

摘要翻译： 本发明涉及一种用于监测和测量凝血的侧流测定装置及其方法。 理想地，本发明涉及用于监测和/或测量液体样品中的凝结的侧向毛细管流动装置，其中该装置包括具有用于接收样品的区域和限定的流动路径区域的无孔基材，其中凝固 试剂沉积在限定的流动路径区的至少部分上以加速液体样品的凝结，使得能够沿着限定的流动路径区形成均匀分布的凝块，并导致流速的变化或流动停止 液体样品沿着限定的流路区域。

公开(公告)号：US20120107851A1

公开(公告)日：2012-05-03

申请号：US13265813

申请日：2010-04-23

申请人： Anthony Joseph Killard ,  Magdalena Maria Dudek ,  Brian MacCraith ,  Ib Mendel-Hartvig ,  Ove Öhman

发明人： Anthony Joseph Killard ,  Magdalena Maria Dudek ,  Brian MacCraith ,  Ib Mendel-Hartvig ,  Ove Öhman

IPC分类号： C12Q1/56 ,  B05D3/04 ,  G01N33/86 ,  G01N33/68 ,  C12M1/40

CPC分类号： G01N33/4905 ,  B01L3/502707 ,  B01L3/502753 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/086 ,  G01N33/54353 ,  G01N33/545 ,  G01N33/558 ,  G01N33/86

摘要： The present invention is directed to a lateral flow assay device for the monitoring and measuring of coagulation and method thereof. Ideally, the invention is directed to a lateral capillary flow device for the monitoring and/or measurement of coagulation in a liquid sample wherein the device comprises a non-porous substrate with a zone for receiving a sample and a defined flow path zone wherein a clotting agent is deposited on at least part of the defined flow path zone to accelerate the coagulation of the liquid sample, enable the formation of an evenly distributed clot along the defined flow path zone and to result in the change in flow rate or cessation of flow of the liquid sample along the defined flow path zone.

摘要翻译： 本发明涉及一种用于监测和测量凝血的侧流测定装置及其方法。 理想地，本发明涉及用于监测和/或测量液体样品中的凝结的侧向毛细管流动装置，其中该装置包括具有用于接收样品的区域和限定的流动路径区域的无孔基材，其中凝固 试剂沉积在限定的流动路径区的至少部分上以加速液体样品的凝结，使得能够沿着限定的流动路径区形成均匀分布的凝块，并导致流速的变化或流动停止 液体样品沿着限定的流路区域。

公开(公告)号：US20100248394A1

公开(公告)日：2010-09-30

申请号：US12796351

申请日：2010-06-08

申请人： Per Ove Ohman ,  Ib Mendel-Hartvig

发明人： Per Ove Ohman ,  Ib Mendel-Hartvig

IPC分类号： G01N33/543

CPC分类号： B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0442 ,  B01L2400/086 ,  Y10T436/2575

摘要： A device for handling liquid samples, comprising a flow path with at least one zone for receiving the sample, and a transport or incubation zone, said zones connected by or comprising an area having projections substantially vertical to its surface, said device provided with a sink with a capacity of receiving said liquid sample, said sink comprising an area having projections substantially vertical to its surface, and said sink being adapted to respond to an external influence regulating its capacity to receive said liquid sample.

摘要翻译： 一种用于处理液体样品的装置，包括具有用于接收样品的至少一个区域的流动路径，以及运输或孵化区域，所述区域由具有基本上垂直于其表面的突起的区域连接或包括具有突出部的区域，所述装置设置有水槽 具有接收所述液体样品的能力，所述水槽包括具有基本上垂直于其表面的突起的区域，并且所述水槽适于响应于调节其接收所述液体样品的能力的外部影响。

公开(公告)号：US20060285996A1

公开(公告)日：2006-12-21

申请号：US11471061

申请日：2006-06-19

申请人： Per Ohman ,  Ib Mendel-Hartvig

发明人： Per Ohman ,  Ib Mendel-Hartvig

IPC分类号： G01N31/22

CPC分类号： B01L3/5025 ,  B01L3/5023 ,  B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/086 ,  Y10T436/2575

摘要： An absorbing zone for establishing and/or maintaining fluid transport through or along said at least one fluid passage is manufactured on the basis of a non-porous substrate, having projections substantially perpendicular to said surface, and said projections having a height, diameter and a distance or distances between the projections such, that lateral capillary flow of said fluid in said zone is achieved.

摘要翻译： 用于建立和/或维持沿着所述至少一个流体通道的流体输送的吸收区域是基于具有基本上垂直于所述表面的突出物的无孔基材制造的，并且所述突起具有高度，直径和 突起之间的距离或距离，使得实现所述区域中的所述流体的侧向毛细管流动。

公开(公告)号：US20060239859A1

公开(公告)日：2006-10-26

申请号：US10560214

申请日：2005-05-26

申请人： Per Ohman ,  Ib Mendel-Hartvig

发明人： Per Ohman ,  Ib Mendel-Hartvig

IPC分类号： B01L3/00

CPC分类号： B01L3/502746 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0433 ,  B01L2400/0442 ,  B01L2400/086 ,  Y10T436/2575

摘要： A device for handling liquid samples, comprising a flow path with at least one zone for receiving the sample, and a transport or incubation zone, said zones connected by or comprising an area having projections substantially vertical to its surface, said device provided with a sink with a capacity of receiving said liquid sample, said sink comprising an area having projections substantially vertical to its surface, and said sink being adapted to respond to an external influence regulating its capacity to receive said liquid sample.

摘要翻译： 一种用于处理液体样品的装置，包括具有用于接收样品的至少一个区域的流动路径，以及运输或孵化区域，所述区域由具有基本上垂直于其表面的突起的区域连接或包括具有突出部的区域，所述装置设置有水槽 具有接收所述液体样品的能力，所述水槽包括具有基本上垂直于其表面的突起的区域，并且所述水槽适于响应于调节其接收所述液体样品的能力的外部影响。

公开(公告)号：US08877142B2

公开(公告)日：2014-11-04

申请号：US12305630

申请日：2007-06-20

申请人： Per Ove Öhman ,  Ib Mendel-Hartvig

发明人： Per Ove Öhman ,  Ib Mendel-Hartvig

IPC分类号： B01L3/00 ,  G01N33/52 ,  G01N33/558 ,  G01N35/00

CPC分类号： G01N33/5304 ,  B01L3/5023 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502746 ,  B01L2300/0681 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0825 ,  B01L2300/0896 ,  B01L2400/0406 ,  B01L2400/086 ,  B01L2400/088 ,  G01N33/525 ,  G01N33/558 ,  G01N2035/00237 ,  Y10T436/25

摘要： There is provided an assay device comprising a lid and a base, said base comprising, a sample addition zone, a reaction zone and an absorbing zone, said components being in fluid connection and being part of a fluid passage leading from the sample addition zone to the absorbing zone, wherein: (a) a sample addition well is integrated in the lid, (b) the absorbing zone consists of an area on an non-porous substrate, having substantially perpendicular projections, said projections defining a volume, which together with the volume of the fluid passage defines the sample volume subjected to the assay, and (c) at least one filter is between the sample addition well and the sample addition zone. There is further provided methods for handling samples.

摘要翻译： 提供了一种包括盖和基底的测定装置，所述基底包括样品加入区，反应区和吸收区，所述组分处于流体连接状态，并且是从样品添加区引导至流体通道的一部分 所述吸收区，其中：（a）样品添加井被整合在所述盖中，（b）所述吸收区由无孔基底上的区域组成，具有基本上垂直的突起，所述突起限定体积， 流体通道的体积限定经过测定的样品体积，和（c）至少一个过滤器位于样品加入孔和样品添加区之间。 还提供了处理样品的方法。

公开(公告)号：US08025854B2

公开(公告)日：2011-09-27

申请号：US10492453

申请日：2003-06-04

申请人： Ove Ohman ,  Ib Mendel-Hartvig

发明人： Ove Ohman ,  Ib Mendel-Hartvig

IPC分类号： B01L3/00

CPC分类号： B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/086 ,  G01N30/6065 ,  G01N30/6095 ,  G01N2030/525 ,  Y10T436/25

摘要： The invention relates to a micro fluidic system comprising a substrate, and, provided on said substrate, at least one flow path interconnecting with functional means in which liquid samples can be treated by desired procedures. The flow paths are laid out to form a pattern for the transport of liquid samples to and from said functional means. These flow paths comprise a plurality of micro posts protruding upwards from said substrate, the spacing between the micro posts being small enough to induce a capillary action in a liquid sample applied anywhere within any of said flow paths, so as to force said liquid to move from where said liquid sample was applied.

摘要翻译： 本发明涉及一种微流体系统，其包括衬底，并且在所述衬底上提供与功能装置相互连接的至少一个流路，其中可以通过期望的程序处理液体样品。 布置流动路径以形成用于将液体样品运送到所述功能装置和从所述功能装置输送液体样品的图案。 这些流动路径包括从所述基底向上突出的多个微柱，微柱之间的间隔足够小，以在任何一个所述流动路径内的任何地方施加液体样品中的毛细管作用，以迫使所述液体移动 从其中应用所述液体样品。

公开(公告)号：US20100041154A1

公开(公告)日：2010-02-18

申请号：US12305641

申请日：2007-06-20

申请人： Per Ove Öhman ,  Ib Mendel-Hartvig ,  Tomas Lindström

发明人： Per Ove Öhman ,  Ib Mendel-Hartvig ,  Tomas Lindström

IPC分类号： G01N33/558 ,  G01N33/00

CPC分类号： G01N33/543 ,  B01L3/502746 ,  B01L2200/025 ,  B01L2300/025 ,  B01L2300/0825 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/086 ,  G01N33/558 ,  Y10S436/807 ,  Y10T436/10 ,  Y10T436/13

摘要： The present invention provides an assay device for performing an assay on a liquid sample using a detection conjugate capable of binding to an antigen and containing a label, said device comprising a substrate having a substrate surface, wherein the surface comprises projections substantially perpendicular to said surface, said projections having a height, diameter and distance capable of generating lateral capillary flow of a fluid in said passage, said surface comprising a sample addition zone, a reaction zone and an absorbing zone, said zones being connected by at least one fluid passage, wherein said device has a first functionality verifying feature located between the sample addition zone and the reaction zone, and a second functionality verifying feature located within the absorbing zone, and both functionality verifying features being features capable of undergoing a detectable change when contacted by the sample, said assay device further comprising at least one alignment verification zone. There is further provided a kit of parts and a method of conducting an assay.

摘要翻译： 本发明提供了一种用于使用能够结合抗原并含有标签的检测结合物对液体样品进行测定的测定装置，所述装置包括具有基底表面的基底，其中所述表面包括基本垂直于所述表面的突起 所述突起具有能够在所述通道中产生流体的侧向毛细管流动的高度，直径和距离，所述表面包括样品添加区，反应区和吸收区，所述区通过至少一个流体通道连接， 其中所述装置具有位于样品添加区域和反应区域之间的第一功能验证特征，以及位于吸收区域内的第二功能验证特征，并且两个功能性验证特征是当被样品接触时能够经受可检测变化的特征 ，所述测定装置还包括至少一个对准验证码 定位区。 还提供了一组试剂盒和进行测定的方法。