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19 条记录 (耗时 0.034 秒)

    METHODS, DEVICES, AND SYSTEMS FOR FLUID MIXING AND CHIP INTERFACE
    1.
    发明申请
    METHODS, DEVICES, AND SYSTEMS FOR FLUID MIXING AND CHIP INTERFACE 有权
    用于流体混合和芯片接口的方法，装置和系统

    公开(公告)号：US20120058571A1

    公开(公告)日：2012-03-08

    申请号：US13222450

    申请日：2011-08-31

    申请人： Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

    发明人： Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

    IPC分类号： G01N1/00 ,  B01L3/02

    CPC分类号： B01L3/022 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2300/14 ,  B01L2400/0442 ,  B01L2400/0487 ,  Y10T436/2575

    摘要： In one aspect, the present invention provides methods, devices, and systems for ensuring that multiple components of a mixture are fully mixed in a continuous flow microfluidic system while ensuring that mixing between segments flowing through the chip is minimized. In some embodiments, the present invention includes mixing fluids in a droplet maintained at the tip of a pipette before the mixture is introduced to the microfluidic device. In another aspect, the present invention provides a pipette tip having a ratio of an outside diameter to an inside diameter that provides sufficient surface area for a droplet comprising up to the entire volume of the liquid to suspend from the pipette tip intact. In yet another aspect, the present invention provides methods, devices, and systems for delivering a reaction mixture to a microfluidic chip comprising a docking receptacle, an access tube and a reservoir.

    摘要翻译： 一方面，本发明提供用于确保混合物的多个组分在连续流微流体系统中完全混合的方法，装置和系统，同时确保流过芯片的区段之间的混合最小化。 在一些实施方案中，本发明包括在将混合物引入微流体装置之前将保持在移液管尖端的液滴混合流体。 在另一方面，本发明提供了一种移液管尖端，其具有外径与内径之比，其为包含液体的整个体积的液滴提供足够的表面面积，从而完全悬浮于移液管尖端。 在另一方面，本发明提供用于将反应混合物输送到微流控芯片的方法，装置和系统，所述微流控芯片包括对接插座，进入管和储存器。

    METHOD, DEVICES, AND SYSTEMS FOR FLUID MIXING AND CHIP INTERFACE
    4.
    发明申请
    METHOD, DEVICES, AND SYSTEMS FOR FLUID MIXING AND CHIP INTERFACE 审中-公开
    用于流体混合和芯片接口的方法，装置和系统

    公开(公告)号：US20120058519A1

    公开(公告)日：2012-03-08

    申请号：US13222474

    申请日：2011-08-31

    申请人： Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

    发明人： Ivor T. Knight ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy

    IPC分类号： C12P19/34 ,  C12M1/00

    CPC分类号： B01L3/022 ,  B01L3/5025 ,  B01L3/502715 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/10 ,  B01L2300/14 ,  B01L2400/0442 ,  B01L2400/0487 ,  Y10T436/2575

    摘要： In one aspect, the present invention provides methods, devices, and systems for ensuring that multiple components of a mixture are fully mixed in a continuous flow microfluidic system while ensuring that mixing between segments flowing through the chip is minimized. In some embodiments, the present invention includes mixing fluids in a droplet maintained at the tip of a pipette before the mixture is introduced to the microfluidic device. In another aspect, the present invention provides methods, devices, and systems for creating segments that move through a microfluidic chip with minimal mixing between segments. The microfluidic chip may have an interface chip and a reaction chip. In some embodiments, the present invention includes creating segments that flow through an interface chip and a reaction chip, wherein the interface chip and a reaction chip have separate flow control mechanisms and produce minimal mixing between segments.

    摘要翻译： 一方面，本发明提供用于确保混合物的多个组分在连续流微流体系统中完全混合的方法，装置和系统，同时确保流过芯片的区段之间的混合最小化。 在一些实施方案中，本发明包括在将混合物引入微流体装置之前将保持在移液管尖端的液滴混合流体。 在另一方面，本发明提供用于创建通过微流体芯片移动的段的方法，装置和系统，其中片段之间的混合最小。 微流控芯片可以具有接口芯片和反应芯片。 在一些实施例中，本发明包括创建流过界面芯片和反应芯片的区段，其中界面芯片和反应芯片具有分离的流量控制机制并且在区段之间产生最小的混合。

    Device and method for digital multiplex PCR assays
    6.
    发明授权
    Device and method for digital multiplex PCR assays 有权
    数字多重PCR测定的装置和方法

    公开(公告)号：US09114398B2

    公开(公告)日：2015-08-25

    申请号：US11947227

    申请日：2007-11-29

    申请人： Ivor T. Knight ,  Hiroshi Inoue

    发明人： Ivor T. Knight ,  Hiroshi Inoue

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  B01L7/00 ,  B01L3/00 ,  G01N21/64 ,  G06F19/22 ,  G01N21/03 ,  G01N21/05

    CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2200/147 ,  B01L2300/0864 ,  C12Q1/686 ,  G01N21/0332 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/0346 ,  G06F19/22 ,  C12Q2565/629 ,  C12Q2561/113

    摘要： A method and device for digital multiplex PCR assays employ a microfluidic chip for performing real-time, continuous flow PCR within microchannels of the chip. A stream of sample material is introduced into each microchannel and alternating boluses of assay-specific reagents and buffer are introduced into the stream to form sequentially configured test boluses. A PCR procedure is performed on the test boluses followed by a thermal melt procedure. During the thermal melt procedure, fluorescent output is detected and fluorescence vs temperature data is collected and compared to expected normal correlations. The results, positive or negative, are converted to digital format, with positive results designated as “1” and negative results designated as “0”, or vice versa.

    摘要翻译： 用于数字多重PCR测定的方法和装置使用微流体芯片来在芯片的微通道内进行实时连续流动PCR。 将样品材料流引入每个微通道中，并将测定特异性试剂和缓冲液的交替喷射引入流中以形成顺序配置的测试推注。 在测试团块上进行PCR程序，然后进行热熔融程序。 在热熔化过程中，检测荧光输出，并收集荧光对温度数据并与预期的正常相关性进行比较。 结果，正或负转换为数字格式，阳性结果指定为“1”，负结果指定为“0”，反之亦然。

    METHOD AND APPARATUS FOR APPLYING CONTINUOUS FLOW AND UNIFORM TEMPERATURE TO GENERATE THERMAL MELTING CURVES IN A MICROFLUIDIC DEVICE
    8.
    发明申请
    METHOD AND APPARATUS FOR APPLYING CONTINUOUS FLOW AND UNIFORM TEMPERATURE TO GENERATE THERMAL MELTING CURVES IN A MICROFLUIDIC DEVICE 有权
    用于连续流动和均匀温度在微流体装置中生成热熔融曲线的方法和装置

    公开(公告)号：US20070231799A1

    公开(公告)日：2007-10-04

    申请号：US11381896

    申请日：2006-05-05

    申请人： Ivor T. Knight ,  Deborah J. Boyles

    发明人： Ivor T. Knight ,  Deborah J. Boyles

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  B01L3/502715 ,  B01L7/525 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2300/1833 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/06

    摘要： The present invention provides a method for performing thermal melt analysis using a microfluidic device, the method comprising providing a microfluidic device having at least one microfluidic channel, introducing fluid comprising into the at least one microfluidic channel, continuously flowing the fluid through the at least one microfluidic channel while varying the temperature of the entire fluid stream as it moves through the at least one microfluidic channel by uniformly heating the entire fluid stream, and measuring, while continuously flowing the fluid through the at least one microfluidic channel, a detectable property emanating from the fluid,

    摘要翻译： 本发明提供一种使用微流体装置进行热熔融分析的方法，所述方法包括提供具有至少一个微流体通道的微流体装置，将包含至少一个微流体通道的流体引入至少一个流体 微流体通道，同时通过均匀加热整个流体流来改变整个流体流的温度，同时在流体连续流过至少一个微流体通道的过程中测量可检测的特性， 流体，

    Chip and cartridge design configuration for performing micro-fluidic assays
    10.
    发明授权
    Chip and cartridge design configuration for performing micro-fluidic assays 有权
    用于进行微流体测定的芯片和墨盒设计配置

    公开(公告)号：US09278321B2

    公开(公告)日：2016-03-08

    申请号：US11850229

    申请日：2007-09-05

    申请人： Gregory A. Dale ,  Ivor T. Knight

    发明人： Gregory A. Dale ,  Ivor T. Knight

    IPC分类号： B01F5/06 ,  B01L3/00 ,  B01F13/00 ,  B01L7/00

    CPC分类号： B01L3/502715 ,  B01F5/0602 ,  B01F5/0647 ,  B01F13/0059 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0829 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/049

    摘要： An assembly for performing micro-fluidic assays includes a micro-fluidic chip with access ports and micro-channels in communication with the access ports and a fluid cartridge having internal, fluid-containable chambers and a nozzle associated with each internal chamber that is configured to be coupled with an access port. Reaction fluids, such as sample material, buffer, and/or reagent, contained within the cartridge are dispensed from the cartridge into the access ports and micro-channels of the micro-fluidic chip. Embodiments of the invention include a cartridge which includes a waste compartment for receiving used DNA and other reaction fluids from the micro-channel at the conclusion of the assay.

    摘要翻译： 用于执行微流体测定的组件包括具有进入端口和与进入端口连通的微通道的微流体芯片和具有内部可流体容纳室的流体盒和与每个内部室相关联的喷嘴，其配置为 与访问端口耦合。 包含在药筒内的反应流体，例如样品材料，缓冲液和/或试剂从药筒分配到微流体芯片的进入口和微通道中。 本发明的实施例包括一个盒，其包括在分析结束时从微通道接收使用的DNA和其它反应流体的废物隔室。