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    Spatially-addressable immobilization of anti-ligands on surfaces
    7.
    发明授权
    Spatially-addressable immobilization of anti-ligands on surfaces 失效
    表面上抗配体的空间可寻址固定

    公开(公告)号：US5252743A

    公开(公告)日：1993-10-12

    申请号：US612671

    申请日：1990-11-13

    申请人： Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

    发明人： Ronald W. Barrett ,  Michael C. Pirrung ,  Lubert Stryer ,  Christopher P. Holmes ,  Steven A. Sundberg

    IPC分类号： C07D233/38 ,  C07D491/048 ,  C07D495/04 ,  C40B40/06 ,  G01N33/543 ,  G01N33/552 ,  C07D235/02

    CPC分类号： C07D495/04 ,  C07D233/38 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/552 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00659 ,  B01J2219/00722 ,  C40B40/06 ,  Y02P20/55

    摘要： Methods and compositions are described for immobilizing anti-ligands, such as antibodies or antigens, hormones or hormone receptors, oligonucleotides, and polysaccharides on surfaces of solid substrates for various uses. The methods provide surfaces covered with caged binding members which comprise protecting groups capable of being removed upon application of a suitable energy source. Spatially addressed irradiation of predefined regions on the surface permits immobilization of anti-ligands at the activated regions on the surface. Cycles of irradiation on different regions of the surface and immobilization of different anti-ligands allows formation of an immobilized matrix of anti-ligands at defined sites on the surface. The immobilized matrix of anti-ligands permits simultaneous screenings of a liquid sample for ligands having high affinities for certain anti-ligands of the matrix. A preferred embodiment of the invention involves attaching photoactivatable biotin derivatives to a surface. Photolytic activation of the biotin derivatives forms biotin analogs having strong binding affinity for avidin. Biotinylated anti-ligands can be immobilized on activated regions of the surface previously treated with avidin.

    摘要翻译： 描述了用于在固体底物的表面上固定抗配体如抗体或抗原，激素或激素受体，寡核苷酸和多糖的方法和组合物用于各种用途。 这些方法提供了覆盖有笼式结合构件的表面，其包括在施加合适的能量源时能够被去除的保护基团。 表面上预定区域的空间照射辐射允许在表面上的活化区域上固定抗配体。 表面不同区域的照射周期和不同抗配体的固定允许在表面上限定的位点形成固定的抗配体基团。 抗配体的固定化基质允许对基质的某些抗配体具有高亲和力的配体的液体样品的同时筛选。 本发明的优选实施方案涉及将可光活化的生物素衍生物连接到表面。 生物素衍生物的光解活化形成对抗生物素蛋白具有强结合亲和力的生物素类似物。 生物素化的抗配体可以固定在先前用抗生物素蛋白处理的表面的活化区上。

    High throughput microfluidic systems and methods
    10.
    发明授权
    High throughput microfluidic systems and methods 有权
    高通量微流体系统和方法

    公开(公告)号：US07316801B2

    公开(公告)日：2008-01-08

    申请号：US10244227

    申请日：2002-09-16

    申请人： Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

    发明人： Joseph E. Kercso ,  Steven A. Sundberg ,  Jeffrey A. Wolk ,  Andrew W. Toth ,  Calvin Y. H. Chow ,  J. Wallace Parce

    IPC分类号： G01N21/00 ,  B01L3/02

    CPC分类号： G01N35/028 ,  G01N2035/00148 ,  Y10T436/11 ,  Y10T436/113332 ,  Y10T436/119163 ,  Y10T436/25625 ,  Y10T436/2575

    摘要： The invention provides improved systems, devices, and methods for analyzing a large number of sample compounds contained in standard multi-well microtiter plates or other array structures. The multi-well plates travel along a conveyor system to a test station having a microfluidic device. At the test station, each plate is removed from the conveyor and the wells of the multi-well plate are sequentially aligned with an input port of the microfluidic device. After at least a portion of each sample has been input into the microfluidic channel system, the plate is returned to the conveyor system. Pre and/or post testing stations may be disposed along the conveyor system, and the use of an X-Y-Z robotic arm and novel plate support bracket allows each of the samples in the wells to be input into the microfluidic network through a probe affixed to a microfluidic chip. A clamshell structure having a hinged lid can releasably support the chip while providing and/or accommodating the electrical, optical, structural, and other interface connections between the microfluidic device and the surrounding system.

    摘要翻译： 本发明提供用于分析标准多孔微量滴定板或其它阵列结构中所含的大量样品化合物的改进的系统，装置和方法。 多孔板沿着输送系统行进到具有微流体装置的测试台。 在测试台处，每个板从输送机中取出，多孔板的孔依次与微流体装置的输入端对准。 在每个样品的至少一部分已经被输入到微流体通道系统中之后，板返回到输送系统。 可以沿输送机系统布置前后测试站，并且使用XYZ机器人臂和新的板支撑支架允许孔中的每个样品通过固定在微流体上的探针输入微流体网络 芯片。 具有铰接盖的蛤壳结构可以可释放地支撑芯片，同时提供和/或容纳微流体装置和周围系统之间的电，光学，结构和其它界面连接。