-
公开(公告)号：US20140110349A1
公开(公告)日：2014-04-24
申请号：US14118728
申请日：2012-04-13
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
CPC分类号： A61M1/34 , A61M1/3403 , B01D61/18 , B01D61/22 , B01D67/0032 , B01D71/50 , B01D2311/14 , G01N33/491 , G01N2001/4088
摘要： An assembly and a method for filtration are disclosured, which are suitable in particular for the filtration of cells, for example tumor cells, from a sample, for example a blood sample. In the method, a pressure differential between the pressure upstream and the pressure downstream of a filter is determined; and the pressure differential between upstream and downstream of the filter is adjusted such that the pressure differential does not exceed a predetermined value.
摘要翻译： 公开了组合物和过滤方法，其特别适用于从样品例如血液样品过滤细胞，例如肿瘤细胞。 在该方法中，确定过滤器下游压力和压力之间的压力差; 并且调节过滤器的上游和下游之间的压力差，使得压差不超过预定值。
-
2.发明申请公开(公告)号：US20120315664A1
公开(公告)日：2012-12-13
申请号：US13575651
申请日：2011-01-26
CPC分类号： G01N1/4077 , B01D63/08 , B01D69/10 , B01L3/502715 , B01L3/508 , B01L2200/027 , B01L2300/0681 , B01L2300/0822 , B01L2300/0864 , G01N2001/4088
摘要： An assembly and method are disclosed for the filtration of a liquid and the use thereof, wherein a supporting body is designed in a recess of a carrier and a filter membrane lies flat on the supporting body. The filter membrane and the supporting body are designed to be permeable to liquids and thus serve as filters, in particular for filtering tumor cells from blood. The carrier can having standard shapes of an object carrier for microscopy and the filtration residue on the filter membrane can be easily handled and examined in the microscope. As a result of the filter membrane lying level on the supporting body, the filtration residue can be particularly well examined microscopically.
摘要翻译： 公开了用于过滤液体的组件和方法及其用途，其中支撑体被设计在载体的凹部中，并且过滤膜平坦地位于支撑体上。 过滤膜和支撑体设计成可渗透液体，因此用作过滤器，特别是用于从血液中过滤肿瘤细胞。 载体可以具有用于显微镜的物体载体的标准形状，并且可以在显微镜中容易地处理和检查过滤膜上的过滤残余物。 作为过滤膜在支撑体上的位置的结果，过滤残留物可以在显微镜下进行特别良好的检查。
-
3.发明授权公开(公告)号：US09958435B2
公开(公告)日：2018-05-01
申请号：US14118723
申请日：2012-04-16
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
CPC分类号： G01N33/5091 , B01D63/08 , B01L3/508 , B01L2300/0681 , B01L2300/0822 , G01N1/2813 , G01N2001/045 , G01N2001/4088 , G01N2015/1006
摘要： A process is disclosed for detecting cells in a liquid sample, which includes: i) filtration of the liquid sample through a porous membrane which is suitable for retaining detectable cells, where at least one subregion of a support is configured as transparent supporting body and the membrane is arranged over its area on the transparent supporting body in such a way that detectable cells are retained on at least part of the surface of the membrane and that at least part of the sample liquid passes through the membrane, ii) application of a liquid optical medium which has essentially the same refractive index as the supporting body, and iii) optical measurement of at least a subarea of the membrane in order to detect detectable cells.
-
公开(公告)号：US20140106388A1
公开(公告)日：2014-04-17
申请号：US14118701
申请日：2012-04-13
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten Hiltawsky , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/50
CPC分类号： G01N33/5091 , C12Q1/6806 , G01N1/30
摘要： An embodiment relates to a method and to an array for detecting live circulating or disseminated cells in body fluids (for example blood, urine) or tissue samples (for example bone marrow) mixed with liquid. The method includes: a) filtering the liquid sample through a porous membrane that is suitable for retaining cells to be detected, such that cells to be detected come to rest upon at least a part of the surface of the membrane and the sample liquid passes the membrane; b) applying a first process liquid containing a first agent for marking the cells to be detected with a first marker; c) incubating the process liquid on the membrane for a predetermined time period, wherein the cells to be detected are marked; and d) detecting the marked cells to be detected on the surface of the membrane.
摘要翻译： 实施方案涉及用于检测与液体混合的体液（例如血液，尿液）或组织样品（例如骨髓）中的活的循环或播散细胞的方法和阵列。 该方法包括：a）通过适于保留待检测细胞的多孔膜过滤液体样品，使待检测细胞停留在膜表面的至少一部分上，样品液体通过 膜; b）施加含有用于用第一标记标记待检测细胞的第一试剂的第一处理液; c）将所述工艺液体在所述膜上孵育预定时间段，其中所述待检测的细胞被标记; 以及d）检测在膜的表面上待检测的标记细胞。
-
5.发明申请ARRANGEMENT AND PROCESS FOR OPTICAL ANALYSIS AND SPECIFIC ISOLATION OF BIOLOGICAL SAMPLES 有权光学分析和生物样品特异性分离的安排和方法公开(公告)号：US20140106355A1
公开(公告)日：2014-04-17
申请号：US14118723
申请日：2012-04-16
申请人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten HILtawsky , Peter Paulicka , Manfred Stanzel
发明人： Joachim Bangert , Katja Friedrich , Walter Gumbrecht , Karsten HILtawsky , Peter Paulicka , Manfred Stanzel
IPC分类号： G01N33/50
CPC分类号： G01N33/5091 , B01D63/08 , B01L3/508 , B01L2300/0681 , B01L2300/0822 , G01N1/2813 , G01N2001/045 , G01N2001/4088 , G01N2015/1006
摘要： A process is disclosed for detecting cells in a liquid sample, which includes: i) filtration of the liquid sample through a porous membrane which is suitable for retaining detectable cells, where at least one subregion of a support is configured as transparent supporting body and the membrane is arranged over its area on the transparent supporting body in such a way that detectable cells are retained on at least part of the surface of the membrane and that at least part of the sample liquid passes through the membrane, ii) application of a liquid optical medium which has essentially the same refractive index as the supporting body, and iii) optical measurement of at least a subarea of the membrane in order to detect detectable cells.
摘要翻译： 公开了一种用于检测液体样品中的细胞的方法，其包括：i）通过适于保持可检测细胞的多孔膜过滤液体样品，其中支撑体的至少一个子区域被配置为透明支撑体，并且 膜以透明支撑体上的区域布置，使得可检测的细胞保留在膜的至少部分表面上，并且至少部分样品液体通过膜，ii）施加液体 光学介质，其具有与支撑体基本相同的折射率，以及iii）光学测量膜的至少一个子区域以便检测可检测的细胞。
-
公开(公告)号：US10081831B2
公开(公告)日：2018-09-25
申请号：US12743316
申请日：2008-10-29
IPC分类号： C12Q1/68 , C12Q1/6837
CPC分类号： C12Q1/6837 , C12Q2527/107
摘要： A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p] Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)
-
7.发明申请VALVE FOR LAB-ON-A-CHIP SYSTEMS, METHOD FOR ACTUATING AND FOR PRODUCING VALVE 有权用于制动阀门的阀门，用于制动阀门的方法公开(公告)号：US20120090692A1
公开(公告)日：2012-04-19
申请号：US13375200
申请日：2010-04-29
CPC分类号： F16K99/0001 , B01L3/5027 , B01L2200/10 , B01L2300/0816 , B01L2300/0887 , B01L2400/0655 , F16K7/20 , F16K99/0026 , F16K2099/0078 , F16K2099/0084 , Y10T137/0396
摘要： A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.
摘要翻译： 实验室芯片系统的基板具有两个相邻的凹槽，一个用作流动通道，另一个用弹性体化合物填充。 在第一状态下，弹性体化合物和基底在一段中限定流动通道。 在第二状态下，弹性体化合物沿着流路的横截面占据基板的凹部的空间，从而完全封闭流路。 基材和弹性体化合物可以通过注塑技术制备。
-
公开(公告)号：US20100248243A1
公开(公告)日：2010-09-30
申请号：US12743316
申请日：2008-10-29
CPC分类号： C12Q1/6837 , C12Q2527/107
摘要： A method is disclosed for calibrating a sensor element, which has an immobilized probe oligonucleotide, via which the bonding of a target nucleic acid (Z) can be detected by the sensor. In at least one embodiment, the method includes: a) bringing the sensor element into contact with a control nucleic acid (K), the melting temperature Tm(K) of which is less than the melting temperature Tm(Z) of the target nucleic acid (Z); b) hybridizing the control nucleic acid (K) to the probe oligonucleotide at a temperature T[p] Tm(K) and detecting a negative control signal at a temperature T[n]. According to a refinement of at least one embodiment of the invention, a measuring signal of the target nucleic acid (Z) is measured at a measuring temperature T [mess], where Tm(K)
摘要翻译： 公开了一种用于校准具有固定化探针寡核苷酸的传感器元件的方法，通过该探针寡核苷酸可以通过传感器检测靶核酸（Z）的结合。 在至少一个实施方案中，该方法包括：a）使传感器元件与对照核酸（K）接触，其熔解温度Tm（K）小于靶核酸的解链温度Tm（Z） 酸（Z）; b）在温度T [p]
-
9.发明授权Device and method for controlling fluid flows in lab-on-a-chip systems and method for producing device 有权实验室芯片系统中控制流体流动的装置和方法及其制造方法公开(公告)号：US09151409B2
公开(公告)日：2015-10-06
申请号：US13375191
申请日：2010-04-29
IPC分类号： F16K11/065 , F16K31/524 , F16K99/00 , B01L3/00 , G01N35/10 , G01N35/00
CPC分类号： F16K11/065 , B01L3/502738 , B01L2300/0636 , B01L2300/0816 , B01L2300/123 , B01L2400/06 , B01L2400/0655 , B01L2400/0661 , B01L2400/082 , F16K7/16 , F16K11/161 , F16K27/003 , F16K31/52483 , F16K99/0001 , F16K99/0015 , F16K99/0042 , F16K2099/008 , F16K2099/0084 , G01N35/1097 , G01N2035/00158 , G01N2035/00237 , Y10T137/0396 , Y10T137/86445 , Y10T137/86919 , Y10T137/87
摘要： An array of valves are arranged in n columns and m lines and which are each designed to control a fluid flow in an associated flow channel in a lab-on-a-chip system. The array includes at least two valves, every column having not more than one valve and every line having from zero to n valves. A device is used for actuating the valves. The valves are pressure-actuated. To produce the device, the flow channels are arranged in accordance with the arrangement of the valves.
摘要翻译： 一组阀门以n列和m行排列，并且每个都被设计成控制在芯片实验室系统中相关联的流动通道中的流体流动。 该阵列包括至少两个阀，每列具有不超过一个阀，并且每一行具有从零到n的阀。 一个装置用于致动阀门。 阀是压力驱动的。 为了生产该装置，流动通道根据阀的布置被布置。
-
10.发明授权Valve for lab-on-a-chip systems, method for actuating and for producing valve 有权用于实验室芯片系统的阀门，用于致动和产生阀门的方法公开(公告)号：US09033307B2
公开(公告)日：2015-05-19
申请号：US13375200
申请日：2010-04-29
CPC分类号： F16K99/0001 , B01L3/5027 , B01L2200/10 , B01L2300/0816 , B01L2300/0887 , B01L2400/0655 , F16K7/20 , F16K99/0026 , F16K2099/0078 , F16K2099/0084 , Y10T137/0396
摘要： A substrate of a lab-on-a-chip system has two adjacent recesses, one serving as a flow channel and the other one being filled with an elastomer compound. In a first state, the elastomer compound and the substrate delimit the flow channel in a section. In a second state, the elastomer compound takes up the space in the recess in the substrate along a cross-section of the flow channel, thereby completely closing the flow channel. The substrate and the elastomer compound may be produced by injection molding techniques.
摘要翻译： 实验室芯片系统的基板具有两个相邻的凹槽，一个用作流动通道，另一个用弹性体化合物填充。 在第一状态下，弹性体化合物和基底在一段中限定流动通道。 在第二状态下，弹性体化合物沿着流路的横截面占据基板的凹部的空间，从而完全封闭流路。 基材和弹性体化合物可以通过注塑技术制备。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.019 秒)
