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公开(公告)号：US5234836A
公开(公告)日：1993-08-10
申请号：US841797
申请日：1992-02-26
申请人： Kiyoshi Nokihara , Koji Muramoto
发明人： Kiyoshi Nokihara , Koji Muramoto
CPC分类号： G01N33/6824
摘要： The present invention is directed to a method for amino acid sequence analysis comprising either the steps of labeling the sample with a fluorescent reagent and quenching the excess fluorescent reagent remaining after said labeling with an ammonium salt, or the steps of degrading amino acid from the amino terminus of peptides or proteins using a fluorescent Edman reagent and quenching the excess fluorescent Edman reagent remaining after said degrading with an ammonium salt. The method of the present invention makes it possible to eliminate the interference of identification by the chromatographic peak of fluorescent reagent by quenching the excess fluorescent reagent in the sample.
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公开(公告)号：US20110319347A1
公开(公告)日：2011-12-29
申请号：US13255551
申请日：2010-03-11
申请人： Kiyoshi Nokihara , Naeko Miyazato
发明人： Kiyoshi Nokihara , Naeko Miyazato
IPC分类号： A61K8/64 , C07K5/097 , A61Q19/02 , A61K38/07 , C07K5/117 , A61L2/00 , C07D213/68 , C07K5/087
CPC分类号： A61K31/4412 , A61K8/4926 , A61K8/64 , A61K38/00 , A61K2800/522 , A61Q19/02 , C07K5/0808 , C07K5/0812 , C07K5/0817 , C07K5/0821 , C07K5/0823 , C07K5/1024
摘要： Disclosed is an antioxidant which can exhibit a higher antioxidant ability than known antioxidative peptides. The antioxidant according to the present invention comprises mimosine and/or a mimosine-containing peptide(s). The mimosine-containing peptide is a peptide which comprises at least one mimosine residue in its amino acid sequence. Mimosine has stronger antioxidant potency than known antioxidative tripeptides even when used as it is alone, and therefore is useful as an antioxidant. In addition, the antioxidant action of a peptide can be increased by introducing mimosine thereinto, thereby obtaining a peptide with higher antioxidant potency than the original one.
摘要翻译： 公开了一种抗氧化剂，其可以表现出比已知的抗氧化肽更高的抗氧化能力。 根据本发明的抗氧化剂包含含有肌动蛋白和/或含有含有嗜好肽的肽。 含有含mimosine的肽是在其氨基酸序列中包含至少一个mimosine残基的肽。 即使在单独使用时，肌酐具有比已知的抗氧化三肽更强的抗氧化能力，因此可用作抗氧化剂。 此外，通过在其中引入含有肌动蛋白的方法可以增加肽的抗氧化作用，从而获得比原来更高的抗氧化剂效能的肽。
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公开(公告)号：US20100130380A1
公开(公告)日：2010-05-27
申请号：US11922108
申请日：2006-06-16
申请人： Kiyoshi Nokihara , Yasuo Oka
发明人： Kiyoshi Nokihara , Yasuo Oka
CPC分类号： G01N33/54393 , B01L3/5085 , B01L2200/12 , B01L2300/0636 , B01L2300/0829 , B01L2300/0893 , B01L2300/0896 , B01L2300/12 , B82Y30/00 , C40B60/14
摘要： A biochip substrate which is free from cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same. A biochip substrate on which multiple valleys for immobilizing biological substances are formed so as to prevent cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same are provided. Moreover, it is found out that a desired binding in a target molecule contained in a test sample occurs at a detectable level in a solution system even in the case where a valley have such a small capacity as 1 nL to 10 nL.
摘要翻译： 一种生物芯片基板，其由于点扩散或与彼此相邻的点接触而没有交叉污染，并且使用该生物芯片。 其上形成有用于固定生物物质的多个谷的生物芯片基板，以防止由于点扩散或彼此相邻的点的接触而产生的交叉污染，并且提供了使用该生物体的生物芯片。 此外，发现测试样品中包含的靶分子中期望的结合在溶液体系中发生在可检测的水平，即使在谷具有如1nL至10nL这样小的容量的情况下也是如此。
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公开(公告)号：US5442122A
公开(公告)日：1995-08-15
申请号：US145101
申请日：1993-11-03
申请人： Masaki Noda , Kiyoshi Nokihara
发明人： Masaki Noda , Kiyoshi Nokihara
IPC分类号： C07C235/08 , C07C217/74 , C07C235/12 , C07C237/08 , C07C237/12 , C07C237/22 , C07C271/24 , C07K1/04 , C07C211/00
CPC分类号： C07C271/24 , C07C217/74 , C07K1/042 , C07C2103/18 , C07C2103/32 , Y10S530/816
摘要： The present invention is directed to a compound represented by the following formula (I), ##STR1## and a linker for peptide synthesis using the above compound. When the linker of the present invention is used for the solid-phase peptide synthesis, it is possible to synthesize those peptides which are sensitive to acid and difficult to synthesize by conventional methods. Also, side reactions can be prevented, and the desired product is produced at a high purity because cleavage can be achieved under milder conditions or in shorter times. In other words, efficient peptide synthesis is possible.
摘要翻译： 本发明涉及由下式（I）表示的化合物，（I）和使用上述化合物的肽合成接头。 当本发明的接头用于固相肽合成时，可以通过常规方法合成那些对酸敏感并难以合成的那些肽。 此外，可以防止副反应，并且所需产物以高纯度制备，因为在较温和的条件下或在较短时间内可以实现切割。 换句话说，有效的肽合成是可能的。
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公开(公告)号：US5344613A
公开(公告)日：1994-09-06
申请号：US9080
申请日：1993-01-26
CPC分类号： B01L99/00 , B01J19/0046 , C07K1/045 , B01J2219/00286 , B01J2219/00288 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/00725 , C40B40/10 , C40B60/14
摘要： In an automated solid-phase peptide synthesizing apparatus comprising at least one reaction vessel containing particulate resin as an insoluble support matrix which anchors elongating peptide chains yielded through coupling-assembly formation of peptide bonds, mixing agitation of reagents, as well as circulation agitation of washing solvents flushing the coupling-associated reaction solutions, introduced in peptide chain-assembly process steps into the reaction vessel, is effected by the bubbling of an inert gas forcibly passed through the reagents or washing solvent via a drainage port of the reaction vessel, in order to promote peptide synthesis. A removable agitation stabilizer is inserted into the reaction vessel as a barrier for inhibiting extra-vessel escape, due to action of the bubbling inert gas, of the reagents as well as the washing solvents, and of the support matrix anchoring the elongating peptide chains. The stabilizer also breaks clumping and coagulative skinning of elongated peptides bound to the support matrix, as effects further consequent of the action of the bubbling inert gas and levitated thereby toward the mouth of the reaction vessel during peptide synthesis. The stabilizer additionally acts as a baffle furthering mixing agitation, to promote dispersive mixing of the peptidyl resin with the reagents and washing solvents during peptide chain assembly; and It also serves to distribute washing solvents evenly along the inner surface of the reaction vessel.
摘要翻译： 在一种自动化固相肽合成装置中，包括至少一个含有颗粒状树脂作为不溶性支持基质的反应容器，该反应容器通过肽键的偶联 - 组装形成固定延伸的肽链，混合搅拌试剂以及循环搅拌洗涤 将通过肽链组装工艺步骤引入反应容器的偶联相关反应溶液冲洗到反应容器中，通过以反应容器的排水口强制通过试剂或洗涤溶剂的惰性气体起泡 促进肽合成。 将可移除的搅拌稳定剂插入到反应容器中，作为阻止由于起泡惰性气体的作用以及洗涤溶剂和固定伸长肽链的载体基质而导致的外部容器逸出的屏障。 稳定剂还破坏结合到载体基质上的细长肽的结块和凝结性皮肤剥离，其作用是由于气泡惰性气体的作用而进一步引起，并且由此在肽合成期间悬浮于反应容器口中。 稳定剂另外作为进一步混合搅拌的挡板，以促进肽链组装期间肽基树脂与试剂和洗涤溶剂的分散混合; 并且还用于沿着反应容器的内表面均匀分布洗涤溶剂。
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公开(公告)号：US09778256B2
公开(公告)日：2017-10-03
申请号：US11922108
申请日：2006-06-16
申请人： Kiyoshi Nokihara , Yasuo Oka
发明人： Kiyoshi Nokihara , Yasuo Oka
IPC分类号： C40B60/14 , G01N33/543 , B01L3/00 , B82Y30/00
CPC分类号： G01N33/54393 , B01L3/5085 , B01L2200/12 , B01L2300/0636 , B01L2300/0829 , B01L2300/0893 , B01L2300/0896 , B01L2300/12 , B82Y30/00 , C40B60/14
摘要： A biochip substrate which is free from cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same. A biochip substrate on which multiple valleys for immobilizing biological substances are formed so as to prevent cross-contamination due to spot spreading or contact with spots adjacent to each other, and a biochip using the same are provided. Moreover, it is found out that a desired binding in a target molecule contained in a test sample occurs at a detectable level in a solution system even in the case where a valley have such a small capacity as 1 nL to 10 nL.
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公开(公告)号：US20100184622A1
公开(公告)日：2010-07-22
申请号：US11915366
申请日：2005-05-24
申请人： Kiyoshi Nokihara , Yasuo Oka
发明人： Kiyoshi Nokihara , Yasuo Oka
CPC分类号： G01N33/553 , B01J19/0046 , B01J2219/00387 , B01J2219/00497 , B01J2219/00527 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , G01N33/54353 , G01N33/551 , Y10T428/31678
摘要： A substrate for biochips with which immobilization is easy, which does not exhibit self-fluorescence, which is easy to manufacture, and which is excellent in flatness and surface precision, is disclosed. A substrate having a substrate body of the biochip, which is made of a metal, and a carbon layer having functional groups formed on the metal substrate body is used as a substrate for biochips. Since the substrate body of the substrate for biochips is made of a metal, the substrate is not only easy to manufacture, but also free from cracking and chipping, so that it allows easy handling, and high flatness and surface precision can be attained. Therefore, the problem that the optical system is hard to focus when detecting fluorescence does not occur. Moreover, since the substrate body is made of a metal, it does not emit fluorescence by itself. In addition, since the carbon layer has functional groups such as amino groups, biologically relevant substances can be easily immobilized.
摘要翻译： 公开了一种容易制造的生物芯片用基板，其固定化容易，不易发生自发荧光，平坦度和表面精度优异。 使用具有由金属制成的生物芯片的基板主体和在金属基板主体上形成有官能团的碳层的基板作为生物芯片的基板。 由于用于生物芯片的基板的基板主体由金属制成，所以基板不仅容易制造，而且没有破裂和碎裂，从而易于处理，并且可以获得高平坦度和表面精度。 因此，不发生检测荧光时光学系统难以聚焦的问题。 此外，由于基板主体由金属制成，它本身不会发出荧光。 此外，由于碳层具有诸如氨基的官能团，因此可以容易地固定生物相关的物质。
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公开(公告)号：US20100178222A1
公开(公告)日：2010-07-15
申请号：US12664621
申请日：2008-06-13
申请人： Kiyoshi Nokihara , Yasuo Oka
发明人： Kiyoshi Nokihara , Yasuo Oka
IPC分类号： B01J19/00
CPC分类号： B01J19/0093 , B01J2219/00783 , B01J2219/00819 , B01J2219/00831 , B01J2219/0086 , B01J2219/00873 , B01L3/502707 , B01L2200/0689 , B01L2300/0883 , B01L2300/12 , B81B2201/051 , B81B2201/058 , B81B2203/0307 , B81C3/001 , B81C2203/0109 , B81C2203/032
摘要： Means for overcoming the problems which the conventional glass microchannel chips have are disclosed. That is, a microchannel chip in which microchannels can be formed at a low cost, and which has a high chemical resistance is disclosed. The microchannel chip is constituted by a substrate made of carbon, which has a channel in its surface; and a cover composed of a glass plate bonded to the substrate. The cover is bonded to the substrate by heating at least a part of the contact surface at which the substrate is in contact with the cover.
摘要翻译： 公开了克服传统玻璃微通道芯片所存在的问题的手段。 也就是说，公开了一种微通道芯片，其中可以以低成本形成微通道，并且具有高耐化学性。 微通道芯片由碳表面构成，其表面具有通道; 以及由与基板结合的玻璃板构成的盖。 通过加热基板与盖接触的接触表面的至少一部分，将盖结合到基板。
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公开(公告)号：US20050084902A1
公开(公告)日：2005-04-21
申请号：US10476861
申请日：2002-05-07
申请人： Kiyoshi Nokihara , Hisakazu Mihara
发明人： Kiyoshi Nokihara , Hisakazu Mihara
CPC分类号： C40B30/04 , C07K1/1077 , C07K14/00 , G01N33/6845
摘要： Disclosed is a peptide-immobilized substrate for measuring a target protein, with which the peptide can have a structure required for being recognized by the target protein, with which the accurate loading amount of the peptide can be attained, and by which a trace amount of the target protein may be measured accurately and simply. The peptide-immobilized substrate for measuring a target protein according to the present invention comprises a chemically synthesized peptide having an. expected spatial structure or having a binding ability with the target protein, which peptide can bind with the target protein and is immobilized on the substrate.
摘要翻译： 公开了用于测定靶蛋白质的肽固定基质，肽可以具有被靶蛋白识别所需的结构，可以获得肽的精确负载量，并且通过其 可以准确且简单地测量靶蛋白。 根据本发明的用于测量靶蛋白质的固定肽的底物包含化学合成的肽。 预期的空间结构或具有与靶蛋白的结合能力，该肽可与靶蛋白结合并固定在底物上。
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公开(公告)号：US5356596A
公开(公告)日：1994-10-18
申请号：US22036
申请日：1993-02-24
CPC分类号： C07K1/045 , B01J19/0046 , B01J2219/00286 , B01J2219/00288 , B01J2219/00308 , B01J2219/00454 , B01J2219/005 , B01J2219/00725 , C40B40/10 , C40B50/14 , C40B60/14
摘要： In connection with an automated peptide synthesizer, an apparatus and associated method through which peptides produced in simultaneous multiple synthesis are together isolable. Cleavage to liberate the peptides from linkage to the support matrix of the solid phase is carried out in the same vessels in which the synthesizing reactions took place. Prior to cleavage, a drainage port of each reaction vessel is closed off by a cap. The reaction vessel is inserted into a centrifuge tube, which in turn is put into a rack supporting a number of tubes equal to the number of channels of the peptide synthesizing apparatus. After the peptides are cleaved and dissolved into cleaving solution added into each of the reaction vessels, the caps are removed from the drainage ports of each reaction vessel, and the vessel is returned into the centrifuge tube. A plastic jet-fitting attached to the nozzle tip of a blow unit pressure gun is inserted into and pressed into contact against the supply opening of the reaction vessel. Operating a trigger of the pressure gun releases pressurized inert gas into the reaction chamber. The peptide-dissolved cleaving solution is thereby passed through the drainage port, and is thus transferred in liquid phase into the centrifuge tube as filtrate. Associated with the blow unit pressure gun, a needle tube fitting is provided for localized desiccation of wetted peptide following precipitation and centrifuging.
摘要翻译： 关于自动肽合成仪，同时多重合成中产生的肽一起可分离的装置和相关方法。 在与发生合成反应的相同的容器中进行从固定相的支持基质的连接释放肽的切割。 在切割之前，每个反应容器的排水口由盖封闭。 将反应容器插入到离心管中，该离心管又被放入支架中，该支架中的多个管等于肽合成装置的通道数。 在肽被切割并溶解到每个反应容器中的裂解溶液中之后，从每个反应容器的排出口移除盖子，并将容器返回到离心管中。 将附接到吹塑单元压力枪的喷嘴尖端的塑料喷射管插入并压接到反应容器的供应开口。 操作压力枪的触发器将加压的惰性气体释放到反应室中。 因此，肽溶解的解离溶液通过排出口，因此以液相方式转移到作为滤液的离心管中。 与吹气单元压力枪相关联，提供针管配件，用于沉淀和离心后的湿肽的局部干燥。
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