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公开(公告)号：US20050129581A1
公开(公告)日：2005-06-16
申请号：US10818642
申请日：2004-04-05
申请人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
发明人： Lincoln McBride , Marc Unger , Michael Lucero , Hany Nassef , Geoffrey Facer
CPC分类号： B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US20050019792A1
公开(公告)日：2005-01-27
申请号：US10819088
申请日：2004-04-05
申请人： Lincoln McBride , Michael Lucero , Marc Unger , Hany Nassef , Geoffrey Facer
发明人： Lincoln McBride , Michael Lucero , Marc Unger , Hany Nassef , Geoffrey Facer
CPC分类号： B01L3/00 , B01F13/0059 , B01J2219/00337 , B01J2219/00353 , B01J2219/00403 , B01J2219/00495 , B01J2219/00585 , B01J2219/0059 , B01J2219/0072 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/50851 , B01L2300/0627 , B01L2300/0883 , B01L2300/123 , B01L2400/06 , C12Q1/6844 , C40B60/08 , C40B60/14 , G01N35/00029 , G01N35/0098 , G01N2035/00366 , Y10T436/25 , C12Q2565/501
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US20110189678A1
公开(公告)日：2011-08-04
申请号：US12859176
申请日：2010-08-18
CPC分类号： B01L3/00 , B01F13/0059 , B01J2219/00337 , B01J2219/00353 , B01J2219/00403 , B01J2219/00495 , B01J2219/00585 , B01J2219/0059 , B01J2219/0072 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/50851 , B01L2300/0627 , B01L2300/0883 , B01L2300/123 , B01L2400/06 , C12Q1/6844 , C40B60/08 , C40B60/14 , G01N35/00029 , G01N35/0098 , G01N2035/00366 , Y10T436/25 , C12Q2565/501
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
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公开(公告)号：US07749737B2
公开(公告)日：2010-07-06
申请号：US11929436
申请日：2007-10-30
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M次.N基质微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US20130171633A1
公开(公告)日：2013-07-04
申请号：US13548068
申请日：2012-07-12
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US20080230387A1
公开(公告)日：2008-09-25
申请号：US11929625
申请日：2007-10-30
IPC分类号： G01N27/26
CPC分类号： B01L3/00 , B01L3/5027 , B01L3/502723 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/142 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/123 , B01L2400/0481 , B01L2400/0638 , C12Q1/686
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US09150913B2
公开(公告)日：2015-10-06
申请号：US13548068
申请日：2012-07-12
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M×N矩阵微流体装置，所述装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US08247178B2
公开(公告)日：2012-08-21
申请号：US12579347
申请日：2009-10-14
CPC分类号： C12Q1/68 , B01L3/0248 , B01L3/5025 , B01L3/5027 , B01L3/502707 , B01L3/502715 , B01L3/50273 , B01L3/502738 , B01L7/52 , B01L2200/0642 , B01L2200/10 , B01L2200/142 , B01L2200/147 , B01L2300/06 , B01L2300/0636 , B01L2300/0816 , B01L2300/0819 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/0874 , B01L2300/123 , B01L2300/1805 , B01L2400/0481 , B01L2400/0487 , B01L2400/06 , B01L2400/0638 , B01L2400/0655 , C12Q1/686 , G01N27/453
摘要： An M×N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
摘要翻译： 一种用于执行反应矩阵的M×N矩阵微流体装置，该装置具有多个反应池，其通过形成在装置的弹性体块内的通孔与样品入口或试剂入口之一连通。 提供的方法包括在微流体装置的弹性体块的弹性体层中平行形成通孔的方法，该方法包括使用图案化的光致抗蚀剂掩模和蚀刻试剂来蚀刻掉弹性体块的弹性体层的区域或部分。
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公开(公告)号：US07837946B2
公开(公告)日：2010-11-23
申请号：US12291319
申请日：2008-11-06
IPC分类号： B01L3/00
CPC分类号： B01L3/00 , B01F13/0059 , B01J2219/00337 , B01J2219/00353 , B01J2219/00403 , B01J2219/00495 , B01J2219/00585 , B01J2219/0059 , B01J2219/0072 , B01L3/502707 , B01L3/502715 , B01L3/502738 , B01L3/50851 , B01L2300/0627 , B01L2300/0883 , B01L2300/123 , B01L2400/06 , C12Q1/6844 , C40B60/08 , C40B60/14 , G01N35/00029 , G01N35/0098 , G01N2035/00366 , Y10T436/25 , C12Q2565/501
摘要： A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyzes. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyzes requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyzes.
摘要翻译： 提供了各种基于弹性体的微流体装置和用于使用和制造这种装置的方法。 某些设备具有反应位置阵列以便于高通量分析。 一些装置还包括位于盲制通道末端的反应位置，在制造过程中试剂已经预先沉积。 一旦将样品引入反应部位，试剂就会悬浮。 该装置可以与各种加热装置一起使用，因此可用于需要温度控制的各种分析，包括热循环应用，如核酸扩增反应，基因分型和基因表达分析。
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公开(公告)号：US08828663B2
公开(公告)日：2014-09-09
申请号：US11912502
申请日：2006-03-20
IPC分类号： C12Q1/68 , C12P19/34 , G01N27/453 , B01L3/00
CPC分类号： C12Q1/686 , B01L3/502738 , B01L7/52 , B01L2200/147 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/0887 , B01L2300/0893 , B01L2300/123 , B01L2300/1805 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , G01N27/453 , C12Q2565/629
摘要： Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.
摘要翻译： 用于执行样品中靶标相对浓度的装置和方法，含有靶和非目标成分的样品，通过将样品分成大量反应体积进行的方法，使得靶相对于非目标成分集中 并且在每个反应体积上进行检测测定以检测目标。
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