公开(公告)号：US5863784A

公开(公告)日：1999-01-26

申请号：US859032

申请日：1997-05-20

申请人： Naohiro Yoshigi ,  Hideo Maeba ,  Yukio Okada

发明人： Naohiro Yoshigi ,  Hideo Maeba ,  Yukio Okada

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/26 ,  C12N15/56 ,  C12R1/19 ,  C12N9/24 ,  C08B30/04 ,  C12N15/00 ,  C12P19/22

CPC分类号： C12N9/2425

摘要： A recombinant .beta.-amylase which is superior to the original recombinant .beta.-amylase in thermostability has been obtained by a site-directed mutagenesis with the recombinant .beta.-amylase gene coding 531 amino acid residues. Substitutions were MET.sub.181 of said enzyme with Leu, Ser.sub.291 with Ala, Ile.sub.293 with Val, Ser.sub.346 with Pro, Ser.sub.347 with Pro, Gln.sub.348, with Asp and Ala.sub.372 with Ser.

摘要翻译： 通过用编码531个氨基酸残基的重组β-淀粉酶基因进行定点诱变，获得了优于原始重组β-淀粉酶的重组β-淀粉酶。 取代为所述酶为Leu，Ser291与Ala，Ile293与Val，Ser346与Pro，Ser347与Pro，Gln348，Asp和Ala372与Ser的所述酶的MET181。

公开(公告)号：US5688684A

公开(公告)日：1997-11-18

申请号：US531601

申请日：1995-09-21

申请人： Naohiro Yoshigi ,  Hideo Maeba ,  Yukio Okada

发明人： Naohiro Yoshigi ,  Hideo Maeba ,  Yukio Okada

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N1/21 ,  C12N9/26 ,  C12N15/56 ,  C12R1/19 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/2425

摘要： A recombinant .beta.-amylase which is superior to the original recombinant .beta.-amylase in thermostability has been obtained by a site-directed mutagenesis with the recombinant .beta.-amylase gene coding 531 amino acid residues. Substitutions were MET.sub.181 of said enzyme with Leu, Ser.sub.291 with Ala, Ile.sub.293 with Val, Ser.sub.346 with Pro, Ser.sub.347 with Pro, Gln.sub.348 with Asp and Ala.sub.372 with Ser.

摘要翻译： 通过用编码531个氨基酸残基的重组β-淀粉酶基因进行定点诱变，获得了优于原始重组β-淀粉酶的重组β-淀粉酶。 取代为所述酶为Leu，Ser291与Ala，Ile293与Val，Ser346与Pro，Ser347与Pro，Gln348与Asp和Ala372与Ser的所述酶的MET181。

公开(公告)号：US5952489A

公开(公告)日：1999-09-14

申请号：US793599

申请日：1997-03-04

申请人： Yukio Okada ,  Naohiro Yoshigi ,  Kazutoshi Ito ,  Makoto Kihara

发明人： Yukio Okada ,  Naohiro Yoshigi ,  Kazutoshi Ito ,  Makoto Kihara

IPC分类号： C07K14/415 ,  C12N9/26 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  C12N15/29

CPC分类号： C12N9/2422 ,  C07K14/415 ,  C12N15/8234 ,  C12N9/2425

摘要： The invention provides a promoter capable of expressing an introduced gene in plant seeds, a vector comprising said promoter, a method for producing transgenic plants through transformation of plants with said vector, and a transgenic plant as transformed with said vector.After a suitable foreign gene and a terminator are linked to the promoter, the resulting vector may be introduced into seeds of barley or other plants, thereby intentionally modifying the seeds and making the resulting seeds produce foreign substances therein.

摘要翻译： PCT No.PCT / JP96 / 01866 Sec。 371日期1997年3月4日 102（e）1997年3月4日PCT PCT 1996年7月5日PCT公布。 公开号WO97 / 02353 日期1997年1月23日本发明提供能够在植物种子中表达引入基因的启动子，包含所述启动子的载体，通过用所述载体转化植物产生转基因植物的方法，以及用所述载体转化的转基因植物。 在合适的外源基因和终止子与启动子连接之后，所得到的载体可以被引入大麦或其他植物的种子中，从而有意地修饰种子并使得到的种子在其中产生异物。

公开(公告)号：US5726057A

公开(公告)日：1998-03-10

申请号：US737597

申请日：1996-11-25

申请人： Naohiro Yoshigi ,  Yukio Okada

发明人： Naohiro Yoshigi ,  Yukio Okada

IPC分类号： C12N15/09 ,  C07H21/04 ,  C07K14/415 ,  C12N9/26 ,  C12N15/56 ,  C12N15/63

CPC分类号： C12N9/2425

摘要： The present invention provides a barley .beta.-amylase structural gene consisting of the nucleotide sequence shown in SEQ. ID. No. 1 in attached sequence listing, a barley .beta.-amylase structural gene consisting of the nucleotide sequence shown in SEQ. ID. No. 2 in attached sequence listing, and a plasmid containing the nucleotide sequence shown in SEQ. ID. NO. 1 or 2 in attached sequence listing. The present invention can be utilized in breeding of barley and other plants and industrial production of .beta.-amylase and can contribute in the technical fields of plant breeding, production of brewed products such as beer, production of distilled liquors, food production and enzyme industry.

摘要翻译： PCT No.PCT / JP96 / 00799 Sec。 371日期：1996年11月25日 102（e）日期1996年11月25日PCT 1996年3月27日PCT公布。 第WO96 / 30525号公报 日期：1996年10月3日本发明提供了由SEQ ID NO：1所示的核苷酸序列组成的大麦β-淀粉酶结构基因。 ID。 附着序列表中的第1号，由SEQ ID NO：1所示的核苷酸序列组成的大麦β-淀粉酶结构基因。 ID。 连接序列表中的第2号和含有SEQ ID NO：1所示的核苷酸序列的质粒。 ID。 没有。 1或2在附加的序列表中。 本发明可用于大麦和其他植物的育种和β-淀粉酶的工业化生产，并且可以在植物育种，酿造啤酒生产，蒸馏酒生产，食品生产和酶工业的技术领域中有所贡献。

公开(公告)号：US20130220073A1

公开(公告)日：2013-08-29

申请号：US13824249

申请日：2011-08-22

申请人： Masashi Suto ,  Yuichi Takamura ,  Masaki Mita ,  Hiroyuki Uchimura ,  Isao Abe ,  Makoto Ogiwara ,  Naruhito Ogino ,  Yukio Okada ,  Junichi Sekiguchi

发明人： Masashi Suto ,  Yuichi Takamura ,  Masaki Mita ,  Hiroyuki Uchimura ,  Isao Abe ,  Makoto Ogiwara ,  Naruhito Ogino ,  Yukio Okada ,  Junichi Sekiguchi

IPC分类号： F16H57/029 ,  B29C70/00

CPC分类号： F16H57/029 ,  B29C70/00 ,  F16H1/16 ,  F16H57/031 ,  F16H57/039 ,  F16H2057/02082 ,  H02K5/10 ,  H02K7/1166 ,  Y10T74/2186

摘要： A seal member is attached to a cover which occludes a gear case as a housing unit. The seal member is formed of elastomer and integrally formed with: a plate-shaped portion formed into a disc shape which covers the inner surface of the cover; an inner seal portion which is positioned on the inner peripheral edge of a through-hole, and comes into sliding contact with the outer peripheral surface of an output member; an outer seal portion which is positioned on the periphery of the cover, and comes into contact with the opening end of the housing unit; and anchor portions which latch onto the front surface of the cover via mounting holes provided to the cover. The boundary between the seal member and the cover extends only to the outside of the gear case, and does not extend to the inside of the gear case.

摘要翻译： 密封构件附接到封闭作为壳体单元的齿轮箱的盖。 密封部件由弹性体形成，一体地形成有：形成为覆盖盖的内表面的盘状的板状部分; 内密封部，其位于通孔的内周缘部，与输出部件的外周面滑动接触; 外密封部，其位于所述盖的周围，并与所述壳体单元的开口端接触; 以及通过设置在所述盖上的安装孔将所述固定部分锁定在所述盖的前表面上。 密封构件和盖之间的边界仅延伸到齿轮箱的外部，并且不延伸到齿轮箱的内部。

公开(公告)号：US20100143909A1

公开(公告)日：2010-06-10

申请号：US12516960

申请日：2007-11-26

申请人： Yukio Okada ,  Yutaka Itoga

发明人： Yukio Okada ,  Yutaka Itoga

IPC分类号： C12Q1/68

CPC分类号： C12Q1/701 ,  C12Q2531/143 ,  C12Q2531/113 ,  C12Q2525/301

摘要： The invention provides a detection method for apple mosaic virus that comprises an extraction step in which apple mosaic virus RNA is extracted from a specimen, an amplification step in which a primer set containing 4 different oligonucleotides consisting of the nucleotide sequences represented by SEQ ID NO: 1-4 of the Sequence Listing is used for amplification of cDNA by RT-LAMP using the RNA as template, and a judging step in which apple mosaic virus is judged to be present when amplification of cDNA containing the nucleotide sequence represented by SEQ ID NO: 8 of the Sequence Listing has occurred in the amplification step.

摘要翻译： 本发明提供了一种苹果花叶病毒的检测方法，其包括提取步骤，其中从样品中提取苹果花叶病毒RNA，扩增步骤，其中含有由SEQ ID NO：1所示的核苷酸序列组成的4种不同寡核苷酸的引物组， 1-4的序列用于通过使用RNA作为模板的RT-LAMP扩增cDNA，以及判断步骤，其中当扩增含有SEQ ID NO所示的核苷酸序列的cDNA时，判定苹果花叶病毒存在 ：序列表中的8发生在扩增步骤中。

公开(公告)号：US07060815B2

公开(公告)日：2006-06-13

申请号：US10390658

申请日：2003-03-19

申请人： Yukio Okada ,  Kazutoshi Ito

发明人： Yukio Okada ,  Kazutoshi Ito

IPC分类号： C07H21/04 ,  C12N5/04 ,  C12N15/82 ,  C12N15/87 ,  A01H5/00

CPC分类号： C12N9/1037 ,  C12N15/8222 ,  C12N15/8233 ,  C12N15/8243

摘要： An isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. Hops are dioecious, and only female plants bear cones, the lupulin glands of which contain secondary metabolic products which provide bitterness and flavor to beer. These secondary metabolic products contain some pharmacologically effective compounds. In order to breed a more useful cultivar of hops by manipulating the constituent of such useful secondary metabolic products relying on genetic engineering techniques, this invention provides an isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. By using this nucleic acid, it is possible to develop a novel method for breeding hops with transformation techniques and molecular selection techniques. Furthermore, the present invention also provides a nucleic acid comprising the regulatory region for specifically expressing genes in lupulin glands. This nucleic acid can be used also for hop breeding.

公开(公告)号：US06864121B2

公开(公告)日：2005-03-08

申请号：US09970013

申请日：2001-10-02

申请人： Noriaki Sakamoto ,  Yoshiyuki Kobayashi ,  Junji Sakamoto ,  Yukio Okada ,  Yusuke Igarashi ,  Eiju Maehara ,  Kouji Takahashi

发明人： Noriaki Sakamoto ,  Yoshiyuki Kobayashi ,  Junji Sakamoto ,  Yukio Okada ,  Yusuke Igarashi ,  Eiju Maehara ,  Kouji Takahashi

IPC分类号： H01L21/48 ,  H01L21/56 ,  H01L21/68 ,  H01L23/31 ,  H05K3/00 ,  H05K3/06 ,  H05K3/20 ,  H01L21/44

CPC分类号： H01L21/568 ,  H01L21/4857 ,  H01L21/561 ,  H01L21/6835 ,  H01L23/3121 ,  H01L24/97 ,  H01L2221/68345 ,  H01L2221/68377 ,  H01L2224/05568 ,  H01L2224/05573 ,  H01L2224/16 ,  H01L2224/48091 ,  H01L2224/48227 ,  H01L2224/48464 ,  H01L2224/73265 ,  H01L2224/85001 ,  H01L2224/97 ,  H01L2924/00014 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01024 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01047 ,  H01L2924/01059 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/09701 ,  H01L2924/12041 ,  H01L2924/12042 ,  H01L2924/13055 ,  H01L2924/14 ,  H01L2924/15311 ,  H01L2924/15787 ,  H01L2924/181 ,  H01L2924/19041 ,  H01L2924/19043 ,  H01L2924/19105 ,  H05K3/0058 ,  H05K3/06 ,  H05K3/20 ,  H01L2224/85 ,  H01L2224/83 ,  H01L2924/00 ,  H01L2224/05599 ,  H01L2924/00012

摘要： A conductive pattern of a first layer isolated by an isolation trench is formed on a conductive foil, and a plurality of layers of the conductive patterns are formed thereon to create a multilayered wiring structure, and furthermore, a circuit element is mounted and molded with an insulating resin and the back surface of the conductive foil is etched. It is possible to implement a method of manufacturing a circuit device which provides very power saving and is suitable for mass production, then the circuit device having conductive patterns of a multilayered structure are provided.

摘要翻译： 在导电箔上形成由隔离沟槽隔离的第一层的导电图案，并且在其上形成多层导电图案以形成多层布线结构，此外，电路元件安装和模制 绝缘树脂和导电箔的背面蚀刻。 可以实现提供非常省电并且适合于批量生产的电路装置的制造方法，然后提供具有多层结构的导电图案的电路装置。

公开(公告)号：US06706547B2

公开(公告)日：2004-03-16

申请号：US10010890

申请日：2001-12-06

申请人： Noriaki Sakamoto ,  Yoshiyuki Kobayashi ,  Junji Sakamoto ,  Yukio Okada ,  Yusuke Igarashi ,  Eiju Maehara ,  Kouji Takahashi

发明人： Noriaki Sakamoto ,  Yoshiyuki Kobayashi ,  Junji Sakamoto ,  Yukio Okada ,  Yusuke Igarashi ,  Eiju Maehara ,  Kouji Takahashi

IPC分类号： H01L2100

CPC分类号： H05K1/188 ,  H01L21/4821 ,  H01L21/4832 ,  H01L23/3107 ,  H01L24/45 ,  H01L24/48 ,  H01L24/73 ,  H01L24/97 ,  H01L2221/68377 ,  H01L2224/05599 ,  H01L2224/32245 ,  H01L2224/45144 ,  H01L2224/48091 ,  H01L2224/48247 ,  H01L2224/48464 ,  H01L2224/73265 ,  H01L2224/85399 ,  H01L2224/97 ,  H01L2924/00014 ,  H01L2924/01013 ,  H01L2924/01024 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01059 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/07802 ,  H01L2924/09701 ,  H01L2924/12041 ,  H01L2924/12042 ,  H01L2924/13055 ,  H01L2924/14 ,  H01L2924/15787 ,  H01L2924/181 ,  H01L2924/19041 ,  H01L2924/19043 ,  H01L2924/19105 ,  H01L2924/19106 ,  H05K3/06 ,  Y10T29/49117 ,  Y10T29/49144 ,  Y10T29/49146 ,  Y10T29/49155 ,  Y10T29/49171 ,  Y10T29/49172 ,  H01L2224/85 ,  H01L2224/83 ,  H01L2924/00012 ,  H01L2924/00 ,  H01L2224/45015 ,  H01L2924/207

摘要： After conductive patterns are formed on the conductive foil every block by employing isolation trenches, conductive plating layers are arranged selectively on the conductive patterns. Therefore, it is possible to accomplish the circuit device manufacturing method by which the die bonding of the circuit elements can be applied stably and the wire bonding can also be applied stably and which can fit to the mass-production while saving the resource.

摘要翻译： 在通过采用隔离沟槽在每个块上在导电箔上形成导电图案之后，导电镀层选择性地布置在导电图案上。 因此，可以实现能够稳定地施加电路元件的管芯接合的电路器件的制造方法，并且还可以稳定地应用引线接合，并且可以适应批量生产，同时节省资源。

公开(公告)号：US4564557A

公开(公告)日：1986-01-14

申请号：US719429

申请日：1985-04-03

申请人： Masuhito Ohgushi ,  Hiroshi Ohtsuka ,  Tsutomu Asaba ,  Hironobu Hoshi ,  Kenichi Goshokubo ,  Takeo Wakui ,  Kiminori Ishii ,  Masashi Ohno ,  Yukio Okada

发明人： Masuhito Ohgushi ,  Hiroshi Ohtsuka ,  Tsutomu Asaba ,  Hironobu Hoshi ,  Kenichi Goshokubo ,  Takeo Wakui ,  Kiminori Ishii ,  Masashi Ohno ,  Yukio Okada

IPC分类号： C08F283/12 ,  C09D143/04 ,  C09D151/08 ,  B32B27/38 ,  B32B9/04 ,  C08F24/00 ,  C08F30/08

CPC分类号： C08F283/124 ,  C09D143/04 ,  C09D151/085 ,  Y10T428/12569 ,  Y10T428/261 ,  Y10T428/31511 ,  Y10T428/31663 ,  Y10T428/31699

摘要： A thermoset acrylic resin composition for coating metallic materials, particularly stainless steel, having a superior adhesion thereonto and hence a superior resistance to forming after coated, and a stainless steel piece coated with a coating material comprising the above composition, followed by baking, and having a superior colorability and endurability, are provided, which composition is obtained by radical-copolymerizing (a) an acrylic or methacrylic acid ester having 1-12 C in the ester moiety, in 40-80% by weight, (b) a hydroxyl group- or epoxy group-containing acrylic or methacrylic acid ester, in 20-5% by weight, (c) a vinyl group-containing polysiloxane in 20-0.01% by weight and (d) a vinyl group-containing silane compound in 20-2% by weight, these % by weight being based on the total weight of monomers (a)-(d).

摘要翻译： 一种用于涂覆金属材料，特别是不锈钢的热固性丙烯酸树脂组合物，其上具有优异的粘合性，因此具有优异的耐涂层性能，以及涂覆有包含上述组合物的涂料的不锈钢件，随后烘烤，并具有 提供了优异的着色性和耐久性，该组合物通过在（a）在酯部分中具有1-12个C的丙烯酸或甲基丙烯酸酯，（40-80重量％），（b）羟基 或含环氧基的丙烯酸或甲基丙烯酸酯，为20〜50重量％，（c）20〜0.01重量％的含乙烯基的聚硅氧烷，（d）20重量％的含乙烯基的硅烷化合物， 2重量％，这些重量％基于单体（a） - （d）的总重量。