公开(公告)号：US20060057613A1

公开(公告)日：2006-03-16

申请号：US11189546

申请日：2005-07-26

Applicant: Ramesh Ramakrishnan ,  Peter Riccelli

Inventor： Ramesh Ramakrishnan ,  Peter Riccelli

IPC: C12Q1/68 ,  C07H21/04

CPC classification number: C12Q1/689 ,  B82Y5/00 ,  B82Y10/00

Abstract: The present invention provides isolated oligonucleotides and methods for detecting a methicillin resistant Staphylococcus aureus in a sample, including a sample that comprises nucleic acid molecules of higher biological complexity than that of amplified nucleic acid molecules.

Abstract translation: 本发明提供了分离的寡核苷酸和用于检测样品中耐甲氧西林金黄色葡萄球菌的分离的寡核苷酸和方法，包括含有比扩增的核酸分子的生物学复杂性更高的核酸分子的样品。

公开(公告)号：US20140186827A1

公开(公告)日：2014-07-03

申请号：US13977629

申请日：2011-05-16

Applicant: Martin Pieprzyk ,  Robert C. Jones ,  Kenneth J. Livak ,  Andrew May ,  Alain Mir ,  Jian Qin ,  Ramesh Ramakrishnan ,  Sandra Spurgeon ,  Jun Wang ,  Bernhard G. Zimmermann

Inventor： Martin Pieprzyk ,  Robert C. Jones ,  Kenneth J. Livak ,  Andrew May ,  Alain Mir ,  Jian Qin ,  Ramesh Ramakrishnan ,  Sandra Spurgeon ,  Jun Wang ,  Bernhard G. Zimmermann

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/6855 ,  C12Q1/6858 ,  G01N33/542 ,  G01N33/582 ,  G01N33/6893 ,  G01N2800/368 ,  C12Q2521/301 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2535/131 ,  C12Q2537/143 ,  C12Q2537/16 ,  C12Q2563/173

Abstract: The present invention provides amplification-based methods for detection of genotype, mutations, and/or aneuploidy. These methods have broad applicability, but are particularly well-suited to detecting and quantifying target nucleic acids in free fetal DNA present in a maternal bodily fluid sample.

Abstract translation: 本发明提供用于检测基因型，突变和/或非整倍体的基于扩增的方法。 这些方法具有广泛的适用性，但是特别适合于检测和定量存在于母体体液样品中的游离胎儿DNA中的靶核酸。

公开(公告)号：US5808566A

公开(公告)日：1998-09-15

申请号：US494198

申请日：1995-06-23

Applicant: David A. Behr ,  Ramesh Ramakrishnan

Inventor： David A. Behr ,  Ramesh Ramakrishnan

IPC: G01C21/00 ,  G01C21/34 ,  G01C21/36 ,  G08G1/09 ,  G08G1/0967 ,  G08G1/0968 ,  G08G1/0969 ,  G08G1/123

CPC classification number: G08G1/096811 ,  G01C21/26 ,  G01C21/32 ,  G01C21/3626 ,  G01C21/3679 ,  G08G1/096716 ,  G08G1/096741 ,  G08G1/096775 ,  G08G1/096816 ,  G08G1/096822 ,  G08G1/096838 ,  G08G1/096844 ,  G08G1/096861 ,  G08G1/096872 ,  G08G1/096883 ,  G08G1/096894

Abstract: The invention provides a method and system for providing route guidance and other information from a base unit to a remote unit in response to a request from the remote unit. A query is formatted at the remote unit, the query including the request, and is transmitted from the remote unit to the base unit. Requested route guidance information is calculated at the base unit in response to the query, using a large up-to-date database located at the base unit. A response to the query is formatted at the base unit, the response including route guidance information. The response is then transmitted from the base unit to the remote unit for display. The transmission is made in a compact form through the use of maneuver arms and combined maneuver arms and through the use of tokenized forms. A maneuver arm represents a road at an intersection, for depiction on a display, by one or two endpoint coordinates. The tokenized forms are expanded at the remote unit into textual driving instructions for each of one or more languages. In addition, the amount of information available at a remote unit can be increased by providing the remote unit with information from the base unit which is not adequately covered by any databases on-board the remote unit.

公开(公告)号：US09487822B2

公开(公告)日：2016-11-08

申请号：US12170414

申请日：2008-07-09

Applicant: Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

Inventor： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

IPC: G01N33/483 ,  C12Q1/68 ,  G01N33/00 ,  B01L3/00 ,  G01N35/00

CPC classification number: C12Q1/6837 ,  B01L3/5027 ,  G01N35/00594 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2537/16 ,  C12Q2537/165

Abstract: A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules.

Abstract translation: 估计生物样品中DNA分子浓度的方法包括将多个反应位点存储在存储器中并在多个反应位点之间分配生物样品。 该方法还包括确定多个反应位点的数目，其特征在于存在一个或多个DNA分子，并计算以一个或多个DNA分子存在为特征的多个反应位点的一部分。 该方法还包括估计DNA分子的浓度作为多个反应位点的一部分的函数，并计算DNA分子的估计浓度的置信区间。

公开(公告)号：US20100203538A1

公开(公告)日：2010-08-12

申请号：US12695010

申请日：2010-01-27

Applicant: Simant Dube ,  Alain Mir ,  Ramesh Ramakrishnan ,  Lesley Suzanne Weaver ,  Bernhard G. Zimmermann

Inventor： Simant Dube ,  Alain Mir ,  Ramesh Ramakrishnan ,  Lesley Suzanne Weaver ,  Bernhard G. Zimmermann

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2561/113 ,  C12Q2545/10 ,  C12Q2527/107

Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.

Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。

公开(公告)号：US20090239308A1

公开(公告)日：2009-09-24

申请号：US12170414

申请日：2008-07-09

Applicant: Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

Inventor： Simant Dube ,  Jian Qin ,  Ramesh Ramakrishnan

IPC: G01N33/483 ,  C12M1/34

CPC classification number: C12Q1/6837 ,  B01L3/5027 ,  G01N35/00594 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2537/16 ,  C12Q2537/165

Abstract: A method of estimating a concentration of DNA molecules in a biological sample includes storing a number of a plurality of reaction sites in a memory and distributing the biological sample among the plurality of reaction sites. The method also includes determining a number of the plurality of reaction sites characterized by a presence of one or more of the DNA molecules and computing a portion of the plurality of reaction sites characterized by the presence of the one or more of the DNA molecules. The method further includes estimating the concentration of the DNA molecules as a function of the portion of the plurality of reaction sites and computing a confidence interval for the estimated concentration of DNA molecules.

Abstract translation: 估计生物样品中DNA分子浓度的方法包括将多个反应位点存储在存储器中并在多个反应位点之间分配生物样品。 该方法还包括确定多个反应位点的数目，其特征在于DNA分子中存在一个或多个DNA分子，并计算以一个或多个DNA分子存在为特征的多个反应位点的一部分。 该方法还包括估计DNA分子的浓度作为多个反应位点的一部分的函数，并计算DNA分子的估计浓度的置信区间。

公开(公告)号：US08697363B2

公开(公告)日：2014-04-15

申请号：US12548132

申请日：2009-08-26

Applicant: Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

Inventor： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay. In certain embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a sample-specific nucleotide tag and a target nucleotide sequence. In some embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a first nucleotide tag linked to a target nucleotide sequence, which is linked to a second nucleotide tag. In either case, the tagged target nucleotide sequences from the S samples can be mixed to form an assay mixture and subsequently assayed.

Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。 在某些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括样品特异性核苷酸标签和靶核苷酸序列。 在一些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括与靶核苷酸序列连接的第一个核苷酸标签，其连接到第二个 核苷酸标签。 在任一情况下，可以将来自S样品的标记的靶核苷酸序列混合以形成测定混合物并随后测定。

公开(公告)号：US08473216B2

公开(公告)日：2013-06-25

申请号：US11948782

申请日：2007-11-30

Applicant: Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

Inventor： Gang Sun ,  Ramesh Ramakrishnan ,  Robert C. Jones

IPC: G01N33/48

CPC classification number: C12Q1/686 ,  C12Q2565/629 ,  C12Q2565/501 ,  C12Q2545/114

Abstract: A method of adjusting amplification curves in a PCR experiment includes receiving a plurality of amplification curves for a sample and computing a first parameter for each of the plurality of amplification curves. The method also includes computing a second parameter for each of the plurality of amplification curves and computing a third parameter using at least a portion of the first or second parameters. The method further includes computing an offset for each of the plurality of amplification curves. The offset is a function of the first parameter and the third parameter. Moreover, the method includes adjusting at least one of the plurality of amplification curves by subtracting the offset.

Abstract translation: 在PCR实验中调整扩增曲线的方法包括接收样品的多个放大曲线并计算多个放大曲线中的每一个的第一参数。 该方法还包括为多个放大曲线中的每一个计算第二参数，并使用第一或第二参数的至少一部分来计算第三参数。 该方法还包括计算多个放大曲线中的每一个的偏移量。 偏移量是第一个参数和第三个参数的函数。 此外，该方法包括通过减去偏移来调整多个放大曲线中的至少一个。

公开(公告)号：US08450063B2

公开(公告)日：2013-05-28

申请号：US12695010

申请日：2010-01-27

Applicant: Simant Dube ,  Alain Mir ,  Ramesh Ramakrishnan ,  Lesley Suzanne Weaver ,  Bernhard G. Zimmermann

Inventor： Simant Dube ,  Alain Mir ,  Ramesh Ramakrishnan ,  Lesley Suzanne Weaver ,  Bernhard G. Zimmermann

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6853 ,  C12Q1/6809 ,  C12Q1/6886 ,  C12Q2600/16 ,  C12Q2561/113 ,  C12Q2545/10 ,  C12Q2527/107

Abstract: The present invention provides for determining relative copy number difference for one or more target nucleic acid sequences between a test sample and a reference sample or reference value derived therefrom. The methods facilitate the detection of copy number differences less than 1.5-fold.

Abstract translation: 本发明提供了确定测试样品和参考样品之间的一个或多个目标核酸序列的相对拷贝数差异或其衍生的参考值。 该方法有助于检测小于1.5倍的拷贝数差异。

公开(公告)号：US08148078B2

公开(公告)日：2012-04-03

申请号：US12206664

申请日：2008-09-08

Applicant: Ramesh Ramakrishnan

Inventor： Ramesh Ramakrishnan

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6869 ,  C12Q1/6851 ,  C12Q2545/101 ,  C12Q2527/143

Abstract: The present invention methods and systems for determining copy number variation of a target polynucleotide in a genome of a subject including amplification based techniques. Methods can include pre-amplification of the sample followed by distribution of sample and a plurality of reaction volumes, quantitative detection of a target polynucleotide and a reference polynucleotide, and analysis so as to determine the relative copy number of the target polynucleotide sequence in the genome of the subject.

Abstract translation: 用于确定受试者的基因组中的靶多核苷酸的拷贝数变异的本发明的方法和系统，包括基于扩增的技术。 方法可以包括样品的预扩增，随后分布样品和多个反应体积，定量检测靶多核苷酸和参考多核苷酸，并进行分析，以确定基因组中靶多核苷酸序列的相对拷贝数 的主题。