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    Microfluidic large scale integration
    4.
    发明授权
    Microfluidic large scale integration 有权
    微流控大规模集成

    公开(公告)号：US08220494B2

    公开(公告)日：2012-07-17

    申请号：US10915960

    申请日：2004-08-10

    申请人： Vincent Studer ,  Stephen R. Quake ,  W. French Anderson ,  Sebastian J. Maerkl

    发明人： Vincent Studer ,  Stephen R. Quake ,  W. French Anderson ,  Sebastian J. Maerkl

    IPC分类号： F15C1/06

    CPC分类号： F15C1/06 ,  G06F17/5018 ,  G06F2217/16 ,  Y10T137/2224

    摘要： Using basic physical arguments, a design and method for the fabrication of microfluidic valves using multilayer soft lithography is presented. Embodiments of valves in accordance with the present invention feature elastomer membrane portions of substantially constant thickness, allowing the membranes to experience similar resistance to an applied pressure across their entire width. Such on-off valves fabricated with upwardly- or downwardly-deflectable membranes can have extremely low actuation pressures, and can be used to implement active functions such as pumps and mixers in integrated microfluidic chips. Valve performance was characterized by measuring both the actuation pressure and flow resistance over a wide range of design parameters, and comparing them to both finite element simulations and alternative valve geometries.

    摘要翻译： 使用基本的物理参数，提出了使用多层软光刻制造微流体阀的设计和方法。 根据本发明的阀的实施例具有基本上恒定厚度的弹性体膜部分，允许膜在其整个宽度上经受类似的施加压力的阻力。 用向上或向下可偏转的膜制造的这种开关阀可以具有非常低的致动压力，并且可以用于实现积分功能，例如集成微流体芯片中的泵和混合器。 阀性能的特征在于在宽范围的设计参数下测量致动压力和流动阻力，并将其与有限元模拟和替代阀几何形状进行比较。

    Microfluidic nucleic acid analysis
    5.
    发明授权
    Microfluidic nucleic acid analysis 有权
    微流控核酸分析

    公开(公告)号：US08871446B2

    公开(公告)日：2014-10-28

    申请号：US10678946

    申请日：2003-10-02

    申请人： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    发明人： Jong Wook Hong ,  Vincent Studer ,  W. French Anderson ,  Stephen R. Quake ,  Jared Leadbetter

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  B01L3/00

    CPC分类号： B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2300/0809 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q2565/629

    摘要： Nucleic acid from cells and viruses sampled from a variety of environments may purified and expressed utilizing microfluidic techniques. In accordance with one embodiment of the present invention, individual or small groups of cells or viruses may be isolated in microfluidic chambers by dilution, sorting, and/or segmentation. The isolated cells or viruses may be lysed directly in the microfluidic chamber, and the resulting nucleic acid purified by exposure to affinity beads. Subsequent elution of the purified nucleic acid may be followed by ligation and cell transformation, all within the same microfluidic chip. In one specific application, cell isolation, lysis, and nucleic acid purification may be performed utilizing a highly parallelized microfluidic architecture to construct gDNA and cDNA libraries.

    摘要翻译： 来自从各种环境取样的细胞和病毒的核酸可以利用微流体技术纯化和表达。 根据本发明的一个实施方案，可通过稀释，分选和/或分割在微流体室中分离单个或小组的细胞或病毒。 分离的细胞或病毒可以直接在微流体室中裂解，并且通过暴露于亲和珠来纯化得到的核酸。 随后洗脱纯化的核酸之后可以连接和细胞转化，全部在相同的微流体芯片内。 在一个具体应用中，细胞分离，裂解和核酸纯化可以使用高度并行的微流体结构来构建gDNA和cDNA文库。

    Microfluidic system for controlling the concentration of molecules for stimulating a target
    6.
    发明授权
    Microfluidic system for controlling the concentration of molecules for stimulating a target 有权
    用于控制分子浓度用于刺激靶的微流体系统

    公开(公告)号：US09164083B2

    公开(公告)日：2015-10-20

    申请号：US14113136

    申请日：2012-04-20

    申请人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    发明人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    IPC分类号： G01N33/50 ,  G01N21/63 ,  B81B1/00 ,  B01L3/00 ,  C12M3/06

    CPC分类号： G01N33/5008 ,  B01J2219/00639 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/163 ,  C12M23/16

    摘要： The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device (1) comprising: n≧1 microfluidic channel(s) (4, 40), the or each channel being provided with at least one inlet orifice for at least one fluid and with at least one outlet orifice for this fluid; n≧2 openings (47, 470) formed in the microfluidic channel or distributed in the various microfluidic channels, said openings being arranged in one and the same plane so that they form a network having at least one dimension in this plane, the numbers n of microfluidic channel(s) and n of openings being linked by the relationship (I) with 1≦i≦n and n the number of openings for the channel c; at least one microporous membrane (5) covering the network of openings, the target being intended to be positioned on the side of the membrane which is opposite the microfluidic channel(s); one or more fluid feed means for feeding the or each microfluidic channel with fluid, at least one of these fluids comprising molecules for stimulating the target.

    摘要翻译： 本发明涉及一种用于控制卡的浓度的微流体系统，该分子能够刺激靶，例如通过活细胞组装形成，其特征在于该系统包括微流体装置（1），其包括：n≥1微流体 通道（4,40），该通道或每个通道设置有用于至少一种流体的至少一个入口孔口和用于该流体的至少一个出口孔口; n≥2个开口（47,470），其形成在微流体通道中或分布在各种微流体通道中，所述开口布置在同一平面中，使得它们形成在该平面中具有至少一个维度的网络，数字n 的微流体通道和n个开口通过关系式（I）与1＆nlE; i＆nlE; n和n相关联，n是通道c的开口数; 覆盖开口网络的至少一个微孔膜（5），所述靶旨在位于所述膜的与所述微流体通道相对的一侧上; 一个或多个流体供给装置，用于将流体或每个微流体通道供给流体，这些流体中的至少一个包含用于刺激靶的分子。

    MICROFLUIDIC SYSTEM FOR CONTROLLING A CONCENTRATION PROFILE OF MOLECULES CAPABLE OF STIMULATING A TARGET
    7.
    发明申请
    MICROFLUIDIC SYSTEM FOR CONTROLLING A CONCENTRATION PROFILE OF MOLECULES CAPABLE OF STIMULATING A TARGET 有权
    用于控制能够刺激目标的分子浓度分布的微流控系统

    公开(公告)号：US20140080206A1

    公开(公告)日：2014-03-20

    申请号：US14003206

    申请日：2012-03-02

    申请人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    发明人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5008 ,  B01J2219/00639 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/163 ,  C12M23/16

    摘要： The invention relates to a microfluidic system for controlling a concentration profile of molecules capable of stimulating a target, for example formed by an assembly of living cells, this system comprising: -a microfluidic device (1) comprising at least one microfluidic channel (4) equipped with at least one inlet orifice (21) and with at least one outlet orifice (22) for at least one fluid; -at least one means for supplying the microfluidic channel (4) with at least one fluid comprising molecules capable of stimulating the target; -at least one chamber (8) or another microfluidic channel comprising a base (6) intended to receive the target; and -at least one microporous membrane (5) separating the chamber (8) or the other microfluidic channel from the microfluidic channel (4), said microporous membrane (5) being positioned away from the base (6) so that when the supply means provides the microfludic channel (4) with said at least one fluid flowing in laminar flow in contact with the microporous membrane (5), the molecules capable of stimulating the target then diffuse, after having passed through the microporous membrane (5) through the chamber (8) or said other microfluidic channel in order to finally form a stable concentration profile in this chamber (8) or this other microfluidic channel.

    摘要翻译： 本发明涉及用于控制能够刺激靶的分子的浓度分布的微流体系统，例如通过活细胞的组装形成，该系统包括： - 微流体装置（1），其包含至少一个微流体通道（4） 装备有至少一个入口孔口（21）和至少一个用于至少一种流体的出口孔口（22）; - 至少一种用于向所述微流体通道（4）供应至少一种包含能够刺激所述靶的分子的流体的装置; - 至少一个腔室（8）或另一个微流体通道，其包括用于接收靶的基部（6） 以及 - 将所述腔室（8）或所述另一个微流体通道与所述微流体通道（4）分开的至少一个微孔膜（5），所述微多孔膜（5）远离所述基座（6）定位，使得当所述供给装置 提供微流体通道（4）与所述至少一种流体在微流体中与微多孔膜（5）接触流动的流体，然后在经过微孔膜（5）通过腔室之后，能够刺激靶的分子扩散 （8）或所述其它微流体通道，以便最终在该腔室（8）或该另一微流体通道中形成稳定的浓度分布。

    MICROFLUIDIC SYSTEM FOR CONTROLLING THE CONCENTRATION OF MOLECULES FOR STIMULATING A TARGET
    8.
    发明申请
    MICROFLUIDIC SYSTEM FOR CONTROLLING THE CONCENTRATION OF MOLECULES FOR STIMULATING A TARGET 有权
    用于控制用于刺激目标的分子浓度的微流控系统

    公开(公告)号：US20140113366A1

    公开(公告)日：2014-04-24

    申请号：US14113136

    申请日：2012-04-20

    申请人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    发明人： Maxime Dahan ,  Mathieu Morel ,  Jean-Christophe Galas ,  Vincent Studer ,  Denis Bartolo

    IPC分类号： G01N33/50

    CPC分类号： G01N33/5008 ,  B01J2219/00639 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2300/0627 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0848 ,  B01L2300/0851 ,  B01L2300/087 ,  B01L2300/163 ,  C12M23/16

    摘要： The invention relates to a microfluidic system for controlling a card for the concentration of molecules capable of stimulating a target, for example formed by an assembly of living cells, characterized in that the system comprises a microfluidic device (1) comprising: n≧1 microfluidic channel(s) (4, 40), the or each channel being provided with at least one inlet orifice for at least one fluid and with at least one outlet orifice for this fluid; n≧2 openings (47, 470) formed in the microfluidic channel or distributed in the various microfluidic channels, said openings being arranged in one and the same plane so that they form a network having at least one dimension in this plane, the numbers n of microfluidic channel(s) and n of openings being linked by the relationship (I) with 1≦i≦n and n the number of openings for the channel c; at least one microporous membrane (5) covering the network of openings, the target being intended to be positioned on the side of the membrane which is opposite the microfluidic channel(s); one or more fluid feed means for feeding the or each microfluidic channel with fluid, at least one of these fluids comprising molecules for stimulating the target.

    摘要翻译： 本发明涉及一种用于控制卡的浓度的微流体系统，该分子能够刺激靶，例如通过活细胞组装形成，其特征在于该系统包括微流体装置（1），其包括：n≥1微流体 通道（4,40），该通道或每个通道设置有用于至少一种流体的至少一个入口孔口和用于该流体的至少一个出口孔口; n≥2个开口（47,470），其形成在微流体通道中或分布在各种微流体通道中，所述开口布置在同一平面中，使得它们形成在该平面中具有至少一个维度的网络，数字n 的微流体通道和n个开口通过关系式（I）与1＆nlE; i＆nlE; n和n相关联，n是通道c的开口数; 覆盖开口网络的至少一个微孔膜（5），所述靶旨在位于所述膜的与所述微流体通道相对的一侧上; 一个或多个流体供给装置，用于将流体或每个微流体通道供给流体，这些流体中的至少一个包含用于刺激靶的分子。

    Microfluidic large scale integration
    10.
    发明申请
    Microfluidic large scale integration 有权
    微流控大规模集成

    公开(公告)号：US20050072946A1

    公开(公告)日：2005-04-07

    申请号：US10915960

    申请日：2004-08-10

    申请人： Vincent Studer ,  Stephen Quake ,  W. Anderson ,  Sebastian Maerkl

    发明人： Vincent Studer ,  Stephen Quake ,  W. Anderson ,  Sebastian Maerkl

    IPC分类号： F16K31/00

    CPC分类号： F15C1/06 ,  G06F17/5018 ,  G06F2217/16 ,  Y10T137/2224

    摘要： Using basic physical arguments, a design and method for the fabrication of microfluidic valves using multilayer soft lithography is presented. Embodiments of valves in accordance with the present invention feature elastomer membrane portions of substantially constant thickness, allowing the membranes to experience similar resistance to an applied pressure across their entire width. Such on-off valves fabricated with upwardly- or downwardly-deflectable membranes can have extremely low actuation pressures, and can be used to implement active functions such as pumps and mixers in integrated microfluidic chips. Valve performance was characterized by measuring both the actuation pressure and flow resistance over a wide range of design parameters, and comparing them to both finite element simulations and alternative valve geometries.

    摘要翻译： 使用基本的物理参数，提出了使用多层软光刻制造微流体阀的设计和方法。 根据本发明的阀的实施例具有基本上恒定厚度的弹性体膜部分，允许膜在其整个宽度上经受类似的施加压力的阻力。 用向上或向下可偏转的膜制造的这种开关阀可以具有非常低的致动压力，并且可以用于实现积分功能，例如集成微流体芯片中的泵和混合器。 阀性能的特征在于在宽范围的设计参数下测量致动压力和流动阻力，并将其与有限元模拟和替代阀几何形状进行比较。